국내 경북지역 소에서 분리된 브루셀라 분리주의 생물학적 특성

김정화,임정주,김동혁,이진주,김대근,전무형,김상훈,장홍희,이후장,민원기,김석,Kim, Jeong-Hwa,Lim, Jeong Ju,Kim, Dong Hyeok,Lee, Jin Ju,Kim, Dae Geun,Jun, Moo-Hyung,Kim, Sang Hun,Chang, Hong Hee,Lee, Hu Jang,Min, Won-Gi,Kim, Suk 대한수의학회 2010 대한수의학회지 Vol.50 No.2

Members of the genus Brucella are facultative intracellular bacteria and cause brucellosis, a chronic disease in humans and abortion in animals. In this study, we tested sera for brucellosis of 15 Hanwoo farms in the western part of Gyeong-buk province, resulting 5 farms were brucellosis positive in 2008. We collected blood from 277 heads in the brucellosis positive 5 farms, and performed serological diagnosis, brucella positive cattle which had shown higher than 200 antibody titer in tube agglutination test were slaughtered, supramammary lymph nodes were collected, and Brucella spp. wild type isolation and identification were performed. From these results, 15 of Brucella spp. wild type strains were isolated and all strains were identified as B. abortus biotype 1 by biological and molecular analysis. In the antimicrobial susceptibility test, all 15 strains had a similar susceptibility and resistance pattern. This study may be useful for bacteriological and epidemiological understanding of cattle brucellosis in Korea.

벼멸구의 유기인계(有機燐系) 살충제저항성(殺蟲劑抵抗性)에 대(對)하여(II) 생화학적특성(生化學的特性) 비교(比較)

김정화,황태구,Kim, Jeong-Wha,Hwang, Tae-Gu 한국응용곤충학회 1987 한국식물보호학회지 Vol.26 No.3

본(本) 실험(實驗)은 벼멸구에 대한 반수치사약량(半數致死藥量)의 차이(差異)를 나타내는 저항성(抵抗性) 및 감수성계통(感受性系統)과 그들의 교잡종(交雜種) $F_1$에 대(對)한 생화학적(生化學的) 특성(特性)을 구명(究明)하고자 실시(實施)하였다. 살충제무처리(殺蟲劑無處理)의 esterase활성(活性)은 저항성계통(抵抗性系統)과 교잡종(交雜種) $F_1$이 감수성계통(感受性系統)에 비(比)하여 높았으며, diazinon, MEP, BPMC 처리후(處理後) esterase의 활성변화(活性變化)는 저항성계통(抵抗性系統)과 교잡종(交雜種) $F_1$에서는 별차이(別差異)가 없었으나 감수성계통(感受性系統)에서는 현저(顯著)히 떨어졌다. Esterase의 높은 활성(活性)은 저항성발달(抵抗性發達)과 관계(關係)가 있었으며 교잡종(交雜種) $F_1$에서 esterase 활성(活性)이 높게 나타난 것은 저항성계통(抵抗性系統)이 우성인자(優性因子)로 유전(遺傳)됨을 알 수 있었다. This experiment was carried out to evaluate the difference in the biochemical characteristic of the brown planthoppers of the insecticide risistant, susceptible strains and their hybrid progenies. Activity of the esterase isozyme separated by electrophoresis method was remarkably high in the resistant strain as compared with the susceptible strain. Esterase activity between the insecticide-treated strains and the non-insecticide strains was not degraded in the resistant strain and the $F_1$, but remarkably degraded in the susceptible strain. The increase of esterase activity was associated with the development of resistance, and that was inherited with a dominant gene.

새로운 직파재배기술 "무논골점파"

김정화,Kim, Jeong-Hwa 전국농업기술자협회 2008 농업기술회보 Vol.45 No.3

무논골점파 재배기술은 초기생육이 우수하고, 잡초성 벼 발생을 방지할 수 있으며, 특히 기계이앙과 비교하여 수량과 품질의 차이가 없는 새로운 직파재배기술이다.

기수(氣嗽)에 대(對)한 문헌적(文獻的) 고찰(考察)

김정화,Kim, Jung-Hwa 대한한방내과학회 1992 대한한방내과학회지 Vol.13 No.1

This study has been carried out to investigate the cause, symptom and treatment of Gi-Su(氣嗽) by referring to 20 literatures. The results were as follows; 1. The factors causing Gi-Su(氣嗽) divided into 3 groups. The 1st outer factors are mental element, 2nd spleen week 3rd dam(痰). 2. The symptom of Gi-Su(氣嗽) is as follows. stimulus feeling in throat, cough, pain of sub-ribs, bad feeling, retching, vomiting, unfair, fatigue, worn, diarrhea, dark tongue, makhyun(脈弦). 3. The treatment of Gi-Su(氣嗽) is as follows. gaewulhanggi(關鬱行氣), egichungpae(利膈淸肺), hwadam(化痰), soganhaeWul(疏肝解鬱), hanggidoche(行氣導滯), sangyuljedam(散結除痰) 4. The drugs of Gi-Su(氣嗽) is as follows. chilgitang(七氣湯), gamisachiltang(加味四七湯), sojaganggitang(蘇子降氣湯), banhahubaktang(半夏厚朴湯).

여말선초 약초원의 형성 과정과 조경사적 의미 고찰

김정화,Kim, Jung-Hwa 한국조경학회 2017 한국조경학회지 Vol.45 No.5

This study traces the development of physic gardens in Korea and explores their significance in the history of landscape architecture. For this purpose, records related to physic gardens from medical sources from the period of the Three States to the Joseon dynasty, when herbal medicine was systematized as a field, were searched. Physic gardens had been developed by the time of the late Goryeo and early Joseon dynasties, in the 13th and the 15th centuries. Yakpo(kitchen gardens for medicinal herbs) were cultivated by a group of new high-level officials in the late Goryeo dynasty, when an increasing interest in hyangyak(native herbs) emerged under the influence of the Neo-Confucian perspective on nature, which emphasized locality. The sources analyzed in this study confirm that physic gardens called jong-yakjeon(royal medicinal herb gardens) were in operation in the early Joseon dynasty when policies to investigate, discover, cultivate, and research native herbs were put into place. It is likely that the jong-yakjeon were established at the beginning of the Joseon dynasty as subsidiary facilities under its central medical institutions, the Naeuiwon and Hyeminseo, and then declined in the late Joseon dynasty. Jong-yakjeon can be confirmed to have existed in the mid-15th century. Physic gardens were located in several places outside the Fortress Wall of Hanyang, such as Yakhyeon, Yuldo, Yeoudo, and Saari. The total area encompassed by physic gardens was about 160,000 square meters in the early 18th century. In jong-yakjeon, dozens of medicinal herbs were cultivated, including Schizonepeta tenuifolia var. japonica, Rehmannia glutinosa, and Glycyrrhiza uralensis Fischer, and these gardens were operated by physicians dispatched from the Naeuiwon and dozens of provincial slaves. In conclusion, the jong-yakjeon were similar to the physic gardens of Renaissance medical universities in that they reflected the interest in and development of theories about new herbs, and were similar to the physic gardens of medieval castles and monasteries in terms of species types, location, and function. This paper has limitations in that it does not present the specific spatial forms of the yakpo or the jong-yakjeon. Nevertheless, this paper is significant for the field of garden history because it shows that physic gardens in Korea appeared in the late Goryeo and early Joseon dynasties concomitantly with the development of medicine towards native herbs and functioned as utilitarian gardens to cultivate community remedies.

방사선 조사가 백서 하악과두 연골 형성에 미치는 영향에 관한 형태계측학적 연구

김정화,허민석,이삼선,최순철,Kim Jeong-Hwa,Heo Min-Suk,Lee Sam-Sun,Choi Soon-Chul 대한영상치의학회 1999 Imaging Science in Dentistry Vol.29 No.1

Purpose: This study was undertaken to quantitatively estimate the degree of the damage and recovery of the irradiated rat condylar cartilage using the Image Analyzer. Materials and Methods: Experimental animals were 16 male rats of the Sprague-Dawley strain at the age of 20 day irradiated with the dose of 10 Gy in their head and neck region. Four rats were sacrificed at the each of the following time intervals - 1, 4, 7 and 14 days, respectively. The same number of control group animals were sacrificed at the each age of 21. 24, 27 and 34 days, respectively. The specimens were stained with 0.5% toluidine blue and examined with light microscope. The condylar cartilage was divided into 4 zones; fibrous zone, proliferating zone, upper hypertrophic zone, and lower hypertrophic zone. And then, the proliferating zone was subdivided into 2 layers - upper and lower layer, and upper and lower hypertrophic zone were subdivided into three layers, respectively - upper, middle and lower layer. With the aid of Image Analyzer, morphometric analysis was performed. The thickness, the numerical density of cells, the cell area density, the extracellular matrix area density, the mean area of single cell, the mean area of extracellular matrix per single cell were measured and analysed. Results: In the experimental group, the thickness of the fibrous zone was slightly increased and that of the proliferating zone and the upper and the lower hypertrophic zone was markedly decreased. With time, the thickness of the fibrous zone was gradually increased and that of the proliferating zone and the upper and the lower hypertrophic zone was steadily in the decreased state. The numerical density of cells of the proliferating zone was increased on post-irradiated 1 day, but decreased after post-irradiated 4 day, and that of the upper hypertrophic zone was decreased. The numerical density of cells of the lower hypertrophic zone was decreased in the early stage and then was decreased or not significantly different from that of the control group with time. In the experimental group, the cell area density of the fibrous zone and the proliferating zone was decreased in the early stage and then gradually increased or not significantly different from that of the control group with time. The cell area density of the upper and the lower hypertrophic zone was varied with time. The extracellular matrix area density value were totally opposite to the cell area density values: The mean area of single cell of the fibrous zone and the proliferating zone was .decreased on post-irradiated 1 day, and increased after post-irradiated 4 day. The mean area of single cell of the upper hypertrophic zone was varied with each layer and time. In the experimental group, the mean area of extracellular matrix per single cell of the fibrous zone was not significantly different with control group, and that of the proliferating zone was decreased on post-irradiated 1 day, and increased after post-irradiated 4 day. The mean area of extracellular matrix per single cell of the lower hypertrophic zone was increased in the early stage. and that of upper hypertrophic zone was varied with each layer and time. Conclusion: The condylar cartilages of rats were affected by irradiation, but the changes were vaned with each layer and time. By morphometric analysis. the changes of the cells of the condylar cartilage of irradiated rat could be calculated quantitatively.

일제강점기 대중잡지 『삼천리』를 통해 본 검열의 흐름과 매체의 생존 전략

김정화 ( Kim Junghwa ),문한별 ( Moon Hanbyoul ) 한국어문학국제학술포럼 2021 Journal of Korean Culture Vol.52 No.-

『Joseon Chulpan Gyeongchal Wolbo』 was a classified censorship document of the Japanese Government General’s Book Department formulated every month from September 1928 to December 1938. This report documents the total number of publications printed every month, administrative disposition statistics, and record of administrative disposition due to censorship, among other information. This article seeks to unfold the discussion regarding changes in the publication policy under the Empire and the media’s response strategy by utilizing the “article point” that documents the details of administrative disposition following censorship. In particular, it seeks to illuminate why the longest-running popular magazine during the period of Japanese colonialism, 『Samcheonli』’s, changed its publication direction, and to uncover how the proJapanese theory and sentiment formed and disseminated. The article point of 『Samcheonli』 has been classified, on the basis of its yearly proportion, into the three periods: 1929-1931, 1932-1936, and 1937-1938. The classification was made to examine the detailed aspect of censorship and administrative disposition. From 1929 to 1931, when 20 out of 29 article points were concentrated, articles with a nationalistic and socialist overtone were subject to censorship. From 1932 to 1936, the number of administrative dispositions decreased significantly compared to the previous period, and literary work appeared in the article points instead of other general articles or articles with a strong editorial characteristic. From 1937 to 1938, when the number of administrative dispositions increased rapidly, those articles with content contrary to the Japanese-Chosun identity policy (Naesun-Ilchae policy), those with favorable statements about the perspective of the U.S, which was regarded an enemy country in relation to the war, and those that were determined to contain contexts relating to nationalism and antiwar became subject to administrative disposition. The fact that 『Samcheonli』 was organized as article point for 29 occasions forms the basis of the Japanese Government General’s determination that this magazine contained ideologically problematic issues. Based on this, the historical meaning and the media’s response strategy can be confirmed through the characteristics of articles published for each period. From the point of the dissolution of the Shinganhyoe (an antiJapanese colonialism group), 『Samcheonli』’s characteristic transformed drastically from being suggestive and political to mass appeal-oriented, and its direction turned to securing readership and being plainly proJapanese. Following the emasculation of its political and ideological tendency of the early days, Donghwan Kim’s original intention of pursuing a popular general magazine emerged naturally, and as a result, 『Samcheonli』 settled based on the conspicuous capitalistic “sensationalism” and survival-based “proJapanese tendency.”

한국형 모빌리티 매니지먼트의 교통수단전환 영향분석

김정화,김숙희,류인곤,최기주,Kim, Jeong-Hwa,Kim, Suk-Hui,Ryu, In-Gon,Choe, Gi-Ju 대한교통학회 2015 교통 기술과 정책 Vol.12 No.3

의료개혁의 현안과 정책과제

김정화,이경원 ( Joung Hwa Kim,Kyung Won Lee ) 한국행정학회 1997 한국행정학보 Vol.31 No.4

지금의 우리나라 보건의료의 체계와 수준은 총체적 위기로 진단 내릴 수 있다. 이러한 위기는 보건의료가 내포하는 이념적, 경험적 측면 모두를 포괄하여 나타나고 있다. 먼저 과연 우리는 보건의료에 대한 분명한 합의된 이념적 입장이 있는지를 자문하고자 한다. 의료는 기본적으로 의료체계의 세 주체라 할 수 있는 의료공급자와 의료수요자인 국민 그리고 정부에 의해 이루어진다. 그러나 모두가 현재의 보건의료 체제에 만족하지 못한다는데 문제가 있다. 의료사고 내지 의료체계상의 문제가 제기됐을때 그에따른 정부의 미봉책으로 일관되어 온 것이 우리 보건의료정책의 현실이라 할 수 있다. 정부는 최근 건강권의 확보차원에서 의료제도의 기본틀을 재정립하고자 의료개혁위원회를 설치하였다. 이는 양질의 보건의료 서비스에 대한 국민적 욕구의 반영이라 할 수 있으며 의료시장의 개방 등 21세기의 환경변화에 능동적으로 대처하기 위한 정부의 정책적 접근이라 할 수 있다. 본 연구는 보건의료와 관련된 중심 이슈를 논의하고 가능한 정부의 역할을 살펴보는데 그 목적이 있다. 이러한 목적을 국민의 건강권에 관한 논쟁, 보건의료의 환경과 개혁과제 그리고 보건의료 분야에 있어 정부의 역할에 대해 살펴봄으로써 파악하고자 한다. 본 논문은 최근 진행되고 있는 의료개혁과 보건관련 정책수립에 방향을 제시하고자 한다.

고온 다습한 환경에서의 주관적 착용 쾌적감 평가도구 개발을 위한 기초 연구 - Fuzzy 이론의 적용방법과 요인분석 방법간의 비교 -

김정화,조승식 ( Jeong Wha Kim,Seung Sik Cho ) 한국의류학회 1996 한국의류학회지 Vol.20 No.2

Recently, need for the development of the quantification of subjective evaluation is growing for the production of high-touch and high-tech textile products. In this study, Fuzzy theory is utilized for the evaluation of the wear comfort of the various blouses. Result of a new evaluation method and factor scores, validity of the new evalution technique adopted fuzzy theory was crosschecked with the results of fator analysis and factor scores. As results, fuzzy theory was proved to be adequate methodology to objectify the subjective evaluation of the adequacy of clothing which is worn. When DUNCAN`S multiple comparion among median of the fuzzy composite score were compared with the results of factor score, the sensitivity of the test methods tends to increase. Therefore, it is suggested that fuzzy weighted checklist is an alternative evaluation scale for the subjective comparison of the textile products. In addition, individual median of fuzzy composite score value should be treated by statistical for the sensitive analysis of subjective evalution.