쇠무릎(Achyranthes japonica Nakai)으로부터 Ecdysteroid 생합성에 관련된 유전자의 분리

김소미,김대운,채현병,조문제,류기증,부경환,진성범,이도승 한국농화학회 2001 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.3

In order to isolate genes involved in ecdysteroids biosynthesis in plants, total RNA was isolated from Achyranthes japonica Nakai, and RT-PCR was performed using degenerate primers selected based on the results of multi-alignment of four cytochrome P450 genes from plants and a putative ecdysone 20-hydroxylase gene from an insect. Fourteen partial cDNA clones showing unique base sequences were obtained, out of which six showed homologies at the levels of nucleotide and amino acid sequences to the other cytochrome P450 genes known to be involved in the ecdysteroid biosynthesis. Of the six clones, four showed relatively high homologies to a putative ecdysone 20-hydroxylase gene isolated from an insect.

Kluyveromyces lactis에 의한 유청으로부터 과일향 성분의 생성

김소미,이형주 한국산업미생물학회 1991 한국미생물·생명공학회지 Vol.19 No.5

유당발효 효모인 Kluyveromyces lactis ATCC 8585를 이용하여 유청에서 과일향 풍미성분의 생산성을 향상시키기 위하여 3차에 걸쳐 NTG를 처리하고 geraniol의 항균력에 내성을 지니는 변이주 중 모균주에 비해 yeasty-flavor는 약하나 fruity-flavor가 강한 변이주를 선발하고 K. lactis 450K라 명명하였다. 3일간 배양시킨 발효액을 pentane-dichloromethane(2:1) 용매로 추출하여 얻은 oleoresin을 gas chromatography로 분석한 결과, 모균주보다 변이주가 더 많은 종류와 양의 향기성분을 생산한 것으로 나타났다. 변이주의 배양액으로부터 잠정적으로 확인된 주요향기성분은 테르펜류로는 myrcenol 등이, 알콜류로는 cis-3-hexenol, n-hexanol, 에스테르류로는 ethyl isovalerate, cis-3-hexenyl n-butyrate, n-amyl-n-hexanoate, phenyl ethyl n-propioate, 케톤류로는 methyl vinyl ketone, 기타 성분으로 vanillin, 3-methylcoumarin 등이었다. To enhance the productivity of fruit flavor compounds from whey by the lactose fermenting yeast, Kluyveromyces lactis ATCC 8585 was treated with N-methyl-N'-nitro-N-nitro-soguanidine(NTG). After the NTG treatments, a mutant showing resistance to antifungal activity of geraniol, and strong fruity but low yeasty flavor was selected and named as K. lactis 450 K. Flavor compounds from 3-day culture broth were extracted with pentane-dichloromethane(2:1) and the concentrated oleoresins were analyzed by gas chromatography. The mutant strain produced more classes and larger amount of flavor compounds than the parent strain. Tentatively identified volatile compounds from the culture of the mutant were: terpenes such as myrcenol; alcohols such as cis-3-hexenol, n-hexanol; esters such as ethyl isovalerate, cis-3-hexenyl n-butyrate, n-amyl-n-hexanoate, phenyl ethyl n-propioate; ketones such as methyl vinyl ketones; other compounds such as vanillin, 3-methylcoumarin.

CTCF as a multifunctional protein in genome regulation and gene expression

김소미,유남경,강봉균 생화학분자생물학회 2015 Experimental and molecular medicine Vol.47 No.-

CCCTC-binding factor (CTCF) is a highly conserved zinc finger protein and is best known as a transcription factor. It can function as a transcriptional activator, a repressor or an insulator protein, blocking the communication between enhancers and promoters. CTCF can also recruit other transcription factors while bound to chromatin domain boundaries. The three-dimensional organization of the eukaryotic genome dictates its function, and CTCF serves as one of the core architectural proteins that help establish this organization. The mapping of CTCF-binding sites in diverse species has revealed that the genome is covered with CTCF-binding sites. Here we briefly describe the diverse roles of CTCF that contribute to genome organization and gene expression.

운전면허용 VR 시뮬레이션을 이용한 가상 주행 체험

김소미,김현식,전인성,김기항 한국정보기술학회 2017 Proceedings of KIIT Conference Vol.2017 No.12

Effect of Selenium Deficiency on Thyroid Hormone and Cardiovascular Diseases in Hemodialysis Patient

김소미,강기수,송현주,정유남,김민정,강민숙,이은주,한수민 한국정맥경장영양학회 2015 한국정맥경장영양학회 학술대회집 Vol.2015 No.-

Introduction: Trace element, selenium deficiency is known to associate with impairment of thyroid hormone and cardiovascular diseases such as coronary artery disease, cardiomyopathy or sudden death. In hemodialysis (HD) patients, various causes may contribute to selenium deficiency, including decreased dietary intake, malabsorption, alteration of metabolism, and removal through dialysis itself. Therefore, we tried to investigate the effect of selenium deficiency on thyroid hormone and cardiovascular diseases in HD patients Methods: This cross-sectional study enrolled 83 end-stage renal disease patients who underwent HD in Jeju National University Hospital. The patients were divided into two groups based on serum selenium levels: 62 patients were normal level and 22 patients were selenium deficient. Thyroid hormones such as TSH, free T4 were measured. And presence of cardiovascular diseases, including ischemic heart disease (IHD), heart failure or cardiomyopathy using echocardiography, coronary computed tomography or coronary angiography were evaluated. Results: There were no significant differences in baseline characteristics including age, sex, presence of diabetes mellitus, hypertension medication between the two groups. Thyroid hormone impairment, including hypothyroidism and subclinical hypothyroidism showed higher tendency in selenium deficient group than that in non- selenium deficient group. ( 27 % vs 10 % P=0.06) The prevalence of ischemic heart disease was significantly higher in selenium deficient group than that in the non-selenium deficient group. ( 59 % vs 21 %, P=0.04 ) But there was no difference in heart failure defined as ejection fraction with below 40 %, and cardiomyopathy between the two groups. All patients with thyroid hormone impairment showed high prevalence of IHD and the coincidence of thyroid impairment and IHD was significantly higher than that in selenium deficient group than that in non-selenium deficient group. ( 18 % vs 4 % , p=0.014) Conclusion: This study showed the significant high prevalence of thyroid hormone impairment and IHD in HD patients with selenium deficiency. Selenium deficiency may be affect heart disease, associating with thyroid hormone impairment

Mind Bomb-2 Regulates Hippocampus-dependent Memory Formation and Synaptic Plasticity

김소미,김태현,이혜련,공영윤,강봉균 대한약리학회 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.6

Notch signaling is a key regulator of neuronal fate during embryonic development, but its function in the adult brain is still largely unknown. Mind bomb-2 (Mib2) is an essential positive regulator of the Notch pathway, which acts in the Notch signal-sending cells. Therefore, genetic deletion of Mib2 in the mouse brain might help understand Notch signaling-mediated cell-cell interactions between neurons and their physiological function. Here we show that deletion of Mib2 in the mouse brain results in impaired hippocampal spatial memory and contextual fear memory. Accordingly, we found impaired hippocampal synaptic plasticity in Mib2 knock-out (KO) mice; however, basal synaptic transmission did not change at the Schaffer collateral-CA1 synapses. Using western blot analysis, we found that the level of cleaved Notch1 was lower in Mib2 KO mice than in wild type (WT) littermates after mild foot shock. Taken together, these data suggest that Mib2 plays a critical role in synaptic plasticity and spatial memory through the Notch signaling pathway.

0 : 스티렌 회분식 현탁중합에서 총괄열전달계수 변화에 따른 폭주반응 해석

김소미,이헌창,장서일,김태옥 한국화학공학회 2007 화학공학의이론과응용 Vol.10 No.2

화학공장에서는 설비의 노후화 등으로 화재, 폭발, 독성물질 누출과 같은 중대산업사고의 발생위험성이 증대되고 있으며, 특히 중합반응공정에서 폭주반응에 의한 사고가 자주 발생되고 있다. 이와 같은 폭주반응은 반응공정이 열적 불안정 상태(thermal instability)에서 발생되므로 화학반응에 대한 열역학과 반응속도론으로 해석할 수 있다. 본 연구는 스티렌 회분식 현탁중합반응에서 폭주반응에 영향을 미치는 인자 중에서 총괄열전달계수의 영향을 해석하기 위해 다양한 실험조건(단량체/분산매 비, 개시제/단량체 비, 단량체질량, 냉각속도, 교반속도 등)에서 반응시간에 따른 온도변화를 측정하여 최대발열온도, 최대발열시간, 최대발열속도 등을 산출하고, 해석하였다. 그리고 자유라디칼 반응속도식과 에너지수지식으로부터 반응온도를 모사하고, 폭주반응에 미치는 총괄열전달계수의 영향을 해석하였다. 그 결과, 반응전화율 변화에 따른 반응조건의 변화를 고려한 총괄열전달계수와 열전달 면적을 사용한 경우에 이론 온도곡선은 실험값과 매우 잘 일치하여 제시된 모델식은 자유라디칼중합반응의 폭주반응을 예측하는데 사용이 가능하였다. 또한 모델식의 모사를 통해 공정제어적인 측면에서 중합반응의 안전적인 조업조건을 결정할 수 있었다.

Silicone 변성 EPDM 방진 고무의 제조와 그 특성

김소미,강두환,임완빈 한국공업화학회 2004 한국공업화학회 연구논문 초록집 Vol.2004 No.-

대가 없는 친절을 베풀어준 아스따

김소미 샘터사 2019 월간 샘터 Vol.588 No.-

The Development of China’s Venture Capital Industry and the Characteristics of its Capital Spatial Configuration

김소미 한국경제지리학회 2017 한국경제지리학회지 Vol.20 No.4

This paper examines the development process of China’s venture capital industry and the characteristics of its capital spatial configuration. The results reveal that the development of China’s venture capital industry has its unique modality, which varies greatly from existing studies, namely, the desynchronized paces of industry development as well as the regional real capital supply and demand trend. More detailed observation shows the Chinese venture capital’s spatial configuration has three main characteristics: first, the separation of the registered location and the actual location of business operation; second, the division and spatial configuration of the venture fund’s capital circulation. To find the relevant factors related to the above phenomena, a correlation analysis was conducted between both market factors and non-market factors with the above capital spatial configuration. The analysis confirmed that besides the market factors, local legislative condition also presents a high correlation with the phenomena.