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정석희,강호정 한국미생물학회 2014 The journal of microbiology Vol.52 No.7
It is important to estimate the true microbial diversities accuratelyfor a comparative microbial diversity analysis amongvarious ecological settings in ecological models. Despitedrastically increasing amounts of 16S rRNA gene targetingpyrosequencing data, sampling and data interpretation forcomparative analysis have not yet been standardized. Formore accurate bacterial diversity analyses, the influences ofsoil heterogeneity and sequence resolution on bacterial diversityestimates were investigated using pyrosequencingdata of oak and pine forest soils with focus on the bacterial16SrRNA gene. Soil bacterial community sets were phylogeneticallyclustered into two separate groups by forest type. Rarefaction curves showed that bacterial communities sequencedfrom the DNA mixtures and the DNAs of the soilmixtures hadmidsize richness compared with other samples. Richness and diversity estimates were highly variable dependingon the sequence read numbers. Bacterial richness estimates(ACE, Chao 1 and Jack) of the forest soils had positivelinear relationships with the sequence read number. Bacterialdiversity estimates (NPShannon, Shannon and the inverseSimpson) of the forest soils were also positively correlatedwith the sequence read number. One-way ANOVA showsthat sequence resolution significantly affected the α-diversityindices (P<0.05), but the soil heterogeneity did not (P>0.05). For an unbiased evaluation, richness and diversity estimatesshould be calculated and compared from subsets of the samesize.
정석희,지상우,강호정,임길재,정영욱 한국자원공학회 2012 한국자원공학회지 Vol.49 No.6
Microbiological reactions by sulfate reducing bacteria and ferrous oxidizing bacteria are critical in the passive treatment of acid mine drainage using constructed wetlands. A lot of research have been done to find better mixing strategy of different substrates to maximize viability of sulfate reducing bacteria and reactor performance. However, research efforts have been poorly made in their microbial ecology comprising community identification and their interactions though they are the main body of acid mine drainage treatment. Currently, to attain high degrees of treatability and sustainability in this wetland-based treatment facility, biotechnological approaches are necessary to improve oxic ponds and successive alkalinity producing ystems, where most of microbiological reactions occur during the treatment. In this paper, we critically reviewed microbial community and physiological functions of sulfate reducing bacteria and ferrous oxidizing bacteria, and suggested perspectives regarding microbiological facility improvement for more sustainable and more efficient passive treatment of acid mine drainage. 소택지를 이용한 광산배수의 자연정화에서 생물학적인 작용은 매우 중요하다. 자연정화시설에서 가장 중요한 미생물의 종류는 황산염환원균(SRB)과 철산화균(FeOB)이 있다. 생물학적 처리에 있어 처리효율에 관련된 물리화학적 분석은 많이 연구되었으나, 생물학적 처리의 처리 주체인 미생물에 대해서는 연구가 미흡한 실정이다. 광산배수처리시설의 생물학적 연구에 있어서 SRB 활성촉진과 지속성 확보를 위한 기질의 배합과 양 조절에 대한 연구, 황산염의 정량을 통해 처리시설에 존재하는 SRB의 활성에 대한 연구 등이 수행되고 있지만, SRB의 정체규명을 위한 분자생물학적 군집분석연구와 미생물간의 상호작용에 대한 생태학적 연구는 현재까지 극히 미미하다. AMD 처리시설의 높은 처리효율과 처리지속성 증진을 위해서, 미생물작용이 활발하게 일어나는 산화조와 알칼리공급조를 생물공학적으로 개선할 필요성이 대두되고 있는 실정이다. 본 논문에서는 자연정화시설의 처리효율과 처리지속성 증진을 목적으로, 황산염환원균과 철산화균의 종류, 기능 및 개선방안에 대하여 생물공학적인 관점에서 기술하였다.
申鉉大,金道淵,金性洙,李鍾秀,鄭錫熙 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 1997 東西醫學硏究所 論文集 Vol.1997 No.-
Iontophoresis is a simple, well-documented method of drug application for medication of tissues. It is possible to medicate electrically any surface tissue with drugs having a positive or negative charge. The technique involves transporting selected ions electrically into a tissue by passing a direct electrical current through a medication solution and the patient, using selected electrode polarity. I am interested in applying herbal medicine to a new method of treatment in musculoskeletal inflammatory conditions instead of western medicine. It is necessary to study on the stability of herbal medicine before application of iontophoresis clinically. Corydalis Tuber, Carthami Flos, and Cnidii Rhizoma were selected and studied. The results are as follows. 1. There were no significant changes in the UV spectrum pattern of these herbal medicines. 2. In the electrical conductivity measurement, the conductivity of Corydalis Tuber ranged from 18.00 to 18.57, that of Carthami Flos ranged from 17.58 to 18.28, and that of Cnidii Rhizoma ranged from 18.15 to 18.94. There were no significant changes in the electrical conductivity in connection with the UV spectrum measurement. 3. In the pH measurement, the pH of all the herbal medicines was measured between 7.06 and 7.07 in the begining, and ranged from 6.909 to 7.601. There were no significant changes in the pH in connection with the UV spectrum measurement. 4. In the quantitative analysis of ions, there were much more anions than cations in Corydalis tuber. 5. In Carthami Flos and Cnidii Rhizoma, more anions were distributed than cations. 6. More anions distributed mainly within all the herbal medicines were CI-, S042- and metals were K, Na. 7. In the HPLC chromatogram, comparing herbal medicines in cold storage with herbal medicines at room temperature, there were no significant changes. Based on these results, it shows that experemental herbal medicines have stability in the study.
정석희,엄지영,장지혜,이재호,조구현,이재석 한국환경생물학회 2015 환경생물 : 환경생물학회지 Vol.33 No.4
Understanding of a carbon storage in a regional scale ecosystem is a very important data for predicting change of global carbon cycle. Therefore, the real data collected in the various ecosystems are a very useful for enhancing accuracy of model prediction. We tried to estimate total accumulated ecosystem carbon in Deogyusan National Park (DNP) with naturally well preserved ecosystem. In DNP, vegetations were classified to four main communities with Quercus mongolica community (12,636.9 ha, 54.8%), Quercus variabilis community (2,987.0 ha, 13.0%), Pinus densiflora community (5,758.0 ha, 25.0%), and Quercus serrata community (402.9 ha,1.7%). Biomass and soil carbons were estimated by the biomass allometric equations based on the DBH and carbon contents of litter and soil (0~30 cm) layers collected in 3 plots (30 cm×30 cm) in each community. The biomass and soil carbons were shown as high value as 1,759,000 tC and 7,776,000 tC, respectively, in Quercus mongolia community in DNP area. In Quercus mongolica, Quercus variabilis, Quercus serrata, Pinus densiflora communities, the accumulated ecosystem carbon were shown 9,536,000 tC, 1,405,000 tC, 147,000 tC, 346,000 tC, respectively. Also, the total ecosystem carbon was estimated with 11,434,000 tC in DNP.