월악산국립공원의 부착규조 분포와 유기오탁지수 (DAIpo)에 의한 수질평가

박정원(Jung-Won Park) 국립공원연구원 2010 국립공원연구지 Vol.1 No.2

본 연구는 2006년에 월악산국립공원 내에 부착규조류 분포와 유기오탁지수에 의한 수질 평가이다. 동정된 부착규조류는 138종류였고, 우점종은 Achnanthes sp. Achnanthes alteragracillima, Achnanthes convergens, Achnanthes minutissima, Cocconeis placentula var. lineata, Cymbella affinis, Cymbella silesiaca, Gomphonema minutum, Reimeria sinuata 등이었다. DAIpo 값은 62.3에서 99.9의 범위로, 매우 좋음에서 좋음으로 평가되었다. To investigated the epilithic diatoms community and an assesment of water quality by DAIpo(diatom assemblage index of organic water pollution), the samplings were collected from fourteen sampling stations on April, May, August, September and October 2006 in Woraksan National Park. The total 138 taxa were identified from this study and dominant species were Achnanthes sp. Achnanthes alteragracillima, Achnanthes convergens, Achnanthes minutissima, Cocconeis placentula var. lineata, Cymbella affinis, Cymbella silesiaca, Gomphonema minutum, Reimeria sinuata. The variations of DAIpo values was ranged from 62.3 to 99.9 and the water quality was estimated between very good and good.

지상라이다를 이용한 미기록 외래종 갯쥐꼬리풀(Spartina Alterniflora)의 분포특성과 관리방안 연구 -다도해 해상국립공원 진도 남동리 해안을 사례로-

박정원 ( Jung Won Park ),김하송 ( Ha Song Kim ),장성건 ( Seong Geon Jang ),천숙진 ( Sook Jin Chun ),육관수 ( Kwan Soo Yuk ) 한국도서학회 2015 韓國島嶼硏究 Vol.27 No.3

갯쥐꼬리풀(Spartina alterniflora)은 북미 대서양 연안 뉴펀들랜드, 캐나다 남쪽에서 아르헨티나 북부의 갯벌이 원산이며, 생장이 강한 특성으로 세계의 기수지역과 해안에서 생태공학적 용도로 도입된 바 있다. 그러나 매우 빠르게 갯벌을 피복하여 자생종의 생장을 방해하고, 염습지 종다양성을 감소 시켰고, 결과적으로 과다생육으로 갯벌 무척추동물의 서식지를 잠식해 사라지게 하여 무척추동물을 먹이로 하는 조류 또한 생태계 내에서 감소키는 등 많은 환경문제를 야기 시켰다. 우리나라에서는 2008년 전후에 다도해해상국립공원에 유입된 것으로 판단된다. 현재 다도해해상국립공원 남동리 해안에서 분포하고 있는 갯쥐꼬리풀(Spartina alterniflora)군락은 습지내 총 6개의 염생식물 군락면적(8,487.7㎡) 중 75.34%의 비율(6,394.86㎡)로 분포하고 있다. 또한 2008년에 11.54㎡ 이던 분포 면적이 2015년 현재 약 550배가 늘어난 6,394.22㎡로 조사되었고 생육지 고도 범위인 0.69m~1.1m를 요인 값으로 두고 GIS 분석한 하였다. 그 결과 앞으로 확산 될 예상 면적은 12,584㎡이며 현재보다 약 6,189.14㎡가 늘어날 것으로 예측되며 우리나라에서도 역시 확산이 빠르고 넓게 늘어날 것으로 판단된다. 향후 계속 분포역이 확대 될 것으로 예상하고 관리방안을 위하여 시범 연구를 실시하였다. 갯쥐꼬리풀군락 중 일부를 첫 번째는 뿌리째 뽑아내기와 두 번째 지상부 제거 후 포장덮기 방법 등 국외사례의 문헌을 바탕으로 방재를 실시하였으나 전체 면적을 방재하기에는 많은 인력과 시간 그리고 예산이 소요되므로 다각적인 방안을 강구하여야 한다. Spartina alterniflora is native to the Atlantic coast from Newfoundland, the south of Canada and northern Argentina. There it forms a dominant part of brackish coastal saltmarshes. Due to its strong growth, the Spartina alternifolra was introduced to the rider area and coast as a use for ecological engineering. The Spartina alterniflora community can crowd out native species, reduce the biodiversity of saltmarsh and alter the environment. As a result, the invertebrates living in mud flats disappeared due to the encroaching of Spartina alterniflora, and the birds which eat off invertebrates went along as well. The Spartina alterniflora was first introduced in South Korea in 2008 and is known to have flowed in the Dadohaehaesang National Park. Compared to 2008, in 2015 the distribution area of the Spartina alterniflora has increased 550 times. (11.54㎡ in 2008 to 6,394.22㎡ in 2015). The large increase of Spartina alterniflora calls for urgent management and additionary study on the subject. Thus, this study analyzed the geo-spatial information on the Spartina alterniflora such as its distribution area, the height of habitats and expected expansion area, and can act as the preliminary data for management.

급성폐쇄각발작의 과거력을 가진 백내장수술 시 수정체낭내확장고리의 효과

박정원,정현호,박상우.Jung Won Park. MD. Hyun Ho Jung. MD. Sang Woo Park. MD. PhD 대한안과학회 2013 대한안과학회지 Vol.54 No.9

Purpose: To evaluate the effect of a capsular tension ring (CTR) on the change in postoperative refractive errors and anterior chamber depth after cataract surgery in patients with a history of acute primary angle closure (APAC). Methods: We retrospectively reviewed medical records of 45 patients who underwent cataract surgery with a history of APAC. Forty five eyes were divided into 2 groups based on whether a CTR was implanted (n = 21) or not (n = 24). Spherical equivalent (SE) refractive errors were compared between the 2 groups for 12 months postoperatively. The postoperative anterior chamber depth (ACD) and volume (ACV) were compared between the 2 groups. Results: SE refractive errors in the CTR group were -0.59 ± 1.08 D at 6 months and -0.61 ± 1.04 D at 12 months postoperatively. In the non-CTR group, -0.64 ± 1.30 D, at 6 months and -0.67 ± 1.19 D at 12 months postoperatively. There was no statistically significant difference between 2 groups (p = 0.89, p = 0.84) and postoperative SE refraction shifted to myopic refractive power in both groups (p < 0.01). The postoperative ACD and ACV also showed no difference between the 2 groups (p = 0.13, p = 0.47). Conclusions: The postoperative ACD, ACV and myopic shift in patients with a history of APAC demonstrated no significant difference between CTR-implanted and non-CTR-implanted patient groups.

금호강의 Scenedesmus ( 녹조강 , 녹색소구체목 ) 속의 개체군 변화

박정원,정준 ( Chung Won Park,Jun Chung ) 한국하천호수학회 1996 생태와 환경 Vol.29 No.1

To investigate the ecological variation of the genus Scenedesmus populations, the survey had been carried out at nine sampling stations in the Kumho river in every two month interval from October, 1990 to August, 1993. The total number of taxa collected from this area were classified into 38 species, 6 varities, 2 formae, and also they appeared 21∼39 taxa at each station. Relationship between environmental factors and standing crops, population density of the Scenedesmus was high at water temperature 9.7∼27℃, EC average 311∼614 S/cm, BOD average 2.47∼12.72 ppm. The important species investigated as followes: S. acutus, S. communis, S. opoliensis, S. serratus, S. spinosus. The subgenus Acutodesmus and Scenedesmus were high occupancy percentage of standing crops between α-oligosaprobic and α-mesosaprobic. But the subgenus Desmodesmus was high between β-mesosaprobic and polysaprobic. By the results obtained this study, the genus Scenedesmus populations distributed in the different environment. And also the subgenus Acutodemsus(including the subgenus Scenedesmus) and the subgenus Desmodesmus showed to be the ecological diversity each other.

Proteome Analysis of Drosophila melanogaster Used 2-DE and MALDI- TOF-MS

박정원,차재영,송재영,김희규,김범규,전병삼,Park Jeong-Won,Cha Jae-Young,Song Jae-Young,Kim Hee-Kyu,Kim Beom-Kyu,Jeon Beong-Sam Korean Society of Life Science 2005 생명과학회지 Vol.15 No.3

초파리는 유전학, 발달 생물학, 행동 유전학, 노화 연구에 이르기까지 수많은 연구에 이용 되어져 왔다. 최근 초파리 전체 유전자의 염기서열이 보고되었으며, 유전자의 기능 분석과 발현 단백질에 대한 연구가 진행되고 있다. 본 연구에서는 야생형 초파리에서 추출한 단백질을 이차원 전기영동을 통해 400여개 이상의 단백질 스폿으로 분리하였으며, 각 스폿을 적출하여 트립신으로 처리하여 얻어진 펩타이드 단편을 MALDI-TOF-MS를 이용한 펩타이드 지문 검색으로 질량을 측정하였다. 측정된 질량을 초파리 데이터 베이스를 이용하여 분리한 단백질을 동정함으로써 59개의 유전자에서 발현되는 65개의 단백질 스폿을 동정하였다. 이러한 결과는 향후의 발생단계, 외부 자극, 노화 등에 관련되는 특이적 단백질 연구의 기초 자료로 활용될 수 있을 것이다. With the completely discovery of the Drosophila genome sequence, the next great challenge is to extract its biological information by systematic expression and to perform functional analysis of the gene. Here we reported a proteome analysis of D. melanogaster with two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS). The cell extracts of D. melanogaster, $200{\mu}g$ were resolved to more than 400 silver-stained spots by 2-DE. The most abundant protein spots were ranged from 4.0-7.5 of pI and from 15-90 kDa of molecular weight. The excised spots were destained and in-gel digested by trypsin. The masses of the resulting peptide mixtures were measured by MALDI-TOF-MS. Identified proteins were compared with measured peptide mass and a dynamic peptide searching database which is accessible via the internet. The results revealed that identified proteins were produced by 59 genes derived from 65 protein spots.

초등학생의 식습관과 인공지능 푸드스캐너로 측정한 학교급식 섭취의 연관성 연구

박정원,손금희,우사라,박경희,임현정,Park, Jungwon,Son, Kumhee,Woo, Sarah,Park, Kyung Hee,Lim, Hyunjung 대한영양사협회 2022 대한영양사협회 학술지 Vol.28 No.4

The lower elementary school grades are an important period in which eating habits are formed. We examined the relationship between eating habits and school meal intake in the lower grades of an elementary school in Seoul. The eating habits were investigated using the Nutrition Quotient (NQ) for children. The school meal intake rates and preferred menus were obtained by automatically scanning the plate before and after meals using an artificial intelligence food scanner. The average school meal intake rate for the 347 subjects was 68.5±12.2%, and the nutrient intakes through the school meals were 353.5±70.0 kcal of energy, 51.8±10.2 g of carbohydrates, 14.6±3.1 g of proteins, 10.3±2.3 g of fats, 87.0±20.0 mg of calcium, and 1.8±0.4 mg of iron. The preferred menus were rice, grilled food, and dairy products, and non-preferred menus were salad, beverages, and stewed food. The eating habits that showed a positive correlation with the school meal intake rate were 'Diverse side dishes (r=0.332, P<0.001)', 'Vegetable side dishes (r=0.166, P<0.01)', 'Kimchi side dish (r=0.230, P<0.001)' and 'Less TV watching and computer game time (r=0.105, P<0.05)'. The NQ score also showed a positive correlation with the rate of school meal intake (r=0.216, P<0.001). The balance score was positively correlated with fruit (r=0.192, P<0.001), and the diversity score had the highest positive correlation with Kimchi (r=0.362, P<0.001). The regularity score was positively correlated with fried food (r=0.114, P<0.05). In conclusion, it was found that elementary school students in the lower grades had a higher school meal intake rate when their eating habits included eating side dishes evenly, and consuming vegetable side dishes and Kimchi.

금호강의 (琴湖江) 식물플랑크톤 군집 (群集) 구조

박정원,김용재,정준 ( Chung Won Park,Yong Jae Kim,Jun Chung ) 한국하천호수학회 1995 생태와 환경 Vol.28 No.1

The species composition and variation of phytoplankton community were investigated in the Kumho River from October 1990 to August 1991. The total 203 taxa, including Chlorophyceae, Euglenophyceae, Cyanophyceae, Dinophyceae, Chrysophyceae, were indentified. Among them, the dominant species are Chlamydomonas angulosa, Monoraphidium contortum, Pandorina morum, Euglena limnophila, Euglena sp., Scenedesmus acutus, Scenedesmus quadricauda, Scenedesmus spinosus. The standing crops of the phytoplankton community ranged between 290cells/ml at 1 station in March, 1991 and 74280cells/ml at 5 station in June, 1991. Euglenophyceae attained their maximum occurrence during autumn and spring in the downstream. The diversity index and the dominant index were ranged 0.3-1.43 and 0.05-0.99, respectively. According to environmental factors and phytoplankton community, it suggests that the water quality of the Kumho River was ranged from oligotrophic state to mesotrophic state at st. 1 and st. 2, mesotrophic state to eutrophic state at st. 3 and st. 4, hypertrophic state at st. 5.

금호강에서 유글레나강의 개체군 변화

박정원,정준 ( Chung Won Park,Jun Chung ) 한국하천호수학회 1996 생태와 환경 Vol.29 No.2

To investigate the population dynamics of Euglenophyceae, the survey had been carried out bimonthly at nine stations from October, 1991 to August, 1993. The total 44 taxa collected from this study were classified into 39 texa(including 5 varities), 6 genera, 2 families, 1 order and a total standing crops of Euglenophyceae varied from minimum of 3,665 cells/ml to maximum of 165,920 cells/ml. The important species based on abundance investigated as followes : E. proxima, E. viridis, L. texta, T. volvocina. The standing crops of Euglenophyceae showed maximum value during winter at downstrean and the significant factors for population development of Euglenophyceae were DO, BOD, phosphate, ammonia. The population density of Euglenophyceae peaked especially low water temperature

수동밸브와 자동밸브

박정원,Park, Jeong-Won 한국설비기술협회 1996 설비 : 공조ㆍ냉동ㆍ위생 Vol.13 No.11

상생경영-KT편

박정원,Park, Jeong-Won 벤처기업협회 2008 벤처다이제스트 Vol.119 No.-