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극동산 뱀장어(Anguilla japonica)의 친자확인을 위한 유전자 마커 개발
노은수,신은하,박경현,김은미,김영옥,유용운,김신권,남보혜 한국수산과학회 2022 한국수산과학회지 Vol.55 No.5
The Japanese eel Anguilla japonica is a highly valued research object that is important for aquaculture in Asia, including the Republic of Korea. However, few studies have been conducted analyzing parentage using microsatellite markers derived from the Japanese eel. We acquired Japanese eel genome data using next generation sequencing technology, and constructed a draft genome comprising 1,087 Mbp. Using the Simple Sequence Repeat Identification Tool program, 444,724 microsatellites were identified. Of these, 1,842 microsatellites located in the 3' untranslated region, which are stably inherited, were finally selected. Ninety-six primers were selected to validate polymorphism at these microsatellites, and 9 primers were finally identified for multiplex analysis. Using multiplex polymerase chain reaction with three different fluorescence chemistries, we performed parentage analysis of an artificial Japanese eel population. CERVUS software was used to calculate the logarithm of the odds (LOD) scores and the confidence of the parentage assignments. The results presented here show that 83 out of 85 paternity cases were assigned at 95% confidence to a candidate father and mother with LOD scores ranging from 4.79 to 28.2. This study provided a microsatellite marker-based assay for parentage analysis of Japanese eels, which will be useful for selective breeding and genetic diversity studies.
김재헌,노은운 한국임학회 1984 한국산림과학회지 Vol.65 No.1
잣나무의 雄花를 5月 18日~25日에 採取하여 四分子期에서 未成熟花粉에 이르는 段階의 葯을 培養한 結果 callus를 誘導하였다. G.D 基本培地에 BAP 0.1ppm 및 NAA 0.1~1ppm을 組合으로 處理한 培地에서 callus發生이 좋았으며, ½濃度의 M.S培地(Sucrose 2%, Agar 0.8%)에 BAP 0.1ppm과 NAA 0.5ppm을 加한 경우에도 좋았다. callus의 chromosome數는 12인 haploid도 있었으나 대부분 24로서 diploid였다. 生長이 旺盛한 callus는 體細胞에서 起因된 것으로 생각되어 앞으로 chromosome의 變異 等을 더욱 調査하여 ploidy의 특성을 究明할 수 있으리라고 생각된다.