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박종수,윤민호,최우영,Park, Jong-Soo,Yoon, Min-Ho,Choi, Woo-Young Institute of Agricultural Science 1997 Korean Journal of Agricultural Science Vol.24 No.2
The prcx.iuction of extracellular 1,4-${\beta}$-glucanase by Cellulomonas sp. CS1-1 on microcrystalline cellulose, sigmacell was maximal after 5-day cultivation as 280 units/mL, which was three times higher than the level produced on carboxymethyl cellulose. A carboxymethyl cellulase containing the carbohydrate of 8.2% was purified from the culture filtrate by successive procedures of column chromatographies. Purification factor was calculated as 22-folds with the specific carboxymethyl cellulase activity of 31.9 units/mg. The molecular weight and isoelectric point of the purified enzyme were 54,000 and pI 5.4, respectively. The optimal pH and temperature were 6.0 and $45^{\circ}C$, and the enzyme was stable between pH 6.5 and 7.5 and below $50^{\circ}C$. The estimated Km and Vmax were 10 mg/mL and $6.25{\mu}mol/min$ for carboxymethyl cellulose and 30.3 mg/mL and $2.85{\mu}mol/min$ for sigmacell, respectively. The enzyme was partially inhibited by $Ag^+$, $Zn^{+{+}}$, $Fe^{+{+}}$ and EDTA, while completely inhibited by $Cd^{+{+}}$ and $Hg^{+{+}}$ at 1 mM concentration. 섬유소분해세균 Cellulomonas sp. CS1-1을 미소결정성 섬유소(sigmacell)에서 배양하면서 생육 및 1,4-${\beta}$-glucanase의 생산량을 조사하였다. 생균수는 배양 1일만에 최대이었지만 1,4-${\beta}$-glucanase의 활성도는 CMC 당화력으로 280 units/mL, 점도저하력으로 $3{\times}10^3units/mL$으로서 배양 5일째 최대이었으며, 이것은 가용성기질에 비하여 약 3배 높은 수준이었다. 또한 배양여액으로 부터 황산암모늄침전, Ultro-gel Ac54 겔여과, DEAE-Sephadex A50 이온교환크로마토그래피, 그리고 Con A-Sepharose 4B 친화성크로마토그래피의 방법으로 CMCase의 한분획을 순수정제하였다, 정제한 CMCase는 8.2%의 탄수화물을 함유하는 당단백질이었으며 그 정제도는 배양여액에 비해 CMC 당화력으로 22배 증가한 것이었다. 분자량은 54,000 그리고 둥전점은 pI 5.4 이었으며, 작용최적 pH 및 온도는 각각 pH 6.0 과 $45^{\circ}C$ 이었다. 정제효소의 CMC에 대한 Michaelis 상수 및 최대반응속도는 10 mg/mL, $6.25{\mu}mol/min$, 그리고 sigmacell에 대해서는 각각 30.3 mg/mL, $2.85{\mu}mol/min$으로 계산되었다 효소의 활성도는 $Ag^+$, $Zn^{+{+}}$, $Fe^{+{+}}$ 그리고 EDTA에 의해 부분적으로 저해되었고, 1mM의 $Cd^{+{+}}$와 $Hg^{+{+}}$ 이온에 의해서는 완전히 저해되었다.
박종수 ( Jong Soo Park ),이장상 ( Jang Sang Lee ),김민경 ( Min Kyoung Kim ) 한국축산경영학회,농업정책학회(구 한국축산경영학회) 2011 농업경영정책연구 Vol.38 No.3
In this study, we surveyed and analyzed farmers` awareness of the pork checkoff program and the associated activities in the program to promote pork consumption as well as their acceptance to the allocated fee (600won/head). Although farmers recognize the importance of the checkoff program in promoting pork consumption, some discrepancies in attitudes toward the program are found between board members and producers. Therefore, it is highly recommended to more actively publicize the importance of the pork checkoff program to producers. We concluded this study by suggesting several solutions, derived from our findings, to improve the pork checkoff program.
퇴적물내 coprostanol을 이용한 진해만의 도시하수오염 평가
최민규 ( Min Kyu Choi ),문효방 ( Hyo Bang Moon ),김상수 ( Sang Soo Kim ),박종수 ( Jong Soo Park ) 한국수산과학회 2005 한국수산과학회지 Vol.38 No.2
N/A Surface sediment samples from 31 stations in Jinhae Bay were analyzed to evaluate the pollution by sewage from the spatial distribution of the fecal sterol, coprostanol. The sums of eight sterols (coprostanol, epicholestanol, epicoprostanol, cholesterol. cholestanol, brassicasterol, stigmasterol and β-sitosterol) were in the range of 2,703-27,154 ng/g dry weight. The concentrations of the coprostanol in the sediments, which is a good tracer of sewage-derived organic contamination, were in the range of 76-3,964 ng/g dry weight. The levels of coprostanol were much higher (almost one order of magnitude) at stations close to the big cities such as Masan and Jinhae. However, those levels were comparable to or lower than those previously reported in other foreign locations. Some ratios of coprostanol to other sterols and multivariate analysis could provide us with the information on the origin of the sterols being from sewage, plankton, and/or benthos. Those showed sewage dominance at the stations near the cities Masan and Jinhae, plankton dominance at the center of Jinhae Bay and mainly marine fauna origin at the mouth of Jinhae Bay. These results suggest that the contents of sterols and some ratios of these components are quite powerful tracer for the detection of the origin of the organic contaminants in the coastal environments.
허승,박종수,안경호,이윤,최옥인,임동현,황운기,이승민,김평중,방현우,Heo, Seung,Park, Jong-Soo,An, Kyoung-Ho,Lee, Yoon,Choi, Ok-In,Lim, Dong-Hyun,Hwang, Woon-Ki,Lee, Seung-Min,Kim, Pyoung-Joong,Bang, Hyun-Woo 해양환경안전학회 2010 해양환경안전학회지 Vol.16 No.3
황해의 해양환경, 어업자원 및 증양식에 대한 연구 및 개발을 담당하고 있는 국립수산과학원 서해수산연구소에서 해양생태계 보전의 일환으로 황해의 해양환경에 대한 다양한 모니터링을 실시하고 있다. 현재 서해수산연구소가 황해에서 수행 중인 모니터링은 연안정지관측, 서해해양조사, 서해어장환경모니터링, 국가해양환경측정망, 유해적조모니터링, 한중황해환경모니터링조사 및 해파리모니터링 등이 있다. 이러한 모니터링 결과들은 중요 어족의 어장형성 해역에 대한 기초자료를 제공하여 연근해 어업생산성을 제고하고, 기후변동에 따른 향후 수산자원 변동 예측을 위한 기초자료로 활용되고, 한반도 주변해역의 체계적인 해양과학자료를 제공하여 수산업, 해양환경보전, 해양레저 등 한반도 주변해역의 부가가치 창출에 기여하고 있으며, 연안어장의 지속적 생산을 유지하기 위한 어장환경 관리 및 보전정책 수립을 위한 국가 기본 자료로 활용하고 있다. The West Sea Fisheries Research Institute of National Fisheries Research and Development Institute which is in charge of research on marine environment, fisheries resources and aquaculture carries out various monitoring projects with an aim of marine ecosystem conservation. The monitoring projects include costal oceanographic observation, serial oceanographic observation, fishing ground monitoring, national marine environmental monitoring, harmful algal bloom monitoring, Korea-China joint monitoring on the Yellow Sea and jellyfish monitoring. The monitoring produces basic data on fishing ground locations of main fishery species to improve fishery productivity. The data are also used to estimate variations in fisheries resources caused by climate change and to set up the policy for creating economic value from fishery, marine environmental conservation and marine leisure activities and conserving/controlling the marine environment for the sustainable production in the fishing ground.