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김진규,김원록,이영근,김재성,송희섭,정철영,김기남,서원선,홍광표 ( Jin Kyu Kim,Won Rok Kim,Young Keun Lee,Jae Sung Kim,Hi Sup Song,Cheorl Young Jeong,Ki Nam Kim,One Sun Suh,Kwang Phyo Hong ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2
Tradescantia in BNL 4430 is a hybrid clone heterozygous for its flower color and extremely sensitive to ionizing radiation. Such a fact made people around nuclear power plants be interested in Tradescantia, a botanical tester of radiation effects. The pur pose of this study is to provide the botanical supplement to physical radiation monitor systems and also to ascertain biological safety regarding environmental radiation around nuclear power plants. Flowers collected periodically from three Tradescantia gardens (in Youngkwang NPP Jeonsigwan. City office and KAERI. respectively) were scored for pink mutation frequencies in stamen hair cells. Pink mutation frequencies in the study area were in the range of 0.82∼1.14. which were slightly lower than the annual mean background mutation frequency of 1.16. Statistical analysis of scoring data showed that there was no significant difference between three Tradescantia gardens (p>0.05). From the result, the environmental radiation level around Youngkwang nuclear power plant can be said biologically safe. Further study for verifying safety will make a role for improving public acceptance related to the peaceful use of nuclear power.
중성자와 감마선 중복 조사가 자주달재비 분홍돌연변이율에 미치는 영향
김진규,이영근,김재성,신해식,현성희,Kim, Jin-Kyu,Lee, Young-Keun,Kim, Jae-Sung,Shin, Hae-Shick,Hyun, Soung-Hee 대한방사선방어학회 2000 방사선방어학회지 Vol.25 No.2
The effects of combined irradiation of neutrons and ${\gamma}$-rays were analysed by means of Tradescantia stamen hair (TSH) assay. Potted plants were irradiated with $0{\sim}2$ Gy of ${\gamma}$-rays. For the combined treatments, the plants were irradiated with neutrons from $^{252}Cf$ 24 hours before or after ${\gamma}$-irradiation. The slopes of dose-response curves were 5.98, 6.17 and 7.48, in ${\gamma}$-rays, ${\gamma}$-rays+neutrons, and neutrons+${\gamma}$ -rays irradiated groups, respectively. The biological efficacy of radiations in the induction of pink mutations increased by 25% in neutrons+${\gamma}$-rays irradiated group, while it increased by 12% in ${\gamma}$-rays+neutrons irradiated group. The combined irradiation with two kinds of radiations results in the different efficacy of radiation depending on the order of irradiations, which is of great importance in the related fields such as sterilization and radiotherapy. 자주달개비 식물체에 감마선과 중성자를 중복 조사하였을 패 나타나는 수술털(TSH) 분흥돌연변이율의 변화를 분석하였다. 감마선은 $0{\sim}2$ Gy의 범위에서 선량별 조사를 하였으며 중성자는 $^{252}Cf$ 선원으로부터 일정한 거리에 식물체를 배열하여 조사하였다. 감마선을 선량별로 조사한 후 중성자를 조사한 때의 TSH 분홍돌연변이 감마선량-반응의 증가계수는 6.17로 감마선량-반응식의 5.98에 비해 12%의 방사선효율 증가를 초래했으며 중성자를 먼저 조사한 다음 감마선을 조사한 경우는 증가계수가 7.48로서 25%의 방사선 생물효율 증대가 나타났다. 두 종류의 방사선 중복 조사시 조사방식의 선 후 조합에 따른 방사선생물효율 증대가능성은 멸균 또는 세포사멸 등 방사선을 긍정적으로 이용하는 분야에 있어 매우 중요한 의미가 있다.
확장 해밀턴 이론에 근거한 탄점소성 시스템의 시간유한요소해석법
김진규,Kim, Jin-Kyu 한국공간구조학회 2014 한국공간구조학회지 Vol.14 No.1
In order to overcome the key shortcoming of Hamilton's principle, recently, the extended framework of Hamilton's principle was developed. To investigate its potential in further applications especially for material non-linearity problems, the focus is initially on a classical single-degree-of-freedom elasto-viscoplastic model. More specifically, the extended framework is applied to the single-degree-of-freedom elasto-viscoplastic model, and a corresponding weak form is numerically implemented through a temporal finite element approach. The method provides a non-iterative algorithm along with unconditional stability with respect to the time step, while yielding whole information to investigate the further dynamics of the considered system.
자주달개비 화분모세포 미세핵 생성률의 방사선량-반응 관계
김진규,송희섭,현성희,Kim, Jin-Kyu,Song, Hi-Sup,Hyun, Soung-Hee 대한방사선방어학회 1999 방사선방어학회지 Vol.24 No.4
본 연구는 이온화 방사선이 자주달개비 화분모세포의 미세핵 생성에 미치는 영향을 분석하여 선량-반응 관계를 수립하고자 수행되었다. 자주달개비의 화분모세포는 감수분열을 통하여 4개의 꽃가루로 발전되는 생식세포로서 분열과정 중에 방사선에 노출되면 염색체 조각이 분리되어 미세핵을 형성한다. 감마선 선량이 증가함에 따라 미세핵 생성률도 증가하였으며 특히 50 cGy까지의 선량범위에서 선량별 미세핵 생성률의 최대값은 뚜렷한 선량-반응관계를 보였다. 선량-반응 관계식을 통해 볼 때 1 cGy의 부가적 선량은 100 사분자당 최대 2개씩의 미세핵을 추가로 생성시키는 것으로 나타났다. 화분모세포 미세핵 생성률의 방사선 지표성은 생물학적 방사선량 감시를 위한 필수선결사항일 뿐 아니라 공해물질의 생체 위해성 검증, 수질과 토양의 건전성 평가 등에 대한 응용성을 갖고 있다. This study was carried out to investigate the radiation dose-response of micronucleus frequencies in Tradescantia pollen mother cells. The number of micronuclei increased in the tetrads as a result of chromosome deletion after irradiation. The maximal frequency of micronuclei showed a good dose-response relationship in the range of dose $0{\sim}50$ cGy. On the basis of the relationship, a dose of 1 cGy results maximally in two additional micronuclei in 100 tetrads. The radiation dose-response relationship of micronucleus occurrence is prerequisite to biological monitoring of radiations. The micronucleus assay can be applied to biological risk assessment of environmental toxicants, and to integrity test of water or soils of interest.
Artemia ( Class Crustacea ) 알의 부화에 미치는 이온화방사선의 효과
김진규,김원록,이영근,이창주,장화형 ( Jin Kyu Kim,Won Rok Kim,Young Keun Lee,Chang Joo Lee,Hwa Hyoung Chang ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2
Tradescantia in BNL 4430 is a hybrid clone heterozygous for its flower color and extremely sensitive to ionizing radiation. Such a fact made people around nuclear power plants be interested in Tradescantia, a botanical tester of radiation effects. The pur pose of this study is to provide the botanical supplement to physical radiation monitor systems and also to ascertain biological safety regarding environmental radiation around nuclear power plants. Flowers collected periodically from three Tradescantia gardens (in Youngkwang NPP Jeonsigwan. City office and KAERI. respectively) were scored for pink mutation frequencies in stamen hair cells. Pink mutation frequencies in the study area were in the range of 0.82∼1.14. which were slightly lower than the annual mean background mutation frequency of 1.16. Statistical analysis of scoring data showed that there was no significant difference between three Tradescantia gardens (p>0.05). From the result, the environmental radiation level around Youngkwang nuclear power plant can be said biologically safe. Further study for verifying safety will make a role for improving public acceptance related to the peaceful use of nuclear power.
지하철 시설내 부유먼지에 함유된 돌연변이원의 생물학적 영향 평가
김진규(Jin Kyu Kim 외 4명),신해식,이정주,김 균,이진흥 환경독성보건학회 2002 환경독성보건학회지 Vol.17 No.3
Airborne pollutants in the subway facilities can be potentially harmful to the health of passengers. This study was designed to examine whether the suspended particulates have mutagenic or carcinogenic effect on the plant cell systems. Total suspended particulates were collected with a high volume air sampler, in the entrance, the waiting room, and the platform of each subway station. The biological end-points in this experiment were the pink mutations in stamen hairs and micronuclei in the pollen mother cells of Tradescantia. The exudates were collected by shaking the filter papers from the sampler in distilled water for 24 hours. All the plant cuttings exposed to the exudates resulted in positive responses. The micronucleus assay proved more reliable and sensitive to the test than the stamen hair assay. The results indicate that the air particulates can give an adverse effect on the health of subway passengers.
자주달개비 수술털에서 방사선에 의해 유발되는 분홍돌연변이에 대한 광주기의 영향
김진규(Jin Kyu Kim),김원록(Won Rok Kim),(A . Cebulska Wasilewska) 한국환경생물학회 1999 환경생물 : 환경생물학회지 Vol.17 No.3
The present study was carried out to investigate the combined effect of radiation and photoperiod (PP) regimes on Tradescantia 4430 somatic cell mutations. Potted plants were irradiated with 0.3, 0.5 and 1.0 Gy of gamma radiation from ^60Co source. The plants irradiated only with gamma radiation were used as control group (CT). The somatic cell mutation rate in 0.5 Gy irradiated CT and PP20 group started to increase on the 6th day and reached a maximum value on the 10th day and 9th day after irradiation while the rate in the experimental group under 4 hours of photoperiod a day (PP4) started to increase on the 10th day and reached a maximal value on the 16th day post-irradiation. The slope of dose-response curve in CT was 5.99 (r^2=0.99), while it was 6.93 (r^2=0.98) in PP20 and 11.74 (r^2=0.99) in PP4, respectively. The biological efficacy of radiation in the induction of pink mutation increased by 15.7% in PP20 and 95.9% in PP4, respectively. It is suggested that photoperiod regimes unfavorable to the plant have an additive effect on radiation-induced mutations and a delaying or inhibiting effect on cell damage repair, as well. [photoperiod, pink mutation, radiation, Tradescantia].
자주달개비 수술털에서 방사선에 의해 유발되는 세포손상에 대한 살충제의 방어효과
김진규(Jin Kyu Kim),김원록(Won Rok Kim),이영근(Young Keun Lee),장화형(Hwa Hyoung Chang),이창주(Chang Joo Lee) 한국환경생물학회 1999 환경생물 : 환경생물학회지 Vol.17 No.1
To investigate the combined effect of radiation and pesticide on Tradescantia somatic cell mutations, potted plants of Tradescantia 4430 on which parathion had been sprayed evenly 24 hours before irradiation. Radiation doses were 0.3, 0.5, 1.0 and 2.0 ㏉ of gamma-ray. The plants irradiated only with the gamma-ray radiation were used as control groups(CT). Pink mutation frequency increased linearly proportional to the radiation dose and the peak interval of elevated mutation frequencies appeared during 7∼11 days after irradiation in both CT and Pa+γ groups. The slope of dose-response curve in CT was 5.99(r^2=0.988), while it was 3.43(r^2=0.981) in Pa+γ. It seemed that parathion pretreatment had a protective effect against radiation-induced cell damages since it decreased the slope value by 43%. It is suggested that an adaptive response or radiomodification could be induced in irradiated stamen hair cells by parathion pretreatment.