인간 위장관 종양 세포주에서 c - fos , c - Ha - ras , 그리고 라미닌의 유전자의 발현

최규실,서영아,김규원 ( Kyu Sil Choi,Young Ah Suh,Kyu Won Kim ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.6

We have investigated a correlation between oncogene and laminin expression and metastatic aggressiveness with three stomach (SNU-1, 5, and 16) and seven colon (SNU-61, 67, 70, C1, C2A, C4, and C5) carcinoma cell lines which were estabilished from primary and metastatic tumor sites. According to Northern blot analysis and immunofluorescence staining, the expression of c fos was elevated in SNU-C4 (primary, colon) and SNU-C5 (primary, colon). The expression of c-Ha-ras was increased in SNU-61 (metastatic, colon). In malignant progression, ras oncogene expression was believed to be responsible for tumor metastasis, whereas the mRNA of c-fos was expressed mainly in the low metastatic clones. In this regard, our results were somewhat in accordance with previous reports. On the other hand, laminin mRNA was detected in all cell lines tested, and among them higher level of laminin mRNA expression was observed in SNU-5 (metastatic, stomach), SNU-C2A (primary, colon) and SNU-C5 (primary, colon). This result suggests that the tumor metastatic behavior can not be simplified to laminin gene expression and the correlation between tumor metastatic aggressiveness and laminin gene expression is more complex.

Expression of c-fos, c-Ha-ras and Laminin in the Human Stomach and Colorectal Carcinoma Cell Lines

최규실,서영아,김규원,Choi, Kyu-Sil,Suh, Young-Ah,Kim, Kyo-Won 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.6

한국인 위장관 종양에서 확립된 3종류의 위암 세포주(SNU-1, 5, 16)와 7종류의 대장암 세포주 (SNU-61, 67, 70, C1, C2A, C4, C5)를 대상으로 하여 암유전자 및 라미닌 유전자의 발현양상과 종양전이 능력과의 상관관계를 조사하였다. c-fos 암유전자의 경우 일차종양에서 확립된 SNU-C4와 C5에서 발현이 높게 나타나고, c-Haras 암유전자인 경우는 전이 부위에서 확립된 SNU-61 세포주에서 발현이 높으므로 전이성이 낮은 세포주에서 c-fos의 발현이 높고, 전이성이 높은 세포주에서는 c-Ha-ras의 발현이 높다는 종래의 실험결과와 어느 정도 일치함을 나타내었다. 그러나 라미니 유전자인 경우는 SNU-5, C2A, C5 등의 세포주에서 비교적 높게 발현이 되었으나 다른 세포주에서도 고르게 발현이 되고 있으므로 라미닌 유전자의 발현과 종양전이 능력과의 상관성을 SNU 세포주에서는 얻기가 어려운 것으로 추정이 된다. We have investigated a correlation between oncogene and laminin expression and metastatic aggressiveness with three stomach (SNU-1, 5, and 16) and seven colon (SNU-61, 67, 70, C1, C2A, C4, and C5) carcinoma cell lines which were estabilished from primary and metastatic tumor sites. According to Northern blot analysis and immunofluorescence staining, the expression of c fos was elevated in SNU-C4 (primary, colon) and SNU-C5 (primary, colon). The expression of c-Ha-ras was increased in SNU-61 (metastatic, colon). In malignant progression, ras oncogene expression was believed to be responsible for tumor metastasis, whereas the mRNA of c-fos was expressed mainly in the low metastatic clones. In this regard, our results were somewhat in accordance with previous reports. On the other hand, laminin mRNA was detected in all cell lines tested, and among them higher level of laminin mRNA expression was observed in SNU-5 (metastatic, stomach), SNU-C2A (primary, colon) and SNU-C5 (primary, colon). This result suggests that the tumor metastatic behavior can not be simplified to laminin gene expression and the correlation between tumor metastatic aggressiveness and laminin gene expression is more complex.

Hypoxia-induced Angiogenesis during Carcinogenesis

최규실,배문경,정주원,문효은,김규원,Choe, Gyu-Sil,Bae, Mun-Gyeong,Jeong, Ju-Won,Mun, Hyo-Eun,Kim, Gyu-Won Korean Society for Biochemistry and Molecular Biol 2003 Journal of biochemistry and molecular biology Vol.36 No.1

The formation of new blood vessels, angiogenesis, is an essential process during development and disease. Angiogenesis is well known as a crucial step in tumor growth and progression. Angiogenesis is induced by hypoxic conditions and regulated by the hypoxia-inducible factor 1 (HIF-1). The expression of HIF-1 correlates with hypoxia-induced angiogenesis as a result of the induction of the major HIF-1 target gene, vascular endothelial cell growth factor (VEGF). In this review, a brief overview of the mechanism of angiogenesis is discussed, focusing on the regulatory processes of the HIF-1 transcription factor. HIF-1 consists of a constitutively expressed HIF-1 beta(HIF-1β) subunit and an oxygen-regulated HIF-1 alpha(HIF-1α) subunit. The stability and activity of HIF-1α are regulated by the interaction with various proteins, such as pVHL, p53, and p300/CBP as well as by post-translational modifications, hydroxylation, acetylation, and phosphorylation. It was recently reported that HIF-1α binds a co-activator of the AP-1 transciption factor, Jab-1, which inhibits the p53-dependent degradation of HIF-1 and enhances the transcriptional activity of HIF-1 and the subsequent VEGF expression under hypoxic conditions. ARD1 acetylates HIF-1α and stimulates pVHL-mediated ubiquitination of HIF-1α. With a growing knowledge of the molecular mechanisms in this field, novel strategies to prevent tumor angiogenesis can be developed, and form these, new anticancer therapies may arise.

치위생과 학생의 2×2 성취목표지향성이 자기조절학습에 미치는 영향

최규일 ( Gyu Yil Choi ),정기옥 ( Gi Ok Jung ) 한국치위생과학회 2012 치위생과학회지 Vol.12 No.4

This research will target the Dental Hygienics students to identify the level and type of the 2×2 achievement target orientation, and will study how this achievement target orientation is related to the Self-Regulated Learning, as well as the effect on the each sub elements of the Self-Regulated Learning (SRL). Among the 2×2 achievement target orientation of all the female university students, the skillful approach was found to be highest. In case of the adjustment of the motivation following grade, intrinsic value of the 1st grader was higher than the intrinsic value of the 2nd and 3rd graders. As for the behavior adjustment, the 3rd grader`s time and studying adjustment were found to be higher. Mean while, pursuit of the cooperation was found to be high compared to the time and studying adjustment of the1st and 2nd graders. Second, intrinsic value, overt goal orientation, and studying environment adjustment among the SRL`s subelements, manifested significant correlation with all the sub elements of the 2×2 achievement target orientation. As for the elements that affected cognition adjustment, grade and skillful approach were found to exert significant effect on the performance adjustment. As for the element that affects behavior adjustment, grade and skillful approach exerted significant effect on the sub elements of the behavior adjustment. Analysis on the effect of the achievement target orientation and SRL implies that the direction of the students` learning goal can be modified and that they can learn effectively by using the SRL appropriately. When the two elements are factored in carefully, the key findings could serve as a base data that can motivate the students, inducing effective learning process.

졸-겔법을 이용한 TiO₂ 박막의 광촉매 특성

최규만(Choi, Kyu-Man),김여환(Kim, Yeo-Hwan),임해진(Lim, Hae-Jin) 한국산학기술학회 2013 한국산학기술학회논문지 Vol.14 No.2

TiO₂ 박막을 저온 열처리 졸-겔 법으로 합성하였다. 박막의 기판은 면적이 100㎟인 붕규산염 유리를 사용 하여 시료를 300˚C부터 11000C까지 열처리하였고, 이때 제조된 박막의 두께는 약 1.5μm정도였다. 300˚C에서 2시 간 동안 열처리한 TiO₂ 박막은 아나타제 상을 나타내었고 열처리 온도가 증가함에 따라 비정질 상태에서 아나타제 상과 루타일 상이 공존하면서 각 상의 분율이 변화하였다. SEM 분석에 의하면 박막의 입자 크기는 0.1∼0.54μm이었 으며 Uv-visible 반사특성에 있어서 390nm부근에서 광흡수가 되는 것을 알 수 있었다. 따라서 낮은 열처리 온도에서 생성된 TiO₂ 박막은 주로 아나타제 상을 가지며 광촉매 특성을 2.4배 증가시키는 것으로 나타났다. Thin film of TiO₂ was obtained by the sol-gel dip method on the brosilicate glass substrate. It was found that the film was about 1.5μm thick as obtained by 4 successive coatings and annealed at varied temperatures ranged from 300˚C to 1100˚C for 2 hrs. The substrate used was having the surface area of 100㎟. Increasing the annealing temperature caused to change in mineralogical phase of titanium oxide i.e.., amorphous, crystalline antase to rutile phases. The particle size of the titanium oxide film were ranged from 0.1~0.54μm estimated by the SEM analysis. The material showed an absorbance maximum at the wavelength 390nm obtained by UV-visible spectrophotometer. These results therefore, indicated that the TiO₂ film obtained relatively at low annealing temperature consisted predominantly with anatase phase; possessed higher photocatalytic behavior i.e., 2.4 times higher than that of only UV lamp irradiation.

신교육목표분류학의 틀에서 본 구강보건교육학 목표 분석

최규일 ( Gyu Yil Choi ),최병욱 ( Byung Ok Choi ) 한국치위생과학회 2012 치위생과학회지 Vol.12 No.3

This study examined in what category that the knowledge dimension and the cognitive processes dimension are described based on ``A revision o Bloom``s taxonomy of educational objectives`` in oral health education textbooks. Oral health education and practice from Educational Objectives of Dental Hygiene Department(Korea dental hygienist Prof Council, 2009) were selected to analyze a textbook, the body contents was analyzed in the knowledge dimension, and the activity was analyzed in cognitive processes dimension. Three experts were selected as a corder for reliable analysis. As a result of this study, the knowledge dimension in oral health education textbook was focused on the categories of factual knowledge and conceptual knowledge in textbook analysis based on ``A revision of Bloom``s taxonomy of educational objectives``, and the category dimension of ``understand`` showed to be the most for the cognitive processes dimension. Moreover, there was no ``meta cognitive knowledge`` that conforms to a higher-order thinking and the category dimensions of ``analyze``, ``evaluate``, and ``create`` took very low proportion or did not exist. Conclusion, Oral health education textbooks were analyzed to fragmentary and Memorizing the level knowledge. Thus we have to develop oral health education textbooks reflected a variety of cognitive and knowledge dimension.

자율주행 DRT(수요응답형 교통) UX 디자인 특성 연구: 고령자를 중심으로

최규한(Kyu-Han Choi) 한국콘텐츠학회 2021 한국콘텐츠학회논문지 Vol.21 No.12

본 연구는 2027년 적용 가능한 레벨5의 자율주행차량을 기반으로 자율주행 DRT UX 디자인 특성을 제안하는데 목적이 있다. 연구범위로는 고령자와 차량 내외의 인터랙션을 중심으로 한 시스템으로 하였으며, 레벨 5의 자율주행차량을 연구대상으로 하였다. 적용 대상으로는 2021년을 기준으로 60대부터 90대로 설정하였다. 본 연구는 고령자와의 직접적인 소통을 통한 실제적인 인사이트를 도출한 자율주행 DRT UX 디자인 특성 연구라는데 기존 연구와의 차별성이 있다. 연구 방법으로는 문헌연구를 통해 자율주행차량과 DRT를 이론적으로 고찰하였으며, 이를 바탕으로 자율주행차량과 DRT의 사례를 분석하였다. 사례연구로는 고령자 인터뷰, 자율주행차량 시승, 영상제작, 설문조사, 고령자 자율주행차량 VR 시승을 통한 일반화로 진행하였다. 포커스 그룹 인터뷰(FGI)를 통해 자율주행 DRT UX 디자인 특성 10가지를 도출하였으며, 도출된 특성은 예약, 승차, 입력, 주행, 응급, 하차 등으로 구분되었다. 본 연구를 통해 고령자의 이동성 향상에 이바지하고 자율주행 DRT의 실용화를 한층 앞당기는 계기가 되기를 바란다. The purpose of this study is to propose the features of autonomous DRT UX design based on level 5 autonomous vehicle applicable in 2027. As the scope of the study, the system focused on the interaction between the elderly and the in/outside of the vehicle, and the level 5 autonomous vehicle was the subject of the study. As of 2021, the targets for application were set from 60s to 90s. This study is different from previous studies in that it is a study on the features of autonomous DRT UX design that derives practical insights through direct communication with the elderly. As a research method, autonomous vehicle and DRT were theoretically considered through literature research, and based on this, cases of autonomous vehicle and DRT were analyzed. As a case study, generalization was conducted through interviews with the elderly, test-drive of autonomous vehicle, video production, survey, and VR test-drive of autonomous vehicle for the elderly. Ten features of autonomous DRT UX design were derived through focus group interview(FGI), and the derived features were classified into reservation, get on, input, driving, emergency, and get off. Through this study, I hope that it will contribute to improving mobility of the elderly and further accelerate the practical use of autonomous DRT.

미생물 연료전지의 전극 재료와 구조에 따른 전기적 특성

최규만,Choi, Kyu-man 한국정보전자통신기술학회 2014 한국정보전자통신기술학회논문지 Vol.7 No.1

폐수 속에 들어있는 미생물을 이용해서 전기를 얻을 수 있도록 고안된 것이 미생물 연료전지이다. 본 논문에서는 미생물 연료전지의 전극 재료와 구조에 따른 전기적 특성을 조사했다. 구리판을 캐소드 전극으로 사용한 연료전지는 구리판의 산화 반응에 따른 전압역전현상이 관찰되어 백금판을 전극으로 사용한 연료전지보다 낮은 출력 전압을 나타내었다. 구리판을 전극으로 사용한 경우 전극판의 간격이 작을수록 높은 전압특성을 나타내었고 면적이 넓을수록 최고출력전압이 나타나는 시간이 지연되는 특성을 보였다. MFC(microbial fuel cell) is the device to produce the electricity by using the microbes which are living in the waste water. In this paper, the electric characteristics of the MFC were investigated according to each different structure and electrode materials. The voltage being reversed phenomenon was observed in the MFC which uses the cupper plate as the cathode material. This result comes from the oxidation reaction of the cupper plate electrode in this MFC. And this MFC has lower output voltage than one that has a platinum plate electrode. The smaller gap distance of the cupper plate electrode of the MFC showed the higher output voltage. The larger electrode area of the cupper plate electrode showed that the reaching time of the output voltage to the maximum value was delayed.

산업장 근로자의 구강보건 정의적 요인이 구강건강 행위와 구강건강 삶의 질에 융합적으로 미치는 영향

최규일,김경미,윤현경 한국융합학회 2020 한국융합학회논문지 Vol.11 No.2

The purpose of this study was to examine the effect that the affective factors of oral health in industrial workers would have on oral health behaviors and quality of oral health life. A survey was performed of 100 subjects selected from 5 industrial companies located in Chungcheongbuk-do through simple random sampling method from January 19 to 23, 2018, using the questionnaires. The data collected from the survey were analyzed with frequency analysis, independent t-test, and multiple regression analysis by using the SPSS 18.0 statistical program. The results showed that the affective factors of oral health had an influence on oral health behaviors and that the influence on quality of oral health life was greater when the oral health practice intention was stronger. Based on aforesaid results, effective oral health programs, conducive to bringing changes to the affective factors of oral health, would need to be developed in order to enhance oral health behaviors and quality of oral health life in industrial workers. 본 연구는 산업체 근로자의 구강보건 정의적 요인이 구강건강 행위와 구강건강 삶의 질에 미치는 영향을 파악하고자 하였다. 충북지역 산업체 5곳 100명을 대상으로 2018년 1월 19일부터 23일까지 단순무작위 표본추출방법으로 선발하여 설문지법으로 조사하였고, 자료분석은 SPSS 18.0을 이용하여 빈도분석, 독립 T검정, 다중회귀분석을 실시하였다. 그 결과, 구강보건 정의적 요인이 구강건강 행위에 영향을 미치는 것으로 나타났고, 구강보건 실천의지가 높을수록 구강건강 삶의 질에 영향을 미치는 것으로 나타났다. 이를 통해 산업장 근로자의 구강건강 행위와 구강건강 삶의 질을 증진시키기 위해서는 구강보건 정의적 요인을 변화 시킬 수 있는 효율적인 구강건강프로그램이 개발되어야 한다.

하소조건이 YAG:Ce형광체 특성에 미치는 영향

최규만(Kyu-Man Choi),김재문(Jae-Moon Kim),이준모(Joon-Mo Lee) 대한전자공학회 2010 대한전자공학회 학술대회 Vol.2010 No.6