Effect of aging on expression of nitric oxide and inducible nitric oxide synthase in human gingival fibroblasts

지숙,국중기,박주철,김흥중,장현선,김종관,김병옥,Ji, Suk,Kook, Jung-Ki,Park, Joo-Cheol,Kim, Heung-Joong,Jang, Hyun-Seon,Kim, Chong-Kwan,Kim, Byung-Ock Korean Academy of Periodontology 2006 Journal of Periodontal & Implant Science Vol.36 No.2

치주질환의 진행이 나이에 의해 영향을 받는다는 사실은 알려져 있으나 노화에 따른 치주조직 세포의 기능적인 변화에 관한 사실은 많이 알려져 있지 않다. 노화에 따른 세포의 노화가 치주질환의 진행에 어떠한 여향을 끼치는가를 아는 것은 중요하다. 염증 상태에서 nitric oxide (NO)는 조직 파괴에 관여하는 인자로 작용하여 치주질환의 진행에 관여하는 것으로 알려져 있다. 따라서 이 연구는 사람의 치은에서 배양된 치은섬유아세포를 이용하여 세포의 노화에 따른 NO와 이의 합성효소인 inducible nitric oxide synthase (iNOS)의 발현을 알아봄으로써 세포의 노화가 치주질환의 진행에 끼치는 영향에 대해 알아보고자 하였다. 10세의 환자와 55세의 환자에서 각각 채취한 치은에서 배양된 세포와 10세의 환자에서 채취한 세포를 계속적인 계대배양을 통해 얻은 실험실 상 노화된 세포를 포함하여 총 3 종류의 치은섬유세포를 실험에 이용하였다. Hot phenol-water extraction을 통해 추출된 Porphyromonas, gingivalis ATCC 33277 lipopolysaccharide (LPS)와 재조합 $IFN-{\gamma}$ 를 세포에 적용시켜 Griess assay를 통해 조건화된 배지에서 NO를 측정하였다. 20세와 55세의 환자에서 채취된 치은 조직과 총 3 종류의 배양된 세포에 NOS-II 항체를 적용시켜 iNOS 단백질 발현을 관찰하였다. Total RNA를 추출하여 RT-PCR를 통해 iNOS mRNA의 발현을 분석하였다. 치은섬유아세포에서 NO는 자발적으로 발생되었고, 이러한 발현은 젊은 세포보다 노화된 세포에서 강하였다. P, gingivalis LPS와 제조합 $IFN-{\gamma}$는 치은섬유아세포에서 NO의 발현을 증가시켰고, 이러한 발현은 젊은 세포보다 노화된 세포에서 강하였다. 면역조직화학 염색에서 iNOS 단백질은 젊은 사람과 노화된 사람의 치은 조직 모두에서 치은섬유아세포와 상피의 기저층 세포와 염증세포에서 발현되었으나 노화에 따른 발현의 차이를 구별할 수는 없었다. 세포의 면역염색에서 iNOS 단백질은 노화된 세포에서 강하게 발현되었고 이러한 발현은 LPS와 $IFN-{\gamma}$ 에 의해 강화되었다. LPS와 $INF-{\gamma}$ 의 조건이 주어지지 않은 상태에서 iNOS mRNA는 젊은 세포에서보다 노화된 세포에서 강하게 발현되었다. 이러한 결과를 통해 세포의 노화가 NO와 iNOS 발현을 증가시킴으로서 치주질환의 진행에 영향을 끼칠 수 있음을 시사하였다.

일독 사실주의 소설 속의 여성의 일탈 서사 ー「한 여자」의 요코와 「에피 브리스트」의 에피를 중심으로ー

신지숙 ( Shin Ji-suk ) 한국일어일문학회 2023 일어일문학연구 Vol.124 No.-

「A Certain Woman (或る女, Aru Onna, 1919)」by Arishima Takeo (有島武郎 1878-1923) and 「Effi Briest (1894/95, 1896)」by Theodor Fontane (1819-1898) are representative realist novels of Japan and Germany, respectively. This analysis provides a comparison of these two works, with focuses on the narratives of deviation and adultery of the two heroines: Yōko Satsuki and Effi. These two women have something in common. First, they both rebel against the virtues of a conventional society which values patriarchy and honor, especially in an era in which gender discrimination becomes more evident as a “social construct” as the family is incorporated into society and the state. Secondly, in the pain that they feel as punishment, neither acknowledgment nor healing of their sins takes place inside the two heroines. While Yoko is protesting to God in pain that she interprets as a punishment from God, Effi attempted to develop a sense of guilt in the painful situation that she tried to interpret as punishment, but in vain. Inside her, only hatred for Instetten and society amplified. However, Effi got healed and grew into a true loving person, in the consolation of nature and in the acceptance and love of her parents, which came after her suffering. On the other hand, the obvious difference between the two heroines is the attitude of taking responsibility for their own actions. While Effi, who got healed, admits her fault of betrayal, Yoko, who has not experienced healing, refuses to accept the responsibility and protests to the God who rules her fate, despite admitting the wrongs she has done in her life. In her protest, the author Arishima’s doubts about the Christian theory of atonement are projected.

시간적 추이에 따른 19세 이상 한국 성인 당뇨병 환자의 유병률과 관련 요인

성지숙(Ji-Suk Seong) 한국컴퓨터정보학회 2024 한국컴퓨터정보학회논문지 Vol.29 No.8

The purpose of this study is to identify the prevalence of diabetes and related factors and thereby to provide fundamental data for the prevention and management of diabetes. This study is a secondary analysis based on the raw data from the Korea National Health and Nutrition Examination Survey (KNHANES) for the years 2011 and 2021. Analysis method was frequency analysis, complex samples Chi-square test, complex samples logistic regression analysis. As a result of the study, the factors related to diabetes were sex, age, education level, hypertension, dyslipidemia, and subjective health recognition in 2011(Negelkerke R²=.255), and sex, age, education level, hypertension, dyslipidemia, mother’s diabetes, and subjective health recognition in 2021(Negelkerke R2=.327). This study is meaningful in that the factors related to diabetes were identified according to time trend, and the changes in the related factors were confirmed. Development and application of an intervention program to prevent diabetes are necessary.

스마트폰 사용으로 인한 손상경험에 영향을 미치는 요인

홍지숙(Ji-Suk Hong),이종화(Jong-Hwa Lee) 인문사회과학기술융합학회 2018 예술인문사회융합멀티미디어논문지 Vol.8 No.7

본 연구는 일반청소년과 다문화청소년의 스마트폰 사용경험에 따른 신체손상 경험 수준을 알아보고, 스마트폰 사용경험에 미치는 영향력을 분석하였다. 이를 위해 보건복지부의 제13차(2017년) 청소년건강행태온라인조사 빅데이터를 이용하였으며, 참여학생은 중․고등학교 799개교, 62,276명이었다. 분석결과, 스마트폰 사용으로 손상경험에 미치는 영향을 비교한 결과, 일반청소년은 학년, 가정경제상태, 거주형태, 학업성적, 음주경험, 흡연경험, 약물경험 등이 영향을 미치는 것으로 나타났다. 다문화청소년은 학년, 가정경제상태, 학업성적 등이 영향을 미치는 것으로 나타났다. 따라서 이러한 연구결과를 바탕으로 정책적 측면에서 스마트폰이 미치는 부정적 영향에 대한 정책적 대안을 마련하는 것이 필요하며, 실천적 측면에서는 스마트폰 사용에 따른 청소년의 스마트폰 사용경험에 따른 손상의 위험성을 크게 낮출 수 있도록 다각적인 예방 프로그램과 대응적인 프로그램을 적극적으로 개발할 필요가 있다. This article investigated the level of body damage according to Smartphone usage for the general youth and the multicultural youth and analyzed the effect on Smartphone usage. For this, we have used the 13th Korea Youth Risk Behavior Web-based Survey 2017 survey big data of the Ministry of Health and Welfare, and 62,276 students of 799 middle and high schools were participated in it. As the result, there was the effect of grade, domestic economy, residential types, scholastic performance, drinking, smoking and drug on Smartphone usage for the general youth. On the other hand, there was the effect of grade, domestic economy and scholastic performance on Smartphone usage for the multicultural youth. Therefore, it is necessary to prepare policy alternatives for the negative effects of smart phones on the basis of these research results. In practical terms, it is necessary to reduce the risk of damage due to the experience of using smartphone It is necessary to actively develop various preventive programs and corresponding programs.

초등 과학영재와 일반학생이 지각하는 부모의 양육태도가 학업탄력성에 미치는 영향

노지숙 ( Noh Ji Suk ),여상인 ( Yeo Sang Ihn ) 서울교육대학교 초등교육연구원 2016 한국초등교육 Vol.27 No.3

This study aims to investigate the effects of parenting attitude perceived by the science-gifted and non-gifted elementary students on their academic resilience. The subjects of this study were total 257 6th grade elementary school students composed of 65 students in institutions for education of gifted students, 93 students in the classes for gifted students, 99 students in the classes for normal students in the same school district. In order to investigate the difference between academic resilience and parenting attitude perceived by students, ANOVA and Scheffe test were done. In order to find out about the influence of parenting attitude on academic resilience, regression analysis was used. The results of this study were as follows: First, there were statistically significant differences in parenting attitude perceived by science-gifted and non-gifted elementary students , and there were statistically significant differences among the three groups in the difference of academic resilience between science-gifted and non-gifted students. Second, explanation power of the parenting attitude on the academic resilience appeared as 31.8~47.5%.

여성소비자의 라이프스타일과 레저활동에 따른 아웃도어웨어 구매행동

안지숙(Ji-Suk Ahn),정재철(Jae-Chul Jeong),박명자(Myung-Ja Park) 한국의상디자인학회 2018 한국의상디자인학회지 Vol.20 No.3

소셜미디어 빅데이터를 활용한 항공사 이미지 변화 분석

홍지숙(Ji-suk Hong),오익근(Ick-keun Oh) 한국관광연구학회 2016 관광연구저널 Vol.30 No.6

To survive keen competition, its necessary for corporations to build good corporate images for consumers. The purpose of this study is to suggest some implications to improve and enhance corporate image after a negative incident. This study is focused on the image difference through big data analysis of “K” airline before and after a ramp return. The big data were collected by text mining, co-occurrence frequency analysis, and semantic network analysis and then analyzed. The results are as follows. First, the amount of data increased steeply after the incident, which could significantly affect public image formation or recognition. Second, most of the data about “K” airline were related to flight routes and schedules, cabin crews, and recruiting before the incident. The airline was presented as a socially responsible and ethical corporation. On the other hand, after the incident most of the data collected contained words displaying a negative representation of executives. Third, through ego network analysis, central images of people associated with the airline before the incident were crew, but after they were executives who were related to the crisis. The results show that after the incident, the data presented negative images about “K” airline. More than anything, in the companys crisis management communications the management of people associated with the incident is important. Finally, some implications for crisis management are provided.

임상사례연구: 표현예술심리치료에 참여한 자폐아동의 경험

노지숙 ( Ro Ji Suk ) 대한무용동작심리치료학회 2018 신체심리연구 Vol.4 No.1

본 연구는 자폐 아동의 자기표현이 표현예술심리치료 과정에서 자폐아동이 어떤 변화를 경험하는지 심층적으로 알아보고자 하는 것을 목적으로 한다. 연구 참여 아동은 2급 자폐진단을 받은 만 9세 자폐아동이며 2018년 2월 ~ 2018년 9월까지 8개월 동안 23회기 표현예술심리치료를 진행한 단일사례에 대한 연구이다. 이 기간 동안 연구자는 표현예술심리치료사로서 자폐 아동의 표현을 음악, 움직임, 언어, 미술 등의 매체를 통해 다양하게 공감하고 수용하는 치료 환경을 제공하였다. 본 사례 연구는 각 세션에 관한 주관적인 기술을 통해 표현예술심리치료에서 자폐 아동이 어떤 경험을 하는지 이해할 수 있도록 하는 데 초점을 두었다. 연구 결과 자폐 아동은 표현예술심리치료 안에서 상동행동이 변화, 신체적 발달의 이행, 자아 응집의 변화가 관찰되었다. 이런 결과로 부터 표현예술심리치료가 자폐 등 장애를 앓고 있는 아동들의 자기표현과 아동의 심리, 신체 발달, 자아감 형성과 관련된 경험을 제공하고 있다는 것을 알 수 있었다. The purpose of this research is to address how autistic child's self-expression experiences changed in the process of expressive art psychotherapy. This research is a single case research of expressive art psychotherapy which proceeded for 8 months, 23 sessions from February 2018 to September 2018, and child who participated in this research is nine-years-old, diagnosed as second graded autism. During this period, researcher, as expressive art psychotherapist, provided a treatment environment which can empathize and embrace autistic child's expression through media such as music, movement, language and art. This case research focused on leading viewier to understand what the autistic child going through during the each session of expressive art psychotherapy with detailed description. In the result of this research, autistic child showed change of their stereotyped-behavior, progress on physical development, and strengthened self-awareness in expressive art psychotherapy. From this result, the researcher came to understand that the expressive art psychotherapy has impact on self expression, mental state, physical development, formation of self-awareness of children who has disability such as autism.

실험 설계에서 나타난 소집단 논변활동 탐색: 활동에 대한 인식적 목표와 인식적 이해를 중심으로

권지숙 ( Ji Suk Kwon ),김희백 ( Heui Baik Kim ) 한국과학교육학회 2016 한국과학교육학회지 Vol.36 No.1

The purpose of this study is to explore students’ epistemic goals and considerations in designing an experiment task and to investigate how a shift in the students’ epistemology affected their argumentation. Four 7th grade students were selected as a focus group. According to the results, when they designed their own experiment, their epistemic goal was ‘scientific sense-making’ and their epistemic considerations. the perception of the nature of the knowledge product was ‘this experiment should explain how something happened’, the perception of the justification was ‘we need to use our interpretation of the data’ and the perception of the audience was ‘constructor’. contributed to designing their experiment actively. When students tried to select one argument, their epistemic goal shifted to ‘winning a debate’, showing ‘my experiment is better than the others’ with the perception of the audience, ‘competitor’. Consequently, students only deprecated the limits of different experiment so that they did not explore the meaning of each experiment design deeply. Eventually, student A’s experiment design was selected due to time restrictions. When they elaborated upon their result, their epistemic goal shifted to ‘scientific sensemaking’, reviewing ‘how this experiment design is scientifically valid’ through scientific justification. we need justification to make members accept it . acting as ‘cooperator’. Consequently, all members engaged in a productive argumentation that led to the development of the group result. This study lays the foundation for future work on understanding students’ epistemic goals and considerations to prompt productive argumentation in science classrooms.

수직 치근 파절의 임상적 및 방사선적 연구

지숙 ( Suk Ji ),정숭우 ( Seung U Jeong ),황규영 ( Kyu Young Huang ) 조선대학교 치의학연구원 2003 Oral Biology Research (Oral Biol Res) Vol.27 No.1