인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

녹색건축 전문가 자격제도 개발 연구

이상민,이승민,Lee, Sang Min,Lee, Seung Min 한국교육녹색환경연구원 2014 교육·녹색환경연구 Vol.13 No.1

In an effort to resolve environmental pollution problems, developed countries enforce green building certification systems. The same is true in Korea. However compared professional accreditation system to certification systems did not systematically in Korea. Therefore, in accordance with development of green building related systems and technology the requirement for foster green building professionals is urgent. Accordingly this study is purposed to analyze and propose that green building professional accreditation system development and operation of the direction. Through data analysis domestic and international green building professional accreditation system for draw a development plan. And then conduct an expert Delphi survey reflected in the survey results to development plan.

다슬기 배합사료 개발 현황

이상민,Lee, Sang-Min 한국양식학회 2004 韓國養殖 Vol.16 No.1

머신러닝 기법을 활용한 낙동강 중류 지역의 Chl-a 예측 알고리즘 비교 연구(수질인자 및 수량 중심으로)

이상민,박경덕,김일규,Lee, Sang-Min,Park, Kyeong-Deok,Kim, Il-Kyu 대한상하수도학회 2020 상하수도학회지 Vol.34 No.4

In this study, we performed algorithms to predict algae of Chlorophyll-a (Chl-a). Water quality and quantity data of the middle Nakdong River area were used. At first, the correlation analysis between Chl-a and water quality and quantity data was studied. We extracted ten factors of high importance for water quality and quantity data about the two weirs. Algorithms predicted how ten factors affected Chl-a occurrence. We performed algorithms about decision tree, random forest, elastic net, gradient boosting with Python. The root mean square error (RMSE) value was used to evaluate excellent algorithms. The gradient boosting showed 10.55 of RMSE value for the Gangjeonggoryeong (GG) site and 11.43 of RMSE value for the Dalsung (DS) site. The gradient boosting algorithm showed excellent results for GG and DS sites. Prediction value for the four algorithms was also evaluated through the Receiver operating characteristic (ROC) curve and Area under curve (AUC). As a result of the evaluation, the AUC value was 0.877 at GG site and the AUC value was 0.951 at DS site. So the algorithm's ability to interpret seemed to be excellent.

몰수평판에 의한 폰툰형 부유체에 작용하는 파랑강제력의 감소현상에 관한 연구

이상민,이원우,Lee, Sang-Min,Lee, Won-Woo 해양환경안전학회 2007 해양환경안전학회지 Vol.13 No.3

Pontoon type very large floating structure has been considered and actively studied as one of the most important ocean space utilization. The hydroelastic displacement of the pontoon type floating structure in waves is the largest at its weather side. The purpose of this study is to investigate the characteristics and effects of the submerged horizontal plate which is developed to reduce the wave exciting forces acting on the pontoon type floating structure using numerical analysis. The numerical method based on the finite difference method has been adopted and compared with the experimental data to confirm the reliability of it. We have performed the numerical computation of wave exciting forces acting on the pontoon type floating structure with/without submerged plate and discuss the results of simulation. 폰툰형 초대형 부체구조물은 해양공간의 활용에 있어서 가장 많이 고려되고 또한 적극적으로 연구되어지는 분야이다. 폰툰형 부체구조물의 해상에서의 유탄성 변형은 입사하는 파와 접하는 풍상측에서 가장 큰 값을 나타내고 있다. 본 연구에서는 폰툰형 부유체에 작용하는 파랑강제력을 감소시키고자 개발된 부유식 수평 몰수평판의 효과를 수치해석적인 방법에 의하여 그 특성을 파악하고자 한다. 유한차분법을 토대로 한 수치계산법을 적용하고, 적용된 수치계산법을 실험값과 비교함으로서 프로그램의 신뢰성을 확인한다. 검증된 수치계산법을 이용하여 본 연구에서 실시하고자 하는 폰툰형 부유체에 작용하는 파랑강제력의 계산을 수행하고 그 결과에 대하여 논의한다.

교통조사의 개선방향에 관한 제언

이상민,추상호,Lee, Sang-Min,Chu, Sang-Ho 대한교통학회 2005 교통 기술과 정책 Vol.2 No.4

학교시설 수선주기 설정에 관한 연구

이상민,Lee, Sang Min 한국교육녹색환경연구원 2018 교육·녹색환경연구 Vol.17 No.2

본 연구는 안전성, 노후도, 경제성 등 시설 여건을 종합적으로 고려하여 개선이 필요한 중장기적인 사업물량 및 사업예산 규모를 추정할 수 있도록 하기 위해 국가수준의 시설개선 기준(수선주기)을 설정하여 체계적인 시설개선계획 수립을 통한 적정수준의 교육환경 확보에 기여할 수 있도록 하는 것을 주목적으로 한다. 적정 수선주기는 기본적으로 최근 2년간 교육청에서 실제 집행한 교육환경개선사업의 실적자료를 분석하여 직전 보수년도와 사업시행연도를 근거로 수선주기 기초자료를 산출하였다. 이를 근거로 17개 시도교육청 실무자를 대상으로 델파이 조사를 실시하여 시도교육청의 의견을 수렴하였으며 최종 적정 수선주기는 관련규정과 실적자료, 델파이조사 결과를 종합적으로 검토하여 설정하였다. In Korea, it is difficult to secure student health and safe educational environment due to the rapid deterioration of many school facilities built in the 70s and 80s in the past. As a result, through the improvement of school facilities, social needs for safe schools from various disasters, accidents, Is increasing. The purpose of this study was to establish a standard for the establishment of the national and school facilities for the mid - to long - term plan for the improvement of the old school facilities, and to propose a framework for mid - to long - term and rational planning that can be used by the national and local government offices. The purpose of this study is to establish a mid - and long - term plan for the improvement of old school facilities. The appropriate repair cycle for the improvement of the school facilities set up in this study can be utilized as a standard for the mid - and long - term planning for improvement of the school facilities in the Ministry of Education and the School of Education. To this end, a standard model, It is possible to roughly estimate the overall improvement in the mid- to long-term facilities at the national and regional levels.

정적 O/D자료와 실시간 동적 교통정보의 생성과 관리

이상민,신희철,유재광,Lee, Sang-Min,Sin, Hui-Cheol,Yu, Jae-Gwang 대한교통학회 2005 교통 기술과 정책 Vol.2 No.1

동의보감(東醫寶鑑) 중(中) 목통(木通)이 주약(主藥)으로 배오(配伍)된 처방(處方)의 활용(活用)에 대한 고찰(考察)

이상민,권택현,옥인수,윤용갑,Lee, Sang-Min,Kwon, Taek-Hyun,Ok, In-Soo,Yun, Young-Gab 대한한의학방제학회 2005 大韓韓醫學方劑學會誌 Vol.13 No.1

This report describes the remedial fields, symptoms, pathology, dosage, prescriptional constitution of 46 studies related to the use of Lignum akebiae main blended prescriptions from Dongeubogam. The following conclusions were reached through investigations on the prescriptions that use Lignum akebiae as a key ingredient. Lignum akebiae blended prescriptions are utilized 17.4% of prescriptions appear in the chapter of urine. Prescriptions that utilize Lignum akebiae as the main ingredient are used commonly in the treatment of the symptoms of a urine and a cystic disease related to the urinary organs, or the diseases of eye, ear, nose, throat and the symptoms of a tumor, abscess. Lignum akebiae is utilized for an etiological and pathologenic fators related to heat, dampness, phlegm and summer heat such as excessive fever of heart and bladder, fever of small intestine, lower energizer dampness and heat, fever due to blood deficiency, phlegm fire, affection by summer heat and dampness etc. The clinical dosage of Lignum akebiae is 1 don(about 3.75gram), according to symptoms, it has ranged at a wide variety of amounts from 3 pun(about 1.13gram) to 1.5 don(about 11.25gram). Lignum akebiae is widely applied with base prescriptions such as Dozuksan, Moktongchajuntang, Manjunmoktongtang. etc.

학교시설 무상공급 재정성과 및 제도개선 방안 연구

이상민,Lee, Sang-Min 한국교육녹색환경연구원 2015 교육·녹색환경연구 Vol.14 No.3

본 연구는 학교시설 무상공급제도에 따른 교육재정성과를 분석하기 위해 무상공급 현황 및 재정분석, 문제점 분석을 실시하였다. 이를 위해 수도권 학교시설 무상공급 관련 사업 현황을 조사하였으며, 제도 시행성과 및 문제점에 대한 설문조사를 실시하였다. 학교시설 무상공급은 교육재정에 대한 성과에도 불구하고 법제도 미비에 따른 협약 및 공사품질확보, 적정시기 개교 등에 있어서 많은 문제점을 드러내고 있으며 이를 개선하기 위한 법제도의 보완이 필요한 것으로 분석되었다. 2009 As the School District is amended as a metropolitan area public development operators obliged to free supply of school facilities contribute to the streamlining of the provincial education funding, and although institutions shortly became implemented in earnest, the effect has been, despite a number of problems occur. In this study, analysis of these school facilities free of charge supply system on the status and problems through surveys, and suggests directions for future drafting and improving legislation.