제사부(第四部): 포스터발표(發表) 연제(演題) : 9. 뉴캣슬병 음수백신(LaSota 주)시 절식과 단수된 육계의 면역성

곽길한,서석열,최인방,류경선,송희종 한국가축위생학회 2001 학술대회논문집 Vol.2001 No.-

전북지역 송아지 설사 유래 병원성 대장균의 병원성 인자 및 다제 내성 패턴

곽길한 ( Kil-han Kwak ),김선민 ( Seon-min Kim ),유영주 ( Yeong-ju Yu ),유정희 ( Jeong-hee Yu ),임미나 ( Mi-na Lim ),장유정 ( Yu-jeong Jang ),허진 ( Jin Hur ) 한국동물위생학회 2021 韓國家畜衛生學會誌 Vol.44 No.4

Pathogenic Escherichia coli (E. coli) is one among the most important agents of diarrhea in calves. From January to December 2021, 108 isolates from feces of calves with diarrhea were investigated for enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), shiga toxin-producing E. coli (STEC), enteroaggregative E. coli (EAEC), and enteroinvasive E. coli (EIEC) using real-time PCR. In addition, the genes for F5, F17 and F41 fimbriae were detected by PCR. The most frequently isolated pathotypes were EPEC/STEC (29 isolates), and ETEC/EPEC/STEC (29 isolates). ETEC/EPEC, and ETEC/STEC were also found in 10 isolates. EPEC, STEC, and ETEC were detected in 13, 11, and 6 respectively. EAEC, and EIEC was not detected. Antimicrobial resistance test was carried out by agar disc diffusion method with 14 antimicrobials. Among 108 pathogenic E. coli isolates, 107 isolates were resistant to at least one of 14 antibiotics used in this study, 99 (91.7%) were resistant to two or more antimicrobials, and a single remarkable isolate was resistant to 14 antimicrobials. The isolates were primarily resistant to penicillins, streptomycin, tetracycline, ceftiofur, Trimethoprim/sulfamethoxazole, Kanamycin, and Ciprofloxacin. The high rate of resistance in pathogenic E. coli, sometimes to multiple drugs, may complicate future options for treating human infections. These results may bu used for diagnosis and therpeitic purposes in calves with diarrhea.

지역아동센터 교사의 아동지도역량강화 프로그램개발을 위한 기초조사

곽길여,이숙 한국지역사회생활과학회 2019 한국지역사회생활과학회지 Vol.30 No.2

This examined the difficulties community children’s center teachers face when teaching children and what behaviors of children they particularly feel difficulty while teaching to provide basic data to develop educational programs for empowering teachers to provide proper guidance for maladaptive children. Surveys were conducted on 431 workers affiliated with community children’s centers in the Jeonnam region and descriptive statistics analysis was conducted. The research results first showed that teachers perceived the psychological, emotional support and life guidance fields to be very important. Second, teachers voiced difficulties due to a lack of competence for dealing with problematic situations of children and particularly in teaching children who were disobedient and absentminded to engage in activities through their experience with teaching maladaptive children. Third, they demanded empowerment education that would improve expert skills and qualifications that could satisfy the various demands of children and parents, such as the skill to handle problematic behavior and communication skills with children and parents. Therefore, this study is significant in that it investigated the actual difficulties experienced by community child center teachers in providing for maladaptive children, thereby acting as a fundamental investigation to explore the needs for an educational program to enhance the capacities for children’s guidance and examine the demands of teachers, such as contents composition, operation hours, and lecture method.

지역아동센터 교사를 위한 부적응행동지도 역량강화교육프로그램 개발 연구

곽길여(Kwak, Gil Yeo),이숙(Lee, Sook) 한국부모놀이치료학회 2020 한국부모놀이치료학회지 Vol.- No.10

The goal of this study is to develop an empowerment education program of maladaptive behavior coaching for teachers working in a community child center and to verify the effect. In order to achieve the goal, total 12 sessions of education program was developed. And the programs were conducted by applying lectures, discussions, hand-out, trials, practices, video clips, and so on. By targeting 9 teachers in a community child center, 120 minutes of education per session were conducted. After each session, the participating teachers had a 1:1「Special Meeting」with a child who had a maladaptive behavior and low self-conception. Also, they had a feedback time on the「Special Meeting」between the teachers and the children. In order to verify the effect of the education program, the teachers received a pre and post examination on teacher’s sense of efficacy, guidance efficacy and child·youth behavior assessment scale while the children had a pre and post self-concept inventory. The collected data was analyzed by applying SPSS 23.0 statistical package. As a result of this study, it was found that teacher’s sense of efficacy and guidance efficacy of teachers was improved and the children’s maladaptive behavior was decreased while the children’s self-conception was improved. This study is meaningful because it provided a new model of teachers’ education program concentrating on training which can strengthen the teachers’ capacity of coaching children’s maladaptive behavior in a community child center to improve children’s psychological·social ability to adapt.

지역아동센터에서 교사의 책읽어주기가 아동의 자아존중감과 독서태도에 미치는 영향

곽길여,공인숙 가정과삶의질학회 2013 가정과삶의질학회 학술발표대회 자료집 Vol.2013 No.11

전북지역 육용종계에서 Mycoplasma gallisepticum과 M. synoviae의 항체 및 유전자 양성률 조사

곽길한 ( Kilhan Kwak ),이흥재 ( Heungiae Lee ),육현수 ( Hyunsu Yuk ),이재욱 ( Jaeuk Lee ),이관호 ( Kwanho Lee ),이영주 ( Young Joo Yi ),이상명 ( Sang Myeong Lee ) 한국가축위생학회 2016 韓國家畜衛生學會誌 Vol.39 No.2

The present study investigated serological and molecular prevalence of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) infection in unvaccinated broiler breeder farms in Jeonbuk providence. An enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) had been used to determine antibody titers against MG and MS, and genome of these pathogens, respectively. Seventy five percent of farms were seropositive for MG and 94% of farms were seropositive for MS. In addition, the rate of antibody positive flocks against MG were 65.3% (32/49), while the rate of positive flocks against MS were 84.2% (80/95). The geometric mean antibody titers were 802.2±626 and 27,726.7±2426 against MG and MS, respectively. Interestingly, none of samples was positive for MG genome by PCR, while 94% (farms), 82% (flocks) and 62.6% (broiler breeder) were positive for MS genome by PCR. These findings suggest that the prevalence of MG or MS infection could be higher than expected. Thus, strict prevention program including vaccination and environmental sanitation should be implemented to avoid disease transmission from breeder to broilers as well as transmission among broilers.

한우 혈액에서 PCR을 이용한 Mycobacterium avium ssp paratuberculosis의 검출

김광현,곽길한,송희종,조정곤 한국임상수의학회 2010 한국임상수의학회지 Vol.27 No.1

Mycobacterium avium ssp paratuberculosis, intracellular bacteria that can cause chronic granulomatous enteritis in cattle, continues to pose significant economic losses and health problem with high prevalence. The purpose of this study is the polymerase chain reaction (PCR)-base strategy for early detection of M. avium ssp paratuberculosis in whole blood. Blood samples were collected from korean cattles in Jeonbuk, Korea. The 16 out of 88 serum samples were detected M. partuberculosis by ELISA. Then samples of infected 8 Korean cattles were amplified by PCR. The PCR amplified targets are 16s rDNA and heat shock protein 65kDa (hsp 65). The 16s rDNA provided a highly sensitive and specific tool for the direct detection of mycobacteria. In addition M. avium was confirmed characteristically by the hsp65. Finally there were sure to M. avium ssp paratuberculosis by IS900 PCR. The restriction fragment length polymorphism was identified by PCR amplifications and subsequence restriction enzyme digestions with Pst I of a hsp65. These results indicate that confirm M. avium with 16s rDNA, hsp65 and a restriction fragment length polymorphism in the hsp65 gene can be seem the other pattern. Therefore, these results can be used for clinical direct detections of M. avium ssp paratuberculosis in whole blood of Korean cattle and also to be used epidemiological researches.

국내 사육 꿩에서 분리된 뉴켓슬병 바이러스의 hemagglutinin-neuraminidase(HN) 유전자의 클론닝과 염기서열 분석

장경수 ( Kyung Soo Chang ),곽길한 ( Kil Han Kwak ),장승익 ( Seung Ik Jang ),김지영 ( Ji Young Kim ),김태용 ( Tae Yong Kim ),송영환 ( Young Hwan Song ),송희종 ( Hee Jong Song ),전무형 ( Moo Hyung Jun ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.3

The gene encoding the HN protein from the CBP-1 strain, a heat stable Newcastle disease virus (NDV) isolated from diseased pheasants in Korea, was characterized by reverse transcriptasepolymerase chain reaction(RT-PCR) and the nucleotide and amino acid sequences were analyzed following cloning of the HN gene. In all of the NDV strains studied, a 1.75 kb size cDNA fragment for the HN gene was generated by RT-PCR and smaller specific band sizes harboring the internal portions of the HN gene were also detected by using four pars of primers. The RT-PCR was sensitive enough to detect viral transcripts when the virus titer was above 25 hemagglutination units. The amplified 1.75 kb cDNA was cloned into a BcamHI site of the pVL1393 Baculo transfer vector. The nucleotide sequences of the 1,758 bp HN gene from the CBP-1 strain were determined by the dye terminator cyclic sequencing method. The gene sequences were compared among the strains of CBP-1, Texas GB, Beaudette C, LaSota, Bl and Ulster. The homology of the CBP-1 HN gene to other HN variants was 97.8% to Texas GB, 98.4% to Beaudette C, 95.4% to LaSota, 95.6% to 131 and 90.2% to Ulster. As the deduced 577 amino acid sequences were compared among the strains, the homology for CBP-1 HN appeared to be 96.7% to Texas GB, 97.9% to Beaudette C, 95.5% to LaSota, 95.5% to BI and 92.7% to Ulster. It was evident that the amino acid sequences included 5 sites for N-asparagine linked glycosylation and 12 cysteine residues. The three conserved leucine residues within the predicted transmembrane domain of the HN protein are amino acid 30, 37 and 44. The three antigenic sites on the HN protein of NDV are amino acids 347 (Glu), 481 (Asn) and 495 (Glu). These data indicate that the genotype of the CBP-1 strain is more closely associated with the strains of Texas GB and Beaudette C than it is for the LaSota, BI and Ulster strains.

전북 익산지역 종계 및 육계에서 전염성빈혈 항체가 조사

엄성심 ( Sung Shim Um ),곽길한 ( Kil Han Kwak ),이정원 ( Joung Won Lee ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.2

From January to November in 2010, a result of chicken infectious anemia virus (CIAV) antibodies ELISA test about 500 number in 25 broiler breeder farms and 500 number in a broiler chickens slaughterhouse from 25 farms in Iksan area, Jeonbuk. The result of 100% (25/25) positive rate in broiler breeder farms group and 98.6% (493/500) positive rate from each individual. In each week-age group, 98.8% of 18~20 weeks and 98.3% of 21~24 weeks showed positive without any significant differences. In slaughterhouse case, broilers from the farms showed 36.0% (9/25) positive rate and each individual showed 20.4% (102/500) positive rate.

마우스에서 살모넬라 감염증 예방을 위한 신개념 Salmonella Typhimurium 불활화 사균체에 최적 adjuvant 선택을 위한 효능 비교 시험

문자영,Enkhsaikhan Ochirkhuyag,김원경,이준우,조영규,곽길한,박병용,허진 한국동물위생학회 2020 韓國家畜衛生學會誌 Vol.43 No.2

This study was carried out to examine a novel inactivated Salmonella Typhimurium (S. Typhimurium) vaccine candidate for protection of mice against salmonellosis by immunization of BALB/c mice using various type adjuvant. The novel type-inactivated vaccine candidate was constructed by adding Chlor-hexidine digluconate solution. BALB/c mice were divided into 6 groups of 15 mice apiece. The mice were intramuscularly (IM) primed at 6 weeks of age and were IM boosted 8 weeks of age. Groups A and B mice were injected with sterile phosphate-buffered saline as controls; group C mice were in-oculated with 5×108 cells/100 μL of formalin-inactivated S. Typhimurium cells and adjuvant ISA70; groups D∼F mice were immunized with 5×108 cells/100 μL of the inactivated vaccine candidate and adjuvant ISA70, adjuvant IMS1313 and adjuvant IMS1313 containing 30 μg/mL of GI24, respectively. All mice (except group A mice) were orally challenged with a virulent S. Typhimurium strain at 10 weeks of age. Mice from groups C-F had significantly increased IgG levels compared to control groups (A-B) mice. The levels of splenocyte IFN-γ and IL-4 in mice of all groups were measured by ELISA, resulting in increased immunity in group F mice compared to those of groups A-E mice. These data suggested that systemic and cell-mediated immune responses were highly induced by IM immunization with the vaccine candidate and adjuvant IMS1313 containing GI24. Furthermore, clinical signs such as death were observed in only 20% of group F mice after virulent Salmonella strain challenge, however, groups B and C (100%), and groups D and E (60%) mice died. This data suggested that mice immunized by intramuscular prime and booster with this vaccine candidate and adjuvant IMS1313 containing GI24 ef-fectively protected mice from salmonellosis.