담수산 진주패 뻘조개 , Anodonta(Sinanodonta) woodiana(Lea) 의 생식주기 및 임란기

정의영 한국수산학회 1980 한국수산과학회지 Vol.13 No.4

The reproductive cycle and the breeding season of the freshwater clam, Andonta (Sinanodonta) woodiana (Lea) have been investigated by histological examination of the gonadal development under photomicroscopy. The materials were monthly collected from the Nakdong River for one year from sptember 1979 to August 1980. Sexuality of Andonta (Sinanodonta) woodiana is dioecious, and the species are ovoviviparouts. The gonads are irregularly arranged from the subregion of mid-intestinal gland in visceral cavity to reticular connective tissue of foot. The ovary is composed of a number of small ovarian sacs. The epithelium of ovarian sac has a function of the germinal epithelium. Oogonia actively proliferate along the germinal epithelium of the ovarian sac, in which young oocytes are growing. The testis is composed of a number of seminiferous tubules, and the epithelium of the tubule has function of germinal epithelium, along which spermatogonia actively proliferate. A great number of undifferentiated mesenchymal tissue and eosinophilic granular cells are abundantly distributed between the growing oocytes and spermatocytes in the early development stages. With the further development of the ovary and testis these tissues and cells gradually disappear. Then the undifferentiated mesenchymal tissue and granular cells are considered to be related to the growing of the oocytes and spermatocytes. The gonads had function year-round the individuals which have various developmental stages of gonads appearing all the time. Spawning continued year-round except for the period of high temperature of water, during August and September. The peak spawning seasons appeared twice a year between January and March, and between June and July in 1980. Individuals which have trochophore larvae in the marsupium of the adult appeared year-round except September 1979 and August 1980. The rate of individuals which have glochidia in the marsupium was 72.7 percent in May 1980 which was the highest brooding rate.

자연산 바지락, Ruditapes philippinarum의 생식주기와 수온-먹이섭이 및 절식조건에 의한 생식소발달의 인위적 제어

정의영,이정식,이창훈,허성범 한국패류학회 2002 The Korean Journal of Malacology Vol.18 No.2

짱뚱어, Boleophthalmus pectinirostris의 성 성숙

정의영 부산수산대학 해양과학연구소 1991 연구논문집-부산수산대학교 해양과학공동연구소 Vol.23 No.-

새만금호의 수질예측과 그에 따른 대책 1. 환경 오염원이 참재첩 ( Corbicula Leana ) 의 여수작용 및 산소소비에 미치는 영향

정의영,신윤경,최문술 한국패류학회 1997 The Korean Journal of Malacology Vol.13 No.2

As a prioiminary study on usage of metabolic charateristics of the indicator species for indirect estimation of environmental water quality, effects of environmental pollutants on survival, filtration and oxygen consumption rates in Corbicula aeana were investigated at 17$^{\circ}C$ and $25^{\circ}C$ in 10 day afrer treatmint of pollutants. In case of glucose and complex fertilizer, the survival rates of the clams were 100% without any relation to individual sizes and water temperatures. In small sizes at $25^{\circ}C$, the survival rates of the clams by NH$_{4}$CI concentration were shown 95% at 10 mg/1 and 15mg/1, and 90% at 20mg/1, respectively. But the survival rate was 95%at 20 mg/1 of NH$_{4}$CI concentration in small size at 17$^{\circ}C$. The higher filtration and oxygin consumption rates were shown in small size at higher water timperatures(over $25^{\circ}C$), and generally filtration and oxygen consumption rates decreased with increase of glucose, complex fertilizer and NH$_{4}$CI concentrations, respectively. In general, effects of filtration and oxygen consumption rates at NH$_{4}$CI concentrations were shown slightly larger than those of glucose and complex fertilizer.

Ultrastructural Study of the Process of Oocyte Degeneration and Function of the Follicle Cells in Female Spisula sachalinensis on the East Sea of Korea

정의영,이기영,이정용 한국패류학회 2008 The Korean Journal of Malacology Vol.24 No.1

Ultastructural studies of oocyte degeneration and follicle cells in female Spisula sachalinensis are described for clams collected from Jumunjin, Gangwondo, Korea. The follicle cells playan integral role in vitellogenesis and oocyte degeneration by assimilating products originating from the degenerated oocytes (thus allowed the transfer of yolk precursors needed for vitellogenesis). The functions of the follicle cells include phagocytosis and intracellular digestion of products originating from oocyte degeneration. During the period of oocyte degeneration, follicle cells of this species probably have lysosomal systems for the breakdown and reabsorption of various phagosomes(phagolysosomes) in the cytoplasm for nutrient storage; this process has been observed in other bivalves.

바지락의 산소비율 및 여수율의 생리적 리듬

정의영,신윤경,허성범 한국패류학회 1999 The Korean Journal of Malacology Vol.15 No.2

전북 곰소만에 서식하는 바지락의 생리적 리듬을 알아보기 위하여 24시간 동안 1시간 간격으로 산소소비율 및 여수율의 변화를 측정하였다. 바지락의 호흡율 및 여수율에 의한 생리적 리듬은 15$^{\circ}C$와 $25^{\circ}C$에서 두 가지 모두 24시간 동안 2번의 peak를 보이는데, 밤-만조시에 최대를 나타내었으며, 낮-간조시에 최소를 나타내어 조석에 의한 일주리듬 현상을 보였다. 24시간 동안 산소소비율 및 여수율의 변화는 수온별로 급격한 특수한 변화를 보이지 않았다. Changes in Oxygen consumption and filtration rates were investigated to understand physiological rhythms for 24 hours of the Manila clam, Ruditapes philippinarum. physiological rhythms in the oxygen consumption and filtration rates at 15$^{\circ}C$ and 25$^{\circ}C$ were showed diurnal tidal rhythms, appearing two peaks for 24 hours: maximum at night-high tide and minimum at day-low tide. No rapid variations in oxygen consumption and filtration rates for 24 hours appeared at two different water temperatures.

전복, Nordotis discus 의 호흡과 폐사에 미치는 부니의영향

정의영,신윤경,이주하 한국패류학회 1993 The Korean Journal of Malacology Vol.9 No.2

전복, Nordotis discus의 1$0^{\circ}C$와 2$0^{\circ}C$의 수온구에서 개체크기별 대사와 폐사에 미치는 부니의 영향에 대한 결과는 다음과 같다.1. 수온 -염분별 산소소비율의 일반적 경향은 고온-고염분에서 큰개체보다는 작은개체에서 그 비율리 높게 나타났다.2. 염분별 두 수온간의 온도계수 (Q $_{10)}$ 는 작은 개체에서 1.2-2.9의 병위를 큰개체의 경우 1.5-3.4의 범위를 나타내었으나 개체크기에 관계없이 유사한 양상을 보였다.3. 부니의 농도별 사망율은 작은 개체의 경우 처리 96시간후 2$0^{\circ}C$수온에서 부니 농도1000ppm에서 가장 높았고. 큰 개체의 경우 50ppm에서 가장 낮았다.4. 96시간-반치사농도(LC$_{50}$)는 1$0^{\circ}C$의 경우, 큰개체에서 1076.58 ppm, 작은 개체에서 812.55 ppm이었으며, 2$0^{\circ}C$의 경우는 큰개체에서 698.28 ppm, 작은 개체에서 546.74ppm으로 나타났다.5. 부니의 영향으로 인한 호흡산소소비량은 개체크기에 관계없이 1$0^{\circ}C$에서 보다 2$0^{\circ}C$에서 더욱 높았으며, 1000ppm에서 70% 이상의 감소율을 나타내었다.

Ultrastructure of Oocytes During Oogenesis and Oocyte Degeneration Associated with Follicle Cells in Female Sinonovacula constricta (BIVALVIA: PHARIDAE) in Western Korea

정의영,고철환,강희웅,최기호,전제천 한국통합생물학회 2008 Animal cells and systems Vol.12 No.4

The ultrastructure of oocytes during oogenesis and oocyte degeneration associated with follicle cells in female Sinonovacula constricta (Lamarck, 1818) were investigated by electron microscope observations. Ovarian follicles are surrounded by a matrix of vesicular connective tissue cells (VCT cells). VCT cells contain large quantities of glycogen particles and several lipid droplets in their cytoplasm. It is suggested that VCT cells act as a source of nutrients for vitellogenesis during oogenesis. In early vitellogenic oocytes, several coated vesicles, which appear at the basal region of the oocyte, lead to the formation of membrane-bound vesicles via endocytosis. The uptake of nutritive materials in coated vesicles formed by endocytosis appears through the formation of coated pits on the oolemma during vitellogenesis. During the late stage of oogenesis, yolk precursors (yolk granules), mitochondria and lipid droplets are present in the cytoplasm of late vitellogenic oocytes. In particular, proteinaceous yolk granules containing several different components are intermingles and form immature yolk granules. In the mature oocyte, small immature yolk granules are intermingled and form large mature yolk granules. Vitellogenesis occurs through a process of autosynthesis, involving combined activity of the Golgi complex, mitochondria and rough endoplasmic reticulum in the cytoplasm of vitellogenic oocytes. The process of heterosynthesis is where extraovarian precursors are incorporated into oocytes by endocytosis at the basal region of early vitellogenic oocytes before the formation of the vitelline coat. Follicle cells appear to play an important role in vitellogenesis and oocyte degeneration. The functions of attached follicle cells to the oocyte during oocyte degeneration are phagocytosis and digestion of phagosomes originating from oocyte degeneration. After digestion of phagosomes, it is assumed that the function of follicle cells can permit a transfer of yolk precursors necessary for vitellogenesis and allows for the accumulation of glycogen and lipid during oocyte degeneration, which can be employed by vitellogenic oocytes. Follicle cells of S. constricta may possess a lysosomal system for induction of oocyte breakdown and might resorb phagosomes in the cytoplasm for nutrient accumulation during oocyte degeneration. The ultrastructure of oocytes during oogenesis and oocyte degeneration associated with follicle cells in female Sinonovacula constricta (Lamarck, 1818) were investigated by electron microscope observations. Ovarian follicles are surrounded by a matrix of vesicular connective tissue cells (VCT cells). VCT cells contain large quantities of glycogen particles and several lipid droplets in their cytoplasm. It is suggested that VCT cells act as a source of nutrients for vitellogenesis during oogenesis. In early vitellogenic oocytes, several coated vesicles, which appear at the basal region of the oocyte, lead to the formation of membrane-bound vesicles via endocytosis. The uptake of nutritive materials in coated vesicles formed by endocytosis appears through the formation of coated pits on the oolemma during vitellogenesis. During the late stage of oogenesis, yolk precursors (yolk granules), mitochondria and lipid droplets are present in the cytoplasm of late vitellogenic oocytes. In particular, proteinaceous yolk granules containing several different components are intermingles and form immature yolk granules. In the mature oocyte, small immature yolk granules are intermingled and form large mature yolk granules. Vitellogenesis occurs through a process of autosynthesis, involving combined activity of the Golgi complex, mitochondria and rough endoplasmic reticulum in the cytoplasm of vitellogenic oocytes. The process of heterosynthesis is where extraovarian precursors are incorporated into oocytes by endocytosis at the basal region of early vitellogenic oocytes before the formation of the vitelline coat. Follicle cells appear to play an important role in vitellogenesis and oocyte degeneration. The functions of attached follicle cells to the oocyte during oocyte degeneration are phagocytosis and digestion of phagosomes originating from oocyte degeneration. After digestion of phagosomes, it is assumed that the function of follicle cells can permit a transfer of yolk precursors necessary for vitellogenesis and allows for the accumulation of glycogen and lipid during oocyte degeneration, which can be employed by vitellogenic oocytes. Follicle cells of S. constricta may possess a lysosomal system for induction of oocyte breakdown and might resorb phagosomes in the cytoplasm for nutrient accumulation during oocyte degeneration.

조피볼락(Sebastes schlegeli)의 성숙에 따른 간세포 활성과 간 및 근육의 단백질 *

정의영 부경대학교 수산과학연구소 1995 硏究業績集 Vol.20 No.-

쥐노래미 Hexagrammos otakii의 난소 발달단계에 따른 간세포의 활성변화

정의영,강희웅,이근우 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.2

암컷 쥐노래미(Hexagrammos otakii)의 생식소중량지수(GSI)는 9월에 증가되기 시작하여 11월에 최대에 도달하였다. 쥐노래미의 생식주기는 연속적인 5단계로 나눌 수 있었다: 성장전기(7월), 성장후기(7~8월), 성숙기(9~10월), 완숙 및 산란기(9~12월), 회복 및 휴지기(12~6월). 간중량지수(HSI)의 월변화는 GSI변화와 유사한 경향을 보였다. 성장전기에 다량의 지방적과 글리코겐 입자들이 간세포내에 존재하였다. 특히, 이 시기에 간세포는 헤마톡실린에 약하게 염색되었다. 성장후기부터 성숙기에 간세포의 크기는 증대되었으며 간세포질내 글리코겐과 지방적들의 양은 감소된 반면, 호염기성 물질 축적이 증가하였다. 산란기부터 회복 및 휴지기에는 간세포내에 호염기성 물질 축적이 감소된 반면, 다량의 지방적과 글리코겐입자가 축적되었다. 간세포내의 총단백질, 총지질 및 글리코겐함량(%)의 최대값은 각각 9월(성숙기), 7월(성장전기), 3월(휴지기)에 나타났다. The GSI in the female greenling Hexagrammos otakii, began to increase in September and reached a maximum in November. The reproductive cycle of the greenling can be divided into five successive stage: early-growing stage(July), late- growing stage(July to August), mature stage(September to October), ripe-and-spent stages(September to December), and recovery-and-resting stages(December to June). Monthly changes in the HSI showed a similar pattern with those of the GSI. In the early- growing stage, a lot of lipid droplets, and glycogen particles were present in the cytoplasm of hepatocytes. Especially, the cytoplasm of hepatocytes were weakly stained with hematoxylin. In the late- growing to mature stage, hepatocytes became large, and the amount of glycogen and lipid droplets in hepatocytes gradually decreased, while basophilic substance increased. In the spawning to recovery-and-resting stages, basophilic substances decreased in the cytoplasm of hepatocytes. However, lots of lipid droplets and glycogen were stored in the hepatocytes. The maxium total protein, lipid and glycogen contents(%) in the hepatocytes appeared in September(mature stage), July(early-growing stage) and March(resting stage), respectively.