The Efficacy and Safety of Collagen-I and Hypoxic Conditions in Urine-Derived Stem Cell Ex Vivo Culture

전소영,김현태,권세윤,김정식,김범수,유은상,권태균 한국조직공학과 재생의학회 2016 조직공학과 재생의학 Vol.13 No.4

Upper urinary tract-derived urine stem cells (USCs) are considered a valuable mesenchymal stem cell source for autologous cell therapy. However, the reported culture condition for USCs is not appropriate for large-quantity production, because cells can show limited replicativity, senescence, and undesirable differentiation during cultivation. These drawbacks led us to reconstitute a culture condition that mimics the natural stem cell niche. We selected extracellular matrix protein and oxygen tension to optimize the ex vivo expansion of USCs, and compared cell adhesion, proliferation, gene expression, chromosomal stability, differentiation capacity, immunity and safety. Culture on collagen type I (ColI) supported highly enhanced USC proliferation and retention of stem cell properties. In the oxygen tension analysis (with ColI), 5% O2 hypoxia showed a higher cell proliferation rate, a greater proportion of cells in the S phase of the cell cycle, and normal stem cell properties compared to those observed in cells cultured under 20% O2 normoxia. The established reconstituted condition (ColI/hypoxia, USCsrecon) was compared to the control condition. The expanded USCsrecon showed highly increased cell proliferation and colony forming ability, maintained transcription factors, chromosomal stability, and multi-lineage differentiation capacity (neuron, osteoblast, and adipocyte) compared to the control. In addition, USCsrecon retained their immune-privileged potential and non-tumorigenicity with in vivo testing at week 8. Therefore, reconstituted condition allows for expanded uUSC cell preparations that are safe and useful for application in stem cell therapy.

Human Amniotic Fluid Stem Cell-derived Muscle Progenitor Cell Therapy for Stress Urinary Incontinence

전소영,Deok Hyun Cho,Seon Yeong Chae,Kyung Hee Choi,Hyun Ju Lim,Ghil Suk Yoon,김범수,Bup Wan Kim,James J Yoo,Tae Gyun Kwon 대한의학회 2012 Journal of Korean medical science Vol.27 No.11

The most promising treatment for stress urinary incontinence can be a cell therapy. We suggest human amniotic fluid stem cells (hAFSCs) as an alternative cell source. We established the optimum in vitro protocol for the differentiation from hAFSCs into muscle progenitors. These progenitors were transplanted into the injured urethral sphincter and their therapeutic effect was analyzed. For the development of an efficient differentiation system in vitro, we examined a commercial medium, co-culture and conditioned medium (CM) systems. After being treated with CM, hAFSCs were effectively developed into a muscle lineage. The progenitors were integrated into the host urethral sphincter and the host cell differentiation was stimulated in vivo. Urodynamic analysis showed significant increase of leak point pressure and closing pressure. Immunohistochemistry revealed the regeneration of circular muscle mass with normal appearance. Molecular analysis observed the expression of a larger number of target markers. In the immunogenicity analysis, the progenitor group had a scant CD8 lymphocyte. In tumorigenicity, the progenitors showed no teratoma formation. These results suggest that hAFSCs can effectively be differentiated into muscle progenitors in CM and that the hAFSC-derived muscle progenitors are an accessible cell source for the regeneration of injured urethral sphincter.

A Novel Dorsal Slit Approached Non-Ischemic Partial Nephrectomy Method for a Renal Tissue Regeneration in a Mouse Model

전소영,김대환,김정식,김현태,유은상,정재욱,하윤석,송필현,정윤기,한동근,정성광,김범수,권태균 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.4

BACKGROUND: Kidney ischemia–reperfusion (IR) via laparotomy is a conventional method for kidney surgery in a mouse model. However, IR, an invasive procedure, can cause serious acute and chronic complications through apoptotic and inflammatory pathways. To avoid these adverse responses, a Non-IR and dorsal slit approach was designed for kidney surgery. METHODS: Animals were divided into three groups, 1) sham-operated control; 2) IR, Kidney IR via laparotomy; and 3) Non-IR, Non-IR and dorsal slit. The effects of Non-IR method on renal surgery outcomes were verified with respect to animal viability, renal function, apoptosis, inflammation, fibrosis, renal regeneration, and systemic response using histology, immunohistochemistry, real-time polymerase chain reaction, serum chemistry, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, and Masson’s trichrome staining. RESULTS: The Non-IR group showed 100% viability with mild elevation of serum blood urea nitrogen and creatinine values at day 1 after surgery, whereas the IR group showed 20% viability and lethal functional abnormality. Histologically, renal tubule epithelial cell injury was evident on day 1 in the IR group, and cellular apoptosis enhanced TUNEL-positive cell number and Fas/caspase-3 and KIM-1/NGAL expression. Inflammation and fibrosis were high in the IR group, with enhanced CD4/CD8-positive T cell infiltration, inflammatory cytokine secretion, and Masson’s trichrome stain-positive cell numbers. The Non-IR group showed a suitable microenvironment for renal regeneration with enhanced host cell migration, reduced immune cell influx, and increased expression of renal differentiation-related genes and anti-inflammatory cytokines. The local renal IR influenced distal organ apoptosis and inflammation by releasing circulating pro-inflammatory cytokines. CONCLUSION: The Non-IR and dorsal slit method for kidney surgery in a mouse model can be an alternative surgical approach for researchers without adverse reactions such as apoptosis, inflammation, fibrosis, functional impairment, and systemic reactions.

Temporomandibular Disorders Symptoms Associated with Multicentric Reticulohistiocytosis: A Case Report

전소영,박지운 대한안면통증∙구강내과학회 2023 Journal of Oral Medicine and Pain Vol.48 No.2

This report describes the case of a 58-year-old woman who visited our outpatient clinic with the chief complaint of temporomandibular disorders (TMD) related symptoms and later reported a previous diagnosis of multicentric reticulohistiocytosis (MRH). The patient had a history of leg edema, pain, and skin lesions on the hands and knees, which were diagnosed as MRH by tissue biopsy. Temporomandibular joint pain and mouth-opening limitation developed 1 year ago. Radiographs of the maxilla and mandible revealed severe resorption of the mandibular condyle on both sides, resulting in the complete loss of the original shape of the condyle. The articular fossa was also affected, and consequently, the joint space was excessively widened. MRH is a rare non-Langerhans cell histiocytosis that primarily affects the skin and joints, causing skin nodules and joint pain along with bony deformity. Reports of involvement of tissues or organs other than the skin and joints of the extremities are limited, and to our knowledge, only one case of MRH with dental manifestation has been reported. Thus, this case report highlights the importance of considering MRH as a possible diagnosis in patients with TMD symptoms, particularly in those accompanied by severe bone resorption.

대기오염물질 배출량 연도별 변화(1984~2008)의 불확도 요인

전소영,김정,김영진,권진웅,허철행,장영기 한국대기환경학회 2012 한국대기환경학회 학술대회논문집 Vol.2012 No.5

모모제인을 위하여 - ‘누구’와 ‘우리’ 사이의 발명

전소영 천년의시작 2019 (계간)시작 Vol.18 No.2

[작품론] 혼자의 레퀴엠, 혼자들의 세레나데

전소영 계간 서정시학 2015 서정시학 Vol.25 No.2

노동의 행간, ‘없는 시간’ 으로서의 시

전소영 계간 서정시학 2019 서정시학 Vol.29 No.1

시-문지방의 주술에 관하여

전소영 천년의시작 2019 (계간)시작 Vol.18 No.3

수술 후 인지 기능 장애: 전임상 연구를 바탕으로

전소영,구본녀 대한마취통증의학회 2018 Anesthesia and pain medicine Vol.13 No.2

Postoperative cognitive dysfunction (POCD) occurs immediately after surgery and is characterized by impairment of memory and changes in cognition. POCD can last for several months or years and have adverse effects including delayed hospital stays, diminished function in daily life, and increased complications and mortality. Despite improvements in surgical technique, anesthesia management, and intensive care, many patients suffer from POCD. POCD is one of the important clinical issues in surgical management and understanding its pathophysiology is necessary. In this review, therefore, we have focused on animal models of POCD and measurements of cognitive ability in preclinical studies, and we have suggested novel approaches for prevention/treatment of POCD. In preclinical studies, major abdominal surgery (laparotomy, hepatectomy, and splenectomy), minor abdominal surgery (laparotomy, probe exploration), and tibial fracture surgery, are used as POCD models. In addition, cognitive function is assessed by Morris water maze, passive avoidance task, elevated plus maze, and T maze test. Neuroinflammation, blood-brain barrier dysfunction, beta amyloid deposition, and tau phosphorylation are suggested as pathological mechanisms of POCD in preclinical studies. Based on several studies of these, we suggest erythropoietin, nuclear factor kappa B, interleukin17A, tumor necrosis factor alpha, and nicotinamide adenine dinucleotide phosphate oxidase 2 as candidates for prevention/treatment of POCD. In the preclinical stage, drug development/exploration and research is being carried out to solve cognitive dysfunction after surgery. Ultimately, based on the results of preclinical studies, we expect to overcome POCD.