중풍 임상 연구를 위한 항목 선정 및 정보관리 프로그램 개발

문진석,최선미,조기호,윤유식,Moon, Jin-Seok,Choi, Sun-Mi,Cho, Ki-Ho,Yoon, Yoo-Sik 한국한의학연구원 2005 한국한의학연구원논문집 Vol.11 No.1

In a field of oriental medicine, it is necessary to collect systemically clinical data and integrate. Input data items was decided, then categorized by the modules through discussion of the institute and hospitals. Items are information of patient, history, aspects of occurrence, pattern identification, prescriptions of herbal medicine, the results of biochemical serum examination, blood cell count, urine analysis, CT, MRI, Chest PA, EKG etc. Factors in oriental medicine are Sasang constitution, Stroke-Pattern-Identification(china), differentiation of cold and heat syndrome. This tool was constructed by using Microsoft-Access 2000 and Microsoft Visual Basic 6.0. Furthermore this web-based system could be applied to multi-center clinical investigation.

Transcript Profiling of Native Korean Grapevine Species Vitis flexuosa Exposed to Dehydration and Rehydration Treatment

문진석,허윤영,정성민,최윤정,남종철,박정관,고상욱 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.1

Vitis flexuosa, a native Korean wild grapevine species, is more tolerant to abiotic stress than cultivatedgrapevines, but little is known about the mechanism underlying this tolerance. To better characterize the responses ofV. flexuosa to drought stress and subsequent rehydration, we treated V. flexuosathe plants with four stress conditions:short- and long-term dehydration, rehydration immediately after long-term drought, and long-term rehydration afterlong-term drought. We examined gene expression profiles in response to these treatments using whole-genomemicroarray analysis at 12X coverage (using the NimbleGen protocol). We identified a 5,964 genes that were differentiallyexpressed between the stress treatments and the well-watered control. Gene ontology analysis showed that most ofthe differentially expressed genes (DEGs), regardless of treatment, participate in the metabolism of proteins or phosphatecontainingcompounds and function in the binding of metal ions, as kinases, or as phosphotransferases. Kyoto Encyclopediaof Genes and Genomes (KEGG) pathway analysis revealed that genes involved in secondary metabolite biosynthesiswere downregulated during dehydration but upregulated during rehydration. Among the DEGs encoding transcriptionfactors, genes in the MYB family were the most common differentially genes in all treatment groups. The NAC familywas the second most common group during dehydration, while the AP2/ERF family was the second most commonduring rehydration. These results will help elucidate the mechanisms underlying the response of V. flexuosa todrought stress and will be valuable for breeding drought-tolerant grapevines.

“주민자치는 실수와 경험 축적을 통해 발전한다”

문진석 한국자치학회 2020 월간 주민자치 Vol.103 No.-

한양방 임상연구 증례기록서의 온톨로지 구조 설계 및 웹기반 입력폼 자동 생성 알고리즘 개발

문진석,박경모,최선미 대한의료정보학회 2009 Healthcare Informatics Research Vol.15 No.1

Objective: This study aims to develop the web-interface creator, which automatically changes the Case Report Form(CRF) web page when the protocol developer in any clinical study revises the ontology of CRF. Methods: This study established the conceptual model of CRF on oriental and western medicine, and developed ontologies. A program was developed to produce online-based a input form through the parser that generates automatically HTML script from OWL. Results: The CRF applied in this study is a draft through consensus of experts for stroke research at the Korea Institute of Oriental Medicine. The ontology of CRF consisted of Label, ControlType and Value classes and hasControl, hasValue and hasSymptoms properties. The Label is the class of question items groups, so it could have CRF questionnaire instances. The ControlType is the class that expresses controls such as checkbox, text, etc in the HTML script. The Value class represents selections for each items. Finally, the HTML script was created by XSL transformation from the OWL script. Conclusion: Online-based automatic interface creation, which enables immediate coping with the continuous change in the medical knowledge system, assures reduced time requirement.

전문가 가중치 부여를 통한 비만변증설문지 적용

문진석,강병갑,강경원,심우진,신미숙,최선미,Moon, Jin-Seok,Kang, Byung-Kab,Kang, Kyung-Won,Shin, Woo-Jin,Shin, Mi-Sook,Choi, Sun-Mi 한방비만학회 2008 한방비만학회지 Vol.8 No.1

Objectives We have investigated a questionnaire on syndrome differentiation pertaining to obesity. To calculate data from this questionnaire, we can simply sum up the degree of symptoms. However, this does not reflect the difference in contribution of syndrome differentiation. In order to improve the level of precision of this questionnaire, we gather the weight of each symptom from experts and apply them to overweight persons. Method Nine Experts from The Society of Korean Medicine for Obesity Research nominated weights for the symptoms. We created a program based on weight survey results and applied to 1487 overweight persons and 26 oriental medical doctors. The concordance rate between the result obtained from the oriental medical doctors and that obtained using three methods was analyzed. Results 1. The reliability of this questionnaire is very high (Cronbach' ${\alpha}$=0.963). 2. The concordance level between diagnosis by oriental medical doctors and the result of general calculation is 0.347, between diagnosis by oriental medical doctors and the result of weighted calculation by syndrome differentiation is 0.362, between diagnosis by oriental medical doctors and the result of weighted calculation by symptoms is 0.1. Conclusions Weighted calculation by syndrome differentiation is relative more appropriate among three methods studied.

한방임상사용 의료기기의 실태 조사를 통한 개선방향 제시

문진석,김종열,류연희,송낙근,김혁주,이정림,이재원,최선미,Moon, Jin-Seok,Kim, Jong-Yeol,Ryu, Yeon-Hee,Song, Nak-Kun,Kim, Hyeog-Ju,Lee, Jeong-Rim,Lee, Jae-Won,Choi, Sun-Mi 한국한의학연구원 2005 한국한의학연구원논문집 Vol.11 No.1

We conduct a survey of actual condition of medical devices in korea traditional medicine on 157 oriental medicine doctors and 183 manufacturers. The 61% percent of doctors indicate the trouble in use of medical devices which is applied to korea traditional medicine. The manufacturers replied that devices have a problem with diagnosis(53%) and treatment(36%). To overcome these problems, it is necessary to develop devices based on the study of basic oriental medicine and the reliable clinical research. This study contributes the improvement of quality and the revision of rules on medical devices.

한방 의료기기 표준화 동향

문진석,최선미,김용석 대한전자공학회 2016 전자공학회지 Vol.43 No.12

한의사의 전침사용 임상실태 조사보고

문진석,이상훈,김정은,김보영,최선미 대한침구의학회 2011 대한침구의학회지 Vol.28 No.6

Objectives : This study was conducted to investigate status of electroacupuncture(EA) stimulation and adverse effects as a basic information of EA clinical treatment. Methods : The survey was carried twice by e-mail and online service against the traditional Korean medicine doctors registered in the Association of Korea Oriental Medicine. Results : Seventy-eight percent of 284 responders used the EA. The EA was the most widely used for the stimulation of the meridian-muscle on musculo-skeletal pain. In terms of methodological procedure, doctors mainly apply acupuncture with the needle of 2 or 3 pairs of 0.25mm diameter to the depth of 10-50mm for 15 minutes. Respondents used mainly at the frequency of 10Hz and the automatic setting at the intensity of around 3-4 grade. The adverse effects were investigated less then 3% in the 45.9% respondents and non in the 40.5%. The most priority content for better EA treatment was “development of the effective stimulation program”. Conclusion : In the result of survey, the frequency of practical use of EA was very high but the applied disease was concentrated in the pain. Hereafter, if the clinical study for safety and effectiveness is secured and more convenient EA tool is developed, the clinical application of the EA is likely to be more expanded.

Isolation and Characterization of Histamine-Producing Bacteria from Fermented Fish Products

문진석,김소영,조경주,양승준,윤건묵,엄현주,한남수 한국미생물학회 2013 The journal of microbiology Vol.51 No.6

Histamine is mainly produced by microorganisms that are found in fermented foods, and is frequently involved in food poisoning. Two histamine-producing bacteria were isolated from fermented fish products, anchovy sauce, and sand lance sauce by using a histidine decarboxylating medium. The species were identified as Bacillus licheniformis A7 and B. coagulans SL5. Multiplex PCR analysis showed the presence of the conserved histidine decarboxylase (hdc) gene in the chromosome of these bacteria. B. licheniformis A7 and B. coagulans SL5 produced the maximum amount of histamine (22.3±3.5 and 15.1±1.5 mg/L, respectively). As such, they were determined to be potential histamine-producing bacteria among the tested cultures.

Genome sequence analysis of potential probiotic strain Leuconostoc lactis EFEL005 isolated from kimchi

문진석,최혜선,신소연,노솔지,전체옥,한남수 한국미생물학회 2015 The journal of microbiology Vol.53 No.5

Leuconostoc lactis EFEL005 (KACC 91922) isolated from kimchi showed promising probiotic attributes; resistance against acid and bile salts, absence of transferable genes for antibiotic resistance, broad utilization of prebiotics, and no hemolytic activity. To expand our understanding of the species, we generated a draft genome sequence of the strain and analyzed its genomic features related to the aforementioned probiotic properties. Genome assembly resulted in 35 contigs, and the draft genome has 1,688,202 base pairs (bp) with a G+C content of 43.43%, containing 1,644 protein-coding genes and 50 RNA genes. The average nucleotide identity analysis showed high homology (≥ 96%) to the type strain L. lactis KCTC3528, but low homology (≤ 95%) to L. lactis KCTC3773 (formerly L. argentinum). Genomic analysis revealed the presence of various genes for sucrose metabolism (glucansucrases, invertases, sucrose phosphorylases, and mannitol dehydrogenase), acid tolerance (F1F0 ATPases, cation transport ATPase, branched-chain amino acid permease, and lysine decarboxylase), vancomycin response regulator, and antibacterial peptide (Lactacin F). No gene for production of biogenic amines (histamine and tyramine) was found. This report will facilitate the understanding of probiotic properties of this strain as a starter for fermented foods.