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      • KCI등재

        Synthesis and Charactrization of Polycaprolactone Nanocomposites Reinforced with Montmorillonite

        조성준 한국세라믹학회 2004 한국세라믹학회지 Vol.41 No.6

        [DEACOOH]-Montmorillonite intercalations complex obtained from Na-Montmorillonite and 10-Carboxy-n-decyldimethylethylammonium bromide(organic cation) was reacted with the monomer(ε-caprolactone) to achieve the [DEACOOH]-ε-caprolactone-Montmorillonite intercalations complex. From this intercalations complex Montmorillonite/Polycaprolactone Nanocomposites in which montmorillonite(inorganic polymer) is chemically linked with the polycaprolactone(organic polymer) were formed at 240oC by three different methods such as in stoichiometric amounts between monomer and organic cation, in excess of only the monomer and in excess of both organic cation and monomer. The products obtained after polymerization were analyzed with X-ray diffractometer and TEM.

      • Heterogeneous nuclear RNP A1 protein methylase Ⅰ의 분자적 특성에 관한 연구

        조성준,유총근,박길홍,김상덕 고려대학교 의과대학 1997 고려대 의대 잡지 Vol.34 No.3

        Al protein is a nucleic acid binding protein present in nucleoplasm, known to have certain roles in DNA replication and mRNA posttranscriptional processing binding to ssDNA and ssRNA, and reportedly methylation of arginine residue of this protein decreases RNA/protein interaction. Present study was designed based on the hypothesis that methylation of the enzyme act as regulatory mechanism of DNA replication as well as mRNA processing by modulating physiological activity of Al protein. For the purpose, the enzyme purification was performed to identify molecular characteristics and enzymatic properties, and amino acid composition and partial sequence was determined as an initial step for cloning. Results obtained are as follows. 1) Al protein methylase I was purified to apparent homogeneity. 2) Molecular weight is 450 kD, and composed of tetramer of 4 idential subunits. 3) Catalytic activity was still present in dimeric and trimeric form of the enzyme. 4) Substrate specificity for the enzyme was highest in Al protein, that of histone at about half level, and other protein substrate including bovine serum albumin, myelin basic protein, and γ-globulin negligible. 5) Effect of divalent cations on the enzyme activity was observed with Ca ²^(+), Mg ²^(+), Cu ²^(+), Fe ²^(+). No significant effect was observed implying those are not plausible to act as cofactor for the enzyme. 6) Amino acid composition of the purified enzyme indicated relatively high content of glycine (14 mol%) and leucine (11 mol%). Results obtained from the present studies on molecular characteristics of Al-PMI will provide bases for cloning the methylase gene, and enable us to investigate further on DNA replication and mRNA processing as well as its regulatory mechanisms mediated by hnRNP protein utilizing molecular biological techniques. Furthermore, these knowledges will provide us to better understand mechanisms not-yet uncovered during cell proliferation and differentiation, the major topic of modern biology.

      • KCI등재

        다시 살펴보는 수술실 CCTV

        조성준 한국의료윤리학회 2022 한국의료윤리학회지 Vol.25 No.3

        수술실에서 연이어 일어난 비윤리적 사건들은 한국 사회에 큰 충격을 주었다. 이에 감시가 필요하다는 사회적 공감대가 형성되었고 수술실 내부에 CCTV(Closed Circuit Television)를 강제하는 의료법 개정이 이루어졌다(<부록 1> 의료법 제38조의2). ‘수술실 CCTV 설치 의무화’ 찬성의 근거는 명확하다. 수술실의 일탈 행위는 사회가 용인할 수 있는 한계를 넘어섰고 이를 방지할 대책으로 CCTV 외에 다른 대안이 없다는 것이다. 의사들의 비윤리적 행위가 일으킨 대중의 분노는 엄청난 것이어서 여론을 돌이켜 세우는 것은 처음부터 불가한 것이었다. 수술실 CCTV는 ‘의사들의 도덕성에 가해진 사회적 메스’ 였다. 이미 법률안이 확정되었음에도 다시 살펴봐야 할 내용들이 있다. 첫째, 감시와 관련된 인권의 영역을 살펴보는 작업이다. 1) 노동감시와 직업수행의 자유, 2) 프라이버시, 3) 개인정보자기결정권 등이 그것이다. 수술실의 감시 카메라는 불법적 행위로부터 환자 안전을 지키는 강력한 수단이지만 의료진의 기본권을 제한한다. ‘감시에 의한 시민 보호와 인권 침해’, 충돌하는 두 가치 사이에서 우리 사회는 합의점을 찾아야 한다. 둘째, 영상정보관리의 한계와 위험성이다. 시행상 가장 중요한 문제로 법안의 시행령, 규칙을 세밀히 다듬어 피해를 최소화해야 한다. 셋째, 한국 의료 내부의 구조적 문제들이다. 입법 과정에서 충실히 논의되지 않은 복잡한 사안들로 환자 안전을 위협하고 갈등을 유발하는 근본적 요인이기도 하다. 수술실 CCTV 시행이 내년으로 다가왔다. 환자 안전을 도모하고 부작용을 최소화하려면 이상의 문제들을 더 깊이 살펴보고 시작해야 한다.

      • 日本의 發射豫定인 放送衛星이 우리나라에 끼칠 수 있는 影響에 對한 考察 : 放送衛星에 依한 宇宙通信의 國際法的 側面을 中心으로 According to the International Law of Space Telecommunications by a Broadcasting Satellite

        趙成俊 한국항공대학교 1976 論文集 Vol.10 No.-

        通信衛星의 送信電力이 數W내지 10w정도인데 반해 放送衛星은 數10w내지 數100w로 대단히 커서 自國의 特定地域外에 누설되는 電波는 地國의 無線通信에 障害를 주게 된다. 따라서 放送衛星의 送信안테나의 指向性 여하가 技術的 問題外에도 法律的, 政治的 또는 社會的 問題로서 國際間의 紛爭의 한 原因이 되고 있다. WARC-ST에서도 이를 爲해 相對國間 또는 多數國間의 條約締結에 依한 [明示된 事前同意]의 必要性을 決議했다. 日本이 發射 豫定하고 있는 實驗用 放送衛星도 경우에 따라 그의 Coverage속에 우리나라의 一部가 포함될수 있다. 따라서 우리나라는 宇宙通信의 國際法的인 側面에서 이에 對한 對??을 硏究할 必要가 있음을 지적하고자 한다. The output of the broadcasting satellite, 10 watts or 100watts, is much larger than that of the communication satellite, a few watts or 10 watts. Therefore, the leakage waves from the broadcasting satellite except its own broadcasting region are affected on the tele-communications of the other radio stations. The debates on the directional range of its broadcasting antenna, not only a technical but also a judical and a social problem, are introduced to the issues between the nations. Recently WARC-ST determined the need of "A clear Agreement in advance"by their contract between the other nation for the above problems. A Japan's broadcasting satellite to be launched will may be included the sourthern parts of the Korean penisular in its coverage. Therefore, the authorities of Korea must have the counter-measures which the International Law of Tele-communications can be taken advantage of.

      • 대기부유분진중 nitroarenes 오염도와 그 돌연변이원성에 관한 조사연구 : 서울시 일부지역을 중심으로

        조성준,최용욱,임영욱,신동천,정용 한국환경독성학회 1990 환경독성보건학회지 Vol.5 No.2

        Nitroarenes are ubiquitous environmental pollutants displaying potent mutagenicity in bacteria and carcinogenicity in mammal. In this study, the concentration of nitroarenes in coarse and fine particles and mutagenicity of POC_(N) fraction was investigated in suspended particulates at the Shinchon and Bulkwang area of Seoul. The suspended particulates were collected bimonthly by a high volume cascade impactor air sampler from July 1987 to May 1988. Extractable organic matter was obtained by ultrasonic extraction on diethly ether/cyclohexane (8/2, v/v). Neutral fraction was obtained by liquid-liquid extraction. Polar neutral organic compounds (POC_(N)) was fractionated by thin-layer chromatography. Finally, the concentrations of nitroarenes in POC_(N) fraction were measured and determined by capillary gas chromatography. Direct and indirect mutagenicity of POC_(N) fraction were measured using Salmonella typhimurium TA 98. The result were as follows: 1) Major nitroarenes at the Shinchon area was 1-nitropyrene and at the Bulkwang area it was 2,7-dinitro-9-fluorenone during the year. 2) Average concentration of total nitroarenes measured was 67.26 ng/m³ in fine particles which was 1,3 folds higher that in coarse particle (52.30 ng/m³). 3) Annual pattern of nitroarenes concentrations revealed that concentration during heating season (Feb., Jan., Mar.) was 2.2 folds higher than that in non heating season (May, Jul., Sep.). Concentration of each season has 157.68ng/m³ and 80.39 ng/m³. 4) The mutagenic activity of POC_(N) fraction from fine particles was higher compared to that of coarse particles and was increased when metabolically activated, with S9 mixture. Mutagenicities, Metabolically activated, were significantly different between Shinchon and Bulkwang area, 322.8 rev/250㎍/plate and 286.8rev/250㎍/plate, respectively. 5) Annual pattern of mutagenicity of POC_(N) fraction revealed that mutagenicity during the heating season was 1.7 folds higher at Shinchon area and 1.2 folds higher at Bulkwang than during the non heating season. The variable contents and levels of nitroarenes in suspended particulates may affect human health significantly. Further studies such as risk assessment should be conducted on the basis of these kind of studies.

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