다릅나무 종자의 초저온 저장이 발아 특성에 미치는 영향

한심희,김찬수,장석성,이현주 한국산림과학회 2004 한국임학회 정기학술발표논문집 Vol.2004 No.-

아연폐광산(亞鉛廢鑛山) 주변(周邊) 토양(土壤)의 중금속(重金屬) (Cd, Cu, Zn, Pb) 오염(汚染)에 따른 5개(個) 수종(樹種)의 부위별(部位別) 중금속(重金屬) 축적(蓄積)

한심희,현정오,이경준,조덕현,Han, Sim Hee,Hyun, Jung Oh,Lee, Kyung Joon,Cho, Duck Hyun 한국산림과학회 1998 한국산림과학회지 Vol.87 No.3

본 연구는 폐광지 주변에서의 토양내 중금속(Cd, Cu, Zn, Pb) 오염 정도와 토양내 중금속 농도와 수목내 중금속 축적 농도와의 관계를 규명하여, 오염지역의 정화에 수목을 이용할 수 있는지를 확인하는 것을 목적으로 수행하였다. 경기도 화성군에 위치한 삼보광산과 광명시에 있는 가학광산에서 1997년 4월부터 9월에 걸쳐 토양 시료와 주변의 난티잎개암나무, 리기다소나무, 현사시나무, 진달래, 아까시나무를 채취하여 중금속 농도를 측정하였다. 두 광산지역의 광구에서 500m 이내 주변 토양은 중금속에 의한 오염 정도가 28~143ppm으로써 심각하지 않았으나, Zn(143ppm), Pb(97ppm)과 같은 중금속 농도는 독성을 나타낼 수 있는 수준이었다. 토양내 중금속의 농도는 Zn>Pb>Cu>Cd의 순이었으며, 광구의 중심에서 1.5km 이상 멀어질수록 증금속 농도는 10ppm 이내로 감소하였다. 각 수종별 중금속의 농도는 Pb를 제외하고, 현사시나무에서 가장 높았으며, 특히 잎에 고농도로 축적되어 있었고, Zn의 경우 91ppm이 검출되었다. 난티잎개암나무의 뿌리에서는 Cu와 Pb의 높은 농도로 검출되었다. 토양의 중금속과 수목 조직내 중금속 농도는 매우 높은 정의 상관을 보여주었으며, 리기다소나무에서 가장 높은 상관계수를 나타냈다. 토양에 대한 수목 조직의 중금속 농도비(Concentration Factors : CF)는 Zn이 가장 높은 값을 보였고 Pb이 가장 낮은 값을 보여주었다. 현사시나무는 CF값이 가장 높아서 중금속을 가장 많이 축적하고 있었다. 특히 토양의 중금속 농도에 대한 식물체의 농도비가 가장 높은 현사시나무는 중금속의 흡수 능력이 뛰어나 중금속 오염지에서 정화식물로 이용할 수 있으며, 리기다소나무는 토양의 오염 정도를 가장 잘 반영하는 수종으로 판단된다. This study was conducted to evaluate heavy metal concentrations(Cd, Cu, Z, and Pb) in the soil of two zinc mines and to correlate heavy metal contents between the soils and trees growing near the mines. Soils and leaves, stems, and roots of five tree species(Corylus heterophylla, Pinus rigida. Populus alba${\times}$glandulosa, Rhododendron mucronulatum, and Robinia pseudoacacia) were collected from Sambo Zinc Mine located in Hwasung and Gahak Zinc Mine in Kwangmyung city in Kyonggido. Soils near two zinc mines were not seriously contaminated by heavy metals, but Zn and Pb concentrations were at toxic level. The heavy metal concentration in soils decreased in the order of Zn, Pb, Cu, and Cd, and decreased with increasing distance from zinc mining sites. Among the five tree species, Populus alba ${\times}$ glandulosa showed the highest heavy metal concentrations in the tissue except for Pb. Particulars, leaves of the species contained the high concentrations of heavy metals to reach the maximum of 91ppm Zn. The roots of Corylus heterophylla contained high concentrations of Cu and Pb. The order of heavy metal concentrations in the tree species was Zn, Cu, Pb and Cd. The concentration of heavy metals in the tree tissues showed a positive correlation with that in soil in which trees are growing. The ratio of heavy metal concentration of trees to that of soils(concentration factor : CF) was highest in Zn and lowest in Pb. Populus alba${\times}$glandulosa had the highest CF value among the five tree species. It was concluded that Populus alba${\times}$glandulosa, based on the high metal uptake ability, could be used for decontaminating of heavy metals from contaminated soils, and Pinus rigida could be used to reflect the level of contamination in soils.

초저온 저장이 다릅나무 종자의 발아와 유묘의 생장 특성에 미치는 영향

한심희,김찬수,장석성,이현주 한국자원식물학회 2004 한국자원식물학회지 Vol.17 No.2

This study was conducted to investigate effects of cryopreservation by vitrification on the seed germination rate and growth and physiological properties of seedlings of Maackia amurensis. Cryopreservation significantly decreased the germination rate of seeds of M.amurensis, but the reduction of germination rate was mitigated by the treatment of cryoprotectant (plant vitrification solution, PVS2) before plugging into liquid nitrogen and fast thawing rate after cryopreservation. Long-term PVS2 exposure decreased seed germination rate, whereas cryopreservation time didn’t have influence on seed germination rate. In addition, growth and physiological properties of seedlings were not affected by PVS2 exposing time and cryopreservation time. Therefore cryopreservation could be widely used as a technique of long-term ex situ conservation without any damage and deterioration of cells or tissues of the forest seeds. However, in order to increase the effect of cryopreservation, we have to develope the lower toxic cryoprotectant and suitable techniques to the structural or chemical properties of a variety of seeds.

기획시리즈 - 사라져가는 우리나라특산식물 '산개나리'

한심희,Han, Sim-Hui 한국조경수협회 2011 造景樹 Vol.121 No.-

산성우에 의한 토양산성화에 대한 4개 수종의 완충능력과 수관으로부터 양료 용탈 변이

한심희,이경준 ( Sim Hee Han,Kyung Joon Lee ) 한국산림과학회 1997 한국산림과학회지 Vol.86 No.3

The objective of this study was to compare acid-neutralizing and buffering capacity of four tree species against soil acidification by acid rain. About 30-year-old forests composed of Pinus rigida, Alnus hirsuta, Quercus mongolica, and Liriodendron tulipifera in a provincial experimental forest located 151an east from Banwol Industrial Complex in Kyonggido were used in this study. Incident precipitation, throughfall and stemflow, and soil samples were collected from May to September, 1996 to analyze their pH and canon concentrations. Internal leaf pH, external acid neutralizing capacity(ENC), and buffering capacity index(BCI) of leaves were also determined. The incident precipitation showed an average pH of 4.56, with the percentage of acid rain incidents being 74%. The average soil pH of the study area was 4.15. The pH of throughfall and stemflow in all four species was higher than that of precipitation except that of the stemflow of Pinus rigida which showeed a pH of 3.73. The throughfall of Liriodendron tulipifera showed the highest pH of 5.38. The pH of throughfall and stemflow showed a positive correlation and no correlation, respectively, with precipitations. The most abundant cation in precipitation was Ca. The canon concentraions in throughfall and stemflow decreased in the following order of K, Na, Ca, and Mg. Cation concentrations in stemflow were highest in Lirioendron tulipifera and lowest in Pines rigida. Nutrient leaching from above ground increased with decreasing pH of precipitation. The pH of stemflow showed a positive correlation with ENC and BCI. The highest values in ENC, BCI, soil pH, and soil canon concentrations were observed in Liriodendron tulipifera, while the lowest values were obtained in Pinus rigida, It was concluded that Liriodendron tulipifera had highest neutralizing capacity against acid rain, while Pines rigida had the lowest capacity and even promoted acidification of soil.

Effects of Cone Number and Thinning on Nutrient Content in Needles of Korean Pine (Pinus koraiensis)

한심희,김인식,김두현,김태수,장경환,김태동 한국산림과학회 2008 한국산림과학회지 Vol.97 No.5

We investigated on the distribution of nutrient contents (N, P and K) in current and one-year old needles of Korean pine, Pinus koraiensis, to understand the physiological effects of alternate bearing. One experimental set was designed with three types of cone number (one, three and five) under natural state and cone number of another one was controlled by thinning. Test samples were separated to collect into three fractions; current needles on the shoot and one-year old needles above and under the cone. On the branch without cone, N, P and K concentrations in needles on the shoot were higher than those in needles of one-year old branch. At natural state, needle P concentration was the lowest in needles on the five-cone branch in August. Macronutrient concentrations were the lowest in needles above the cone, which was remarkably observed in needle K concentration. Under cone thinning, N, P and K concentrations were the highest in needles on one-cone branch and the lowest in needles on five-cone branch. Needle nutrient concentrations were the lowest in needles above the cone, which was remarkably observed in the needle on five-cone branch. In conclusion, the current needles of the shoot and cones are a strong sink of nutrient like N, P and K, and the increase of cone number influences nutrient removal from needles.

환경오염지에서 생육하는 사방오리나무의 색소함량 및 항산화능력의 계절변화

한심희,이재천,오창영,김종갑,김판기 한국농림기상학회 2006 한국농림기상학회지 Vol.8 No.2

아연폐광산 주변 토양의 중금속 ( Cd , Cu , Zn , Pb ) 오염에 따른 5개 수종의 부위별 중금속 축적

한심희,현정오,이경준,조덕현 ( Sim Hee Han,Jung Oh Hyun,Kyung Joon Lee,Duck Hyun Cho ) 한국산림과학회 1998 한국산림과학회지 Vol.87 No.3

This study was conducted to evaluate heavy metal concentrations(Cd, Cu, Z, and Pb) in the soil of two zinc mines and to correlate heavy metal contents between the soils and trees growing near the mines. Soils and leaves, stems, and roots of five tree species(Corylus heterophylla, Pinus rigida. Papulus alba × glandulosa, Rhododendron mucronulatum, and Rabinia pseudoacacia) were collected from Sambo Zinc Mine located in Hwasung and Gahak Zinc Mine in Kwangmyung city in Kyonggido. Soils near two zinc mines were not seriously contaminated by heavy metals, but Zn and Pb concentrations were at toxic level. The heavy metal concentration in soils decreased in the order of Zn, Pb, Cu, and Cd, and decreased with increasing distance from zinc mining sites. Among the five tree species, Populus alba × glandulasa showed the highest heavy metal concentrations in the tissue except for Pb. Particulars, leaves of the species contained the high concentrations of heavy metals to reach the maximum of 91ppm Zn. The roots of Corydus heterophylla contained high concentrations of Cu and Pb. The order of heavy metal concentrations in the tree species was Zn, Cu, Pb and Cd. The concentration of heavy metals in the tree tissues showed a positive correlation with that in soil in which trees are growing. The ratio of heavy metal concentration of trees to that of soils(concentration factor : CF) was highest in Zn and lowest in Pb. Populus alba×glandulosa had the highest CF value among the five tree species. It was concluded that Populus alba×glandulosa, based on the high metal uptake ability, could be used for decontaminating of heavy metals from contaminated soils, and Pinus rigida could be used to reflect the level of contamination in soils.

폐석지내 강광 스트레스에 대한 사방오리나무 잎의 항산화 보호

한심희,이재천,이위영,박영기,오창영,김종갑 한국임학회 2006 한국임학회 정기학술발표논문집 Vol.2006 No.-

Adaption of Betula schmidtii Seeding in Coal-mine Field with Different Sewage Sludge Treatment Methods

한심희,이재천,오창영,김판기 한국생태학회 2005 Journal of Ecology and Environment Vol.28 No.4

We tested the field adaptation of Betula schmidtii on the abandoned coal-mine soil with sludge amendment methods for romoting physiological activity of B. schmidtii seedlings under several environmental stress. Sewage sludges were amended to coal-mine soil with B. schmidtii seedlings which grown in the mixtureof artificial soil and composted sludge soil before transplanting (before-fertilized treatment, BF) and fertilized with composted sludge after transplanting (after-fertilized treatment, AF). The percent of establishment of seedlings for AF (80.7%) was lower than that for BF (92.7%). Nitrate reductase activity and photosynthetic pigment content were higher in AF than in BF, but malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were lower in AF than in BF. These results represent that after-fertilized seedlings increase resistance against physiological stress at field condition using nitrogen source of composted sludge. On the contrary, before-fertilized seedlings were susceptible to environmental stress on abandoned coal-mine soil by exhausting of nitrogen source from composted sludge.