관동화로부터 분리한 sesquiterpene의 nitric oxide synthase 저해활성

정연수,류재하 숙명여자대학교 약학연구소 2004 약학논문집-숙명여자대학교 Vol.20 No.-

Nitric Oxide (NO), derived from L-arginine, is produced by two types of nitric oxide synthases (constitutive NOS: cNOS, inducible NOS: iNOS). The NO produced in large amounts by the iNOS is known to be responsible for the vasodilation and hypotension observed in septic shock and inflammation. The inhibitors of iNOS, thus, may be useful candidates for the treatment of inflammatory diseases accompanied by the overproduction of NO. The activity guided fractionation of the hexane soluble fraction obtained from the methanol extract of Tussilago farfara gave an inhibitory active compound of NO synthesis in LPS-activated macrophages (IC_(50) value was 8.9 μM). The structure of active compound was elucidated as la, 5a-bisacetoxy-8-angeloyloxy-3β, 4β-epoxybisabola-7(14), 10-dien-2-one by spectral analysis. This compound can be a useful candidate for the development of new drugs to treat endotoxemia and inflammation accompanied by the overproduction of NO.

생약추출물 유도형 Nitric Oxide Synthase 저해활성 검색

류재하,이소영,박재현,이화진,장세란,은진희,김남이,정연수,장미경,최영은,이숙현,손행자,안한나,고혜진 숙명여자대학교 약학연구소 2001 약학논문집-숙명여자대학교 Vol.17 No.-

Nitric Oxide (NO), derived from L-arginine, is produced by two types (constitutive and inducible) of nitric oxide synthase (NOS: cNOS and iNOS). The NO produced in large amounts by the iNOS is known to be responsible for the vasodilation and hypotension observed in septic shock and inflammation. The inhibitors of iNOS, thus, may be useful candidate for the treatment of inflammatory diseases accompanied by the overproduction of NO. We prepared alcoholic extracts of herbal drugs which have been used for the treatment of inflammation in oriental medicine. We have screened the inhibitory activity of NO production in lipopolysaccharide (LPS)-activated macrophages after the treatment of these extracts. Among the 81 kinds of extracts of herbal drugs, 34 extracts showed potent inhibitory activity of NO production above 50% at the concentration of 50 (μg/ml. The inhibitory activities of NO production were also evaluated for several solvent fractions at three different concentrations. Especially, hexane soluble fractions of Agrimonia pilosa, Hydrangea serrata, Machilus thunbergii, Prunella vulgaris, Saussurea lappa, Tussilago farfara, and ethyl acetate soluble fractions of Angelica gigas, Ostericum koreanum, Torilis japonica, Perilla frutescence showed moderate activity at 10 and/ or 25 (μg/ml. These fractions are promising candidates for the study of the activity-guided chromatographic purification of active compounds.

AI 조성비 변화에 따른 GaAs/AIGaAs 다중양자우물구조 특성변화 연구

유연희,최정우,임재영,노삼규 慶熙大學校 材料科學技術硏究所 1998 材料科學技術硏究論集 Vol.11 No.-

We manufactured and characterized different sets of GaAs/AlGaAs multiple quantum well structures for the long wavelength infrared photodetection. The samples were grown by MBE. We changed the aluminum composition of barrier region from 0.24 to 0.30 in 0.02 steps, and performed double crystals X-ray diffraction(DCXRD) and the photoluminescence measurements. DCXRD measurements correctly gave the expected aluminum compositions. Photoluminescence measurements revealed that the ground state energy of the well increases with composition as desired, but the change was not very significant.

표정인식을 위한 PCA와 템플릿 정합을 사용한 얼굴 요소 추출

우효정, 이슬기, 김동우, 류성필, 안재형 충북대학교 산업과학기술연구소 2013 산업과학기술연구 논문집 Vol.27 No.2

This paper proposed an extracting algorithm of human facial components for the recognition of facial expressions. First face image is acquired using the Haar-like feature mask from an input image. The face image is divided into two images. One is the upper image including eye and eyebrow. The other is the lower image including mouth and jaw. The extraction of facial components, such as eye and mouth, begins getting eye image and mouth image. Then eigenfaces are produced by the PCA training process with learning images. An eigeneye and an eigenmouth are produced from eigenfaces. Result eye image is obtained by template matching the upper image with the eigeneye, and the mouth image is obtained by template matching the lower image with the eigenmouth. The simulation results show that the proposed method has superior extraction ratio than previous method.

優秀工業系 實科敎員養成을 위한 工業敎育大學 敎育課程 改善에 關한 硏究 : 工業敎育學部를 中心으로

李廷玟,任洪彬,李載元,李來然,金澤基,劉承坤 충남대학교 공업교육연구소 1983 論文集 Vol.6 No.2(A)

This paper is studied to improve the curriculum of the College of Industrial Education of Chungnam National University, which is more helpful for the students to become a competent teacher of technical high school. The thesis includes the curriculum of general, professional, technical subjects and so on. The main conclusions are summarized as follows; 1) The current curriculum of the College of Industrial Education was formed on a wholesale manner following the basis of standard courses for some characterized engineering colleges. But the suggested is conformed to the curriculum system of CNU in credits and hours. 2) The current technical subjects are mainly based on the curriculum of college of engineering, but the newly suggested technical subjects are conformed to the curriculum of technical high school as following that some departments of engineering are founded in the College of Industrial Education. 3) In current curriculum, the ratio of reguired technical subjects to elective technical subjects is 85:15. In suggested curriculum, the ratio of it is largely changed to 45:55 for effective operation of minor cousres. And the lists of the subjects for minor courses are changed from subjects for College of Science to subjects for College of Engineering.

Transarticualr portal를 이용한 주관절의 관절경술

김성재,박인섭,김주영,정재훈,류상욱,천용민 대한견.주관절학회 2003 大韓肩.주關節學會誌 Vol.6 No.2

退行性關節炎 韓方治療에 對한 最近 硏究 動向 : 臨床硏究 方法論을 中心으로 Herbal Medicines in the Treatment of Osteoarthritis in Pubmed and Chinese Medical Journals

서병관,류성룡,이송실,허정은,백용현,이재동,최도영,조윤제,김남재,박동석 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-

Objective : The aim of this study was to review systemically clinical trials on the effectiveness and safety of herbal medicines in the treatment of osteoarthritis. Methods : Computerized literature searches were carried out on seven electronic databases, and hand-searching on some chinese medical journals in library of Kyung Hee Medical Center. Trial data were extracted in a standardized, predefined manner and assessed independently. Results : 1. Thirty reports of clinical trials and two reports of meta-analyses concerning herbal medicine were collected and reviewed Among these reports three medical herbs were applied as topical medicine and others as internal medicine. 2. The western studies established NSAIDs or placebo as their control group. Five chinese reports established formulated herb pill(Ruanshnagshenjin pill) as their control group and Six did not establish a control group at all. 3. ACR was the most highly used diagnostic criteria in the western studies while the Chinese used their official criteria established by their government or the criteria of their text books. 4. 20 reports chose the Lequesne functional index, SHAQ, WOMAC OA index, AIMS, and their own unique scoring system as the criteria of analysing the effect. Others chose clinical symptoms, articular functions, and lab finding as their criteria. 5. 7 single herbs and 19 formulated herbs were studied. Among the formulated herbs, Achyranthes japonica was studied in 10 of the studies and Angelica gigantis Radix in 8, making them the most often studied herbs among the studies.

초등학생의 환경 의식 변화에 관한 종단적 경향성 조사

김영호,류재인,정진수,이혜정,박국태 한국초등과학교육학회 2003 초등과학교육 Vol.22 No.3

The purpose of this study was to survey the change of environmental awareness of elementary school students under the longitudinal-sectional research design. For this study, the questionnaire was used for 599 fifth and sixth grade students living in Seoul, Anseong, and Yangjugun. The results of this survey, compared and analyzed with those of 1987, 1992, 1996, and 2000, were summarized as follows: In the point of environmental awareness, the interest for animal protection and conservation of nature increase but the interest for environmental pollution decreased. There was a high awareness about water pollution, and the awareness about the exhaust of automobile was constantly increasing. Regarding students' knowledge about the environment, their attitudes, and changes of practice, the rate of interest for environmental pollution decreased but the rate of interest for animal protection and conservation of nature increased. As a result of this study, it was found that the students' awareness about the environment varied according to the survey year. By changing the environment education according to this variation, it can be possible to effectively teach students to increase their environmental awareness and develop a habit of environmental protection.

신생아중환자실에서 발생한 Methicillin-Resistant Staphylococcus aureus 집단발생의 분자역학적 조사와 감염관리

이성희,정재심,류선주,김양수,류지소,배직현 대한감염학회 1996 감염 Vol.28 No.2

목 적 : 메티실린내성 포도상구균(MRSA)은 병원감염의 주요 원인균으로 특히 중환자실등 고위험군환자에서 이환율이 높고 집단감염이 자주 발생하여 이의 역학을 밝히기 위한 여러가지 연구가 시행되고 있다. 본 연구에서는 신생아중환자실에서 발생한 MRSA 패혈증 집단발생의 역학조사를 위해 분자역학 방법 중의 하나인 pulsed-field gel electrophoresis(PFGE)를 시행하였으며 그 결과에 의해 감염관리 조치를 취하여 감염을 줄일 수 있었으므로 이에 대한 보고를 하고자 한다. 방 법 : 1995년 3월부터 9월까지 신생아중환자실에 입원하였던 환자 중 MRSA 패혈증을 나타내었던 13명의 환자 중 11명에서 분리된 16 균주를 대상으로 액채배지희석법에 의한 항균제감수성검사와 SmaⅠ제한효소를 이용한 PFGE를 통해 DNA 분석을 실시하였고, 환자기록조사로 MRSA 감염의 시기, 다른 MRSA 감염환자와의 상호관계, 치료에 직접 관여한 의료진과의 관계등을 파악하였다. 감염관리 대책으로는 환자와 의료진의 cohorting을 실시하였으며 손씻기 교육등을 실시하였다. 결 과 : 대부분의 MRSA 군주들은 trimethoprim/sulfamethoxazole. teicoplanin과 vancomycin에만 감수성을 보이고 다제내성균들이었으며 6종류의 양상을 나타내었고, PFGE 결과는 7종류의 양상을 보였다. 감염관리 대책을 도입한 후 4개월간 단 1건만의 MRSA 감염이 있을 뿐이다. 결 론 : PFGE 결과 신생아중환자실에서 발생한 MRSA 감염 집단발생은 외부로부터 유입된 몇가지 균주에 의한 지속적인 감염이었으르로, 환자외 의료진의 cohorting을 실시하고 손씻기 교육을 강조하므로서 감염을 줄일 수 있었다. PFGE는 재현성이 우수하고 균주의 식별이 용이하여 병원감염의 역학적 조사에 유용하게 사용될 수 있을 것으로 사료된다. Background : Methicillin-resistant Staphylococcus aureus(MRSA) is an important cause of nosocomial infection, and infection with this organism can result in considerable morbidity and mortality despite intensive care. Between March and September 1995, 15 episodes of MRSA bacteremia occurred in 13 patients in the neonatal intensive care unit(NICU), which was successfully controlled with the aid of pulsed-field gel electrophoresis(PFGE) for molecular typing of the isolates. Methods : A total of 16 strains of MRSA isolated from 11 patients were typed for antibiogram by microdilution method and chromosomal patterns by PFGE. Infection control measures included in-service teaching sessions, cohorting of patients and nursing staff, and meticulous hand washing. Results : Six patterns of antibiograms and 7 patterns of DNA types were identified in 16 strains indicating the diversity of the outbreak strains. Only one additional episode of MRSA bacteremia occurred in 4 months since the introduction of control measures. Conclusion : The diversity of chromosomal DNA patterns suggested that new strains of MRSA were introduced continuously into the NICU and the infection control measures to prevent its introduction into the NICU were successful in reducing MRSA infection in the unit. Chromosomal DNA typing by PFGE is reproducible and descriminatory, and the which is found very useful in the molecular epidemiologic studies of nosocomial infections.

가압유동층연소로에서 국내무연탄의 황산화물 배출특성

한근희,송용식,류정인,손재익,진경태 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.1

본 연구는 가압유동충연소로에서 국내 무연탄을 연소함에 있어서 SO_2 배출농도에 대하여 고찰하였다. Bench scale PFBC에서 석탁을 연소하는 동안에 탈황제로 식회석을 사용하였으며, 배가스의 배출특성을 조사함에 있어서 SO_2의 제어에 주안점을 두었다. 석탁은 강원도 태백지역에서 채탄된 무연탄이고, 석회석은 강원도 삼척지역에서 얻은 것이다. 실험장치는 층 직경 0.17m, freeboard 직경 0.25m이고, 총 높이가 5m로 이루어졌다. 실험은 운전압력(1-6 atm), 운전온도(850-950℃), 과잉공기(10-30%), Ca/S 몰비(0.8-4.8)의 조건으로 수행하였다. 결과적으로 최대 탈황율을 보인 층(bed)온도는 1, 2 기압일 때 850℃, 4 기압일때 900℃, 6 기압일 때 950℃로 나타났다. 또 탈황율은 모든 실험조건에서 운전압력, Ca/s 몰비, 과잉공기가 증가함에 따라 증가하였다. 국내무연탄의 가압유동충연소시 안정적인 SO_2의 배출을 위하여 각각의 운전압력에서 적정한 Ca/S 몰비는 2 기압 이하일때 Ca/S≥4.8, 4기압일 때 Ca/S=4.8, 6 기압 이상일때 Ca/S≤4.8를 보였다. This study was to investigate the SO_2 emission with Korean anthracite in a PFBC (Pressurized Fluidized Bed Combustor). This work focuses on the control of SO_2, one of the severe air pollutants in flue gas, emission characteristics by injecting sorbent during the combustion of coal in a bench scale PFBC. The coal and limestone used in this work were Taeback anthracite and Samchuck limestone, respectively, in Korea. The effects of operation parameters such as pressure (1-6 atm), bed temperature (850-950℃), excess air ratio(10-30%), and Ca/S mole(0.8-4.8) on desulfurization was investigated in PFBC(0.17m of bed diamether, 0.25m of freeboard diameter and 5.0m height). The bed temperature showing maximum sulfur capture increased from 850℃ at 1 and 2 atm. to 950℃ at 6 atm. with 10% of excess air ratio. Sulfur capture increased as excess air ratio increased at all experimental condition. Both the sulfur capture and the temperature showing maximum sulfur capture increased as operation pressure increased. It was recognized that Ca/S mole ratio higher than 4.8 was needed below 2 atm. and lower Ca/S mole ratio than 4.8 was needed at 4 and 6 atm. for the stable SO_2 emission in combustion of Korean anthracite.