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박영석,이재홍,Park, Young-seok,Lee, Jae-hong 대한수의학회 1992 大韓獸醫學會誌 Vol.32 No.3
In order to study on the Sertoli cell, we attempt have been made to measure the average number of each germ cells per Sertoli cell on the 12 stages of cycle in matured korean Jindo dog. The fine structure of Sertoli cell junctional specialization was studied with electron microscope. The results were summarized as follows; 1. The average number of various germ cells associated with Sertoli cell was 9.77 to 13. 80 through stages of cycle and the total average number was 11.62. 2. Sertoli-Sertoli cell junctional specialization was present in seminiferous epilthelium, and Sertoli-spermatid cell junctional specialization rose from stage 8 spermatid, persisted to step 13 spermatid and then disappeared. The structure of Sedoli-spermatid cell juncticnal specialization was not similar to that of Sertoli cxlls. 3. Just after spermiation, free-surface of Sertoli-spermatid cell junctional specialization was replaced by Sertoli cell cytoplasm with tubulobulbar complex at the neiglaboring region observed. 4. The Sertoli cell process was located within the cytoplasm of late stage spermatids. Some membranes of residual body and spermatid cytoplasm partly disappeared, resulting in opening of the cytoplasm of spermatid into that Sertoli cell. This fact suggested that spermatid cytoplasm was partly eliminated.
진도견 정자형성계 세포들의 Lectin-binding patterns
박영석,이성호,Park, Young-seok,Lee, Seong-ho 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3
The lectin-binding patterns in the testis of the sexually matured Jindo dog were investigated to study the distribution of glycoconjugates in the seminiferous tubule under light and transmission electron microscopy. Positive reactions to Wheat germ agglutinin(WGA) and Dolichos biflorus agglutinin (DBA) were observed in the Sertoli cell and in the residual body of spermatid with a stronger reaction in the Sertoli cell to the lectins than in the residual body. Strong reactions to Soybean agglutinin(SBA) and Peanut agglutinin(PNA) were observed in the acrosome vesicles of the Golgi- and cap-phase spermatid, while a moderate reaction was observed in the acrosome-phase, maturation-phase spermatid and the residual body. The acrosome area of the spermatid reacted intensively to Griffonia simplicifolia agglutinin( GS-I) when the cell was in the acrosome-phase and maturation-phase, and the same reaction to the GS-I was observed in the residual body. However, the seminiferous tubule did not react to Ulex europeus agglutinin I(UEA-I). The gold-labelling of the Sertoli cells with DBA resulted in positive reactions of the Sertoli cell column and processes when observed under the electron microscopy, while the Golgi-, cap- and acrosome-phase spermatids reacted positively to SBA in the peripheral low-dense area of the acrosome vesicle of spermatid. Based on these results, we concluded that differences in the lectin-binding pattern of the seminiferous tubules were recognized in the Jindo dog compared to other animals.
진도견 정세관의 정세포와 Sertoli 세포내 glycogen의 분포
박영석,이성호,Park, Young-seok,Lee, Seong-ho 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3
In an effort to obtain basic data of carbohydrate metabolism during spermiogenesis of the sexually-matured Jindo dog, the glycogen distribution in the testis was investigated by light and transmission electron microscopy. Periodic acid thiocarbohydrazide silver proteinate physical development(PA-TCH-SP-PD) staining method provided better results in the detection of glycogen granules from Sertoli cells and germ cells than the periodic acid schiff(PAS) staining method did. Pre-treatment of the tissue sections with ${\alpha}$-amylase elicited a significant decrease in PA-TCH-SP-PD stained granules, which suggested that the stained granules were of glycogen origin. High concentration of the glycogen granules were observed in the Sertoli cells, especially in its column, sheet-like processes, club-like processes, and tubular processes. The glycogen granules were unevenly distributed in some Sertoli cell columns. These results strongly indicated that the Sertoli cells of Jindo dogs showed vigorous activity of carbohydrate metabolism.
진도견(珍島犬)의 정자형성(精子形成)과 Sertoli세포(細胞) 특수(特殊) 연접부(連接部)의 미세구조(微細構造) I. 진도견(珍島犬)의 정자형성(精子形成)에 관한 연구(硏究)
박영석,이재홍,Park, Young-seok,Lee, Jae-hong 대한수의학회 1992 大韓獸醫學會誌 Vol.32 No.3
Classification of the cycle of seminiferous epithelia into 12 stages by the morphological changes in acrosomal system and evaluation of the relative frequency of stages and the cell association were histologically performed in the mature Korean native Jin-do dogs. The results were summarized as follows; 1. The minimum number of type A spermatogonia averaged 1.01 at stages I, while maximum number averaged 2.47 at stages XII. Some type A spermatogonia divided at stage XII to produce the type intermediate(IN) spermatogonia at the following stage I. The type IN spermatogonia divided at stage IV to produce the type B spermatogonia at stage V. 2. The type B spermatogonia divided at stage VI to produce the preleptotene primary spermatocytes at stage VII. The secondary spermatocytes observed at stage XII. The secondary spermatocytes observed at stage XII divided to give rise to the round spermatids at the following stage I. The numbers of the first spermatocytes and spermatids were almost constant, respectively, through all the cycles of seminiferous epithelium. 3. The acrosomal vesicle was invaginated to occupy one third to half of spermatid nucleus at the cap phase, which was different from that of rodent and ruminant spermatid nuclei. 4. The relative frequencies of stages I to XII of seminiferous epithelia cycle were 10.34, 4.84, 5.03, 8.22, 10.86, 6.63, 6.42, 18.88, 10.17, 6.18, 7.62% and 4.81%, respectively.
VR의 현실효과 그리고 가상과 실재의 관계: SF영화의 미래적 비전을 중심으로
박영석 ( Park Young Seok ),문재철 ( Moon Jae Cheol ) 한양대학교 현대영화연구소 2022 현대영화연구 Vol.18 No.2
오늘날 VR은 기술박람회를 벗어나 문화예술 콘텐츠의 영역으로 진입하는 중이다. 아직까지는 특정한 주제의식과 담론을 내재하기보다는 새로운 기술에 대한 어트랙션을 유발하는 체험형 콘텐츠에 가깝지만, 앞으로 미디어 기술의 정교한 발전이 계속된다면 콘텐츠의 질적 측면에 더 가치를 두는 방향으로 전환이 일어날 가능성이 높다. 이러한 관점에서, 본 논문은 VR기술과 콘텐츠 수용 양상의 변화 및 파생효과에 대해 미래적 비전을 제시한다. 그 비전은 VR기술의 실질적 발전 방향에 대한 예견은 물론이고 SF영화의 상상적 미디어를 경유하는 장르적 도약까지 포함하여 크게 두 단계로 구성된다. 첫 번째 비전은 VR의 기술적 완성도가 당연한 것으로 여겨지고 몰입과 상호작용성이 적절히 조화를 이루면서 미디어 장치가 투명해질 정도의 현실효과가 발생하는 단계이다. 사용자는 그 세계에 몰입하고 현존감을 느끼며 상호작용의 조작과 커뮤니케이션을 하는 적극적인 행위자가 될 수 있다. 이러한 기술적 조건의 파생효과로써 가상경제와 가상노동의 가능성을 고려할 수 있다. 두 번째 비전에는 가상현실 기술이 특이점을 넘어 근본적 전환을 맞이한다는 SF적 도약이 전제된다. SF영화에서 재현되는 것처럼 정신의 총체적 접속을 이루는 시뮬레이션 형식의 가상현실 기술이 가능해진다면, 사용자가 가상과 현실의 경계를 식별할 수 없고 가상과 실재의 경계가 무화되는 일이 벌어질 수 있다. 이 논문은 이러한 VR의 미래적 비전이 작동하기 위한 기술적 쟁점을 추출하고, 이때 사용자와 가상세계가 어떠한 존재론적 위상 변화를 맞이하는지 철학적으로 고찰한다. Today, VR is entering the realm of cultural and artistic content beyond the technology fair. So far, it is more like experiential content that induces attraction to new technologies rather than inherent specific topics and discourses. If sophisticated development of media technology continues in the future, there is a high possibility that a shift will occur in the direction of placing more value on the quality aspect of content. From this perspective, this paper aims to present a future vision for changes in VR technology and content acceptance modes, and its derivative effects. The vision will be largely composed of two stages, including not only a prediction of the actual development direction of VR technology, but also a genre leap through the imaginary media of sci-fi films. The first vision is the stage in which the technological perfection of VR is taken for granted, and immersion and interactivity are properly harmonized, and the reality effect occurs to the extent that the media device becomes transparent. Users can be immersed in the world, feel their presence, and become active actors who manipulate interaction and communicate. As a derivative effect of these technological conditions, the possibility of virtual economy and virtual labor can be considered. The second vision presupposes a sci-fi leap forward that VR technology will face a fundamental transition beyond its singularity. If VR technology in the form of a simulation that realizes a holistic connection of the mind becomes possible, as represented in sci-fi films, users cannot distinguish the boundary between virtual and reality, and the boundary between the virtual and the real may become blurred. This paper extracts the technical issues for the future vision of VR, and philosophically considers what kind of ontological change the user and the virtual world face at this time.
박영석(Young Seok Park),이윤정(Yun Jeung Lee),이기택(Ki Teak Lee) 한국식품영양과학회 2006 한국식품영양과학회지 Vol.35 No.10
Concentrations of formaldehyde and acetaldehyde were respectively analysed in forty-five alcoholic beverages obtained from the market. After derivatization with PFBHA, GC-ECD and GC-MSD were employed for analysis. The peak area of aldehyde oximes (derivatives with PFBHA) increased with the increasing ethanol content (5%, 10%, 15%, 20% and 40%). When three-point calibration curves for the selected ethanol concentration (5, 13, 21 and 40%, v/v) were studied, suitable linearity against ethanol concentration was observed only under 5, 13, and 21% (ethanol, v/v). After analysis, maximum content of formaldehyde (average of 0.272 ppm) and acetaldehyde (average of 15.262 ppm) among the observed 45 alcoholic beverages was found from whisk (2 species) while minimum content of formaldehyde (average of 0.009 ppm) and acetaldehyde (average of 0.805 ppm) was found from diluted soju (4 species).