Xylazine의 진정효과와 α-adrenergic 수용체 봉쇄약물의 길항효과

김충희,하대식,김양미,김종수,Kim, Chung-hui,Hah, Dae-sik,Kim, Yang-mi,Kim, Jong-shu 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

The central nervous system depressant effect of xylazine and xylazine-ketamine was studied in chicken and mice. Intraperitoneal injection of xylazine(1~30 mg/kg) and xylazine(1~30 mg/kg)-ketamine(100 mg/kg) induced a loss of the righting reflex in chicken and mice, respectively. These effects of xylazine were dose-dependent. The results obtained were as follows; 1. The effect of xylazine-induced depression was antagonized by adrenergic antagonists having ${\alpha}_2$-blocking activity(yohimbine, tolazoline, piperoxan and phentolamine). 2. Yohimbine was most effective in the reduction of the CNS depression by xylazine. 3. Phenoxybenzamine and prazosin did not reduced CNS depression by xylazine in both species. 4. Labetalol (${\alpha}_1$, ${\beta}_1$-adrenergic antagonist) and propranolol(${\beta}$-adrenergic blocking agent) were not effective in reducing xylazine induced depression. 5. Cholinergic blocking agents (atropine and mecamylamine), a dopaminergic antagonist (Haloperidol), a histamine $H_1$-antagonist(chlorpheniramine), a histamine $H_2$-antagonist(cimetidine), a serotonergic-histamine $H_1$ antagonist(cyproheptadine) were not effective in reducing xylazine-induced depression. 6. Xylazine-induced depression is mediated by ${\alpha}_2$-adrenergic receptors and appears not to be involved in cholinergic, dopaminergic, serotonergic or histaminergic pathways.

DNA-PK-null 세포주의 adriamycin 처리에 의한 G₂/M 세포주기 변화

김충희,김종수,김나리,김의용,한진,Kim, Chung-Hee,Kim, Jong-Soo,Van Cuong, Dang,Kim, Na-Ri,Kim, Eui-Yong,Han, Jin 한국생명과학회 2003 생명과학회지 Vol.13 No.3

While the DNA-protein kinase (DNA-PK) complex, comprised of DNA-PKcs and Ku80, is primary involved in the repair of DNA double-strand breaks, it is also believed to participate in additional cellular processes. Here, treatment of embryo fibroblasts (MEFs) derived from either wild-type (Wt) or DNA-PKcs-null (DNA-$PKcs^{-/-}$) mice with various stress inducing agents revealed that adriamycin was markedly more cytotoxic for $Ku80^{-/-}MEFs$ and led to their long-term accumulation in the $G_2$/M phase. This differential response was not due to differences in DNA repair, since adrimycin-triggered DNA damage was repaired with comparable efficiency in both Wt and $Ku80^{-/-}MEFs$, but was associated with differences in the expression of important cell cycle regulatory genes. Our results support the notion that Ku80-mediated cytoprotection and $G_2$/M-progression are not only dependent on the cell's DNA repair but also may reflect Ku80's influence on additional cellular processes such as gene expression. DNA 손상 유발을 위해 cisplatin, mitomycin 그리고 adriamycin을 농도별로 처리하여 세포독성 효과 및 세포주기 분포를 조사하였다. 이들 약제중 adriamycin의 감수성이 가장 높았으며 특히 $Ku80^{-/-}MEFs$가 현저한 세포독성 감수성 효과를 나타내었다. DNA 회복과 관련된 S phase의 분포도를 알아보기 위하여 adriamycin을 처리한 결과 DNA-$PKcs^{-/-}MEFs$와 $Ku80^{-/-}MEFs$ 모두에서 S phase는 대조군과 비슷하게 나타났다. 그리고 DNA$PKcs^{-/-}MEFs$에 adriamycin 처리시 6시간 경과 후 $G_2$/M phase가 증가되었으나 30시간 경과시 정상으로 회복되었다. 그러나 $Ku80^{-/-}MEFs$는 6시간 경과 이후 36시간 경과시 까지 $G_2$/M phase가 지속적으로 증가하다 결국 사멸되었다. 따라서 Ku80는 세포주기 조절 유전자의 발현을 위해 필수적인 단백질이며 Ku80의 결핍은 $G_2$M phase에서 다음 단계로의 세포주기 변화를 상실하여 사멸하게 된다. 그러므로 $Ku80^{-/-}MEFs$가 대조군과 다른 반응을 나타내는 것은 DNA 회복정도의 차이에서 오는 것이 아니라 세포주기 조절유전자 발현의 차이에서 오는 것으로 사료된다.

시료고체상분산처리와 액체크로마토그라피를 이용한 소의 근육, 간 및 천엽에서의 벤지미다졸계 구충제 잔류분석

김충희,김곤섭,박정희,하대식,류재두,손성기,허정호,정명호,김종수,Kim, Chung-Hui,Kim, Gon-Sup,Park, Jung-Hee,Hah, Dae-Sik,Ryu, Jae-Doo,Son, Sung-Gi,Heo, Jung-Ho,Jung, Myung-Ho,Kim, Jong-Shu 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.2

Simultaneous multiresidue analysis using liquid chromatography determination for five benzimidazole anthelmintics(thiabendazole, oxibendazole, albendazole, mebendazole and fenbendazole) in bovine muscle, liver and omasum has been described. Blank or benzimidazole-fortified samples(0.5g) were blended with bulk $C_{18}$($40{\mu}m$, 18% load, endcapped, 2g). A column made from the resultant $C_{18}$/animal tissue matrix was first washed with hexane($8m{\ell}$), following which the benzimidazoles were eluted with acetonitrile($8m{\ell}$). Analytes of extracted sample were determined by liquid chromatography with UV detector at 290nm. Correlation coefficients of standard curves for individual benzimidazole isolated from fortified samples, using internal standardization, were linear($0.991{\pm}0.007$ to $0.996{\pm}0.005$) with average relative percentage recoveries from $62.1{\pm}3.8(%)$ to $92.3{\pm}7.5(%)$ for the concentration range($0.2{\sim}6.4{\mu}g/g$), respectively. Recoveries rates of TBZ, MBZ in liver, OBZ, MBZ in muscle and TBZ, MBZ in omasium from fortified benzimidazole were 92.%, 87.3%, 74.5%, 82.7%, 75.2% and 83.5% at condition II, respectively. Condition II showed higher recoveries rates than condition I. These results indicated that the matrix solid phase dispersion(MSPD) methodology is acceptable for the determination of 5 benzimidazole anthelmintics and may also suitable for other matrixes of food animal origin.

Epidermal Growth Factor가 난소 기형암종 배아세포주의 생존율에 미치는 효과

김충희,김종수,김나리,김의용,한진,Kim, Chung-hui,Kim, Jong-shu,Cuong, Dang Van,Kim, Na-ri,Kim, Eui-yong,Han, Jin 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.2

Human ovarian cancerous cells survive in a way that they trigger the nucleotide excision repair (NER) or double-strand DNA repair (dsDNA) repair mechanism to show resistance to anticancer drugs and activate many kinds of repair protein, thus removing damaged DNAs. Two experiments on the PA-1 human ovarian teratocareinoma cell line that hardly has any expression of epidermal growth factor receptor (EGFR) were conducted in the study; first, EGF-R was transfected and its receptor was obtained. The receptor was investigated in terms of its mutual relations with many kinds of protein concerning NER or dsDNA repair. Second, it was examined what kind impact cisplatin and adriamycin had on the effects of EGF-R over the PA-1 cell line lacking EGF-R. When being administered with cisplatin and adriamycin, Hey and Hey C2 cell lines showed a high level of resistance while PA-1 cell line a high level of sensitivity. Hey and Hey C2 cell lines that are resistant against anticancer drugs exhibited a high level of EGF-R expression while PA-1 cell line that is sensitive to them did a much lower level of the expression. When PA-1 cell line was transfected for the expression of DNA adduct and EGF-R, it showed a higher level of resistance compared to the control group. There was no difference in the expression of DNA repair proteins (DNA- dependent protein kinase, Ku70, and Ku80) between Hey and the PA-1 cell lines. The results indicate that the Hey cell line that is resistant against cisplatin and adriamycin works along the signaling system responding to the changes of EGF-R while the PA-1 cell line that is sensitive to both of them does to the lack of EGF-R.

체신에 관(關)한 문헌적(文獻的) 고찰(考察)

김충희,유동열,Kim Chung-Hui,Yu Dong-Yeol 대한한방소아과학회 1997 대한한방소아과학회지 Vol.11 No.1

2001년 농작물 병해 발생개황

김충희,Kim, Choong-Hoe 한국식물병리학회 2002 식물병연구 Vol.8 No.1

2001년 기상의 특징은 3월부터 5월까지 계속된 극심한 가뭄과 생육기간 내내 계속된 고온현상 그리고 별다른 태풍의 내습 없이 9, 10월 수확기 맑은날의 지속으로 요약할 수 있다. 벼는 순조로운 기상 때문에 잎, 이삭도열병, 세균성벼알마름병, 흰잎마름병, 잎집무늬마름병의 발생이 예년에 비해 경미하였으나, 감비추진 정책에 의해 생육후기 깨씨무늬병이 발생하여 전국적으로 문제시되었으며 그동안 발생이 적었던 줄무늬잎마름병이 경기, 충청, 호남 서해안지역을 중심으로 많이 발생하였다. 고추는 생육기 비가 많이 왔던 전남북 지역에서 역병 발생이 심하였으며 토마토와 오이는 대부분 예년에 비해 병발생이 적었다. 수박의 경우는 꽃자리 부분이 썩는 푸른곰팡이병이 전국적으로 발생하였으며 CGMMV는 전년에 비해 상당폭 발생면적이 감소하였고 노지재배의 경우 탄저병 발생이 예년에 비해 심하였다. 참외와 딸기도 전년에 비하여 병발생이 경미하였으며 예년에 발생이 많았던 참외의 급성위조증상도 발생이 대폭 감소하였다. 마늘·양파의 흑색썩음균핵병은 봄철의 가뭄과 고온현상으로 병발생의 전년의 절반수준으로 감소하였다. 감자의 풋마름병은 발병포장이 증가하는 경향이었으며 고구마의 덩굴쪼김병도 발생이 감소하였다. 사과의 경우 전반적으로 병 발생이 예년에 비해 경미하였으며 배는 흰가루병이 많이 발생하였다. 맥류의 붉은곰팡이병은 예년처럼 거기 발생이 없었다. Climate in the year of 2001 could be summarized as severe drought from March to May, unusually high temperature in whole plant growth periods and clear weather condition especially in harvesting time of September and October without any typoons. In rice, major diseases such as leaf and panicle blast, bacterial blight, sheath blight and bacterial grain rot developed little due to unfavorable weather conditions, however, brown spot occurred severely due partly to the reduced use of N-fertilizer, Rice stripe virus infection was unusually high mainly in the west coast areas with four times more diseaseD area campared to the previous year, In pepper phytophthora blight was extremely severe in Cheonnam and Cheonbuk provinces, where had frequent rainfalls during growing period. Incidence of major diseases of tomato and cucumber in 2001 was relatively mild. In watermelon, penicillium fruit rot, one of the peculiar disease, spread over major production areas, whereas CGMMV, usually severe in every years was much reduced. Watermelon plants growing in open-fields were more severely diseased than those in plastic houses. Major diseases of chinese melon and strawberry occurred slightly and in particular, sudden wilt syndrome of chinese melon which was severe in 2000 showed mild infections in 2001. Incidence of white rot of garlic and onion was much lower unlike other years due mainly to spring drought and high temperature in the growing period. Infected area of potato bacterial wilt tended to increase in 2001 while fusarium wilt of sweetpotato was decreased. Apple diseases were generally mild, but powdery mildew of pear increased sharply. Barley scab was not severe as seen in other years.

Epidermal Growth Factor가 난소 기형암종 배아세포주의 생존율에 미치는 효과

김충희(Chung Hui Kim),김종수(Jong Shu Kim),Dang Van Cuong,김나리(Na Ri Kim),김의용(Eui Yong Kim),한진(Jin Han) 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.2

가솔린 엔진용 타이밍 체인의 소음 특성에 관한 연구

김충희 ( Chunghui Kim ),한상욱 ( Sangwook Han ),김현철 ( Hyunchul Kim ),박영균 ( Youngkyun Park ),김영진 ( Youngjin Kim ) 한국액체미립화학회 2018 한국액체미립화학회 학술강연회 논문집 Vol.2018 No.-

As the number of revolutions of the sprocket increased, the overall noise of the system and the chain noise increased linearly. The maximum noise was overall noise 87.4dB(A) and chain noise 65dB(A) at 7,000rpm. Further research is needed on the resonance caused by the tension applied to the chain and the system structure in the future and the change of the noise characteristics accordingly.

냉각 갤러리 형상에 따른 디젤엔진용 피스톤의 온도 특성

김충희(Chunghui Kim),한상욱(Sangwook Han),이기수(Geesoo Lee),김현철(Hyunchul Kim),유연설(Younsul Yoo),김희수(Heesu Kim) 한국자동차공학회 2013 한국자동차공학회 학술대회 및 전시회 Vol.2013 No.11

승용 디젤엔진의 연소실 압력 변화에 따른 피스톤 온도 특성

김충희 ( Chung Hui Kim ),이기수 ( Gee Soo Lee ),김현철 ( Hyun Chul Kim ),한상욱 ( Sang Wook Han ),유연설 ( Youn Sul Yoo ),김희수 ( Hee Su Kim ) 한국액체미립화학회 2014 한국액체미립화학회 학술강연회 논문집 Vol.2014 No.-

In this study, the characteristics of piston temperature according to the combustion pressure were analyzed in a passenger car diesel engine. The peak combustion pressure was increased from 170bar to 210bar by adjusting the control parameters such as injection timing and intake pressure. In addition, effects of the shapes and locations of cooling gallery inside the re-melting piston on the piston temperature were evaluated. As the combustion pressure increased, the average piston temperature increased up to 21% in the base piston, while that in the piston with the re-melting technique and larger cooling gallery area increased up to 18 %. On the other hand, the piston with the larger cooling gallery area as well as raised cooling gallery to the piston crown side showed a similar temperature level with the base piston at the 170bar combustion pressure condition.