전해수 생성온도에 따른 그람양성균과 그람음성균의 살균 효과

이정민(Jeong Min Lee),정현정(Hyun-Jung Chung),방우석(Woo Suk Bang) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.8

본 연구에서는 다양한 온도의 물로 제조한 전해수를 이용하여 그람양성균과 그람음성균에 대한 살균력을 확인하였다. 전해수의 물성은 물의 온도가 높아질수록 pH와 유효 잔류염소 농도 값이 높아졌으나, 산화환원전위 값은 감소하였다. 4, 22, 40°C의 물을 이용하여 생성한 전해수에 Escherichia coli O157:H7을 15분 동안 처리하였을 때 각각 1.18, 4.47, 5.46 log CFU/mL 감소하였으며, Staphylococcus aureus는 각각 0.72, 4.90, 5.54 log CFU/mL 감소하였다. 이를 통해 전해수를 생성하는 물의 온도가 증가할수록 살균 효과가 높아지는 것을 알 수 있다. 4, 40°C의 물을 이용하여 생성한 전해수에 균주를 처리한 경우, Listeria monocytogenes의 D 값(일정한 온도에서 90 %의 미생물이 사멸하는 데 걸리는 시간)이 각각 6.60, 1.57분으로 나타나 가장 낮았으며, 22°C 전해수에서는 Salmonella Enteritidis의 D 값이 2.92분으로 가장 낮은 값을 나타내었다. 그람양성균과 그람음성균을 비교하였을 때 모든 온도에서 D 값에 대한 유의적인 차이는 나타나지 않았다(P>0.05). 본 연구 결과는 전해수를 제조할 경우 높은 온도의 물을 이용하여 제조하는 것이 미생물 살균 효과가 높다는 것을 보여주고 있다. 따라서 전해수를 생성할 때 물의 온도를 고려하여 높은 살균력을 나타내는 전해수를 생성할 수 있으며, 이는 물의 온도 외에도 물의 경도나 물의 종류 등을 고려하여 최적의 살균 전해수를 제조함으로써 식품산업에 적용하기 위한 자료로 활용될 것으로 기대된다. Electrolyzed activated water (EAW) has been reported to exhibit strong bactericidal effects on foodborne microorganisms. However, the disinfection efficacy of EAW is affected by factors such as water source and hardness. This study investigated bactericidal effects of EAW against three gram-positive (Bacillus cereus, Listeria monocytogenes, and Staphylococcus aureus) and three gram-negative (Cronobacter sakazakii, Escherichia coli O157:H7, and Salmonella Enteritidis) foodborne pathogens. Six strains were treated with EAW prepared at different water temperatures (4, 22, and 40°C) for 15 min, and D-values were generated. The results show that the lowest D-values for Lis. monocytogenes by EAW produced at 4°C and 40°C were 6.60 and 1.57 min, respectively. The lowest D-value for Sal. Enteritidis by EAW produced at 22°C was 2.92 min. D-values of all strains treated by EAW produced at 40°C decreased significantly compared to those treated by EAW produced at 4°C (P<0.05). These results demonstrate that applying EAW produced at warm temperature is more effective for reducing foodborne pathogens for food safety.

잎담배 중 유리 아미노산 분석

이정민,민혜정,장기철,황건중,Lee, Jeong-Min,Min, Hey-Jung,Jang, Gi-Chul,Hwang, Keon-Joong 한국연초학회 2010 한국연초학회지 Vol.32 No.2

A simple and sensitive automatic amino acid analyzer method for the determination of free amino acids in tobacco was described. Sample preparation consisted of a single step of extraction with 0.1 mol HCl at ambient temperature in 60 min by sonication, followed by filtration of an aliquot. Automated amino acid analyzer was used to construct a post-column ninhydrin reaction unit to monitor amino acids separated by liquid chromatography using a series of eluting buffers. By optimization of sample preparation, separation of 19 amino acids was achieved. Limits of quantitation was 0.01 mg/g, coefficients of variation ranged from 0.5 % to 8.9 % and recoveries range from 85 % to 106 %. The method was applied to the analysis of amino acids contents of tobacco leaves in different varieties.

피프로닐 오염도 조사를 위한 분석법 정립

조순길 ( Soon-kil Cho ),이미진 ( Mi-jin Lee ),이정민 ( Jeong-min Lee ),김호진 ( Ho-jin Kim ),박수민 ( Soo-min Park ),박혜진 ( Hye-jin Park ),이승화 ( Seung-hwa Lee ),안종성 ( Jong-sung Ahn ) 한국환경농학회 2018 한국환경농학회 학술대회집 Vol.2018 No.-

2017년 유럽에서 피프로닐에 오염된 계란이 유통된데 이어 국내에서 생산된 일부 계란에서도 살충제가 검출되었다. 피프로닐은 진드기 방제 목적으로 사용되는 살충제로 sulfide, sulfone, amide, sulfoxide의 대사산물을 생성한다. 피프로닐은 물에 1.9 mg/L 정도 용해되고, 아세톤에 545.9g/L가 용해되는 성질이 있어 시료 채취시 아세톤을 사용한다. 농장시설 등에 부착된 피프로닐을 제거하기 위해 5% 탄산나트륨(Na2CO3)과 15% 과산화수소(H2O2)를 권장하고 있다. 이는 피프로닐을 피프로닐설폰으로 산화시킨 다음 반복적으로 사용하여 제거한다. 이러한 제거 작업을 통해 피프로닐이 제거된 정도를 조사할 필요가 있고, 이를 위한 분석법 정립이 필요하다. 시료를 250 mL 용기에 넣고 1% 함유 아세토니트릴 100 mL을 주가하여 10분간 격렬하게 진탕한 후, 3500 rpm으로 원심분리를 하였다. 추출액 1 mL을 0.2 um 멤브레인필터로 여과하여 시험용액으로 하였다. 칼럼은 C18(2.0 mm I.d×50mm, 3 um)을 사용하였고, 이동상은 0.1% 포름산 함유 메탄올과 0.1% 포름산 및 5 mM 아세트산암모늄이 함유된 물을 사용하였다. 분석조건으로 피프로닐의 선구이온은 437 m/z, 생성이온은 368, 255, 290 m/z, positive 조건으로 분석하였고, 피프로닐 설폰의 선구이온은 451 m/z, 생성이온은 415, 282, 244 m/z negative 조건으로 분석하였다. 분석법의 정량한계는 피프로닐 0.92 ug/kg, 피프로닐설폰 0.70 ug/kg이었다. 피프로닐과 피프로닐 설폰을 각각 1, 5, 10, 50, 100 ug/kg 수준으로 검량선을 작성한 결과, r2는 모두 0.9999이었다. 10 ug/kg과 50 ug/kg 수준의 3반복 회수율 실험결과, 10ug/kg 수준에서 피프로닐은 74.7±0.56%, 피프로닐 설폰은 88.9± 0.27%로 나타났다. 50 ug/kg 수준에서는 피프로닐은 78.6±0.11%, 피프로닐 설폰은 95.2±0.02%로 유효회수율 범위(70~120%)에 포함되었다. 머무름 시간은 피프로닐 5.3 mim, 피프로닐 설폰은 5.7min에서 검출되었다. 교차확인 목적으로 LC/MS/MS로 분석한 시료를 GC/μ-ECD로 분석을 하였다. 분석조건은 주입구 온도 250℃, (5%-Phenyl)-methylpolysiloxane이 충진된 30 m×0.25 mm×0.25 um길이의 칼럼이다. 오븐온도는 130℃에서 300℃까지 승온 조건으로 분석하였고, 머무름시간은 피프로닐 11.64 min, 피프로닐 설폰12.79 min으로 LC/MS/MS와 GC/μ-ECD 모두 표준물질과 시료의 결과가 일치함을 알 수 있었다. 정립된 분석법은 피프로닐 오염도조사를 위한 분석법으로 활용할 수 있을 것으로 판단된다.

기능성 족지장단분석과 X-ray의 골반변위 진단의 비교 연구 - X-ray 촬영 자세 및 평가 기준에 따라 -

이정민,국길호,최보미,정현아,홍서영,Lee, Jung-Min,Koog, Gil-Ho,Choi, Bo-Mi,Jeong, Hyun-A,Hong, Seo-Young 척추신경추나의학회 2010 척추신경추나의학회지 Vol.5 No.2

Objectives: This study was carried out to investigate the relationship of leg length analysis and X-ray finding according to positions and valuation bases on diagnosis of pelvic malpositions. Methods: Twenty-two people who get $33.09{\pm}10.73$ as average were evaluated by leg length analysis and X-ray findings. After measuring innominate measurement(IM), femur head line. distance between S2 and posterior superior iliac spine(PSIS). ilium shadow measurement(ISM), major axis of obturator foramen(MaF), minor axis of obturator foramen(MiF) and distance between off centering measurement and symphysis pubis, those were analyzed. Results: 1. In assessment of posterior rotation malposition ilium(PI), it was showed the best coincidence between leg length analysis and X-ray analyzed by 1M in supine position(11 cases, 50.5%). 2. In assessment of inflare, coincidence index between leg length analysis and X-ray were not good($4.00{\pm}3.03$ cases, $18.15{\pm}13.82%$). And leg length analysis were not sensitive. 3. On the whole, coincidence index between leg length analysis and X-ray were not good(best: 1 case, 45.5%; worst: 11 cases, 50.0%). Conclusions: Results form this investigation showed the relationship of leg length analysis and X-ray according 10 positions and valuation bases on diagnosis of pelvic malpositions. This results are expected to contribute to establish method of assessment in diagnosis of pelvic malpositions.

은나노와이어와 2차원 구조 루테늄산화물 하이브리드 재료의 광전기적 특성

이정민,박희정,Jeong Min Lee,Hee Jung Park 한국결정성장학회 2024 한국결정성장학회지 Vol.34 No.2

Two-dimensional (2D) RuO₂ nanosheets with nanometer thickness were synthesized using a chemical exfoliation method. The synthesized 2D-RuO₂ was hybridized with Ag-nanowire (NW), which is attracting attention as a next-generation transparent electrode material. After coating Ag-NW on the substrate, 2D-RuO₂ was subsequently coated on the Ag-NW. Although there was a decrease in optical transmittance, the hybridization of 2D-RuO₂ confirmed the effect of reducing sheet resistance. Furthermore, the flexibility of the fabricated transparent electrodes was also studied. It was confirmed by the change in sheet resistance after bending. The additional coating of 2D-RuO₂ improved the flexibility of the transparent electrodes.

GC/MS를 이용한 잎담배 중 알칼로이드 함량 분석

이정민,민혜정,김용하,이문수,Lee Jeong-Min,Min Hye-Jung,Kim Yong-Ha,Rhee Moon-Soo 한국연초학회 2005 한국연초학회지 Vol.27 No.1

To obtain the optimum condition for analysis of 10 alkaloids in tobacco leaves, such as nicotine, nornicotine, anatabine, anabasine, myosmine, cotinine, 2,3'-dipyridyl, $\beta-nicotyrine,\;\beta-nornicotyrine\;and\;\beta-formylnornicotin$, 5 types of extraction method were investigated by GC-FID and GC/MS. The optimum condition of alkaloid extraction was achieved by using methanol:dichloromethane(1:3, v/v) after NaOH treatment. The use of mass selective detector (MSD) provided unambiguous nicotine related alkaloid analysis. Alkaloids in various tobacco leaves were extracted with the optimum extraction condition and quantified by GC/MS/SIM mode. Compared with concentrations of alkaloids among the various tobacco leaves, the concentration of alkaloids was generally in the order burley > flue-cured > oriental tobacco. In flue-cured tobacco leaves, the order of concentration of alkaloids was nicotine > anatabine > nornicotine > $\beta-nicotyrine\;>\;\beta-formylnornicotine\; >\;myosmine\;>\;2,3'-dipyridyl\;>\;cotinine\;>\;anabasine\;>\;\beta-nornicotyrine$. However, in the case of burley and oriental tobacco leaves, the concentration of nornicotine was higher than that of anatabine.

출판저널 칼럼

이정민,Lee, Jeong-Min 대한출판문화협회 1996 출판저널 Vol.188 No.-

부동산 건설업의 원가구조 변화에 대응한 공종별 신활동기준 원가관리 기법에 관한 연구

이정민,Lee Jeong-Min 한국건설관리학회 2003 건설관리 : 한국건설관리학회 학회지 Vol.4 No.4

국내 부동산 건설기업 중 2001년 말 현재 등록된 업체 중 약 93$\%$가 5억원 이하(적자 기업 포함)의 당기순이 익을 나타내고 있는 실정이다. 과거 10여년간의 매출원가 율과 매출원가 구성비율을 분석한 결과 매출원가 구성비에 큰 변화가 있었음을 발견하였다. 재료비, 노무비는 점차 감소하였고 외주가공비는 크게 증가하였다. 원가통제의 기본이 되는 활동점을 찾기 위해 공종별 신활동기준 원가관리를 모색하였다 부동산 건설업의 특징이 수주금액(판매금액)이 확정되어 있고 원가사용에 따라 이익 규모가 달라진다. 확정된 수주금액에서 최대의 이익을 창출하고, 새로운 원가관리 방법을 찾아 지속적인 경영혁신을 이루어야 할 시점이다. 공종별 신활동기준 원가관리는 부동산 건설업의 생존경영을 위한 관리혁신으로 생각된다. 실행예산과 경영원가 통제는 같은 대상을 놓고 서로 다른 관점에서 일정과 비용을 통제한다. 실행예산은 구체적인 활동 중심으로 집행되고, 경영원가는 지출내역인 재료비, 노무비, 외주비, 경비의 형태로 통제된다. 공종별 신활동기준 원가관리 방법을 이용하여 실행하기 위해서는 우선 활동의 동인이 무엇인가? 그 활동이 얼마만큼의 부가가치 창출을 하는지 사전에 분석되어 실행해야 한다. 부동산 건설업의 공종별 신활동기준 원가관리 기법은 부동산 건설업의 생존경영에 꼭 필요한 원가 관리 방법으로 지속적인 연구가 필요하다고 생각된다. About 93$\%$ of domestic teal estate construction firms registered as the end of 2001 recorded net profits of only less than 500 million won(including firms in the red) for the term. As a result of having analyzed the ratios of sales costs and the structural ratios of sales costs for the past 10 years, it was found that there have been great changes in structural ratios of sales costs. Material costs and labor costs have gradually decreased, but outsourcing costs of processing have greatly increased. In order to find activity points which are fundamental to cost control, the methods of new activity based cost management have been pursued. The characteristics of real estate construction industry lie in the fact that contract prices (sales in) are fixed and amounts of profits differ depending on the use of costs. In order to create maximum profits from fixed contract prices, the new activity based cost management has been proposed. The control of operation budgets and management costs is designed to control their schedules and expenses in different respects. Operation budgets ate executed with specific activities and management costs are controlled as a form of material costs, labor costs, out sourcing costs and expenses which are details of expenditure. In order to execute them by using the methods of new activity based cost management, first of all, we have to analyze what activity drivers ale and how much added values such activities can create. It is considered as a method of cost management which is necessary far the survival management of real estate construction industry.

간헐외사시 수술직후 발생한 과교정의 자연 회복 양상

이정민,신선영.Jeong-Min Lee. M.D.. Sun-Young Shin. M.D. 대한안과학회 2005 대한안과학회지 Vol.46 No.5

Purpose: To assess the factors which are related to recovery time from overcorrection that occurred immediately after surgery. Methods: We reviewed the medical records of intermittent exotropia patients who had undergone bilateral lateral rectus muscle recession from February 2002 to July 2004. Fifty patients who developed overcorrection of less than 20 PD on the immediate postoperative day were included in this study. Age at surgery, sex, refractive power, preoperative deviation angle, Worth 4-dot test, and stereopsis were examined, and we evaluated the correlation between these factors and recovery time. Results: Thirty-six patients (72%) recovered to orthoposition within 4 weeks postoperatively. Significant correlations were found between the recovery time and poor stereopsis (p=0.02), and suppression of the Worth 4-dot test (p=0.01). There was no significant relationship between the recovery time and age at surgery, sex, refractive power, and preoperative deviation angle. Conclusions: In cases of immediate postoperative overcorrection of less than 20 PD after intermittent exotropia surgery, factors related to delayed recovery were poor stereopsis and suppression of the Worth 4-dot test.

버어리종 담배 중 질산성 질소에 대한 측정불확도

이정민,이경구,한상빈,Lee Jeong-Min,Lee Kyoung-Ku,Han Sang-Bin 한국연초학회 2005 한국연초학회지 Vol.27 No.2

The uncertainty of measurement in nitrate from burley leaf tobacco by continuous-flow analysis method was evaluated following internationally accepted guidelines. The sources of uncertainty associated with the analysis of nitrate were weight of standard and sample, purity of standard, dilution of standard solution, calibration curve, water content, etc. The calculation of uncertainty based on the GUM(Guide to the Expression of Uncertainty in Measurement) and EURACHEM/CITAC Guide. An expanded uncertainty was obtained by multiplying the combined standard uncertainty with a coverage factor (k) calculated from the effective degree of freedom. The concentration of nitrate from burley leaf tobacco was $2.09\%$ and the expanded uncertainty by multiplying by the coverage factor(k, 2.20) was $0.13\%\;at\;a\;95\%$ confidence level.