중합효소연쇄반응을 이용한 Y염색체의 검색

최영민,신창재 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.3

저자들은 임상적으로 흔히 성별 판정에 이용될 수 있는 양수천자세포 10예, 융모막 생검조직 6예, 협측도말세포 6예를 대상으로 중합효소연쇄반응을 이용하여 Y 염색체 특이 염기서열(DYZ1)을 검색한 결과, 조직 및 세포 배양에 의한 핵형분석과 일치되는 결과들을 얻었다. 임상적으로 Y 염색체 검색을 필요로 하는 경우 중합효소 연쇄반응을 이용하면 소량의 검체를 사용하여 신속하고 정확한 결과를 얻을 수 있을 것으로 사료된다. We demonstrated th use of in vitro amplification of Y chromosome-specific repeated DNA sequences for rapid determination of fatal sex with amniotic fluid, chorionic villus and buccal smear samples. The oligonucleotide primers were synthesized for a 149 bp DNA segment within 3.4kb(DYZ1 family) Y chromosome-specific sequendes. The amplified DNA segments could be observed as bright bands in the expected size range of 8% polyacrylamide gel after 30 amplification cycles in samples of male DNA. No PCR products were observed in samples of female DNA under the same conditions. Polyerase chain reaction can be used as a rapid and useful method for the prenatal sex determination.

증상이 있는 간의 염증성 근육 섬유 모세포종 1예

최영민,김상범,박선후,최동욱 한국간담췌외과학회 2004 한국간담췌외과학회 학술대회지 Vol.2004 No.5

도금욕 조성이 용융아연 합금화도금층의 가공성에 미치는 영향

최영민,장세기 대한금속재료학회(대한금속학회) 1993 대한금속·재료학회지 Vol.31 No.10

Hot dip galvannealed coatings on steel sheet were evaluated with respect to minor additions of aluminum, lead or antimony in the zinc bath. The decrease of aluminum content in the bath increased alloying rate of the coating and promoted the growth of Gamma phase(Fe₃Zn_(10)) to deteriorate powdering resistance of the galvannealed coating. The variation of lead content in the bath did not noticeably change galvannealing behavior of the coating, but powdering resistance was deteriorated with lead addition. On the other hand, alloying rate of the coating increased with antimony addition, which inversely affected powdering resistance of the coating. The use of antimony tended to change a distribution of aluminum in the coating which influenced the zinc-iron allaying reaction at the interface of the coating and steel sheet.

주제공원 활성화를 위한 방문객 만족수준에 관한 연구

최영민 순천향대학교 사회과학연구소 1999 순천향 사회과학연구 Vol.4 No.2

The main aim of this paper examines the factors influencing revisit to theme park. From the previous studies, authors drew 29 attributes of theme parks attractions to identify the relation between the level of customers' satisfaction and their evaluation of theme park attractiveness. The data was collected from survey questionnaires on five theme parks in the suburbs of Seoul. The empirical test discovered that some attributes had significant differences between the ex ante expectation level and ex post satisfaction level, and that there were also varying differences in satisfaction level according to the general charac- teristics of customers. This study also showed that adventurous atmosphere, staffs' friendliness, rest space, stress res- olution, operation hours were instrumental factors in determining customers' re-visitation of theme park

근치적자궁적출술후 재발성 자궁경부암의 치료 및 예후에 관한 연구

최영민,강순범,이효표,박만철,신면우,안기범 대한산부인과학회 1990 Obstetrics & Gynecology Science Vol.33 No.5

1979년 1월부터 1987년 5월까지 서울대학교병원 산부인과에서 침윤성 자궁경부암으로 진단되어 근치적 자궁적출을 받은 환자 중 추적조사에서 발견된 재발환자는 78명이었다. 78예의 재발성 자궁경부암을 임상적으로 분석 검토하여 다음과 같은 결과를 얻었다. 1. 연령별 분포는 40대에서 전체의 43.6%로 가장 빈도가 높았다. 2. 세포형태는 편평상피암이 전체의 92.3%로 가장 많았고, 선암 5.1%, 선상피암이 2.6%였다. 3. 원래의 임상기별 분포는 Ib기가 39.7%로 가장 많았고, IIa기 38.5%, IIb기가 21.8%였다. 4. 재발부위분포는 중앙골반재발이 16.7%, 골반측변재발이 35.9%, 원격전이 47.7%였다. 5. 원격전이가 가장 빈번히 일어나는 장기는 폐였다. 6. 근치수술 후 보조적 방사선 치료에 의해 재발부위 분포의 차이가 있었다. 7. 수술후 재발까지의 기간은 1년이내에 53.8%가 재발했고 2년이내에 80.7%가 재발하였다. 8. 임상기가 진행된 암일수록 재발까지의 기간이 짧았다. 9. 중앙골반재발이 골반측벽 재발이나 원격전이보다 유의하게 일찍 일어났다. 10. 39예(50%)가 증상없이 정기추적검사에서 재발이 발견되었다. 11. 재발환자의 누적 생존율은 재발 후 6개월 67.6%, 1년에 40.5%, 2년에 21.6%이었으며 5년 생존율은 10.8%였다. 12. 원격전이가 중앙골반재발이나 골반측벽재발보다 누적 생존율의 낮았다. 13. 재발 후 적절한 치료를 받은 군이 비치료군보다 누적 생존율이 높았다. 14. 수술 요법만을 받은 군이 수술 후 보조적 방사선 치료를 받은 군보다 누적생존율이 높았다. Seventy-eight patients who underwent radical hysterectomy for invasive cervical cancer at the SNUH from January, 1979 to May, 1987 were diagnosed as recurrent cervical cancer. The retrospective analysis was made on 78 patients in terms of clinical features and prognosis. The results were as follows: 1. The highest incidence occurred in patients aged 40-49. 2. Histopathologic types were distributed as follows: squamous cell carcinoma was 72 cases (92.3 %), adenocarcinoma was 4 cases (5.1 %), and adenosquamous carcinoma was 2 cases (2.6 %). 3. Distribution of initial clinical stage was as follows: stage Ib, 31 cases (39.7 %); stage IIa, 30 cases (38.5 %); stage IIb, 17 cases (21.8 %). 4. Site of recurrence was as follow: central pelvic recurrence, 16.7 %; pelvic sidewall recurrence, 35.9 %; and distant recurrence, 47.4 %. 5. The most common site of distant recurrence was lung. 6. Postoperative radiation therapy influenced the site of recurrence. 7. Fifty-four percent of recurrences were observed within the first 12 months after surgery, 80.7 % within 2 years and 91.2 % within 3 years. 8. The more advanced cancer showed the shorter time to recurrence. 9. Central recurrences occurred significantly earlier than pelvic sidewall and distant recurrences. 10 Thirty-nine patients (50 %) had no symptoms and were diagnosed as recurrences on routine follow up examination. 11. Cumulative survival rates after recurrence were as follows: 6-month survival, 67.6 %; 1year survival, 40.5 %; 2 year survival, 21.6 %; 5 year survival rate was 10.8 %. 12. Distant recurrence showed lower cumulative survival than central pelvic recurrence or pelvic sidewall recurrence. 13. The patients treated after recurrence showed higher cumulative survival rate than untreated patients. 14. The patients treated with radical surgery only showed higher cumulative survival rate than the patients treated with radical surgery and adjuvant radiation-therapy.

17β-estradiol이 인간의 자궁근종으로부터 배양된 조직에서 인슐린유사 성장인자 결합단백질들의 생성에 미치는 영향

최영민,이진용,장윤석,문신용,김정구,신창재,김석현 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.1

자궁근종의 중요한 성장인자로 알려진 에스트로겐의 일부작용이 IGFBP들의 생성변화를 초래함으로써 이루어지는가를 규명하고자 자궁근종조직과 정상 자궁근육조직에서 유출된 세포질분획 및 17B-estradiol이 배양액내 첨가되어 6일간 배양된 자궁근종조직과 정상자궁근육조직의 배양액네에서 Western ligand blotting과 면역침전법(immunoprecipitation)에 의해서 측정될 수 있는 IGFBP들의 분포양상 및 그 강도를 비교분석하여 다음과 같은 결과를 얻었다. 1. 자궁근종조직의 세포질분획과 배양액내에서 IGFBP-2, IGFBP-3, IGFBP-4, 26kDa IGFBP가 검출될 수 있었으나 이중 IGFBP-4만이 모든 예에서 검출되었다. 2. 자궁근종조직의 세포질분획내 IGFBP-4강도는 인접 정상자궁근육조직에서의 것과 차이가 없었다. 3. 세포질분획과 배양액에서 검출될 수 있는 각 IGFBP의 빈도는 자궁근종조직과 인접 정상자궁근육조직사이에 차이가 없었다. 4. 17B-estradiol에 노출되었던 자궁근종조직에서 IGFBP-4 강도가 약제에 노출되지 않았던 조직에서의 것보다 유의하게 높았으나, 인접 정상자궁근육조직에서는 이러한 현상이 관찰되지 않았다. 이상의 결과로 보아 자궁근종의 중요한 성장인자로 생각되고 있는 17B-estradiol의 작용이 IGFBP-4에 의해서 중개될 수 있으며 17B-estradiol의 IGFBP-4 생산에 있어서 그 민감도가 자궁근종조직에서 인접 정상자궁근육조직보다 크다고 사료되었다. Uterine leiomyomata are the most common pelvic tumor in women but their etiology is unknown. Estradiol is believed to a stimulus for their growth, acting via the insulin-like growth factors(IGFs), potent mitogenic peptides. IGF-binding proteins(IGFBPs) may modulate IGF actions at their taret cells and directly affect the function of these cells. The purpose of this study is to compare IGFBP profiles in uterine leiomyoma and adjacent normal myometrium and to evaluate the effect of 17B-estradiol on the production of IGFBPs in these explant cultures. IGFBP-2, IGFBP-3 and 26kDa IGFBP were detected variably in tissue cytosols from uterine leiomyoma and adjacent normal myometrium and in conditioned media of explant cultures by western lignad blotting and immunoprecipitation, but IGFBP-4 were consistenly present. There were no differences in the relative intensity for IGFBP-4 and the frequency of each IGFBP between leiomyoma and adjacent normal myometrium. For each patient the complement of IGFBPs was similiar in tissue cytosols and conditioned media of explants. Treatment of leiomyoma explants with 17B-extradiol resulted in significant increase in the intensity of IGFBP-4 in conditioned media when compared to the control condition whereas this treatment did not affect IGFBP-4 production in normal myometrial explants. These data support that IGFBP-4 may be a mediator of 17B-estradiol action in these tissues and that there may be an enhanced sensitivity toward IGFBP-4 production in leiomyoma compared to adjacent normal myometrium.

전라남도 부국, 성산, 영광 폐광산의 태양광 발전 잠재성 평가

최영민,최요순,서장원,박형동,장미향,고와라 한국자원공학회 2013 한국자원공학회지 Vol.50 No.6

This paper assessed photovoltaic potentials at the Buguk, Sungsan and Younggwang abandoned mines in Jeollanam-do, Korea. To decide suitable areas for installing photovoltaic power (PV) systems, digital elevation models were used for analyzing terrain and shading effects. The estimated power productions and economic effects were analyzed using the solar radiation map and RETScreen software. The Buguk abandoned mine has the highest photovoltaic potential where the estimated power production is about 436 MWh/year, and equity payback is 9.1 years. Annual solar radiations at the Sungsan and Younggwang abandoned mines are almost same, however the photovoltaic potential at the Sungsan mine is higher than the one at the Younggwang mine since the Sungsan mine has larger area suitable for installing PV systems. 본 연구에서는 전라남도 부국, 성산, 영광 폐광산에 대한 태양광 발전 잠재성 평가를 수행하였다. 태양광 발전 시스템 설치가 적합한 부지의 면적을 결정하기 위하여 수치지형모델을 이용한 지형분석과 그림자 분석을 수행하였다. 또한, 전라남도 일사량 자료와 RETScreen 소프트웨어를 이용하여 태양광 발전 시스템으로부터 기대할 수 있는 전력 생산량과 경제적 효과를 분석하였다. 그 결과 부국 폐광산이 연간 약 436 MWh의 전력을 생산할 수 있으며, 자기자본회수 기간이 9.1년으로서 가장 높은 잠재성을 보이는 것으로 분석되었다. 성산 폐광산과 영광 폐광산은 일사량 조건은 비슷하지만, 태양광 발전 시스템 설치가 용이한 면적의 크기가 달라서 성산폐광산이 상대적으로 더 큰 태양광 발전 잠재성을 가지는 것으로 분석되었다.

관광기획 환경변화에 관한 연구 -통일독일사례를 중심으로-

최영민 순천향대학교 사회과학연구소 2000 순천향 사회과학연구 Vol.5 No.2

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두 사회구조의 통합을 위한 관광기획에 관한 연구-구 동. 서독 관광통합 기획을 사례로

최영민 순천향대학교 사회과학연구소 2000 순천향 사회과학연구 Vol.6 No.1

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한국인 스와이어증후군 환자 및 가족구성원의 SRY유전자변이의 양상

최영민,이진용,채희동 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.7

Individuals affected with Swyer syndrome are phenotypic females with 46, XY karyotype, sexual infantilism, mullerina derivatives, and bilateral streak gonads that may undergo neoplastic transformation. The pathogenesis of this syndrome is uncertain, but may be related to a defect in the regulation or expression of the testicular determining factor which is believed to be located on the short arm of the Y chromosome. Recently, a region termed $quot;SRY$quot;, a single copy gene of the Y chromosome was identified as belonging to a testis-determining gene. This gene is Y-specific, highly conserved among mammals, and transcribed only in testis. The predicted amino acid sequence of SRY shares homology with DNA-binding domains of transcription factors such as chromatinassociated, nonhistone proteins HMG 1 and HMG 2. Hence, it was thought that there may be some change in SRY gene of the patients with Swyer syndrome. And it was reported in some cases that there was deletion or mutation in the gene, but no abnormality of SRY gene was observed in other cases. So, it is a new approach to understand the mechanism of the human sexual differentiation to investigate this syndrome in terms of DNA sequence of SRY gene. To verify the presence or absence of SRY, or the change in SRY, we performed polymerase chain reaction and DNA sequencing of the conserved region of SRY gene from four patients with Swyer syndrome and their family members. The results are as follows. 1) Four patients with Swyer syndrome, their father, and two normal male control were positive whereas two female control were negative for the band that represents the coding sequence of SRY. 2) The DNA sequences of SRY gene from four patients with Swyer syndrome, their father, and two normal male control were the same, and there was no deletion or mutation in the gene. In conclusion, there may be complex sex determining cascade including other genes not only on the Y chromosome, but also on the X chromosome or even autosome in human sexual differentiation.