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장기적 트립토판 결여식이 생쥐의 유해감각예민도에 미치는 영향
서영대,박제민,김명정,전명호,양구범,장세헌,강철중,김정태,김용식 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.1
The depressive patients can be divided into two subgroups by presence and absence of pain symptoms. Those without pain symptoms have blunted pain sensitivity whereas those with pain have normal range of pain sensitivity, and are clinically characterized by anxiety and irritability. The aim of the study is to test if these clinical profiles of the depressives with pain symptoms are related with reduced level of brain serotonin(5-HT). Forty four mice were randomly divided into two groups : one group to be bred with tryptophan free diet and the other with normal control diet, each for 4 weeks. Before the beginning of the breeding period, measured were locomotor activity by open field test, anxiety by elevated plus maze and nociceptive sensitivity by tail flick test, before and after forced swimming(FS). During each FS, duration of immobilization was also measured. The sable sets of measurements were repeated at the end of the breeding period. The brain 5-HT and 5-hydroxyindoleacetic acid(5-HIAA) were measured by high performance liquid chromatography. The results were as follows 1.Four weeks of tryptophan free diet reduced significantly body weight, brain weight, and levels of 5-HT and 5-HIAA in the whole brain. 2.Tryptophan depletion did not influence basal nociceptive sensitivity as measured by tail flick latency before FS. The normal blunting of the pain sensitivity induced by forced swimming was preserved in the tryptophan depleted group. 3.Tryptophan depletion did not influence general locomotor activity in open field. 4.Tryptophan depletion increased significantly time spent on the open arms at the elevated plus maze test done before FS. This anxiolytic-like effect was reduced by FS. 5.Tryptophan depletion did not influence duration of immobilization during FS. From these results, it is suggested that the genesis of the depression with pain symptoms is not medicated by quantitative reduction of brain serotonin
화상 환자에서 발생한 외과적 중재술을 요하는 감염성 심내막염 2예
김근숙,이태유,정연손,최창순,최민호,류제헌,김철홍,조구영,우흥정 대한감염학회 2003 감염과 화학요법 Vol.35 No.3
감염성 심내막염은 화상 후에 발생할 수 있는 치명적인 합병증이다. 임상 증상은 일반적으로 없는 경우가 대부분이고 지속되는 발열과 양성 혈액 배양 검사가 유일하다. 감염의 다른 확실한 원인 없이 발열과 양성 혈액 배양 검사가 있을 때는 감염성 심내막염을 강력하게 의심해야 하며 심장 초음파로 진단 가능하다. 감염성 심내막염은 사망률은 높지만 초기에 진단하면 항생제 정주를 지속하는 것으로 치료할 수 있다. 저자들은 화상환자에서 발생한 수술적 처치를 필요로 하는 감염성 심내막염 2예를 경험하였기에 보고하는 바이다. Infective endocarditis is a rare but fatal complication following burn injury. The clinical presentation is silent, but with persistent fever and positive blood culture. The manipulation of clinical care as well as the burn wound itself and immunosuppression caused by extensive bums puts the burn patients at risk of bacteremia. Staphylococcus aureus and Gram-negative bacilli are most common pathogens of infective endocarditis following burns. We report herein two cases of infective endocarditis in the burn patients who requires surgical intervention. The first case was caused by Pseudomonas aeruginosa with complication of multifocal pneumonia, and the second case by coagulase-negative stapylococcus with cerebral hemorrhage.
Jong Cheol Kim,Se Jin Lee,Seung Ho Yun,Sang Sick Lee,Chae Hoon Im,Jae Young Choi,Yeon Ho Je,Nam Jung Kim,Jae Su Kim 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04
Mealworms, Tenebrio molitor (L.) is used as an important animal feed additive for growth promotion and health management, but potentially exposes to fungal infection. In this work, virulence of two species of entomopathogenic fungi against the insect, and the relationship between abiotic features and virulence were investigated. Secondly our consideration was also given to the effect of chemical fungicides on conidial germination for risk control. Between Beauveria bassiana (Bb) and Metarhizium roberstii (Mr) (previously M. anisopliae), Bb isolates had much higher virulence (~100% mortality in 3~4 days after the treatment), rather than Mr isolates in laboratory assays. Next, fungus-treated mealworms were kept at wheat bran at 20, 25, 30 and 35℃ with 3, 6, 9 times of water spray to the feeds for set-up of different humidity conditions. Inoculation of fungi to mealworms was conducted by fungal spray and feeding methods, which resulted in higher virulence in feeding method. In the feeding method, all temperature treatments except 35℃ showed high virulence against mealworms, but any significant relationship between virulence and humidity was not observed. In the chemical fungicide screening, fluazinam (CAS No. 79622-59-6) and mancozeb (8018-01-7) significantly inhibited the germination of Bb and Mr conidia. This work suggest that contamination of wheat bran with fungal pathogens, particularly B. bassiana may induce mycosis of mealworms, but introduction of effective fungicides possibly reduce fungal infection.
염충호 ( Yeom Chung Ho ),최기철 ( Choe Gi Cheol ),이종은 ( Lee Jong Eun ),정종훈 ( Jeong Jong Hun ),전제열 ( Jeon Je Yeol ),윤평진 ( Yun Pyeong Jin ),염철호 ( Yeom Cheol Ho ) 대한신장학회 2001 Kidney Research and Clinical Practice Vol.20 No.6
2-kidney, 1 clip(2K1C) 신혈관성 고혈압 동물에서 생체내 내원성 산화질소(nitric oxide, NO)에 의한 혈관확장성 긴장이 변화되는가 밝히고자 NG-nitro-L-arginine(L-NNA), lipopolysaccharide(LPS) 및 tempol을 투여하여 혈압변화 및 혈장 NO 함량을, 그리고 적출 동맥환표본의 장력반응과 NO synthase(NOS) 단백발현을 조사하여 다음과 같은 결과를 얻었다. 1) L-NNA는 2K1C 고혈압군과 대조군에서 혈압을 상승시켰으며 그 정도는 양군에서 유사하였다. 2) LPS는 고혈압군과 대조군에서 혈압을 하강시켰고 그 정도는 양군에서 유사하였으며 고혈압군은 시간이 경과함에 따라 혈압이 회복된 양상을 보였다. 3) Tempol은 고혈압군에서 서서히 혈압을 하강시켰으나 대조군에서는 혈압변화를 일으키지 않았다. 4) LPS 투여 후 혈장 NO 함량은 고혈압군과 대조군에서 각각 유의하게 증가되었으며 양군간의 차이는 볼 수 없었다. 5) 혈장 NO 함량은 고혈압군과 대조군에서 tempol에 의해 영향 받지 않았다. 6) L-NNA에 의한 혈관수축반응은 고혈압군에서 대조군에 비해 유의하게 약화되었다. 7) 혈관에서 eNOS 및 iNOS 단백발현은 고혈압군에서 대조군에 비해 각각 유의하게 낮았다. 이상의 실험결과로 보아 2K1C 신혈관성 고혈압에서 생체내 NO의 총 혈관확장성 긴장은 변하지 않으나 혈관에서의 NO 긴장은 감소될 것으로 사료된다. Endogenous nitric oxide(NO) plays an important role in the regulation of blood pressure. It has been known that the evoked NO-dependent dilator system may be impaired in various hypertensive models. The effects of NG-nitro-L-arginine(L-NNA), lipopolysaccharide( LPS) and tempol on mean arterial pressure( MAP) and the effects of L-NNA on isolated aorta tone were studied in order to elucidate potential alterations in resting vasodilator tone of NO in two-kidney, one clip(2K1C) hypertension. Plasma nitrite/ nitrate levels were measured by colorimetric assay, and the expression of endothelial and inducible NO synthases(eNOS, iNOS) was determined by Western blot analysis. L-NNA caused an increase of MAP, while LPS produced a hypotensive effect in both 2K1C and control rats. The magnitude of the pressor or depressor response to L-NNA and LPS was comparable in the two groups. Tempol induced a sustained decrease in MAP in 2K1C rats, while it had no effects on MAP in control rats. Plasma concentrations of NO metabolites were significantly increased following the LPS-treatment in both 2K1C and control rats, while they were not affected by tempol-treatment. In endothelium-intact aortic rings precontracted with 25 mM KCl, L-NNA caused a dose-dependent contraction. The magnitude of the maximal contraction was attenuated in 2K1C rats as compared with control. An inhibition of contractile responses to L-NNA in the hypertensive group was also shown in rubbed rings, although the magnitude of contractions was markedly reduced. The vascular expression of both eNOS and iNOS was significantly decreased in 2K1C rats as compared with control. These results indicate that 2K1C hypertension is associated with a reduced basal vasodilator tone of NO and a decrease in the vascular expression of NOS isozymes.
( Cheol Su Kim ),( Ho Sup Lee ),( Chang Ki Min ),( Je Jung Lee ),( Ki Hyun Kim ),( Dok Hyun Yoon ),( Hyeon Seok Eom ),( Hyewon Lee ),( Won Sik Lee ),( Ho Jin Shin ),( Ji Hyun Lee ),( Young Park ),( Ja 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Many prognostic factors have been studied with development of treatment in MM. However, serologic infiammatory markers such as serum beta2-microglobulin (B2MG), albumin, absolute lymphocyte count(ALC), C-reactive protein (CRP), lactate dehydrogenase (LDH), serum ferritin level which were easily tested in patients to find disease status have been reported that these were correlated with prognosis in many studies, respectively. Therefore, the purpose of this study is to estimate correlation between frequency of combined infiammatory parameters such as ALC, LDH, B2MG, albumin, CRP, and ferritin and prognosis for survival in patients with MM treated with induction chemotherapy containing thalidomide underwent autologous stem cell transplantation (ASCT). Methods: Data from patients at thirteen university hospitals in South Korea between December 2005 and May 2013 were collected retrospectively. All included patients were treated with thalidomide containing chemotherapy and then underwent ASCT. The infiammatory score was defined that each infiammatory parameters including ALC level under 1000/μL, Serum B2MG level above 3.5 mg/L Serum albumin under 3.5 g/dL and LDH above 450 IU/L were scored 1 point and sum of those were used infiammatory scores such as 0 to 4 points. Results: In the multivariate analysis, Infiammatory score (<2) was only independent prognostic factor for superior PFS (RR;0.618, 95% C.I. 0.409-0.933, p=0.022) and platelet counts of more than 100×109/L, infiammatory score of less than 2 were independent prognostic factors for superior OS (RR 7.856, 95% C.I. 2.502-24.670, p<0.001 and RR 0.216, 95% C.I. 0.067-0.696, p=0.010, respectively). Conclusions: Patients combined many infiammatory factors who treated with thalidomide induction chemotherapy underwent ASCT were shown significant short survival and these results might be helped to predict prognosis.
( Je Hyun Seo ),( Ki Ho Park ),( Yu Jeong Kim ),( Young Cheol Yoo ),( Shin Hee Kang ),( Dong Myung Kim ) 대한안과학회 2008 Korean Journal of Ophthalmology Vol.22 No.1
Purpose: To investigate the differences in the histopathology and matrix metalloproteinase (MMP) expression in the Tenon`s tissue of primary open-angle glaucoma (POAG) patients, primary angle-closure glaucoma (PACG) patients, and non-glaucomatous patients. Methods: POAG and PACG patients, who underwent a trabeculectomy and had no history of ocular disease except glaucoma, were enrolled. The number and instillation period of topical eye drops were reviewed. For the controls, which were patients without glaucoma or a history of ocular surgery, the Tenon`s tissue was obtained in the course of retinal detachment surgery. For glaucoma patients, the Tenon`s tissue was obtained during the trabeculectomy. H&E and Masson`s trichrome staining and immunohistochemistry for MMP-1, MMP-2, and MMP-9 were performed. A total of six eyes of POAG, six eyes of PACG, and four control eyes were evaluated. Results: The duration of topical anti-glaucoma medication and the mean number of anti-glaucoma medications were similar in the POAG and PACG groups. The levels of MMP-1 and 2 were elevated in the POAG and PACG groups compared to the control group (p=0.03, 0.01, respectively). Compared with the control group, the MMP-2 level was higher in the POAG patients (p=0.01), whereas the MMP-1 was higher in the PACG patients (p=0.04). The levels of MMP-9 in the POAG and PACG patients were not significantly different from that of the control patients (p=0.48, 0.26). The levels of MMP-2 were significantly lower in the PACG patients than in the POAG patients (p=0.02). Conclusions: The MMP expression was altered in the Tenon`s tissue of glaucoma patients compared to the control group. The levels of MMP-2 were lower in the PACG patients than in the POAG patients. These results suggest that there may be histopathological differences in the Tenon`s tissue of POAG and PACG patients. Korean Journal of Ophthalmology 22(1):37-42, 2008