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유압관로내 원통형 초크의 분류영역에서 맥동유동의 거동과 유동특성에 관한 연구
배신철,모양우,Moh, Y. W. 대한기계학회 1995 대한기계학회논문집 Vol.19 No.11
Cylindrical chokes are used widely as components of hydraulic equipments. The dynamic characteristics between flowrate and pressure drop through the cylindrical chokes were discussed by the frequency characteristics of the chokes. It was assumed no pressure recovery occurred near the downstream of the choke. The pulsating jetflow from the outlet of cylindrical chokes show very complex behaviours which are quite different from the steady jet flow but it's not clarified quantitatively. In order to utilize the chokes as a flowmeter, it is indispensable to discuss the estimation of the dynamics of pressure drop in the downstream jetflow region of cylindrical chokes. In this experimental study, it is clarified that the reattachment length depended on pressure wave is compared with it depended on velocity wave. A pulsating flow is verified by visualization method. In the present study, the flow characteristic variables of laminar pulsating flow are investigated analytically and experimentally in a circular pipe. Characteristic parameters of the ratios of inertia(.PHI.$_{t,1}$) and viscous(.PHI.$_{z,1}$) term to pressure term are introduced to describe the flow pattern of laminar pulsating flow. flow.low.
연속파형 Nd:YAG 레이저에 의한 인코넬 600의 용접 공정변수의 연구
배신철,유영태,송성욱,신호준,나기대 朝鮮大學校 機械技術硏究所 2004 機械技術硏究 Vol.7 No.1
The laser welding process is one of the most advanced manufacturing technologies owing to its high speed and deep penetration. Welding characteristics of Inconel 600 Alloy using a continuous wave Nd:YAG laser are experimentally investigated. The major process parameters studied in the present laser welding experiment were position of focus, laser power, travel speed. The gap and offset maintained as small as possible. Optical microscope were used to investigate the microstructures of the welded zone. The follow conclusions can be drawn the laser power and travel speed have a pronounced effect the fusion zone size and shape.
배신철,김명윤,Bae, Sin-Cheol,Kim, Myeong-Yun 대한기계학회 1997 大韓機械學會論文集B Vol.21 No.2
The theoretical model for the transient probe method is the modified Jaeger model which is used perfect line source theory. The transient probe technique has been developed for the simultaneous determination of thermal conductivity, diffusivity and volumetric heat capacity of liquids. The Levenberg-Marquardt iteration method is adapted to obtain thermal property within nonlinear range. Experimental results of liquids were found to agree well with recommended thermal property data.
도열병균(Magnaporthe grisea)의 Ribosomal DNA의 ITS II 부위 핵산 염기서열을 이용한 균주간 근연관계 비교
배신철,이신우,이인구,예완해,류진창 한국식물병리학회 1996 Plant Pathology Journal Vol.12 No.1
벼도열병균 14개 균주와 벼 이외 화본과 식물 도열병균 12개 균주를 대상으로 rDNA의 ITS II 부위를 증폭하여 그들의 핵산 구조 차이를 분석함으로 도열병균 균주간 분류를 시도하였다. 5.8S rDNA의 3`-말단 부위와 28S rDNA의 5`-말단 부위의 sequence 중 5`-CCCGGGAATTCGCATCGATCGATCGAATGAAGA-ACGCAGC-3`와 5`-CCCGGGATCCTCCGCTTATT-GATATGC-3`를 이용하여 PCR 증폭을 하였을 때 벼도열병균 14개 균주는 동일한 길이의 단일 밴드를 형성하였으며 벼 이외 화본과 식물 도열병균에서는 레드톱 식물로부터 분리한 도열병균만이 나머지 균주보다 38bp가 더 큰 길이를 가진 밴드를 형성하였다. PCR로 증폭된 DNA를 HaeIII와 MspI 제한효소로 절단하였을 때 벼도열병균 레이스간에는 제한효소 절단에 의한 전기영동 밴드 형태 차이를 관찰할 수 없었으나, 벼 이외 화본과 식물 도열병균 12개 균주는 3군으로 구분할 수 있었다. 벼도열병균 90=054와 강아지풀에서 분리한 도열병균 G90-5, 기장에서 분리한 G88-4, 바랭이에서 분리한 G88-5 그리고 레드톱에서 분리한 RT 균주의 ITS II 부위의 DNA 염기서열 비교 분석에 의하면 G88-4와는 다른 HaeIII와 MspI 제한효소 위치를 가지고 있었기에 제한효소 절단에 의한 전기영동 형태가 상이하였다. 또한 RT균주는 HaeIII와 MspI위치가 존재하지 않았다.
주파수의 변화에 따른 원형관로내 층류맥동유동의 속도와 압력의 분포
배신철,모양우,Bae, S.C.,Mo, Y.W. 대한설비공학회 1997 설비공학 논문집 Vol.9 No.4
In this paper, the fundamental equations are developed for the pulsatile laminar flow generated by changing the oscillatory flow with $0{\leq}f{\leq}48Hz$ into a steady one with $0{\leq}Re{\leq}2500$ in a rigid circular pipe. Analytical solutions for the wave propagation factor k, the axial distributions of cross-sectional mean velocity $u_m$ and pressure p are schematically derived and confirmed experimentally. The axial distributions of centerline velocity and pressure were measured by using Pitot-static tubes and strain gauge type pressure transducers, respectively. The cross-sectional mean velocity was calculated from the centerline velocity by applying the parabolic distribution of the laminar flow and it was confirmed by using the ultrasonic flowmeter. It was found that the axial distributions of cross-sectional mean velocity and pressure agree well with theoretical ones and depend only on the Reynolds number Re and angular velocity $\omega$.
대두 철분결합단백질 유전자 발현 형질전환 감자의 감자무름병 방어 증진효과
배신철,여윤수,허성기,황덕주,변명옥,고승주,Bae, Shin-Chul,Yeo, Yun-Soo,Heu, Sung-Gi,Hwang, Duk-Ju,Byun, Myung-Ok,Go, Seung-Joo 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.4
Ferritin is ubiquitous in bacteria, animals and plants. Ferritin is thought to play two main roles in living cells to provide iron for the synthesis of iron protein such as ferretoxin and cytochromes and to prevent damage from radicals produced by iron/dioxygen interaction. To enhance the resistance of potato to Erwinia carotovora, the soybean ferritin gene was introduced into the potato either under CaMV 35S or hsr203J promoter. Potato transgenic plants were screened by PCR analysis using specific primers to the ferritin gene. Expression of ferritin gene under CaMV 35S and hsr203J promoter in potato transgenic plants was confirmed by northern blot analysis. hsr203J promoter known to pathogen inducible in tobacco drives the induction upon Phytophthora infestan in potato and the transcript level of ferritin gene was extremely high after 24 hours post inoculation. One of transformants under CaMV 35S promoter was increased 2.5 fold than untransformant. Each one of transgenic potato containing gene promoter CaMV 35S and hsr203J-ferrtin fusion exhibited tolerance against potato soft rot. 식물의 항산화력을 증진하여 식물병원균 저항성 작물을 개발하고자, 철분 결합 단백질인 대두의 ferritin 유전자를 CaMV 35S와 hsr203J promoter에 연결하여 감자에 형질전환하였다. PCR및 Northern분석에 의한 형질전환 감자에 ferritin 유전자가 존재하는 것과 이들 유전자 식물체내에서 발현되는 것을 확인하였다. ferritin 유전자를 담배 유래 병원균 특이 발현promoter인 hsr203J와 연결하여 획득된 형질전환 감자 식물체는 감자역병균 접종 후 24시간대에서 전사체 발현량이 가장 많았으며 그 후 줄어드는 경향을 나타내었다. 유전자 도입이 확인된 형질전환체 감자괴경의 철분 함량은 CaMV 35S와 ferritin 유전자 도입 형질전환체가 2.5배, hsr203J promoter와 ferritin 유전자 도입 형질전환체가 1.5배 각각 증가하는 것으로 나타냈다. 또한 이들 형질전환체는 감자 무름병균에 대한 저항성 증진효과를 관찰할 수 있었다.