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Profiling of the Bacteria Responsible for Pyogenic Liver Abscess by 16S rRNA Gene Pyrosequencing
송윤규,심상군,김광민,이동해,김대수,최상행,송재영,강형련,백승철,이우곤,조명제,이광호 한국미생물학회 2014 The journal of microbiology Vol.52 No.6
Pyogenic liver abscess (PLA) is a severe disease with considerablemortality and is often polymicrobial. Understandingthe pathogens that cause PLA is the basis for PLA treatment. Here, we profiled the bacterial composition in PLA fluid bypyrosequencing the 16S ribosomal RNA (rRNA) gene basedon next-generation sequencing (NGS) technology to identifyetiological agents of PLA and to provide information oftheir 16S rRNA sequences for application to DNA-basedtechniques in the hospital. Twenty patients with PLA whounderwent percutaneous catheter drainage, abscess culture,and blood culture for isolates were included. Genomic DNAsfrom abscess fluids were subjected to polymerase chain reactionand pyrosequencing of the 16S rRNA gene with a454 GS Junior System. The abscess and blood cultures werepositive in nine (45%) and four (20%) patients, respectively. Pyrosequencing of 16S rRNA gene showed that 90% of thePLA fluid samples contained single or multiple genera ofknown bacteria such as Klebsiella, Fusobacterium, Streptococcus,Bacteroides, Prevotella, Peptostreptococcus, unassignedEnterobacteriaceae, and Dialister. Klebsiella was predominantlyfound in the PLA fluid samples. All samples thatcarried unassigned bacteria had 26.8% reads on average. We demonstrated that the occurrence of PLA was associatedwith eight known bacterial genera as well as unassignedbacteria and that 16S rRNA gene sequencing was more usefulthan conventional culture methods for accurate identificationof bacterial pathogens from PLA.
Antibacterial Activity of Ulva lactuca againstMethicillin-Resistant Staphylococcus aureus(MRSA)
이재화,이동군,이상현,하종명,하배진,김성구,이재화 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.5
The in vitro antimicrobial activity of the marine green algae Ulva lactuca was examined against gram-positive bacteria, gram-negative bacteria, and a fungus. The ethyl-ether extract of algae exhibited a broad-spectrum of antibacterial activity, but not antifungal activity against Candida albicans. In particular, the U. lactuca extract showed strong activity against the bacterium methicillin-resistant Staphylococcus aureus (MRSA). This result confirms the potential use of seaweed extracts as a source of antibacterial compounds or as a health-promoting food for aquaculture.
류창환,이승진,조재구,최익준,최윤석,홍용태,정수연,김지원,이도영,이동군,이길준,이상준,이영찬,이용상,남인철,박기남,박영민,성의숙,손희영,서인효,이병주,임재열 대한이비인후과학회 2022 Clinical and Experimental Otorhinolaryngology Vol.15 No.1
Voice change is a common complaint after thyroid surgery and has a significant impact on quality of life. The Korean Society of Laryngology, Phoniatrics and Logopedics assembled a task force to establish guideline recommendations on education, care, and management related to thyroid surgery. The guideline recommendations encompass preoperative voice education, management of anticipated voice change during surgery, and comprehensive voice care after thyroid surgery, and include in-depth information and up-to-date knowledge based on validated literature. The committee constructed 14 key questions (KQs) in three categories—preoperative (KQ 1–2), intraoperative (KQ 3–8), and postoperative (KQ 9–14) management—and developed 18 evidence-based recommendations. The Delphi survey reached an agreement on each recommendation. A detailed evidence profile is presented for each recommendation. The level of evidence for each recommendation was classified as high-quality, moderate-quality, or low-quality. The strength of each recommendation was designated as strong or weak considering the level of evidence supporting the recommendation. The guidelines are primarily targeted toward physicians who treat thyroid surgery patients and speech-language pathologists participating in patient care. These guidelines will also help primary care physicians, nurses, healthcare policymakers, and patients improve their understanding of voice changes and voice care after thyroid surgery.