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      • KCI등재

        식중독 원인 미생물 장기 보존 방법에 대한 고찰

        김유진,김수민,김소연,윤요한,Kim, Yujin,Kim, Soomin,Kim, Soyeon,Yoon, Yohan 한국식품위생안전성학회 2022 한국식품위생안전성학회지 Vol.37 No.3

        The rapid development of biotechnology has increased the importance of microorganisms or their genetic information. Thus, the Nagoya Protocol on access to genetic resources and the fair and equitable sharing of benefits arising from their utilization was established, and countries are working to secure industrially and academically useful bioresources to deal with the agreement. In the case of Korea, because 67% of bioresources are imported from abroad, we are required to secure domestic bioresources as well. The number of isolated foodborne illness-causing microorganisms is predicted to invrease based on the incresing number of outbreaks of foodborne illness each year. Consequently, appropriate long-term preservation methods are necessary to secure the isolated microorganisms for the purpose of research and resourcification. Therefore, the long-term preservation methods for bacteria, fungi, viruses, and protozoa were investigated in this study, from domestic and international bioresource banks, and the functions of the cryoprotectants were reviewed and discussed. This review should be informative in the preservation of microorganisms and contribute to the development of biotechnology.

      • KCI우수등재

        미국 브라운필드 관리 프로그램과 정책의 시기별 특성과 함의

        김유진,패트릭 밀러,Kim, Eujin Julia,Miller, Patrick Korean Institute of Landscape Architecture 2015 韓國造景學會誌 Vol.43 No.1

        하수처리시설, 군부대 등의 산업이전적지의 공원화 계획이 늘어나면서 브라운필드 재개발은 조경계획 및 디자인에서 하나의 분야로 자리 잡았다. 특히, 주변 커뮤니티 활성화, 지가 상승 등 동반이익에 대한 기대가 높아지면서 선별된 부지들의 종합적 계획 및 관리에 대한 필요성이 인식되고 있다. 본 연구는 미국 브라운 필드 관리 정책의 변화양상을 살펴 본 기초적인 사례연구로서, 1980년 수퍼펀드 프로그램 도입 이후, 약 30 여 년간 확장하여 온 브라운필드 프로그램과 정책의 시기별 특성을 파악하는 것을 주 목적으로 한다. 연구 방법으로 서술적, 해석적 방법에 기반한 미국 환경부 산하 기관들의 웹문서 및 관련 문헌 분석을 통해 정책의 특성을 시계열적으로 분석하였다. 본 연구의 결과, 주요한 변화를 기점으로 하여, 환경규제시기, 오염정화 및 재개발 촉진시기, 종합적 계획시기의 세 가지 시기로 구분되었고, 주요 특성은 규제 완화 및 재조정, 지원 프로그램의 다양화, 하향식과 상향식 방식의 조화, 데이터베이스 구축의 네 가지 측면으로 요약되었다. 본 연구는 대상지 선별기준, 이해 당사자 파악 및 책임분쟁 조정, 오염도 평가비용 및 처리 등 브라운필드만이 가진 공통 문제들이 어떻게 다뤄져 왔는지 선례를 살펴봄으로써, 향후 한국의 체계적 브라운 필드 관리 및 계획에 시사점을 제공하는 것에 의의를 두고 있다. Brownfield sites are beginning to be considered as potentially useful areas for landscape design and planning, with post-industrial areas such as water treatment facilities and military training bases being converted into useful landscapes such as parks and recreation areas. These redevelopments bring broad benefits through revitalizing communities and increasing property values, thus, increasing the demand for comprehensive management and planning policies. This study examines changes in U.S. brownfield policies and programs and, identifies their periodic characteristics over the thirty years since the Superfund program was introduced in 1980. A descriptive and interpretive approach was utilized, focusing specifically on a time sequential analysis of the data gathered from the overview of the Environmental Protection Agency's web-based documents and related literature. The primary changes in and characteristics of programs and policies were analyzed and divided into three periods : environmental protection, remediation and reuse, and comprehensive planning. Four major features were identified: relaxation and readjustment of regulation, diversification of support programs, a mix of top-down and bottom-up approaches, and database system building. The study examines how common brownfield problems such as site identification difficulties and assessment and remediation cost have been dealt with in the regulatory context and has implications for future policies and programs for effective brownfield planning and management in Korea.

      • KCI등재

        항-보호항원 항체의 역가 측정을 위한 효소면역측정법 밸리데이션

        김유진,Kim, Yu-Gene 한국군사과학기술학회 2010 한국군사과학기술학회지 Vol.13 No.3

        The vaccine is biological pretreatment that improves immunity to a particular disease. We can get immunity from producing antibody with injection antigen which has ability to defense against the disease. The ELISA is the most widely used method to measure antibody titer. We have developed and performed validation of ELISA according to the guideline of KFDA and ICH. In this paper, we have verified ELISA method is an excellent method to measure the titer of anti-PA antibody. We have constructed recombinant protective antigen among anthrax toxins and used as antigen of ELISA. In this validation, we have evaluated precision (repeatability, interlaboratory precision), specificity, linearity(range) and LOD, which are validation articles suggested by guideline. Inter-person precision was replaced with inter-laboratory precision. From the results, we have confirmed high precision in all experiments with CV under 20%.

      • KCI등재

        정상 족과 무지 외반증에서의 무지 지간 외반증의 발생 빈도와 무지 외반각과 제1-2 중족골간 각과의 연관성에 대한 분석

        김유진,정홍근,방승철,Kim, You-Jin,Jung, Hong-Geun,Bahng, Seung-Chul 대한족부족관절학회 2007 대한족부족관절학회지 Vol.11 No.1

        Purpose: In order to achieve successful outcome for the hallux valgus surgery, it is mandatory to consider the possible associated hallux valgus interphalangeus, and therefore the hallux interphalangeal angle (HIA) other than hallux valgus angle (HVA) and intermetatarsal angle (IMA) has been well appreciated. The purpose of this study is to evaluate the incidence of hallux interphalangeal angle in the normal and hallux valgus feet and also the statistical correlations of HIA with HVA and IMA in the 2 groups. Materials and Methods: The study is base on the standing foot AP radiographs of the 100 normal feet (HVA<$12^{\circ}$ and $IMA<9^{\circ}$) and 100 hallux valgus feet (HVA>$25^{\circ}$ and IMA>$12^{\circ}$). We measured the hallux valgus angle, intermetatarsal angle and hallux interphalangeal angle, where we defined the hallux valgus interphalangeus when the HIA was more than $10^{\circ}$. We evaluated the incidence of the hallux valgus interphalangeus in the normal and hallux valgus groups and the correlations of the HIA with HVA and IMA. Results: In normal feet group, hallux valgus interphalangeus comprised 82%, while there were only 20% of HVI in hallux valgus group. Among 200 total feet, there was negative correlations between the HVA and HIA as well as IMA and HIA statistically (p<0.01). Conclusion: There was lower incidence of hallux valgus interphalangeus in the hallux valgus group compared to the normal feet group.

      • KCI등재

        Pin-site scar revision with subcisions and acellular dermal matrix

        김유진,Ji Hun Kim,김윤상,전영우 대한미용성형외과학회 2020 Archives of Aesthetic Plastic Surgery Vol.26 No.1

        External fixation is widely used in orthopedic and reconstructive surgery. However, it may leave retracted and depressed scars because it involves the percutaneous placement of transosseous pins or wires that are secured to an external scaffolding. These scars do not resolve or improve spontaneously, causing patients to experience dissatisfaction and possibly requiring a surgical intervention. Moreover, the management of scars that arise from external fixator pin sites is challenging because of their fibrotic and depressive features. However, by using subcisions and inserting acellular dermal matrix under the scars, the contour of the scars can be improved easily. Therefore, we present a case of pin-site scar revision using a technique that we developed.

      • KCI우수등재

        Collaborative Planning Model for Brownfield Regeneration

        김유진,패트릭 밀러,Kim, Eujin Julia,Miller, Patrick Korean Institute of Landscape Architecture 2015 韓國造景學會誌 Vol.43 No.3

        Unlike most other planning processes, brownfield planning generally requires a high level of technical and legal expertise due to potential site contamination. To successfully engage in inclusionary decision making, an adaptive collaboration strategy for brownfield planning is therefore critical. This study examines how a communicative planning approach can be used to overcome the challenge of enabling experts from different fields to work alongside lay people from the local community to achieve a properly balanced collaboration in brownfield planning. After identifying appropriate indicators for collaboration through a literature review of established communicative planning theory, these indicators are applied to the brownfield planning process, highlighting critical points of collaboration such as site prioritization, assessment, remediation, and redevelopment throughout. The results suggest the critical need for an adaptive model focusing on three aspects: 1. Facilitation of a balanced dialogue between the experts with social, cultural, and design-based knowledge and the ones with scientific and engineering-based knowledge, 2. Preparation of an appropriate tool for risk communication with the lay people, 3. Development of decision support system for the integration of expert-oriented technical data and public opinion-oriented subjective data. 대부분의 일반적인 계획 과정과 달리, 브라운필드 계획은 잠재된 오염문제로 인한 기술적, 법률적 전문지식을 필요로 한다. 성공적으로 통합적인 의사결정에 관여하기 위해서는 조정된 협력적 계획 모델이 중요하다. 본 연구는 기존의 협력적 계획 접근방식이 브라운 필드 재생에 있어 상반된 분야의 전문가들 및 지역 주민들이 함께 일하며 적절하게 균형 잡힌 협력을 이루어 내기 위한 어려움들을 극복하는 데에 어떻게 효과적으로 적용될 수 있는지 알아보는 것을 목표로 한다. 의사소통적 계획이론 관련 문헌분석을 통해 적절한 지표들을 찾아내어, 네 단계의 브라운 필드 계획과정(대상지 선별, 평가, 정화, 디자인)에 적용시켜 본 후, 중요한 부분들을 짚어내었다. 본 연구의 결과, 크게 세 가지 점에 초점을 맞춘, 브라운 필드 재생에 적합한 협력적 계획 모델의 필요성이 인식되었다(1. 사회, 문화, 디자인 분야와 과학, 기술 분야의 전문가 간의 균형 잡힌 대화의 촉진, 2. 일반주민들과의 잠재적 오염 위험과 관련한 커뮤니케이션을 위한 적절한 방법개발, 3. 전문가 주도의 객관적 데이터와 주민 의견 중심의 주관적 데이터의 통합을 위한 의사결정지원 시스템의 마련).

      • KCI등재

        Disinfection of various materials with 3-(trimethoxysilyl)-propyldimethyloctadecyl ammonium chloride in hatchery facilities

        김유진,Kim Jun-Beom,송창선,Nahm Sang-Soep 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.4

        Objective: Surface disinfection is important in the proper running of livestock farms. However, disinfection of farm equipment and facilities is difficult because they are made of different materials, besides having large surface areas and complex structures. 3-(trimethoxysilyl)-propyldimethyloctadecyl ammonium chloride (Si-QAC) is a quaternary ammonium salt-based disinfectant that attaches to various surfaces by forming covalent bonds and maintains its disinfecting capacity for a considerable time. Our aim was to evaluate the potential use of Si-QAC for disinfection of farm equipment and facilities.Methods: The short- and long-term antimicrobial and antiviral effects of Si-QAC were evaluated in both laboratory and farm settings using modified quantitative assessment method based on the standard operating procedures of the United States Environmental Protection Agency.Results: Si-QAC was highly effective in controlling the growth of the Newcastle disease virus and avian pathogenic Escherichia coli. Electron microscopy revealed that the mechanism underlying the disinfection activity of Si-QAC was associated with its ability to damage the outer membrane of the pathogen cells. In the field test, Si-QAC effectively reduced viral contamination of surfaces of equipment and space.Conclusion: Our results suggest that Si-QAC has great potential as an effective chemical for disinfecting farm equipment and facilities. This disinfectant could retain its disinfection ability longer than other commercial disinfectants and contribute to better farm biosecurity.

      • KCI등재

        규제방식이 창업기업의 진입 및 혁신에 미치는 영향: 한국 사례를 중심으로

        김유진,Kim, Yujin 한국벤처창업학회 2022 벤처창업연구 Vol.17 No.4

        This paper studies the impact of regulatory approaches on innovation and entrepreneurship. As technological progress and environmental changes avail new business opportunities to innovative startups, many governments find it difficult to regulate new and unprecedented businesses promoted by the innovative firms. In order to provide academic and practitional implications on the regulatory design with which to support innovation and entrepreneurship, this paper aims to review classical theories on the demand and supply of regulation as well as empirical research on the impact of regulation on market entry and incentives for innovation. Based on the findings, this paper discusses the recent controversies around the regulatory approaches on new businesses pursued by startups, which are as known as the "positive regulatory approach" vs. "negative regulatory approaches" among practitioners and policy makers in Korea. This paper claims that the Korean context provides an useful opportunity to investigate how the ongoing transition of the once "fast follower" economy into a pacesetter one changes the nature of businesses pursued by firms, investors, and related market players and, accordingly, calls for the changes in the way the government intervenes in markets to regulate businesses of firms. By doing so, this paper sheds light on the role of the government in establishing an entrepreneurial ecosystem where innovative ideas of startups can be tested and nurtured.

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