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김예진(Yae Jin Kim),안혜련(Hye Ryun An),박필만(Pil Man Park),이수영(Su Young Lee),박부희(Pue Hee Park) 한국육종학회 2021 한국육종학회지 Vol.53 No.3
Cymbidium ‘Sodam’, derived from a cross between ‘Baek Un’ and ‘Lapin Rose’ in 2000, was bred by the National Institute of Horticulturaland Herbal Science, Rural Development Administration in 2015. After artificial crossing, 120 seedlings were obtained through in vitro seedgermination and seedling acclimatization in a greenhouse. On the basis of mature plant characteristics, including flower color, plant size, leafshape, flower stalk, and vigorous growth, three lines were initially selected. The first and second trials for characteristics were conductedfrom 2009 to 2014, which enabled confirmation of the stability and uniformity of the three lines. Amongst these, the final line, with thecode ‘00-1023-20’, was selected as ‘Wongyuo F1-58.’ After evaluating consumer preferences, the line was subsequently named ‘Sodam’. Thishybrid is a small- to medium-sized cultivar with more than 10 pink flowers per stalk and an average number of 3.8 stalks. The relativelycompact flower has an incurved shape with a width and length of 6.0 and 5.0 cm, respectively. Furthermore, the plants have short erectstalks and leaves (length 51.7 and 69.5 cm, respectively), showing good properties with respect to potting. The flowers have a fragrancesimilar to that of the female parent ‘Baek Un’. ‘Sodam’ commences flowering in early December. The proliferative ability of ‘Sodam’ is comparableto that of the control cultivar, and it is anticipated that this will facilitate mass proliferation for commercial use (Registration No. 6749).
국내 육성 장미 품종 꽃잎 유래 체세포배 발생 캘러스 유도
이수영,신주영,이영아,안창호,김예진,박필만,안혜련,이가연,정현환,Lee, Su Young,Shin, Ju Young,Lee, Young Ah,Ahn, Chang Ho,Kim, Yae Jin,Park, Pil Man,An, Hye Ryun,Lee, Ka Youn,Jung, Hyun Hwan 한국자원식물학회 2022 한국자원식물학회지 Vol.35 No.5
This study was conducted to induce somatic embryogenic callus (SEC) derived from petals in rose. The petal explants of 3 cultivars ('Ice Wing', 'Orange Eye' and 'Pink Beauty') with different flower colors were placed on three types media (MS, SH and WPM) supplemented with 11 mg/L 2,4-D, respectively, and then cultured in the dark for 47 days. Calluses were formed at explants of all three cultivars. Also, 'Ice Wing', which were cultured in the SH as the basal medium, showed the highest callus formation rate. However, somatic embryos were generated from only petal-derived callus of 'Ice Wing', which were induced on the WPM as the basal medium, transferred it to SH basal medium supplemented with 3 mg/L 2,4-D, and 300 mg/L L-proline, and cultured for 5 weeks. The SEC has been proliferated every four weeks at the subculture interval. In addition, as a results of making a comparison of expression of RhSERK3 and RhSERK4, which is used as signal for generation of somatic embryo from callus in rose, between the SEC and petal-derived callus from 'Ice Wing' by RT-qPCR, the former showed 10 times higher RhSERK3 expression and 700 times higher RhSERK4 expression than the latter.