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      • KCI등재

        소나무 자엽을 이용한 원형질체 나출(裸出) 및 배양

        이석구,윤양,이재순,이경준 한국임학회 1985 한국산림과학회지 Vol.70 No.1

        針葉樹類의 原形質體 培養可能性을 試驗하기 위하여, 소나무 種子의 胚子葉에서 原形質體를 裸出하여 培養을 試圖하였다. 9日동안 發芽시킨 採種園産 소나무 種子에서 길이가 3∼4㎜가량 되는 胚의 子葉을 잘라내어 Cellulase Onozuka R-10과 Macerozyme을 添加한 酵素 容液에서 8時間 培養하여, 活性이 높은 原形質體를 大量으로 얻을 수 있었다. 裸出된 原形質體를 B_5 培地의 無機鹽, 비타민, 아미노산, 有機酸, 糖類, 植物 홀몬이 들어있는 培地에 培養하였을 때, 12時間 經過後 첫 budding이 觀察되었으며, 3∼4日 經過後 3∼4個의 細胞로 分裂하였다. 細胞分裂은 赤道板을 가운데 둔 二等分式 分裂이 아니고, 대부분 酵母菌과 같이 budding에 依한 分裂이었다. 細胞分裂은 4個의 細胞狀態에서 中斷되어 더 이상 進展되지 않았다. 소나무 種子를 17日 혹은 24日間 發芽시켜서 完全히 자란 子葉을 使用했을 때에는 原形質體 裸出은 쉽게 이루어졌으나, 培養時에 細胞分裂이 이루어지지 않았다.

      • 압전형 구동기를 갖는 이동기구의 운동해석 및 제어

        이석구,지원호,이종원 한국소음진동공학회 1992 소음 진동 Vol.2 No.1

        The motion analysis of a translation device driven by a piezoelectric actuator is performed to identify the mechanics of impact drive mechanism and to find the maximum speed waveform. The translation device is modeled as a semidefinite two-degree-of-freedom system. The motion analysis includes effects of friction force between moving mass and contact surface, dynamics of voltage amplifier and piezoelectric elements, and hysteresis of piezoelectric actuator. Base on the model, simulation studies are carried out and then compared with experimental results. It is found that the error between moving distances obtained by analysis and experiment is less than 15% and that the actual motion of moving mass is well predicted by the analytical work, finally, precision positioning experiments are carried out by using a proximity sensor as a feedback sensor. Position control of moving mass is initiated by the maximum speed waveform and finely tuned by the scaled down waveform so that accurate positioning is accomplished within the resolution of the sensor.

      • KCI등재후보
      • KCI등재

        죄의 알레고리인가, 알레고리의 죄인가? —쿳시의 『치욕』과 재현의 정치학

        이석구 한국현대영미소설학회 2009 현대영미소설 Vol.16 No.3

        J. M. Coetzee's Disgrace is no less controversial than his previous works for its usual vague politics and employment of problematic racial/gender discourses. The novel evolves around two sexual incidents: One is David Lurie's relationship with Melanic Isaacs, a colored student of his, and the other is three blacks' violation of Lucy, Lurie's daughter. Lurie finds himself facing a sexual harassment charge as a consequence of a sexual relationship with his Romantics student. At an inquiry, Lurie, without hesitation, pleads guilty as charged but refuses to offer an apology or show any sign of repentance. This puzzling behavior of Lurie's is understood by not a few critics as allusive to those of the white perpetrators of racial discrimination at the hearings of the Truth and Reconciliation Commission. As puzzling as Lurie's bearing at the inquiry is Lucy's dealing with the gang rape and its consequences. Quite a few researchers read Lucy's enigmatic behaviors in a racial context, that is, as an allegory of the price the whites are expected to pay for continuous residency in a new post-apartheid South Africa. Unlike the second sexual incident, the significances of the first incident, this paper argues, are yet to be accounted for. Thus, by specifically probing into the allegorical dimension to the first sexual incident, this paper answers such questions as why the male protagonist forms an all-risking scandalous relationship with his student, and why he refuses to issue any public apology despite his immediate acceptance of guilt. The paper argues that the enigma can be explained when viewed in the political context of a specific political/ethnic group, that is, the liberal white South Africans. In other words, the politically and morally ambiguous situation in which the liberal whites found themselves during the era of apartheid, is behind Lurie's self-contradictory acts of pleading guilty, yet refusing to repent, and also of violating the professional ethics code, yet at times defending himself in the name of individual rights. The conclusion of this paper is that while, by encoding the South African racial relations in sexual terms, Coetzee was able to add another significant and sophisticated allegorical dimension to his crime-and-punishment-like narrative, this achievement entailed a liability for violating the sexual Other in the realm of representation.

      • KCI등재

        연속증식을 위한 기내 배양법

        이석구,이봉춘,김재헌,박재인,현영일,변량선 한국임학회 1989 한국산림과학회지 Vol.78 No.1

        지금까지 micro-propagation을 爲한 器內培養法은 試驗官이나 培養甁에 agar media를 넣어 axillary bud 또는 shoot를 置床하여 multiple shoot를 誘導 生長시킨 後, 1個月 마다 새로운 media의 器內에 옮겨주는 培養法을 利用하고 있으나, subculture 代身에 液體培地를 注入하고 있으나, subculture 代身에 液體培地를 注入하고 shoot를 繼續 生長시켜 生産하는 器內採穗圃의 役割을 할 수 있는 培養法을 試圖하였다. 상수리와 박달나무를 材料로 WPM 基本培地를 使用하였다. 培養甁의 agar media에 처음에는 培養 1個月後부터 液體培地를 1週 間隔으로 注入, 하루밤 동안 laminar air flow hood內에 두었다가 眞空펌프를 利用하여 液體培地를 除去하고 培養하며, 培養甁에서 자란 shoot를 잘라난 後에도 새로운 줄기의 發生이 繼續 이루어졌다. 또한 agar media 代身에 shoot나 axillary bud를 支持할 수 있는 stainless 鐵鋼을 利用하면, 液體培地 狀態로 樹種에 따라 培養이 可能하므로, 이에 對한 結果를 報告하고저 한다.

      • KCI등재

        쿳시의 『야만인을 기다리며』에 나타난 자유주의와 알레고리 문제

        이석구 한국비교문학회 2008 比較文學 Vol.0 No.46

        Although many Western scholars regarded the protagonists of Coetzee's works as champions of Western liberalism, this study asserts that the magistrate of The Waiting for the Barbarians is far from a liberal hero, i.e., the kind of figure who tries to uphold liberal principles and also reaches a great moral awakening through a painful process of physical and spiritual mortification. For instance, the magistrate believes himself to and also tries to be morally differentiable from the Empire. However, what he is not aware of is that he sees through the eyes of the Empire even after he openly rebels against the Empire. Even his attempt at purifying himself of the sin of his colleague, is not free from a pursuit of self-interest. Due to his failure to see a nomadic girl as an equal human being, the magistrate nullifies the possibility for a genuine interracial relationship. Viewed in this context, the magistrate, this study argues, is comparable to the English-speaking white in South Africa. This argument is based on the fact that the English-speakers in South Africa were denounced by the radical blacks as feeding on the very system they criticized and also the fact that any possible alliance with them was flatly denied by the leaders of the Black Consciousness Movement of 1970's. This movement's view that "Even the best-intentioned white could never really understand the suffering of the blacks" is alluded to when the magistrate, even with his humanitarian attitude, fails to understand what the "barbarian girl" really wanted from him. Coetzee's novels are often allegorical, and this narrative feature that tends to solicit abstract and universal interpretation has brought upon the author an ignominy of historical escapism. This study is an attempt to write back to the negative criticism by interpreting Coetzee's novel within the context of South Africa, specifically, of the dynamics between the South African English-speakers and blacks.

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