LPS로 유발된 대식세포의 염증반응에 대한 청상보하탕(淸上補下湯)의 효과

이경희,김홍렬,정희재,이형구,Lee, Kyung-Hee,Kim, Hong-yeoul,Jung, Hee-Jae,Lee, Hyung-Koo 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.1

Background and Objective : Chungsangboha-tang (CSBHT) has analgesic, sedative, anti-convulsive and anti-histamine effects, so it alleviates the symptoms of asthma. For the comparison of anti-inflammatory effect(s) on CSBHT, PD098059 was used as a negative control. Materials and Methods : This study emphasized THP-1 cells, which had been well characterized as a human monocytic leukemic cell line. The cells resemble monocytes with respect to several criteria and can be differentiated into macrophage-like cells by treatment with PMA. By using the MTS assay, it was possible to prove the safety of CSBHT. Results : Results shows that the CSBHT did not affected cell survival within $10^{1}$ ng/ml to $10^{5}$ ng/ml. Especially, $10^{5}$ ng/ml CSBHT treated cells show 70% deduction of $TNF-{\alpha}$ gene expression against that of LPS treated group. Furthermore, $IL-1{\beta}$, IL-6, IL-8, IL-10 and $TNF-{\alpha}$ levels are down-regulated when treated with CSBHT with concentrations up to 100 ug/ml on monocyte-derived macrophages. Interestingly, CSBHT-treated samples showed that overall transcriptional activities were down-regulated to 20% of that of PD098059 ($TNF-{\alpha}$ inhibitor). At protein level, the expression of $TNF-{\alpha}$ showed similar results as that of transcriptional activity. Results show that the protein level decreased more in the CSBHT-treated group (487 ${\pm}$ 87 pg/ml) than in the LPS-treated group (703 ${\pm}$ 103 pg/ml). In addition, the protein level of IL-8 in the CSBHT treated-group (9.84 ${\pm}$ 3.28 ng/ml) decreased similar as the expression of the control and PD098059-treated groups. Conclusion : CSBHT affects immune response, especially allergic responses and suppression of inflammatory reaction. The results provide us an alternative way to care for clinical inflammatory diseases, not only asthma but also the other possible general inflammatory and allergic diseases.

1.5 T 자기공명영상기기에서 수소 자기공명분광법을 이용한 모델용액 내 포도당의 정량분석

이경희,이정희,조순구,김용성,김형진,서창해,Lee, Kyung-Hee,Lee, Jung-Hee,Cho, Soon-Gu,Kim, Yong-Seong,Kim, Hyung-Jin,Suh, Chang-Hae 대한영상의학회 2002 대한영상의학회지 Vol.46 No.1

목적: 1.5T 생체용 자기공명영상기기를 이용한 수소자기공명분광법으로 용액 내 물질의 정량분석에 대한 가능성을 알아보고자 하였다. 대상과 방법: 0.01%에서 50%까지의 여러 농도를 갖는 포도당+증류수 혼합액과 0.01%에서 20%까지의 여러 농도를 갖는 포도당+증류수+에탄올 혼합액의 모델용액을 만들어 생체용 자기공명영상기기와 시험관 nuclear magnetic resonance(NMR)분광기에서 각각 수소자기공명분광법을 시행하여 스펙트럼을 얻었다.스펙트럼 상에서 포도당 농도에 따른 포도당/물, (포도당+에탄올)/물, (포도당+에탄올)/에탄올 피크의 면적 비의 변화를 구하였고, 통계처리는 상관분석과 단순선형회귀분석을 시행하였고 회귀식을 산출 하였다.또한 생체용 자기공명영상기기를 이용하여 얻은 결과가 객관적인지 알아보기 위해 시험관 NMR 분광기에서 얻은 결과와의 상관관계를 분석하였다. 결과: 생체용 자기공명영상기기를 이용한 스펙트럼상 포도당/물, (포도당+에탄올)/물, (포도당+에탄올)/에탄올 피크의 면적 비는 포도당 농도변화에 대하에 일정하게 증가하는 통계적으로 의미 있는 변화를 보였고 그 결과에 따라 회귀식을 구할 수 있었다. 생체용 자기공명영상기기와 시험관 NMR 분광기를 이용하여 얻은 모든 결과에 대한 상관분석에서 통계적으로 의미 있는 상관관계를 보여 생체용 자기공명영상기기에서 얻은 수소자기공명분광 소견을 객관화 할 수 있었다. 따라서 생체용 자기공명영상기기를 이용하여 얻은 스펙트럼에서 용액 내 물질의 피크면적비를 구함으로써 농도를 정량할 수 있는 결과를 얻을 수 있었다. 결론: 생체용 자기공명영상기기를 이용하여 얻은 수소자기공명분광 스펙트럼에서도 시험관 NMR분광기로 얻은 스펙트럼과 마찬가지로 단순 포도당 용액, 포도당에 에탄올을 혼합한 용액에서 포도당/물, (포도당+에탄올)/물, (포도당+에탄올)/에탄올 피크의 면적 비를 측정함으로써 당의 정량분석이 가능하였다. 본 연구의 결과는 생체수소자기공명분광법이 환자의 체액에 대한 정량적 분석에 이용될 수 있다는 가능성을 시사한다고 생각한다. Purpose: To evaluate the feasibility of proton magnetic resonance spectroscopy ($^1H-MRS$) using a 1.5T magnetic resonance (MR) imager for quantification of the contents of model solutions. Materials and Methods: We prepared model solutions of dextrose+water and dextrose+water+ethanol at dextrose concentrations of 0.01% to 50% and 0.01% to 20%, respectively. Using these solutions and a 1.5T MR imager together with a high-resolution nuclear magnetic resonance (NMR) spectroscope, we calculated the ratios of dextrose to water peak, (dextrose+ethanol) to water peak, and (dextrose+ethanol) to ethanol peak, as seen on MR and NMR spectra, analysing the relationships between dextrose concentration and the ratios of peaks, and between the ratios of the peaks seen on MR spectra and those seen on NMR spectra. Results: Changes in the ratios between dextrose concentration and dextrose to water peak, (dextrose+ethanol) to water peak and (dextrose+ethanol) to ethanol peak, as seen on MR spectra, were statistically significant, and there was good linear regression. There was also close correlation between the ratios of the observed on MR and NMR spectra. The results depict the quantification of dextrose concentration according to the ratios of spectral peaks obtained by proton MRS at 1.5T. Conclusion: Using proton MRS at 1.5T, and on the basis of the ratios of spectcal peaks, it was possible to quantify the concentration of dextrose in model solutions of dextrose+water and dextrose+water+ethanol. The results of this study suggest that for quantifying the contents of biofluids, the use of low-tesla $^1H-MRS$ is feasible.

학습환경으로서 미래교육시설구성에 대한 교사 인식 연구

이경희,홍경선,유화영,Lee, Gyeoung-Hee,Hong, Kyung-Sun,Yu, Hwa-Young 한국교육시설학회 2012 敎育施設 Vol.19 No.4

The purpose of this study is to gauge the level of awareness of elementary, middle school, and highschool teachers regarding teaching-learning activities, learning space, and support for technology-based instruction in current and future school education, thereby providing essential data for seeking future improvements in the learning environment. Towards this end, a survey questionnaire (sectionalized into teaching-learning activities, learning space, and support for technology-based instruction) was developed. Subsequently the survey was administered to a random sampling of elementary, middle school, and highschool teachers in the Kyonggi, Kangwon, and Chonra Provinces. An in-depth analysis was conducted on the 797 questionnaire responses thus attained, focusing on the teachers' level of awareness regarding teaching-learning activities, learning space, and support for technology-based instruction, the correlation of the findings of the three areas in question, and the differences of the findings according to the circumstances of those surveyed. Based on the results, the authors of this study propose the following: First, that school administrators take into account the level of awareness of frontline teachers regarding teaching-learning activities, learning space, support for technology-based instruction. Second, efforts should be made to establish a safe and secure learning environment conducive to the synergic interplay of the three areas in question, by realizing true ICT in the classroom. Third, teachers in the classroom must endeavor to achieve a meaningful synthesis between educational programs and teaching-learning activities, as well as to serve as harbingers of improvements in the way we conduct the business of education.

논문편 : 식품표시에 대한 소비자 행동 연구

이경희,여정성 ( Kyung Hee Lee,Jung Sung Yeo ) 대한가정학회 1996 Human Ecology Research(HER) Vol.34 No.5

논문편 : 부모의 언어통제유형과 아동의 사회적 능력과의 관계

이경희,정옥분 ( Kyung Hee Lee,Ock Boon Chung ) 대한가정학회 1994 Human Ecology Research(HER) Vol.32 No.5

산지별(産地別) 봉독액약침자극(峰毒液藥鍼刺戟)이 면역기능저하(免疫機能低下)에 미치는 영향(影響)

이경희,김창환,강성길,고형균,Lee, Kyung-Hee,Kim, Chang-Hwan,Kang, Sung-Keel,Koh, Hyung-Kyun 대한침구의학회 2000 대한침구의학회지 Vol.17 No.4

Objectives : This study was purposed to investigate the effect of honey bee venom aqua-acupuncture on decreased immune response by its production from Korea, North America and China. Methods : ICR-mouse was exposed with four minute sublethal 60Co- $\gamma$ -ray irradiation onto the body and got a series of each country's 0.1 ml honey bee venom aqua-acupuncture to Choksamni(ST36) every other day for three times. Thereafter the numbers of WBC, delayed type hypersensitivity, hemagglutinin titers, hemolysin titers, quantitation of T-cell and B-cell, lymphocyte transformation, natural killer cell activity, interleukin-2 productivity and interferon productivity were measured. Results : The numbers of WBC, delayed type hypersensitivity, hemagglutinin titers, hemolysin titers, quantitation of T-cell and B-cell and lymphocyte transformation were increased respectively with statistical significance in Korean, North American and Chinese honey bee venom aqua-acupuncture groups as compared with the control group. The natural killer cell activity was increased with statistical significance in Korean honey bee venom aqua-acupuncture group, and Interleukin-2 productivity and interferon productivity were not shown any statistical significance in all experimental groups as compared with the control group. Conclusions : According to the results, honey bee venom aqua-acupuncture has significant effect on decreased immune response, and Korean bee venom can be substituted for North American and Chinese one.

윈도우 커버링용 PET 난연 직물의 마모 및 역학특성

이경희,전연희,안승국,Lee, Kyung Hee,Jeon, Youn Hee,An, Seung Kook 한국섬유공학회 2013 한국섬유공학회지 Vol.50 No.2

This study examined the abrasion and mechanical properties of poly(ethylene terephthalate) (PET) flameretardant fabrics used for window coverings. The tensile linearity, tensile resilience, bending rigidity, bending hysteresis, shearing stiffness, shearing hysteresis, compression energy, and surface roughness all increased with increasing fabric density and cover factor ratio. Five samples were subjected to more than 16,000 cycles for the abrasion resistance test and to grade 4 for the snagging test. The drape test showed that the flexibility of the samples was affected by fabric density and cover factor ratio.

장애 아동 아버지의 스트레스와 적응에 관한 구조 모형

이경희,Lee, Kyung-Hee 대한간호협회 1992 대한간호 Vol.31 No.5

This study was designed to develop and lest a structural model for paternal adaplation and family stress in the families with the handicapped children. A hypothetical model was constructed on the basis of family stress theory developed by McCubbin etc. The model included 6 theoretical concepts and 11 paths. For the purpose of the model testing, empirical data were collected from May to August, 1992. The subject of the study constituted 190 mothers whose children admitted in the five special schools and one privale institutes in Seoul and Choong Nam. In data analysis, SPSS PC+ and PC-LISREL 7.13 computer program were utilized for descriptive and covariance structure analysis. The results of lhe study were as follows. (1) Hypothetical model showed a good fit to the empirical data [Chi-square = 21.19 (df = 7, P = o. 003), Goodness of Fit Index=O.986, Adjusted Goodness of Fit Indes=0.946, Root Mean Square lesidual=0.048), Non Normed Fit Index = 0.80, Normed Fit Index=0.91]. (2) The results of Hypothesis testing indicated: 1) Social support(${\gamma}_11=.238$, T=2.352), Family Functioning(${\gamma}_12=.729$, T=5.957) had direct effects on situational definition. 2) life event stress(${\gamma}_23=.284$, T=5.220) had direct effects on the effect of the handicapped chid on the family. 3) Family functioning(${\gamma}_32=-.239$, T= -3.370) had direct effects on paternal adapation. From the above results, the significance of this study can be delineated as follows : The construction and testing of the comprehensive model seem to be the first trial in Korea. The model was supported by empirical dala. Thus it was suggested that model could be adequately applied to framily nusing care with the handicapped.

물리치료학에서의 문제중심학습(Problem Based Learning)

이경희,김철용,김성학,Lee, Kyung-Hee,Kim, Chul-Yong,Kim, Seong-Hak 대한물리치료과학회 2002 대한물리치료과학회지 Vol.9 No.4

Problem based learning(PBL) is one of the learning strategies from the constructivism. It is a learning centered students. The tutors are facillitators as activators, helpers and cooperators not organizer in the classrooms. PBL makes that students learn creativity, independence, reasoning skits, communication and collaboration for problem solving. As the PBL process, students get the problems that are in real situation, discussed with others for brain storming, self directed study and revisited to the situation. They think critically and apply to the real situation. When students are to be physical therapists, they are easy to adopt their job and efficient to manage well. But inspite of a lot of advantages to them, there are much conflict to use as the learning strategies. Students perceived one of best learning method that they have experienced, but there are stress, burden, anxiety, timeless to prepare, lack of information and so on. PBL is effective to learning health oriented subjects, problem solving, even a lot preparation and processing for learning. It is reduced the differences between theories in colleges and practices in the fields. In processing of PBL, students get more many skills than the conventional learning. As trying many times to the classrooms, we can fixed to PBL with mistakes and conflict for better the development of the teaching and learning.

실측을 통한 신축공동주택의 실내공기질에 관한 연구

이경희,배종수,조성우,박민용,박창섭,최정민,Lee Kyung-Hee,Bae Jong-Soo,Cho Sung-Woo,Park Min-Yong,Park Chang-Sub,Choi Jeong-Min 한국주거학회 2006 한국주거학회 논문집 Vol.17 No.4

The harmfulness of HCHO and VOCs from construction material and furniture has been increased gradually. It must be need to prevent pollution materials' accumulation indoor effectively and to remove very small amount of harmful pollution materials in various plans, because these kinds of pollution materials greatly affect human body, Therefore, this study is focused to find out the improvements of Indoor Air Quality in execution of natural ventilation and bakeout to reduce indoor chemical pollution materials or not. After effects of indoor air quality by natural ventilation and bake-out being examined, it is follows the conclusion. As for the density change of TVOC and Toluene according to time lapse, in case the middle and high-story areas have bake-out, the density increased once, but it showed the gradual decrease after bake-out was stopped, and it was shown that it exceeded the standards recommended for newly built apartment. The bake-out is effective to discharge the HCHO and TVOC from the construction material and the furniture, and the natural ventilation is effective remove the indoor pollution materials.