배 검은별무늬병 저항성 "원교 나-흑성 2호"

신일섭,황해성,신용억,허성,김기홍,강삼석,김윤경 한국육종학회 2009 한국육종학회지 Vol.41 No.3

Venturia nashicola에 의한 검은별무늬병 저항성 품종을 육성하기 위하여 저항성 유전자원 탐색을 1997~1999년까지 국립원예특작과학원 포장검정 및 인공 접종을 실시하여 저항 성친으로 서양배 "청마려"을 선발하였다. 농촌진흥청 국립원예특작과학원 과수과에서 고품질 만생종 "만수"에 "청마려"를 1997년에 교배하여 저항성 우량 계통인 중간모본 "원교나-흑성 2호" 을 2008년에 선발하였다. 2008년 만개일은 4월 19일로 "만수" 보다 1 "Wonkyo Na-heukseong 2" was selected from a cross between "Kiyomaro", late season European cultivar with highly resistance and "Mansoo", late season Asian cultivar with long storability, large size and low susceptibility to pear scab made in 1997 at the National Institute of Horticultural & Herbal Science of Rural Development Administration in Korea. "Kiyomaro", released cross between "Taiheiyo" and "Bartlett" with scab resistance caused by Venturia nashicola in Japan, with no visual symptoms on any leaves was used as scab resistant source after field investigation and artificial inoculation test during 1997~1999. "Wonkyo Na-heukseong 2" blooms 1 day earlier than "Mansoo" and 3 days later than "Kiyomaro" in 2008. It is strong in tree vigor and upright-spreading in tree habit. It is classified as highly resistant to pear scab as "Kiyomaro" and "Bartlett", and cross-compatible with parental variety and Korean major pear varieties such as "Niitaka" and "Wonwhang". The average optimum harvest time of "Wonkyo Na-heukseong 2" was approximately 180 days after full bloom and it matured about 20 days shorter than parental varieties. The fruit is spindle in shape and yellowish greenish brown in skin color. Average fruit weight was 484 g and soluble solids content was 13.2^ºBrix. The flesh had medium to high juice and negligible grit. Its fruit was crisp like Asian pear.

윌슨의 직접 도덕교육방법

신일섭 한국도덕교육학연구회 2001 도덕교육학연구 Vol.2 No.-

연료전지의 최신기술 동향

신일섭,Sin, Il-Seop 기술표준원 2005 기술표준 Vol.37 No.-

『新靑年』雜誌(1915-1926)의 성격변화연구

신일섭 전남사학회 1991 역사학연구 Vol.5 No.-

배 자가결실성 "원교 나-자수정 2호"

신일섭,신용억,황해성,허성,김기홍,강삼석,김윤경 한국육종학회 2009 한국육종학회지 Vol.41 No.2

배는 배우체 자가 불화합성을 보이며, 이것은 단일 유전자좌의 복대립 유전자에 의하여 제어된다. '원교 나-자수정 2호'는 농촌진흥청 국립원예특작과학원에서 고품질 조생종 '원황'에 '92-18-79' (오사이십세기 자식계통, S4smS4sm)를 2001년에 교배하여 얻은 계통 중에 자가 결실성이며 품질이 우수하여 중간모본으로 선발되었다. 만개기는 '오사이십세기'보다 1일 정도 빠르고 '원황'과는 유사하다. 수세는 중, 수자는 반개장형이다. 검은별무늬병(V Pear has a gametophytic self-incompatibility (SI) system and its SI reaction is controlled by a single multi-allelic S-locus. 'Wonkyo Na-jasoojung 2' was selected from a cross between 'Wonwhang', early season major pear cultivar with high fruit quality and self-incompatible, and 92-18-79 (S4smS4sm) obtained from self cross of 'Osa-nijisseiki' (S2S4sm) (SM, stylar-part mutant), self-compatible bud mutant that originated from self-incompatible 'Nijisseiki' (S2S4) made in 2001 at the National Institute of Horticultural and Herbal Science, Rural Development Administration in Korea. '92-18-79' was selected as a self-compatible source through field investigation. It bloomed 1 day earlier than 'Osa-Nijisseiki' and similar to 'Wonwhang' in 2008. It is medium in tree vigor and spreading in tree habit. 'Wonkyo Na-jasoojung 2' is classified as highly susceptible to pear scab (Venturia nashicola) similar to 'Osa-Nijisseiki' and as resistant to black spot (Alternaria kikuchiana) similar to 'Wonwhang'. It had 65.7% fruiting rate by self pollination. The average optimum harvest time of 'Wonkyo Na-jasoojung 2' was 148 days after full bloom and it matured 2 days earlier than 'Osa-Nijisseiki' and 11 days later than 'Wonwhang'. The fruit is roundish oblate in shape and yellowish brown in skin color. Average fruit weight was 445 g and soluble solids content was 13.3 ºBrix. The flesh had abundant juice and negligible grit.

산사의 밤

신일섭 고요아침 2015 열린시학 Vol.20 No.1

수도용 기자재(볼밸브) 사용에 대한 논고

신일섭,Sin, Il-Seop 기술표준원 2003 기술표준 Vol.20 No.-

배 검은별무늬병 저항성 ‘흑성 1호’

신일섭,신용억,황해성 한국원예학회 2008 원예과학기술지 Vol.26 No.suppl2

<P>Venturia nashicola에 의해 동양배에 발생하는 검은별무늬병은 국내 배 재배에 가장 큰 피해를 주는 병이다. 이 병에 저항성 품종을 육성하기 위하여 저항성 유전 자원 탐색을 1997~1999년까지 원예연구소 포장 및 인공 접종을 실시하여 저항성친으로 서양배 ‘Bartlett’을 선발하였다. 농촌진흥청 국립원예특작과학원 과수과에서 고품질 중생종 ‘선황’에 ‘Bartlett’을 2000년에 교배하여 저항성 우량 계통인 중간모본 ‘흑성 1호’를 2007년에 선발하였다. 만개일은 4월 25일로 ‘선황’보다 1일 늦고, ‘Bartlett’보다 4일 정도 빠르며 수세는 강하고 수자는 직립형이다. 검은별무늬병에 ‘Bartlett’과 같이 강하고 ‘신고’, ‘원황’, ‘화산’ 등 국내 주요 재배 품종들과 교배 친화성이 있다. 과실은 만개 후 150일경에 성숙되어 양친에 비해 10일 정도 성숙 일수가 길며, 과형은 편원, 과피는 암황갈색이다. 평균과중은 249g, 당도는 13.2°Brix이며 과즙은 중~다이다 석세포가 적고 서양배와 같이 향기가 있으며 동양배의 아삭아삭함이 있다.</P>

배 자가 결실성 ‘자수정 1호’

신일섭,황해성,신용억,강삼석 한국원예학회 2008 원예과학기술지 Vol.26 No.suppl2

<P>장미과에 속하는 배는 배우체 자가 불화합성을 보이며, 이것은 단일 유전자좌의 복대립 유전자에 의하여 제어된다. ‘자수정 1호’는 농촌진흥청 국립특작과학원에서 고품질 중생종 ‘화산’에 ‘92-18-34’(오사이십세기 자식계통, S₄<SUP>sm</SUP>S₄<SUP>sm</SUP>)를 2000년에 교배하여 얻은 계통 중에 자가 결실성 및 과실, 생육 특성 조사를 통해 선발되었다. 만개기는 ‘오사이십세기’와 ‘화산’보다 1일 정도 늦으며 수세는 중, 수자는 반개장형이다. 검은별무늬병(Venturia nashicola)에는 ‘신고’ 정도로 약하고 검은무늬병(Alternaria kikuchiana)에 ‘오사이십세기’ 정도로 약하다. 자가 결실률은 72.7%로 안정된 자가 결실률을 보였다. 만개후 성숙기까지는 147일로 ‘오사이십세기’보다는 7일, ‘화산’보다는 10일 이상 빠르다. 과형은 원편원이고 과피는 황갈색이다. 평균 과중은 308g으로 ‘오사이십세기’보다 크며 당도는 13.9°Brix로 높고 산미가 있어 감산이 조화된 식미가 있으며 과즙은 풍부하고 석세포는 적다.</P>

Identification of Genes Induced by Venturia nashicola in Indigenous Korean Pear ‘Hwangsilri’

신일섭,배경미,남가영,강병철,천재안,조강희,김세희,최형석,김현란,황해성,이희재 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.6

Indigenous Korean pear ‘Hwangsilri’ leaves were inoculated with Venturia nashicola to build expressed sequence tags (ESTs) database as resources for transcripts induced in the inoculated leaves. After performing subtractive suppression hybridization using cDNA of the inoculated and uninoculated leaves harvested at 1, 48, and 96 hours after inoculation, 159 (1 hour), 384 (48 hours), and 110 clones (96 hours) were selected and sequenced. BLASTX searches each cDNA library against Genbank revealed 15, 50, and 22 unique sequences at 1, 48, and 96hours after inoculation, respectively. Most highly represented ESTs at 1 hour after inoculation were photosynthesis-and senescence-related genes such as light harvesting chlorophyll a/b binding protein, ribulose-bishosphate carboxylase/oxyganase, and senescence-associated protein. Cytochrome P450-like TATA box binding proteins and manganese superoxide dismutase associated with the defense response, biotic and abiotic stresses were differentially expressed at 1 hour after inoculation. Although more than 50% of ESTs at 1 and 48 hours after inoculation were also associated with photosynthesis- and carbon fixation-related genes, defense-related genes were annotated as 14 clones at 48 hours after inoculation. Six ESTs associated with pathogen-defense and biotic and abiotic stresses were expressed only at 48 hours after inoculation. At 96 hours after inoculation, seven ESTs were involved in defense-response and biotic and abiotic stresses, and three out of the seven ESTs such as cyclophilin, F-box family, and leucine-rich repeat receptor-like kinase (LRR-RLK) were uniquely expressed. Quantitative real-time polymerase chain reaction analysis revealed that the transcripts for aldo-keto reductase (AKR) and LRR-RLK were highly expressed in the incompatible interaction. AKR gene was more highly expressed in resistant cultivar ‘Hwangsilri’ and moderately susceptible one ‘Gamcheonbae’ than in other cultivars. LRR-RLK showed differentially higher expression pattern in inoculated than in uninoculated leaves of susceptible cultivars including ‘Gamcheonbae’ and ‘Hwangkeumbae’. The ARK and LRRRLK genes were found to be associated with plant defense mechanism. However, more detailed further study using transformants introgressed these genes is required to understand how these genes are expressed and regulated by infection with V. nashicola.