CYP1A1 유전자발현과 소아천식의 상관

양미희 환경독성보건학회 2005 환경독성보건학회지 Vol.20 No.3

Individual Variations in Toxicity of Tobacco Smoking

양미희 대한암예방학회 2005 Journal of cancer prevention Vol.10 No.3

Tobacco smoking is a well-known cause of cancers and has been emphasized not only to clarify the mechanism of carcinogenesis but also to prevent the smoking-related cancers. In addition, the Korean female passive smokers showed early onset of lung cancer and high incidence of adenocarcinoma compared to males. Therefore, several factors are suspected as etiology of female passive smokers, e.g. ETS (environmental tobacco smoking), and high sensitivity to carcinogens via genetic and hormonal factors. Traditional epidemiological data showed controversial results of sex or gender differences in lung cancer susceptibility. Therefore, many confounders were expected in those studies. Among them, one is due to self reported smoking, therefore, the effects of ETS were not thoughtfully considered. The consequence of this is that there may be an overestimation of baseline lung cancer incidence or underestimation of the ORs in female smoke. To obtain undistorted mechanisms of etiology in side stream females, proper exposure monitoring of ETS and considering of susceptibility markers are recommended. For the exposure monitoring, urinary cotinine or naphthols are recommendable, while individual variation in their metabolisms, such as genetic polymorphisms in CYP2E1 and 2A6 and GSTT1 and GSTM1 among Asians should be understood. For susceptibility factors of lung cancer in passive smoker females, genetic polymorphisms of the CYP1A1 exon 7 Ile462Val and the GSTP1 exon 5 Ile105 Val are recommended. Sensitive to tobacco carcinogenic PAHs or CYP1A1 and ER cross talk are possible mechanism of the susceptibility biomarkers in the passive smoker females. (Cancer Prevention Res 10, 135-148, 2005)

한국산 배(梨)의 항돌연변이 및 항암효과

양미희,박장환,김대중,정헌상 대한암예방학회 2005 Journal of cancer prevention Vol.10 No.2

We already reported that consumption of Korean pears had inverse association with level of urinary 1-hydroxypyrene (1-OHP), which is an exposure biomarker and metabolite of polycyclic aromatic hydrocarbons (PAHs). As PAHs include various carcinogens such as benzo(a)pyrene, our report showed the chemopreventive potential of Korean pears. Recently, we studied effects of Korean-pears on PAH-induced mutagenicity and carcinogenicity, i.e. antimutagenic effects with MN (micronuclei) test and Comet assay, and anticarcinogenic effects with animals. Finally, we performed an intervention study with the pears in volunteers using toxicokinetic methods. As results, we found that Korean pears have antimutagenic, antioxidative, and anticarcinogenic effects against PAHs exposure and rapid excretion of parental PAHs. Therefore, the intake of pears after PAHs exposure such as BBQ is thought as an ideal lifestyle to prevent PAH-induced cancers. (Cancer Prev Res 10, 124-127, 2005)

DNA 마이크로어레이를 이용한 내분비장애 물질 노출지표 개발

양미희 환경독성보건학회 2005 환경독성보건학회지 Vol.20 No.4

장기간 노출 시 발암 등 인체 유해성을 갖는 환경유래 내분비장애물질(endocrine disrupting chemicals,EDCs)에 대한 선택적이고 민감한 노출지표를 개발하기 위하여 본 연구에서는 DNA microarray를 이용하였다. 피험자는 아직 특별한 질환을 갖지 않는 18세 이 상 연 령, 성을 맞춘 EDCs 고농도 노출군(N=16)과저농도군(N=16)으로 구성되었다. 노출정도 구분은 10년 이상 거주지가 K산업폐기물 소각장과 2.5km 반경 내, 외 인지에 따라 고노출군, 저노출군으로 구분하였다. 피험자의 말초혈에서 total RNA를 분 리, 각 군당 3인씩 pool로 cDNA를 합성하여 oligonucleotide DNA 칩에 적용하였다. 유전자발현의 차이를GenePixPro 4.0 software를 이용하여 분석하였다. 총 3장의 칩을 이용하여 공통적으로 저노출군보다 고노출군에서 2배 이상 발현의 증가를 보인 유전자는 plasminogen activator(PLAT) 등 12종이 관찰되었고, 1/2이하로 발현의 감소를 보인 유전자는 kallikrein 3(KLK3) 등 29종이었다. 이 들 유전자는 PLAT 등 면역계반응에 관여하는 유전자 및 암화, 발생(development), 대사 등에관여하는 유전자들이었다. 그러므로 KLK3 등 본 연구에서 발굴한 유전자는 향후 확대된 인구에서 본 연구 결과의 확인을 통하여 EDCs 특이적 노출지표로써, 나아가 암 등 EDCs관련 질병의 기전 및 병인학을구명하는데 이용가치가 높다고 사료된다.

한국산 배(梨)를 중심한 과수의 기능성 연구

양미희 한국원예학회 2006 한국원예학회 학술발표요지 Vol.2006 No.5

In my previous study, consumption of Korean pears had inverse association with level of urinary 1-hydroxypyrene (1-OHP), which is an exposure biomarker and metabolite of polycyclic aromatic hydrocarbons (PAHs). Bioproduction of reactive intermediate of PAHs or induction of oxidative stress have been suspected as the mechanisms of PAHs-related diseases such as lung cancer, asthma, etc. Thus, to clarify potential of functional food in the Korean pears against PAHs-exposure, I studied effects of the Ko rean pears on 1. PAHstoxicokinetics, 2. PAHs- induced oxidative stress in intervention trials (N=30; male, N=10; female, N=20), and 3. PAHs- related chronic obstructive pulmonary disease (COPD: N= 30). The intake of Bulgogi, Korean barbeque, was used as exposure to PAHs. After 3 Ieks trials for the same subjects (1 week for Bulgogi only, Bulgogi with the pears, and the pears only, respectively), I found rapid excretion of urinary 1-OHP with the pears during 24 hrs compared to that without pears (0.05<p<0.1). In addition, I analyzed urinary levels of malondialdehyde (MDA), a biomarker for oxidative stress, following time cmyse. As results, I found that the Bulgogi induced MDA and the pears reduced the Bulgogi- induced MDA. I also analyzed another biomarker for oxidative stress due to PAHs, i.e. change in dichlorodihydro fluorescein (DCF). As a result, the DCF levels Ire not affected by intake of Bulgogi or the pears. HoIver, intake of the pear (half of a pear per day for 1 month) did not show significant improvement of ‘the St George’s respiratory questionnaire score’ or ‘forced expiratory volume for 1 second (FEV). In conclusion, my results suggest that the Korean pears have chemoprevention effects on PAHs- induced carcinogenic mechanisms due to rapid excretion of PAHs- intermediates and reduction of PAHs- induced oxidative stress. However, the pear-chemopreventive effects against PAHs-related diseases should be further clarified in enlarged population studies.

물 중 미세플라스틱 분석방법 표준화 연구

양미희,최인철,손보영,정아름,정지혜,이수정,서민정,박상민,박준원,윤정기,이수형 한국분석과학회 2023 학술대회논문집 Vol.2023 No.5

한국인에서 DPD 및 TS, MTHFR의 유전자다형

양미희 숙명여자대학교 약학연구소 2004 약학논문집-숙명여자대학교 Vol.21 No.-

In-order to do efficient treatment of 5-Fluorouracil (5-FU) in Koreans, we investigated genetic polymorphisms of dihydropyrimidine dehydrogenase (DPD; gene, DPYD), thymidylate synthase (75), and methyltetrahydrofolate reductase (MTHFR), which are involved in 5- FU metabolism or efficacy in a Korean population. As results, we found genetic polymorphisms in DPYD-85, -1627, and -1896 site, TS-5'ER, and -3'U'TR, and MTHFR-222, 429 site among the Koreans (normal, N=105; head and neck cancer patients, N=28). 5-FU efficacy was determined into PR(partial response, decrease of cancer size over 50%) and SD (stable disease). Each or combination of the above 7 genetic polymorphisms were not associated with 5-FU efficacy. Therefore, the studied 7 genotypes can not be considered to result in individual variation in 5-FU efficacy.

[서평 (3)] 성숙한 신앙으로 삶을 살아 내기 위한 한 이성적 접근

양미희 대한기독교서회 2015 基督敎 思想 Vol.676 No.-

내분비장애물질노출이 유방암에 미치는 영향 -비스페놀 A를 중심으로

양미희 대한암예방학회 2006 Journal of cancer prevention Vol.11 No.4

Incidence ratio of breast cancer to total cancer has been increased in late 2 decades in Korea and breast cancer ranks second as a cause of death in women in recent Korea. In addition, exposure to endocrine disruptors has been emphasized as a new etiology of breast cancer. In this study, the effects of bisphenol A exposure on breast cancer in Korean women (100 breast cancer patients; 100 hospital controls) were investigated. Conjugated form of blood bisphenol A was designated as an exposure biomarker and analyzed with HPLC/FD. As results, blood bisphenol A could be detected in 94% of the subjects and the range of blood bisphenol A was 0.69~33.7μg/L (median, 5.3μg/L). The geomeric mean of bisphenol A was somewhat higher in the cases than that in the controls, however, was not statistically significant due to multivariate analysis including age, alcohol dringking, breast feeding, and age of menarche. Thus, bisphenol A exposure may not work as a breast cancer risk factor. (Cancer Prev Res 11, 273-277, 2006)

종자검정 발전의 역사

양미희,소은희 한국종자연구회 2010 종자과학과 산업 Vol.7 No.2