내분비장애물질노출이 유방암에 미치는 영향 -비스페놀 A를 중심으로

양미희 대한암예방학회 2006 Journal of cancer prevention Vol.11 No.4

Incidence ratio of breast cancer to total cancer has been increased in late 2 decades in Korea and breast cancer ranks second as a cause of death in women in recent Korea. In addition, exposure to endocrine disruptors has been emphasized as a new etiology of breast cancer. In this study, the effects of bisphenol A exposure on breast cancer in Korean women (100 breast cancer patients; 100 hospital controls) were investigated. Conjugated form of blood bisphenol A was designated as an exposure biomarker and analyzed with HPLC/FD. As results, blood bisphenol A could be detected in 94% of the subjects and the range of blood bisphenol A was 0.69~33.7μg/L (median, 5.3μg/L). The geomeric mean of bisphenol A was somewhat higher in the cases than that in the controls, however, was not statistically significant due to multivariate analysis including age, alcohol dringking, breast feeding, and age of menarche. Thus, bisphenol A exposure may not work as a breast cancer risk factor. (Cancer Prev Res 11, 273-277, 2006)

종자검정 발전의 역사

양미희,소은희 한국종자연구회 2010 종자과학과 산업 Vol.7 No.2

한국산 배(梨)를 중심한 과수의 기능성 연구

양미희 한국원예학회 2006 한국원예학회 학술발표요지 Vol.2006 No.5

In my previous study, consumption of Korean pears had inverse association with level of urinary 1-hydroxypyrene (1-OHP), which is an exposure biomarker and metabolite of polycyclic aromatic hydrocarbons (PAHs). Bioproduction of reactive intermediate of PAHs or induction of oxidative stress have been suspected as the mechanisms of PAHs-related diseases such as lung cancer, asthma, etc. Thus, to clarify potential of functional food in the Korean pears against PAHs-exposure, I studied effects of the Ko rean pears on 1. PAHstoxicokinetics, 2. PAHs- induced oxidative stress in intervention trials (N=30; male, N=10; female, N=20), and 3. PAHs- related chronic obstructive pulmonary disease (COPD: N= 30). The intake of Bulgogi, Korean barbeque, was used as exposure to PAHs. After 3 Ieks trials for the same subjects (1 week for Bulgogi only, Bulgogi with the pears, and the pears only, respectively), I found rapid excretion of urinary 1-OHP with the pears during 24 hrs compared to that without pears (0.05<p<0.1). In addition, I analyzed urinary levels of malondialdehyde (MDA), a biomarker for oxidative stress, following time cmyse. As results, I found that the Bulgogi induced MDA and the pears reduced the Bulgogi- induced MDA. I also analyzed another biomarker for oxidative stress due to PAHs, i.e. change in dichlorodihydro fluorescein (DCF). As a result, the DCF levels Ire not affected by intake of Bulgogi or the pears. HoIver, intake of the pear (half of a pear per day for 1 month) did not show significant improvement of ‘the St George’s respiratory questionnaire score’ or ‘forced expiratory volume for 1 second (FEV). In conclusion, my results suggest that the Korean pears have chemoprevention effects on PAHs- induced carcinogenic mechanisms due to rapid excretion of PAHs- intermediates and reduction of PAHs- induced oxidative stress. However, the pear-chemopreventive effects against PAHs-related diseases should be further clarified in enlarged population studies.

한국인 다기관 암 코호트 시료의 DNA 생활성도 평가

양미희,유지현,김청식,신애선,강대희,장성훈,박수경,신해림,유근영,Yang, Mi-Hi,Yoo, Ji-Hyun,Kim, Cheong-Sik,Shin, Ae-Sun,Kang, Dae-Hee,Chang, Soung-Hoon,Park, Sue-Kyung,Shin, Hai-Rim,Yoo, Keun-Young 대한예방의학회 2003 예방의학회지 Vol.36 No.4

Objectives : Peripheral blood-buffy coat fractions (N=14,956) have been stored at $-70^{\circ}C$ in the headquarter of the Korean Multicenter Cancer Cohort (KMCC), since 1993. To study the future molecular etiology of cancers using specimens of the cohort, properly stored specimens are necessary, Therefore, the DNA-viability of the bully coat samples was investigated. Methods : Buffy coat fraction samples were randomly selected from various collection areas and years (N=100). The DNA viability was evaluate from the UV-absorbent ratios at 260/280nm and the PCH for $\beta$-globin was performed with genomic DNA isolated from the buffy coat. Results : PCR products were obtained from 85 and 98% of the C and H area-samples, respectively, using 50 or $100{\mu}l$ of the buffy coat. There were significant differences in the yields of the PCR-amplifications from the C and H areas (p<0.05), which was due to differences in the homogenization of the buffy coat fractions available as aliquots. The PCR-products were obtained from all of the samples (N=7) stored at the C area-local confer, but the other aliquots stored at the headquarter were not PCR-amplified, Therefore, the PCR products in almost all the samples, even including the DNA-degraded samples, were obtained. In addition, an improvement in the DNA isolation, i,e. approx. 1.6 fold, was found after using extra RBC lysis buffer. Conclusions : PCR products for $\beta$-globin were obtained from nearly all of the samples. The regional differences in the PCR amplifications were thought to have originated from the different sample-preparation and homogenization performance. Therefore, the long term-stored buffy coat species at the KMCC can be used for future molecular studies.

[서평 (3)] 성숙한 신앙으로 삶을 살아 내기 위한 한 이성적 접근

양미희 대한기독교서회 2015 基督敎 思想 Vol.676 No.-

한국산 배(梨)를 중심한 과수의 기능성 연구

양미희 한국원예학회HST 2006 원예과학기술지 Vol.24 No.별호

In my previous study, consumption of Korean pears had inverse association with level of urinary 1-hydroxypyrene (1-OHP), which is an exposure biomarker and metabolite of polycyclic aromatic hydrocarbons (PAHs). Bioproduction of reactive intermediate of PAHs or induction of oxidative stress have been suspected as the mechanisms of PAHs-related diseases such as lung cancer, asthma, etc. Thus, to clarify potential of functional food in the Korean pears against PAHs-exposure, I studied effects of the Ko rean pears on 1. PAHstoxicokinetics, 2. PAHs- induced oxidative stress in intervention trials (N=30; male, N=10; female, N=20), and 3. PAHs- related chronic obstructive pulmonary disease (COPD: N= 30). The intake of Bulgogi, Korean barbeque, was used as exposure to PAHs. After 3 Ieks trials for the same subjects (1 week for Bulgogi only, Bulgogi with the pears, and the pears only, respectively), I found rapid excretion of urinary 1-OHP with the pears during 24 hrs compared to that without pears (0.05<p<0.1). In addition, I analyzed urinary levels of malondialdehyde (MDA), a biomarker for oxidative stress, following time cmyse. As results, I found that the Bulgogi induced MDA and the pears reduced the Bulgogi- induced MDA. I also analyzed another biomarker for oxidative stress due to PAHs, i.e. change in dichlorodihydro fluorescein (DCF). As a result, the DCF levels Ire not affected by intake of Bulgogi or the pears. HoIver, intake of the pear (half of a pear per day for 1 month) did not show significant improvement of ‘the St George’s respiratory questionnaire score’ or ‘forced expiratory volume for 1 second (FEV). In conclusion, my results suggest that the Korean pears have chemoprevention effects on PAHs- induced carcinogenic mechanisms due to rapid excretion of PAHs- intermediates and reduction of PAHs- induced oxidative stress. However, the pear-chemopreventive effects against PAHs-related diseases should be further clarified in enlarged population studies.

CYP1A1 유전자발현과 소아천식의 상관

양미희 환경독성보건학회 2005 환경독성보건학회지 Vol.20 No.3

대학생의 결혼관에 대한 연구

양미희,권혜성 명지대학교 1986 家政學硏究 Vol.2 No.-

내부표준물질, 9-hydroxyphenanthrene을 이용한 뇨 중 1-hydroxypyrene의 새로운 정량법

양미희,이호선,신민경,김용학 숙명여자대학교 약학연구소 2005 약학논문집-숙명여자대학교 Vol.22 No.-

Urinary 1-hydroxypyrene (1-OHP) has been used as exposure biomarkers for polycyclic aromatic hydrocarbons and analyzed with HPLC/FD after preparation by column extraction or simple centrifuge. However, the above methods have several drawbacks such as high noise or large volume of urine. In this study, we developed a new analysis method with liquid-liquid extraction and purification of commercial enzymes. We analyzed uirnary 1-OHP in 27 spot urines ofa subject in 3 weeks with the new method. As result, we could analyze all 27 samples clearly. The resolution of the new method was better than the previous centrifuge method. Therefore, the new method is a recommendable analysis method for urinary 1-OHP. Considering both of easiness and accuracy of the experiments, the new method should be further compared with the previous methods.

소아 중환자실에서의 진정제 사용 현황과 금단현상과의 상관관계 분석

양미희,배혜정,박지언,정애희,정선회,한현주,조윤희,조윤숙,최유현,박준동 한국병원약사회 2020 병원약사회지 Vol.37 No.1

Background : Although sedatives are commonly used in intensive care units, they may cause side effects such as delayed weaning of mechanical ventilation and withdrawal syndrome. There is no adequate assessment tool for evaluating the level of sedation in children and there is a lack of studies related to the use of sedatives in children. The purpose of this study was to investigate the use of sedatives in the pediatric intensive care unit (PICU) and to analyze the frequency and related factors of withdrawal syndrome. Methods : From January 1 through June 30, 2016, we retrospectively reviewed the electronic medical records of pediatric patients admitted to the PICU at the Seoul National University Children’s Hospital. The use of sedatives, tapering status, and frequency of withdrawal syndrome was investigated. Results : Ninety-three patients were treated with sedatives in the PICU at the Seoul National Children’s Hospital. Sixty-six were surgical patients, and 27 were medical patients. A combination of midazolam and fentanyl were the most commonly used sedative and analgesic (n=78, 83.9%). The median period of sedation was 60.1 hours (interquartile range (IQR) 75.8) in the surgical patients and 117.5 hours (IQR 121.2) in the medical patients. Compared to the surgical patients, long-term sedation was applied in medical patients. Tapering off of sedatives was performed in 40 patients (43.0%). The median total duration of tapering of sedatives was 16.0 hours (IQR 26.5) in the surgical patients and 30.2 hours (IQR 62.0) in the medical patients. The withdrawal syndrome occurred in 33 patients (37.1%) among 89 patients using midazolam as the main sedative. The duration of midazolam infusion was significantly associated with the frequency of withdrawal syndrome (Spearman’s rho 0.345, p=0.049). Conclusion : The withdrawal syndrome of sedatives was associated with the duration of sedation. Thus, more attention should be focused on tapering and monitoring the occurrence of withdrawal syndrome in patients requiring prolonged sedation.