띠미로 버섯 추출물의 함암효과

양규호,양정희,류병호 한국산업미생물학회 1997 한국미생물·생명공학회지 Vol.25 No.2

본 연구는 옛날부터 생리활성이 우수한 것으로 알려진 띠미로 버섯(Daedalea dickinsii)에 대한 항암효과를 알아보기 위하여 열수 및 메타놀 추출물로서 사람의 암세포인 Hela cell, Hep G_2 cell, L929 cell 및 동물의 육종 암세포인 sarcoma-180을 이용하여 실험하였다. 띠미로 버섯의 열수 추출물은 1.5 ㎎/100㎖ 농도에서 Hela cell에 대하여 70%의 높은 항암효과를 나타내었고, Hep G_2 cell은 40%정도의 항암효과를 나타내었다. 띠미로 버섯의 메타놀 추출물의 경우 4 ㎎/㎖농도에서 Hela cell에 대하여 60%의 억제효과를 나타내었다. L929 cell에 대한 띠미로 버섯의 열수 추출물은 50.03%의 억제효과를 나타내었다. Sarcoma-180에 대한 종양 억제효과는 띠미로버섯의 열수 추출물을 60 ㎎/㎏ 투여시 45.67%의 억제효과가 있었고 수명연장은 30.88%로서 대조군보다 높았다. This study was carried out particularly focusing on the antitumor effects of extracts obtained from mushroom, Daedalea dickinsii against Hela cell, Hep G_2 cell, L 929 cell and Sarcoma-180. Antitumor effect of hot water extracts obtained from Daedalea dickinsii against Hela cell showed at the highest level of 70% when administrated at the concnetration of 1.5 ㎎/100㎖, however Hep G_2 cell inhibited 40% at the same concentration of of hot water extracts. Antitumor effects of methanol extract of Daedalea dickinsii against Hela cell indicated at the highest level of 60% when 4 ㎎/㎖ was administerated. Antitumor effect of hot water extract of Daedalea dickinsii inhibited 51.03% against L929 cell. The solid tumor sarcoma-180 growth inhibition of methanol extract of Deadalea dickinsii inhibited 45.67% when administrated at the concentration of 60 ㎎/㎏, and the life prolongation was higher 30.88% than that control group.

정상교합자와 골격성 Ⅲ급 부정교합자의 경추골성숙도에 관한 연구

양규호,최남기,최봉선,이영준,류선열,김선미 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.1

정상교합자와 골격성 Ⅲ급 부정교합자간의 성장 정도에 차이가 있는지를 평가하기 위해 8-12세 여자를 대상으로 하여 진단 석고 모형과 측모두부방사선사진을 기준으로 정상교합자 172명 , 골격성 Ⅲ급 부정교합자 191명 총 363명을 분류하여 연구를 시행하였다. 경추의 하연의 만곡도와 수직·수평 길이의 비를 측정해서 연령이 증가함에 따른 변화를 분석하여 다음과 같은 결론을 얻었다. 1. 연령이 증가함에 따라 제2~6경추의 하연의 만곡도는 정상교합자와 골격성 Ⅲ급 부정교합자 모두에서 일정하게 증가하였다. 2. 연령이 증가함에 따라 제3~6경추의 수직·수평 길이의 비도 정상교합자와 골격성 Ⅲ급 부정교합자 모두에서 일정하게 증가하였다. 3. 제2~6경추의 하연의 만곡도와 제3~6경추의 수직·수평 길이의 비에서 정상교합자와 골격성 Ⅲ급 부정교합자간의 경추골성숙도 차이를 조사한 결과 통계적으로 유의한 차이를 보이지 않았다. 이상의 결과에서 정상교합자와 골격성 Ⅲ급 부정교합자간의 경추골성숙도를 비교했을 때 유의한 차이를 보이지 않았으므로 성장에 차이가 없음을 시사하였다. This study was to evaluate and compare differences of the cervical vertebral skeletal maturity of normal occlusion and skeletal Class Ⅲ malocclusion. Normal occlusion (172 girls) and skeletal Class Ⅲ malocclusion(191 girls) were classified according to diagnosis stone model and lateral cephalogram of Korean girls aging from 8 to 12 years. The concavity of inferior border, vertico-horizontal ratio of cervical vertebrae were observed and measured according to age. Differences of the cervical vertebral skeletal maturity were evaluated. The results were as follows : 1. The concavity of inferior border of the 2nd to 6th vertebrae of normal occlusion and skeletal Class Ⅲ had uniformly increased with age. 2. The vertico-horizontal ratio of the 3rd to 6th vertebrae of girls with normal occlusion and skeletal Class Ⅲ had uniformly increased with age. 3. There was no significant difference in cervical vertebral skeletal maturity between normal occlusion and skeletal Class Ⅲ malocclusion in the concavity of inferior border of the 2nd to 6th vertebrae and in the vertico-horizontal ratio of the 3rd to 6th vertebrae. The results in the study indicate that there is no significant difference of cervical vertebral skeletal maturity between girls with normal occlusion and skeletal Class Ⅲ malocclusion.

심미수복 재료의 마모와 화학적 분해

양규호,최남기,김훈주,김선미 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.2

수복재료의 요건으로서 치아의 저작기능과 심미성을 회복할 수 있는 물리적, 화학적 성질뿐만 아니라 생물학적 적합성과 구강내 환경변화에 대한 내구성을 들 수 있다. 불소 방출의 장점을 갖는 컴포머나 시술시간을 줄이는데 유리한 재료인 유동성 복합레진을 유구치부에 사용하려고 할 때 마모저항성과 구강내 환경에서의 분해저항성은 중요한 물성 중 하나이다. 실험에 사용된 복합레진은 최근에 시판되고 있는 Charmfil(Denkist, Korea)과 유동성인 Charmfil flow(Denkist, Korea)이고, 컴포머는 Compoglass F(Ivoclar Vivadent, Liechtenstein)와 유동성인 PrimaFlow(DMG Hamburg, Germany)이었다. 각 제품의 분해저항성과 마모도를 평가하고자 마모시험 후 마모된 면의 깊이를 측정하였고 알칼리성 용액에 보관 시 각 제품의 분해저항성을 무게손실, 표면하 분해층 깊이, 용출된 Si 농도를 기준으로 평가하였고 주사전자현미경과 공촛점 레이저 현미경으로 분해층을 관찰하여 다음과 같은 결과를 얻었다. 1. 무게손실량은 각 제품간 차이를 보이지 않았다(p>0.05). 2. 분해층 깊이는 Compoglass F가 가장 깊었고, PrimaFlow, Charmfil, Charmfil flow 순이었고 Compoglass P와 다른 제품 간에는 유의한 차이를 보였다(p<0.05). 3. Si 용출량은 Charmfil flow가 가장 많았고, Compoglass F가 가장 작았으며 두 제품 간에는 유의한 차이를 보였다(p<0.05). 4. 주사전자현미경 관찰시 표면 양상 및 분해층 깊이를 관찰할 수 있었고 공촛점 레이저 현미경 관찰시 NaOH 용액에 보관한 후 수복재의 기질과 충전제 사이의 결합의 파괴 양상인 분해층 깊이를 관찰할 수 있었다. 5. 마모는 Compoglass F에서 가장 많이 일어났으며, PrimaFlow, Charmfil, Charmfil flow 순이었고 각 제품 간에는 유의한 차이를 보였다.(p<0.05). 6. 각 제품의 Si 용출량과 분해층 깊이 사이(r=0.602, p<0.05), 마모 최대 깊이와 비커스 경도 사이(r=0.501, p<0.05)에는 유의한 상관관계를 보였으나 Si 용출량과 마모 최대 깊이 등 다른 항목간에는 유의한 상관관계를 보이지 않았다.(r=-0.052, p>0.05). 본 연구에서 Compoglass F는 불소 함량은 가장 높았으나 화학적 분해층과 마모깊이가 가장 깊은 것으로 나타났으며 flowable type의 복합레진과 컴포머는 표면 경도와 마모도에서 양호한 결과를 보였다. 이상의 결과 복합레진과 컴포머의 평가요소로서 마모도와 함께 가수분해도 고려되어야 할 것으로 사료된다. The aim of this study was to evaluate the resistance to degradation and to compare the wear resistance characteristics of four esthetic restorative materials in an alkaline solution. The brands studied were Charmfil, Charmfil flow(composite resin), Compoglass F and PrimaFlow(compomer) . The results were as follows: 1. The mass loss were not significantly different among the materials(p〉0.05). 2. The sequence of the degree of degradation layer depth was in descending order by Compoglass F, PrimaFlow. Charmfil, and Charmfil flow. There were significant differences between Compoglass F and the others(p<0.05). 3. The sequence of the Si loss was in descending order by Charmfil flow, Charmfil, PrimaFlow, and Compoglass F. There were significant differences among these materials(p<0.05). 4. When observed with SEM, destruction of bonding between matrix and filler was observed and when observed with CLSM, the depth of degradation layer of specimen surface was observed. 5. The sequence of maximum wear depth was in descending order by Comfoglass, PrimaFlow, Charmfil, and Charmfil flow. There were significant differences among these materials(p<0.05). 6. The correlation coefficient between Si loss and degradation layer depth (r=0.602, p〈0.05), Vicker's hardness number and maximum wear depth (r=0.501, p〈0.05) were relatively high. These results indicate that wear and hydrolytic degradation may be considered to be evaluation factors of composite resins and compomers.

구치부 레진 수복 재료의 가수분해

양규호,박미란,최남기,박은혜 大韓小兒齒科學會 2001 大韓小兒齒科學會誌 Vol.28 No.4

우수한 심미성 수복재료로서 복합레진은 그 사용 빈도가 증가하고 있다. 이런 증가 추세에도 불구하고 복합레진의 부적절한 마모저항성 때문에 구치부 수복에서의 사용이 제한되어왔다. 이와 관련된 인자로 수복물의 표면하 분해가 고려되고 있다. 본 연구에서는 복합레진의 마모에 미치는 환경적 분해의 효과를 알기 위해 알카리성 용액(0.1 N NaOH)에 현재 많이 사용되는 Definite(Degussa-Hu¨ls AG, Germany), Prodigy(Kerr, USA), Pyramid(Bisco, USA) 및 Synergy(Coltene, Swiss)등 4종의 복합레진을 보관하였을 때 각 제품의 분해과정을 평가하고자 하였다. 각 제품 당 3개의 시편을 제작한 후 0.1N NaOH 용액에 저장하여 60℃에서 보관하였다. 2주 후 제거하여 HCI로 중화, 세척 후 60℃에서 건조하였다. 무게 손실, 분해층 깊이, Si 농도 등을 기준으로 분해저항성을 평가하여 다음과 같은 결과를 얻었다. 1. 무게 손실은 Synergy에서 1.24±0.002%로 가장 높은 값을 보였으나 각 제품간 유의한 차이는 보이지 않았다. 2. 분해층 깊이는 Synergy에서 107.83±2.52㎛로 가장 높은 값을 보였고, Synergy를 제외한 다른 제품에서는 유의한 차이를 보이지 않았다. 3. Si 용출량에 있어서는 4가지 제품 모두 차이를 보이지 않았다. 4. 무게 손실과 분해층 깊이 사이에는 높은 상관 관계를 보였다(r=0.6127, p<0.05). 5. 무게 손실과 Si 용출량, 분해층 깊이와 Si 용출량 사이에 상관 관계는 없는 것으로 나타났다. 6. 주사전자현미경 관찰시 NaOH 용액에 보관한 후 레진 기질과 필러 사이의 결합 파괴를 관찰할 수 있었다. The use of resin composites has continued to increase over the last several years. In spite of their growing popularity, composites continue to exhibit a number of undesirable characteristics. One of the major deficiencies of composite restorative resins is their inadequate resistance to wear. Of the multitude of factors that have been associated with wear, subsurface degradation within the restoration is considered to be one. The aim of this study was to evaluate the resistance to degradation of four commercial composite resins in an alkaline solution. This solution with a high concentration of hydroxyl ions is a convenient medium for accelerated degradation of silane coupling and filler particles. The brands studies were Definite(Degussa-Hu¨ls AG, Germany), Prodigy(Kerr, USA), Pyramid(Bisco, USA) and Synergy(Coltene, Swiss). Preweighed discs of each brand were exposed to 0.1N NaOH solution at 60℃. After 14 days they were removed, neutralized with HCI, washed with water and dried. Resistance to degradation was evaluated on the basis of following parameters: (a) mass loss(%)-determined from pre-and post-exposed specimen weights; (b) Si loss(ppm)-obtained from ICP-AE analysis of solution exposed to specimens; and (c) degradation depth(㎛)-measured microscopically (SEM) from polished circular sections of exposed specimens. The results were follows: 1. Mass loss of Synergy was 1.24±0.002%. it was the highest, there was no significant difference among the materials. 2. The degree of degradation layer depth of Synergy was 107.83±2.52㎛, it was the highest, there was no significant difference among any other materials than Synergy. 3. There was no difference among the four materials in Si loss. 4. The correlation coefficient between mass loss and degradation depth was relatively high(r=0.06, p<0.05). 5. There was no coefficient correlation between Si loss and mass loss, the degree of degradation layer depth and Si loss. 6. When observed with SEM, destruction of bonding is observed between resin matrix and filler.

정상교합자와 Ⅲ급 부정교합자의 수완부 골성숙 단계와 치아석회화도에 대한 연구

정병초,양규호 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.2

To investigate the relationship between the calcification stages of mandibular canines and the skeletal maturity stage of the hand-wrist in subjects with normal occlusion and Class Ⅲ malocclusion, hand-wrist radiographs and panoramic radiographs were taken from subjects of normal occlusion(94 males,88 females) and Class Ⅲ malocclusion(75 males,76 females) who had no systemic diseases and no history of orthodontic or prosthodonic treatment. Fishman's method for the skeletal maturity stages of the hand-wrist and Demirijian's method for the calcification stages of mandibular canines were used and analyzed. The results were as follows: 1.In subjects with normal occlusion and Class Ⅲ malocclusion,skeletal maturity of the hand-wrist and calcification of mandibular canines at various ages occured earlier in females than males(P<0.05). 2.Comparing the skeletal maturity stages of the hand-wrist and calcification stages of mandibular canines between subjects with normal occlusion and Class Ⅲ malocclusion,there were no significant differences between the groups. 3.The correlation coefficient between the calcification stages of mandibular canines and the skeletal maturity stages of thehand-wrist in subjects with normal occlusion and Class Ⅲ malocclusion showed a high association(P<0.01). 4.In stage 4 of the skeletal maturity of the hand-wrist,the frequency distribution of calcification G stage among the various calcification stages highest both in normal occlusion and in subjects with Class Ⅲ malocclusion.However,there was no signicant differences in the frequency distribution of calcification stage between the groups.

정상교합 이동의 adenoid에 관한 연구

양규호 大韓小兒齒科學會 1992 大韓小兒齒科學會誌 Vol.19 No.2

Impaired breathing is known to represent a significant contributing to the etiology of dentofacial deformities during childhood growth. Adenoids have long been regarded as one of the chief causes of mouth breathing. The purpose of the present investigation was to evaluate the size of adenoid during childhood growth and present a system which will quantify and status of pediatric patients being considered for adenoidectomy. The author analyzed 9 items in cephalometric roentgenograms of 70 boys and 70 girls taken from the age of 7 to 13. The results were as follow : 1. The mean and standard deviation for the measured items were obtained. 2. The continual growth of nasopharyngeal cavity was more affected by the height than the depth. 3. The size of adenoid was larger in males than in females, especially on R,D-Ad₂ 4. Adenoid percentage was the highest at age 10 in boys and at age 9 in girls. 5. The items showed significant difference between males and females were adenoid percentage, Air(P<0.01). The results suggest that the mean & standard deviation for the measured items could be used to diagnosis malocclusion caused by mouth breathing.

Bionator를 이용한 Ⅱ급 부정교합의 치료에 관한 증례

신강호,양규호 大韓小兒齒科學會 1992 大韓小兒齒科學會誌 Vol.19 No.2

Bionator is an arch-aligning appliance. These appliances can be used to increase the vertical dimension only, or advance the mandible from a Class Ⅱ molar relation to a ClassⅠ only without increasing the vertical, or in combination with either one or both actions also moderately widen the maxillary and mandibular dental archs, especially in the lower anterior area. The authors treated Class Ⅱ malocclusion with Bionator. The results were followed : 1. Severe overjet and large overbite were corrected. 2. Class Ⅱ molar relation was changed by ClassⅠ. 3. Minor crowding of anteriors was treated by adjustment of midline jackscrew and coffin spring. 4. Inclination of upper and lower anterior teeth was corrected by adjustment of labial guide bow and anterior acylic cap. 5. Appliance wearing time was 7-9 months, and retainer wearing for 3 months.

3급 부정교합 아동의 설위치에 관한 연구

양규호 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.1

This study was to investigate the posture of the tongue and hyoid bone in subjects with normal occlusion and Class Ⅲ malocclusion. Thirty eight subjects who had no systemic disease, orthodontic and prosthodontic treatment-20 of normal occlusion, 18 of Class Ⅲ malocclusion-were selected. The posture of the tongue and hyoid bone was measured by lateral cephalogram taken at rest position. Also lateral cephalogram was taken from the same subjects at centric occlusion for craniofacial variables and the posture of the tongue and hyoid bone was compared with craniofacial variables in each group. The results were as follows ; 1. There were significant difference in ANB, APDI, Facial angle, Wits appraisal, ODI, Posterior facial height(S-Go) and IMPA among the craniofacial variables between Class Ⅲ and normal groups. 2. Tongue tip in subjects with Class Ⅲ malocclusion was located at lower and posterior position than that with normal occlusion and the tongue dorsum in subjects with Class Ⅲ malocclusion was located at lower position than that with normal occlusion. Also, tongue length with Class Ⅲ malocclusion was shorter than that with normal occlusion. 3. There was no significant difference for the posture of the hyoid bone between the Class Ⅲ and normal groups. 4. The postures of the tongue and hyoid bone were assoicated with vertical components more than horizontal components of the craniofacial variables.

Modified Twin Blocks에 의한 성장기 아동의 Ⅱ급 부정교합의 치료증례

박재홍,양규호 大韓小兒齒科學會 1994 大韓小兒齒科學會誌 Vol.21 No.2

The Twin Blocks technique was developed by Dr. William Clark of Scotland during the early 1980's. Twin Blocks are an uncomplicated system that incorporates the use of upper and lower bite blocks. These blocks reposition the mandible and redirect occlusal forces to achieve rapid correction of malocclusions. They are also comfortable and the patients wear them full-time-inducing eating time. Occlusal forces transmitted through the dentition provide a constant proprioceptive stimulus to influence the rate of growth and the trabecular structure of the supporting bone. The features of Twin Blocks mean easier and quicker treatment. The occlusal inclined plane is the fundamental functional mechanism of the natural dentition. Twin blocks are bite blocks that effectively modify the occlusal inclined plane to induce favorably directed occlusal forces by causing a functional mandibular displacement. Upper and lower bite blocks interlock at a 45˚ angle and are designed for full-time wear to take advantage of all functional forces applied to the dentition including the forces of mastication. The patients who were treated with modified Twin Blocks, and following results were observed : 1. Large overjet and deep overbite were corrected. 2. ClassⅡ molar relationship was changed into classⅠ. 3. Labial inclination of upper incisors was corrected by adjustment of labial bow of upper bite block. 4. The profiles of two patients were improved by anterior displacement of mandible.

Air Rotor Stripping with Essix Anterior Anchor를 이용한 교정치료

최남기,김숙의,양규호 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.1

Air turbine handpiece를 사용하여 치간 법량질을 삭제하는 Air-rotor stripping 술식은 악궁확장이나 발치 술식에 대한 대체방안으로써 arch length discrepancy를 해결할 수 있는 효과적인 접근법으로 시료된다. Conventional interproximal stripping using abrasive strips is normally limited to the anterior teeth. The strips must be forced between the contact points of the teeth creating patient discomfort and the risk of cutting gingival tissue. Air-rotor stripping (ARS) with Essix anterior anchor enables the clinician to remove a precise amount of interproximal enamel to create space, primarily in the buccal quadrants, for aligning or retracting teeth. In selected cases, ARS can resolve significant differences in ratios of tooth size to arch length, and the technique can become an alternative to extraction or expansion. ARS can create substantially more space than that is usually obtained by conventional interproximal stripping, and it can be done at any time during treatment without discomfort to the patient and without adversely affecting the function of the dentition, interocclusal relations, or tooth form.