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최홍란,고영종,임원봉,김지선,김인애,권혁일,김옥수,김병국,김선미,윤숙자,강병철,임회순,김미숙,김옥준 대한구강악안면병리학회 2010 대한구강악안면병리학회지 Vol.34 No.2
Candida albicans and their associated Candida species are opportunistic pathogens which exists as normal flora in the oral cavities of healthy individuals. In response to physiological changes in the host, these yeasts can become pathogenic, resulting in oral candidiasis. The rapid detection and identification of Candida species in clinical laboratories are extremely important for the management of patients with hematogenous candidiasis. The presently available culture and biochemical methods for detection and species identification of Candida are time-consuming and lack the required sensitivity and specificity. In this study, we have established a seminested PCR (snPCR) using universal and species-specific primers for detection of Candida species in saliva. The universal outer primers amplified the 3end of 5.8S ribosomal DNA (rDNA) and the 5end of 28S rDNA, including the internally transcribed spacer 2 (ITS2), generating 350- to 410-bp fragments from the four commonly encountered Candida spp., viz., C. albicans, C. tropicalis, C. glabrata, and C. parapsilosis. The saliva from 331 healthy and, over 50 years of aged people lived in Dong-gu, Gwangu city, was collected. Total DNA were extracted by Hoffman-Winston yeast total DNA prep. method and performed the snPCR. Results appeared to be negative on 292 people (88.2%), however, 26 people (7.9%) were positive Candida albicans, 6 people (1.8%) were positive Candida glabrata, 5 people (1.5%) were positive Candida tropicalis, and only 2 person (0.6%) were positive Candida parapsilosis. These result showed that detection and identification of Candida species could be established by saliva analysis, so that snPCR on saliva is useful method of diagnosis of clinical fields.
백서의 피하에 이식한 치수에서의 mineralization에 대한 형태학적 연구
최홍란 전남대학교 치과대학 1989 전남치대논문집 Vol.1 No.2
The dental pulp is the soft connetive tissue that chiefly composed of odontoblasts, fibroblasts, undifferentiated mesenchymal cells, and macrophages. The odontoblast is important in the formation of dentin, and its function is similar to that of the osteoblast in intramembranous bone formation. It was established that odontoblasts strongly resemble to osteoblasts after calcification of dentin and, when cultured in vitro, they are similar to each other morphologically and cytochemically. Bone and dentin are closely related in both orgnaic and inorganic composition. The present study was undertaken to observe the morphologic changes of transplanted pulp cells in rats. The results were as follows : 1. Light microscopically, at the early stage(1-3 days) of transplantation, hyalinized tissues and calcospherites around of odontoblasts and fibroblasts of the transplanted tissue were observed. Osteoid tissues and spongy bone surrounded by osteoblasts were observed at the middle stage(5-11days). With the time lapsed, mineralization of the bones were advanced. More advanced mineralization in bone tissue was observed but osteoid tissues were not observed at the late stage(2-4 weeks). 2. Electron microsopically, at the early stage of transplantation, the cells having ecentric nucleus with abundant cytoplasm, well developed rough endoplasmic reticulum and round shaped of mitochondria and resembled osteoblasts. A few of collagen fibers were observed. At the middle stage, osteoid and osteblasts were observed. Osteoid tissures were consist of irregularaly arranged of collagen fibers and osteoblasts disclosed well developed rough endoplasmic reticulum with cisternal dilatations. Calcifying globules and calcium deposits were observed in osteoid tissue. From the above results, it is suggested that pulps transplanted subcutaneously, were observed formed the bone tissues and as bone formations and maturations are various accoring to the changes of environment and durations. It is thought that odontoblasts, fibroblasts and undifferentiated mesenchymal cells are invloved in bone formation.