임목 : 임목의 양적유전과 집단유전

현정오 한국육종학회 1988 한국육종학회 연구집 Vol.1988 No.-

CEPA 처리가 옻나무의 칠액분비 및 수피생리에 미치는 영향과 옻나무 칠액 채취법 개량에 관한 연구

현정오 서울대학교 농업개발연구소 1999 농업생명과학연구 Vol.3 No.-

This study was conducted to investigate the effects of CEPA(2-chloroethyl phosphonic acie) on bark physiology and anatomy of lactree(Rhus verniciflua Stokes). Sample trees of similar size and growth rate were selected from 7-year-old lactree plantation located in Hyengsun-kun, Kangwon-do. Lanolin pastes containing 0.1, 1, or 10% CEPA were put into the bark-removed hole made by corer(Φ1cm) on the main stem at 1.2m above the ground on June 16, 1995. Five weeks after application of CEPA, bark thickness was markedly increased as a result of the increase in the amount of phloem and intercellular spaces, and correlated with the increased production of urushiol. By the application of 10% CEPA, bark thickness was increased approximately 2.5 times, and the urushiol content within bark was increased 2.8 times compared to that of untreated trees because CEPA stimulated the accumulation of urushiol within bark. Treatment of 10% CEPA also increased the size and the total number of secretory canals, and induced an increase in ray width. The phloem parenchyma cells of CEPA-treated trees were well-developed and closely packed with little intercellular space. In order to improve 'Salso' method(conventional tapping method) which was very inefficient in the aspects of collection time and labor, this study was carried out to investigate the effect of the application date and the distance from treatment point in the lactree(Rhus verniciflua) treated with 10% CEPA and to decide the possibility of application of the technique in the field. Bark thickness was significantly increased to the part 40cm above and below the treatment point, but urushiol content was increased to the part 20cm above and 10cm below the zone treated with 10% CEPA. The urushiol content of the bark was highest at 5cm above the treated zone and decreased in the order of 10, 20, and 40cm. And the urushiol contents of the bark of the upper part were higher than that of the low part. The effects of CEPA on bark thickness were similar to urushiol contents. Urushiol production of lactree is highly dependent on climatic conditions and particularly on the precipitation, and duration of sunshine. Ten percent of CEPA-lanolin pastes which was treated on June 16 affected bark anatomy and urushiol contents, while the treatment on August 24 did not affect. In the Rhus verniciflua treated with 10% CEPA, the urushiol contents was initially increased from 7 days after treatment, continued for the 4 weeks, and then slight decrease occurred at 5 weeks after the treatment. We measured a total sap yield by Salso method in lactree treated with 10% CEPA. By applying 10% CEPA, the sap yield was increased 3-4 times compared to that of untreated trees in the first tapping, But the relative ratio was gradually decreased from the second tapping to sixth, and after seventh tapping, the untreated trees secreted more sap than the treated trees. We discussed about the causes.

RAPD 표지자를 이용한 국내 자생 황칠나무 천연집단의 유전변이 및 보전전략

현정오 서울대학교 농업개발연구소 2000 농업생명과학연구 Vol.4 No.-

Dendropanax morbifera Lev. has been traditionally considered as an important species for golden lacquer production in Korea. But now the geographical disturibution of this species is restricted to a few islands and the population size has been decreased. The pattern and partitioning of genetic variation is important baseline knowledge for ex. situ. Conservation and tree breeding. To establish conservation strategy, we examined genomic DNA polymorphisms via the analysis of RAPD in the natural populations of D. morbifera from southern Korea. A total of 120 trees derived from 6 populations were analysed using 6 arbitrary 10-mer primers. Genetic diversity and structure of the natural populations of D. morbifera was estimated by AMOVA (analysis of molecular variance) procedure. The average value of percent of polymorphic markers was 72.2%. Most variation(80.5%) existed among individuals within population. Genetic differentiation among populations (θst ) was 0.196. On the nested analysis, the genetic variability among individuals accounted for 76.3%, among population for 12.6% and among region for 11.1%. When the populations were grouped into two regions (i.e., southern coast, and Jeju island region), 15.6% of the total genetic variation was explained as regional differences. Using UPGMA cluster analysis based on Nei's unbiased genetic distance, the populations were assembled according to their regions. The heterogenetiy of genetic variance between populations was investigated by pairwise Bartlett's test, which revealed that all of the pair of populations were significantly heterogeneous.

산림생태계의 생물다양성 보존 및 서식지 복원을 위한 연구 : 구상나무와 분비나무 천연집단의 유전변이 분석

현정오 서울대학교 농업생명과학대학 농업개발연구소 1997 농업생명과학연구 Vol.1 No.-

산림생태계의 생물다양성 보존 및 서식지 복원을 위한 연구 : 구상나무림을 중심으로

현정오 서울대학교 농업생명과학대학 농업개발연구소 1998 농업생명과학연구 Vol.2 No.-

Abies koreana Wilson is an endemic conifer species in Koeaa. Its natural distribution is restricted to the subalpine areas of the southern high rising mountains such as Mt. Halla, Mt. Jiri and Mt. Dukyu. Recently, the decline in growth of A. koreana became obvious and it appears to be a serious problem for maintaining the populations. The decline in growth of the species is not limited to the one area but it is rather rangewide. Besides the problem of declining in growth, the poor regeneration of the species was found to be an even more serious problem for the long term survival of the species. This research was conducted to investigate the environmental characteristics and the structure and growth of A. koreana natural stands, to investigate the genetic structure of the species and to develope a technique for restoring the damaged A. koreana stands. Genetic structure in DNA level revealed that the 18.45% of the total genetic variation was found among populations indicating a significant differentiation among populations. The Mt. Halla population found to be genetically very different from other inland populations. This means that it is important to use the right source of plant materials with plenty of genetic variation when restore the damaged stands of the species. It was found that a serious decline in growth of the A. koreana trees occurred in the year of drought or following the drought year. The various species of mycorrhizae were found in the stands of Mt. Jiri and Mt. Halla, but no common species were found in both area.

산칠량이 (産漆量) 많은 옻나무개체의 선발에 관한 연구

현정오,김만조,이세표 ( Jung Oh Hyun,Mahn Jo Kim,Shae Pyo Lee ) 한국산림과학회 1993 한국산림과학회지 Vol.82 No.2

This study was conducted to investigate characteristics related to lacquer yield and to evaluate possibility of indirect selection of Rhus verniciflua Stokes. Twenty-four lacquer trees were randomly selected for measuring growth, urushiol content and bark thickness. Urushiol content showed a significant positive correlation with outer bark thickness and secretory canal density at 1% level, and with inner bark thickness at 5% level. Bark thickness and secretory canal density could be used as indicators for high urushiol content within bark and thus for high lacquer yield. Mass selection for high lacquer yield was made based on urushiol content within bark. Sixteen individuals with the highest urushiol content value were selected for breeding. As expected, the selected trees had thicker bark. higher density of secretory canal, better developed secretory canals compare to the non-selected lacquer trees.

육종 (育種) · 조직배양 분야 : GA 4/7 처리에 의한 소나무의 화기형성 촉진에 관한 연구

현정오,홍경낙 한국임학회 1989 한국임학회 학술발표논문집 Vol.1989 No.-

캐나다 British Columbia 대학교 식물원 소개

현정오 서울大學校 農業生命科學大學 附屬樹木園 1996 서울大學校 樹木園 硏究報告 Vol.- No.16

지난 여름 8월 5일부터 9일까지 IUFRO의 한분과인 삼림집단유전 및 삼림생태유전학회가 캐나다 뱅쿠버에 있는 UBC(The University of British Columbia)에서 개최되어 참석하였다가 UBC Botanical Garden을 방문할 기회를 가질 수 있었다. 이 식물원은 약 26정보의 규모로 큰 편은 아니지만 Douglas Fir와 Western Hemlock등 키가 큰 자연 숲과 조화시켜 조성한 것이 돋보이는 매우 아름다운 식물원이다. 지금까지 세계 여러 식물원이 국내 관련잡지에 소개된 바 있으나 UBC식물원은 소개된 적이 없어 이번에 UBC식물원 소개책자를 참고해서 상세하게 소개하고자 한다.

Electroporation 방법에 의한 현사시나무의 형질전환

현정오,손석규 한국임학회 1992 한국임학회 정기학술발표논문집 Vol.1992 No.-

國內 常緣性 참나무類의 遺傳的 構造 및 多樣性과 붉가시나무 集團의 保全戰略

현정오 서울대학교 농업개발연구소 2001 농업생명과학연구 Vol.5 No.-

This study was conducted to estimate the genetic variation and the phylogenetic relationship of the Quercus species of the subgenus Cyclobalanopsis and to examine a spatial genetic structure of Q. acuta population in Korea. When genetic variation of Q. acuta, Q. glauca, and Q. salicina were estimated by AMOVA (analysis of Molecular variance) procedure, most of the genetic variation reside within populations. The three species of the subgenus Cyclobalanopsis, the genetic diversity was relatively low and the genetic distance between populations was very small, but a slight differentiation between the species appeared to be exist. The analysis of phylogenetic relationship revealed that the three species formed different clade in the UPGMA and NJT tree. This indicates that sexual isolation between the three species was developed, and thus hybridization among them was not occurring frequently. In the clonal diversity and fine-scale genetic structure in Mt. Baekun population of Q. acuta, of the 120 trees, only two individuals were identified as a clone. Most individual had a unique genotype. This indicates that trees of the Mt. Baekun population were regenerated by stem sprouts rather than root sprouts after disturbances. Moran's I-statistics revealed that most of the genetic similarity was shared by individuals separated by less than 17-21m in distance, and there were a trend along the slope. This indicates that they themselves are distributed in substantially structured manner resulting from isolating by distance. Donnaeko population in Jejudo and Mt. Baekun population in Wando are thought be the most important populations for in situ conservation because they are larger in size and more diverse than others. If it is necessary to conserve one more population, Mt. Chumchal population in Chindo must be added because they are most heterogeneous. Since genetic distances both among connected populations and among isolated populations were very small, for conserving genetic variability of Q. acuta, maintaining of the natural populations would be more effective than transplanting of individuals originated from other populations. For ex situ conservation and restoration of Q. acuta, it is recommended that a total of 500 individuals should be collected at least 40m apart each other.