국내 거베라 육종계통 형질전환 기초 조건 확립

이혜영,이기정,전은희,신상현,이재헌,김도훈,정대수,정용모,조용조,김정국,정영수,Lee, Hye-Young,Lee, Ki-Jung,Jeon, Eun-Hee,Shin, Sang-Hyun,Lee, Jai-Heon,Kim, Doh-Hoon,Chung, Dae-Soo,Chung, Yong-Mo,Cho, Yong-Cho,Kim, Jeong-Kook,Chung, You 한국식물생명공학회 2006 식물생명공학회지 Vol.33 No.1

본 연구에서는 거베라 형질전환 기초기술개발을 위한 연구를 수행하였다. 경남원예화훼시험장에서 육성된 국내 거베라 18품종 중, Agrobacferium 감염에 순응적인 품종을 선발하기 위하여 18개의 국내 거베라 품종을 스크린하여 011, 016, GM023 등 12개의 순응형 계통을 스크린 하였으며 효율적인 형질전환을 위한 제균 항생제로는 cefotaxime이 선택되었다. 더 많은 캘러스 유도를 위한 전처리 배지의 호르몬 농도는 BA 2 ppm, Zeatin 2 ppm, IAA 0.2 ppm (2 x media)이 효율적이었고 재분화 배지의 호르몬 농도도 전처리 배지 호르몬의 농도와 동일하게 BA 2 ppm, Zeatin 2 ppm, IAA 0.2 ppm (2 x media)에서 효율적이었으나 GUS 발현에는 다른 차이가 없었다. 고효율 형질전환 체계 확립을 위하여 네 가지 다른 전처리 기간 처리와 두 가지 다른 Agrobacterium tumerfaciens 사용, 두 가지 다른 공배양 기간을 처리한 결과 전처리 하지 않은 엽병과 엽신을 LBA4404 Agrobacterium tumerfaciens을 사용하여 5일 간 공배양 기간을 가졌을 때와 dipping 처리하여 접종한 엽병과 엽신에서 높은 GUS 발현빈도를 나타냈다. Gerber (Gerbera hybrida) is a valuable ornamental species grown as a potted plant and cut flowers. However, genetic variability within the gerbera genus is very limited. So it is absolutely needed to introduce and widen genetic resources into gerbera lines by genetic transformation. For the purpose, 18 Korean gerbera lines were screened to establish Agrobacterium-mediated genetic transformation procedure. In an experiment to select Korean gerbera lines which are amenable to Agrobacterium-inoculation, 12 lines turned out to be positive in Agrobacterium-inoculation. More callus were produced from BA 2ppm, Zeatin 2ppm, IAA 0.2ppm in pre-culture and regeneration medium (2X media) but there was no difference in the frequency of GUS expression rate. In another experiment to find out optimal condition for highly efficient Agrobacterium-inoculation, petiole and leaf explants have been treated with four different pre-culture periods, two different co-culture periods and two different Agrobacterium strains. As a result, high GUS expression has been showed from petiole and leaf explants treated no pre-culture period with LBA4404 Agrobacterium tumerfaciens, 5 day co-culture period and dipping treatment.

갈겨니(Zacco temminki)의 진화에 관한 연구 VII.갈겨니 2 Type의 Mitochondrial DNA변이

이혜영,양서영,백상기,박창신,유성림,이성근,Lee, Hei-Yung,Yang, Suh-Yung,Paik, Sang-Gi,Park, Chang-Shin,Yu, Sung-Lim,Lee, Sung-Keun 한국통합생물학회 1988 동물학회지 Vol.31 No.3

한국산 담수 어류인 갈겨니(Zacco temminki)는 형태적으로 동일하거나 전기영동법에 의한 s-Mdh에 2type(MM과 MS type)이 있음을 (1987)이 주장하였으며 2type간 유전적 차이 정도를 분석한 결과 자매종으로 밝힌바 있다. 본 연구에서는 상기 결과를 토대로 2type의 mtDNA를 4개 집단에서 추출하여 11가지 제한 요소로 처리한 다음 fragment양상을 비교 분석하였다. 갈겨니 mtDNA의 전 genome크기는 약 16.7Kb였으며 frabment homolgy(F)에서 MS type 간 F값은 0.464,MM type간 F값은 0.762로 높은 값을 나타냈다. 또한 MM과 MS 이형간 평균 F값은 0.312(0.258-0.345범위)로 동형간보다 낮은 유사성을 나타내고 있다. 집단 또는 type간 nucleotide sequence divergence(p)를 산출한 결과 MS type 간의 P=0.128에 비해 동형간의 P값은 0.045로 매우 낮은 mtDNA sequece 변이를 나타내었다. 그러나 이형간인 2type평균 P값은 0.195(0.177-0.226범위)로 차이를 나타냈다. 이와 같은 2type간의 차이는 isozyme연구 분석 결과와 일치하고 있어 갈겨니 2type간의 종분화를 확신시키는 새로운 자료가 되었다. Mitochondrial DNAs of two Mdh allelotypes of the dark chub, Z. temmincki inhabiting in Korean fresh water, were analysed. Samples of each type were collected from four populations, and the fragment patterns for mtdNA of each type were explained from 7 of the eleven restriction enzymes with hexanucleotide recognition site. Genome size was approximately 16.7 kilobases. The highly typical mtdNA fragments of each type were discovered in digestion profiles produced by Eco RI and Pst I enzyrnes. The comparisons of restriction fragment patterns and relative digestion maps permitted the estimation of fragment homology (F) and nucleotide sequence divergence(p). Between the two identical types, sequence divergence(p) was 0.128(MS), and 0.045(MM), ; between the two different types, 0.195 (range 0.177-0.226). These result may provide a distinct difference more than the value derived from allozyrne analysis, and a powerful new molecular approach for assessing genetic-evolutionary relationship among fishes.

수중운동과 타이치운동 후 여성 골관절염 환자의 근력변화

이혜영,이은옥,송라윤,Lee, Hea-Young,Lee, Eun-Ok,Song, Rha-Yun 대한근관절건강학회 2005 근관절건강학회지 Vol.12 No.2

Arthritis is one of the most common chronic degenerative joint disease in elderly. Osteoarthritis is a widespread, slowly developing disease, with a high prevalence increasing with age in women. The large joints mostly involved by the disease are the knees. But there are no treatments available that cure the underlying process of osteoarthritis diseases. Physical exercise helps in increasing cartilage nutrition and remodeling, increases the synovial blood flow, decrease swelling, and improves muscle strength. Thus, exercise has been suggested as an important nursing strategy in osteoarthritis. Purpose: The purpose of this study were to compare muscle strength between Tai-Chi exercise and aquatic exercise for women with knee osteoarthritis. Methods: A quasi-experimental study with pretest and posttest measures was used. The study subjects were those who had been enrolled in a community health center, and agreed to participate in the study for eight weeks, signed the consent form, and obtained the physicians approval. The study dropout rates were 13.2% with the final study subjects of 17 on Tai-Chi exercise, 16 on aquatic exercise program. The collected data were analyzed using SPSS for Window (version 12.0). Independent sample t-test and paired t-test was performed to compare of muscle strength for women with osteoarthritis after 8-week Tai-Chi exercise and aquatic Exercise. Results: The homogeneity tests of demographic characteristics and study variables at the pretest data revealed no significant differences between two groups. After 8-week Tai-Chi and aquatic exercise, there was significant result in pre-post test comparison on muscle strength on Tai-Chi group, but no significant in aquatic group. There were no significant differences of knee extensor (p=.078), and hand grip(p=.118) in group comparisons on muscle strengths. But there were significant differences of knee flexor(p=.024). Conclusion: Tai-chi exercise was effective in improving knee flexor. So, it seems that Tai-chi exercise may be more suitable for aquatic exercise in osteoarthritis exercise programs. Further studies with other comparisons in physical and psycho social outcomes are necessary to confirm the more effects of exercise.

국제표준화회의참가보고 - 3GPP 제74차 TSG 기술총회

이혜영,Lee, Hye-Yeong 한국정보통신기술협회 2017 TTA저널 Vol.169 No.-

지구 관측자료 공유를 위한 메타데이터 연구

이혜영,곽승진,Lee, Hye-Young,Kwak, Seung-Jin 한국문헌정보학회 2007 한국문헌정보학회지 Vol.41 No.2

The purpose of this study is to design the metadata for sharing and efficiently using the informations of Earth Observations in Korea. Recently the GEO(Group on Earth Observations) has been starting to manage all nation's informations in a way that benefits the environment as well as humanity by taking a pulse of the plants. For that reason, we have to construct the infra-system to manage and integrate the 12 social benefit areas in Korea; disaster, health, energy, climate, water, biodiversity, agriculture, forest, ocean, space information and geographical information system(GIS). But these informations have only been managed with varied metadatas in each area of observation, and then it is difficult to integrate varied informations. In this study, we solve the problem with meta-metadata. 본 연구는 지구 관측자료 공유 및 효율적 활용을 위한 통합 메타데이터를 설계하는 것이다. 최근 정부 간 조직으로 구축된 GEO(Group on Earth Observations)는 지구전반에 걸쳐 지속적으로 관측되는 자료를 활용하여 인류의 이익을 최대 창출하기 위해 노력하고 있다. 이러한 세계적 흐름에 따라 국내에서 관측되는 지구 관측자료 즉, 재해, 보건의료, 에너지자원, 기후, 수자원, 기상 생태계/생물다양성, 농업, 산림, 해양, 우주, 국토공간정보 등 12개 분야의 관측자료를 통합 및 활용하기 위한 인프라 구축이 반드시 필요하다. 현재 지구관측자료는 관측분야별로 개별적인 메타데이터를 관리하고 있을 뿐 메타데이터를 통합관리하지 못하고 있다. 국내 지구 관측자료의 관측분야와 관측요소별 다양하고 상이한 메타데이터로 인한 통합메타데이터의 설계 어려움을 메타-메타데이터를 이용하여 개발하였다.

캠퍼스 상호대차서비스 전산화에 따른 성과 분석

이혜영,Lee, Hye-Young 한국과학기술정보연구원 과학기술정보센터 2009 Journal of Information Science Theory and Practice Vol.40 No.4

많은 도서관은 한정된 자원을 극복하고 이용자의 정보요구를 충족시키기 위하여 상호대차서비스를 활용한다. 상호대차서비스는 도서관 서비스의 소유 소장의 개념에서 공유 접근의 개념으로 변화를 말하는 것으로, 상호대차서비스 평가 및 그 필요성을 재고할 필요가 있다. K대학은 단행본 중심의 캠퍼스 상호대차서비스는 오랫동안 제공하였는데, 2003년부터 온라인서비스를 제공하는 등의 노력을 지속적으로 하였다. 본 연구는 상호대차서비스의 전산화가 서비스에 미치는 영향을 분석하기 위한 것으로 9년간의 서비스운영 실적을 비교분석하였다.시스템 발전 단계에 따라 상호대차 신청과 도서 대출 건수가 급격히 증가하는 것을 볼 수 있었다. 또한 서울캠퍼스 소속 이용자의 상호대차신청건수가 2001년 전체 도서신청건수 중 12%이었던 것이 2008년 무려 75%까지 상승한 것을 확인할 수 있었다. 상호대차 처리시간도 2004년의 17% 수준으로 떨어지는 등 많은 개선 결과를 보였다. Generally, we used to provide ILL to users for overcoming the limited resource and satisfying user's desires for information. ILL makes library service's paradigm change from ownership to sharing. K-university Library has been providing Campus inter-library loan as ILL to users for long time and continually trying to improve the service's quality. This case study is to analysis the performance of campus inter-library loan in the past 9 years. As the related automation system was developed and upgraded, the service requests have been increasing annually. Campus inter-library loan requests were 12% of total book requests in 2001, and then were 75% of total book requests in 2008. The transaction time was reduced until 17% of 2004.

우리나라 소아 물리치료사의 임상적 추론

이혜영,이인희,김경,Lee, Hye-Young,Lee, In-Hee,Kim, Kyoung 대한물리치료학회 2012 대한물리치료학회지 Vol.24 No.5

Purpose: The purpose of this qualitative study was to understand the application of clinical reasoning throughout clinical and therapeutic knowledge of clinical pediatric physical therapists under physician prescriptions. Methods: Purposive sampling techniques were used in selection of nine clinical pediatric physical therapists in South Korea. Open and semi structured interviews were conducted, and were thoroughly examined and reviewed, followed by analysis of the clinical reasoning portion. Results: Pediatric physical therapists have been influenced by information gathering, physical function and evaluation of their patients, hypothesis-orientation, and re-evaluation and self-monitoring. Novice physical therapists were more dependent on physician prescriptions and parent's hope than the result of their own evaluation and critical pathway. Middle experienced pediatric physical therapists were more dependent on rapport with children and their parents. Highly experienced pediatric physical therapists were more reliant on hypothesis-orientation and self-monitoring. As reports on clinical experience of pediatric physical therapists have accumulated, clinical reasoning of pediatric physical therapists has been influenced by their experience, such as education, clinical pathology conference, their own clinical experience and rapport with patients and their parents than physicians' prescriptions. Conclusion: The findings of the current study are generally consistent with existing research on clinical reasoning. The results of the current study may be used by educators for enhancement of clinical reasoning abilities and knowledge of students or novices as well as development of a guide for use by suitable novices or students, and could provide important information for use in physical therapy practice and research.

학령기 정상 아동의 호흡 특성과 신체 조건에 관한 상관분석

이혜영,강동연,김경,Lee, Hye Young,Kang, Dong Yeon,Kim, Kyoung 대한물리치료학회 2013 대한물리치료학회지 Vol.25 No.5

Purpose: Respiratory is an essential vital component for conservation of life in human, which is controlled by respiratory muscles and its related neuromuscular regulation. The purpose of this study is to assess lung capacity and respiratory pressure in healthy children, and to investigate relationship and predictability between respiratory pressure and other related respiratory functions. Methods: A total of 31 healthy children were recruited for this study. Demographic information and respiratory related factors were assessed in terms of body surface area (BSA), chest mobility, lung capacity, and respiratory pressure. Correlation between respiratory pressure and the rested variables was analyzed, and multiple regression using the stepwise method was performed for prediction of respiratory muscle strength, in terms of respiratory pressure as the dependent variable, and demographic and other respiratory variables as the independent variable. Results: According to the results of correlation analysis, respiratory pressure showed significant correlation with age (r=0.62, p<0.01), BSA (r=0.80, p<0.01), FVC (r=0.80, p<0.01), and FEV1 (r=0.70, p<0.01). In results of multiple regression analysis using the backward elimination method, BSA and FVC were included as significant factors of the predictable statistical model. The statistical model showed a significant explanation power of 71.8%. Conclusion: These findings suggest that respiratory pressure could be a valuable measurement tool for evaluation of respiratory function, because of significant relationship with physical characteristics and lung capacity, and that BSA and FVC could be possible predictable factors to explain the degree of respiratory pressure. These findings will provide useful information for clinical assessment and treatment in healthy children as well as those with pulmonary disease.

인산(燐酸) ESTER계(系) 접착성(接着性) 시멘트의 치수반응(齒髓反應)에 관(關)한 실험적(實驗的) 연구(硏究)

이혜영,최대균,최부병,박남수,Lee, Hye-Young,Choi, Dae-Gyun,Choi, Boo-Byung,Park, Nam-Soo 대한치과보철학회 1986 대한치과보철학회지 Vol.24 No.1

It is considered that etching solution or material itself of phosphoric ester cement will induce not a little pulpal irritation, if applied directly onto unsealed dentinal tubules. This study was designed to confirm above consideration by comparing two different conditions between $Ca(OH)_2$-based and non-$Ca(OH)_2$-based group. Posterior teeth of 15 male dogs were selected for this experiment. One experimental group was filled with cement after $Ca(OH)_2$-basing and enamel-etching, the other experimental group after enamel etching without $Ca(OH)_2$-basing. And both of two experimental groups were observed at 2 hours, 15 hours, 3 days, 1 week, 2 weeks, 4 weeks, and 6 weeks after filling. The findings reached to the following conclusions histologically. 1. In both groups, the damaged odontoblasts were atrophied and eventually disappeared. 2. In non-based group at early stage, odontoblasts were severely atrophied and defective areas were appeared between odontoblast cell layers. However, in based group, the odontoblasts were arranged slight irregularly. 3. In non-based group, a small number of undifferentiated cells below the odontoblast cell layers started to appear at 1 week after filling. However, in based group, the undifferentiated cells were appeared at 15 hours after filling. 4. In non-based group, formation of reparative dentin was not begun until late stage of experiment. However, in based group, formation of reparative dentin matrix was begun at 2 weeks after filling and very thickened reparative dentin was formed at 6 weeks after filling. 5. In odontoblast cell layers of both groups, dilated capillaries were observed. 6. Argyrophilic fibers were reticularly condensed in zone of Weil, and they were connected to the pulp tissue and dentin.

소아의 상부요로감염에서 급성기 반응지표의 유용성에 관한 연구

이혜영,이백희,Lee, Hye Yeong,Lee, Baeck Hee 대한소아감염학회 1998 Pediatric Infection and Vaccine Vol.5 No.2

목 적 : 소아의 요로감염은 성인과 달리 비특이적이고 전신적인 증상이 많아 진단이 늦어질 수 있고 요로계 이상과 발견당시 이미 신 반흔이 증명되는 경우가 많아 적절한 치료를 하지 않으면 청소년기의 신부전, 고혈압, 성장부진 등의 심각한 후유증을 남길 수 있다. 특히 신장을 침범하는 상부요로감염에서 치명적인 후유증을 남기기 때문에 요 배양검사 결과가 나오기 전 임상에서 쉽게 할 수 있는 급성기 반응 지표들과 방사선학적 검사를 통해 상부요로감염을 예측하고 신속하게 치료를 시작함으로씨 합병증을 감소시키고 환자 보호자에게 치료기간과 치료 방침 등을 설명해 줌으로써 향후 치료 및 추적 검사시 도움을 얻고자 하였다. 대상 및 방법 : 1995년 l월부터 1998년 5월까지 단국대학교 병원 소아과에 입원한 환아 56명을 대상으로 입원 당시 급성기 반응지표(CRP, ESR, WBC), 농뇨와 발열정도를 정량적으로 분석하여 방광 요관 역류 정도와 함께 신초음파 및 $^{99m}TC$-DMSA 신주사와 비교 분석하였다. 통계처리는 chi-square (x2) test를 이용하였고 P<0.05를 유의한 기준으로 삼았다. 결 과 : 1) 요로감염은 l세 미만에서 남아의 발생 빈도수가 더 높았으나 l세 이후에는 여아에서 더 높았다. 2) 급성기 반응지표인 CRP, 백혈구의 수지가 높을수록 상부요로감염을 시사하였다{P<0.05). 3) 발열이 심할수록 상부요로감염을 시사하였다(P<0.05). 4) 농뇨가 심할수록 상부요로감염을 일으킬 확률이 높았다(P<0.05). 5) 방광 요관 역류정도가 심할수록 상부요로감염을 일으킬 확률이 높았다(P<0.05). 6) $^{99m}TC$-DMSA 신주사가 상부요로감염을 예측하는데 신초음파보다 더 유용하였다. 결 론 : 급성기 반응지표들과 발열의 정도, 농뇨, $^{99m}TC$-DMSA 신주사 소견 등으로 상부요로감염을 예측할 수 있고 신속한 치료로 합병증을 감소시킬 수 있으며 보호자에게 향후 치료기간과 치료방침을 설명함으로써 좋은 유대 관계 속에서 지속적인 치료 및 추적관찰을 하는데 도움이 되리라 사료된다. Purpose : The symptom of urinary tract infection in children is non-specific and systemic compared to that of adults. So the diagnosis of UTI in children can be delayed. If UTI in children is not appropriately managed, it may he resulted in renal failure, hypertension, growth failure in adolescence because there are already documented renal scar or urinary tract abnormality in infection period. Especially upper UTI that involve renal parenchyme may be result in fatal complication. The purpose of this study is analyzing the relationship between acute reactive marker and $^{99m}TC$-DMSA renal scan in upper urinary tract infection. Methods : This study included 56 children admitted at Dankook University Hospital Pediatric Department in Jan. 1995~May. 1998. We analyzed quantatively the results of acute reactive marker(CRP, ESR, WBC), pyuria, fever and compared to those of sonographically find ing and $^{99m}TC$-DMSA renal scan. Comparison between groups were performed by the chi-square (x2) test and a p value of less than 0.05 was considered statistically significant. Results : 1) The number of boys less than 1 year of age was larger than that of girls. But the number of boys more than 1 year of age was reversed. 2) The higher me level of reactive marker (CRP, WBC), the more the probability of upper UTI. 3) The higher fever, the more the probability of upper UTI. 4) The more pyuria, the more probability of upper UTI. 5) The more higher the grade of vesicoureteral reflux, the more probability of upper UTI. 6) $^{99m}TC$-DMSA renal scan is more sensitive and more specific diagnostic tool than renal sonogram. Conclusion : The appearance of an abnormal $^{99m}TC$-DMSA renal scan is correlated with acute reactive marker (CRP, ESR, WBC), fever, pyuria. $^{99m}TC$-DMSA renal scan can be a good valuable predictor tool in upper UTI. So we can start early treatment and decrease the incidence of complication of upper urinary tract by above indicators before knowing the result of urine culture. And we can follow up the patients in more good relationships with their parents by telling them the duration of treatment and follow-up plan.