Protein microarray를 이용한 Apin-단백질의 상호작용에 관한 연구

박주철,박선화,김흥중,박종태,윤성호,김지웅,이태연,손호현 대한치과보존학회 2007 Restorative Dentistry & Endodontics Vol.32 No.5

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

Effects of active drinking practices on fluid consumption and sweat rate while exercising in a hot environment

( Youn Sun Son ),( Bong Yeon Hwang ),( Dae Taek Lee ),( Yoon Jung Bae ) 한국운동영양학회 2014 Physical Activity and Nutrition (Phys Act Nutr) Vol.18 No.2

Youn Sun Son, Bong Yeon Hwang, Dae Taek Lee and Yoon Jung Bae. Effects of active drinking practices on fluid consumptionand sweat rate while exercising in a hot environment. JENB., Vol. 18, No. 2, pp.215-223, 2014 [Purpose]To examine the effectsof active drinking practices on fluid consumption and sweat rate while exercising in a hot environment. [Methods]Nine mencompleted two experiments. Each consisted of 3 phases: pre-testing (pre), training period, and post-testing (post). During testing,the subjects ran on a treadmill at a moderate intensity for 90 min at 39 ± 1℃ followed by a 3-h recovery. They drank ad libitum. During training, they ran for 90 min for 7 days while either drinking actively (AH, 150% of weight loss) or passively (PH,50% of weight loss). [Results]The actual volume consumed in training was three times greater during AH than during PH. Inpost during AH, the volume of drinking was two times greater than pre (1592 ± 953 and 855 ± 551 mL, respectively; p < 0.05). No difference in volume consumption during PH between pre and post was found. The sweat loss during exercise was greaterin post (1377 ± 956 mL) than in pre (558 ± 642 mL) during AH (p < 0.05), but not during PH. Rectal temperature and heartrate decreased after training. Serum osmolality following exercise were not different than the baseline or between the conditions. [Conclusion]Active drinking practices while exercising in a hot environment induced greater voluntary fluid intake and sweatloss. [Keyword]voluntary intake, rehydration, thermoregulation.

Associations between Health Literacy, Cancer-Related Knowledge, and Preventive Health Behaviors in Community-Dwelling Korean Adults

Son, Youn-Jung,Kim, Sun-Hee,Kim, Gi Yon,Son, Heesook Informa UK (TaylorFrancis) 2017 JOURNAL OF HEALTH COMMUNICATION Vol.22 No.12

T-REX 삼축 가속도계의 다양한 부착위치에 따른 신체활동량 추정 타당도 검증

손윤선(Youn Sun Son),이종도(Chong Do Lee),김기언(Key Eon Kim),이대택(Dae Teak Lee),황봉연(Bong Yeon Hwang),김종광(Jong Kwang Kim) 한국체육측정평가학회 2016 한국체육측정평가학회지 Vol.18 No.2

이 연구의 목적은 새로 개발된 삼축 가속도계인 T-REX의 신체활동량 추정에 대한 타당도를 준거 도구(가스호흡분석기)와 참고 도구(가속도계)와의 비교를 통해 검증하는 것이다. 건강한 20대 남녀 50명은 운동자각인지도 12-14 수준으로 훌라후프, 스텝박스, 줄넘기와 제자리 걷기를 수행하였다. 대상자들은 운동을 수행하는 동안 T-REX(발목, 상완, 가슴, 허리, 손목 위치)와, ActiGraph(허리 위치), Actical(허리, 가슴 위치)을 착용하였으며, 산소섭취량이 동시에 측정 되었다. 타당성은 피어슨 상관계수로 분석하였다. T-REX는 발목, 가슴, 허리 위치에서 스텝박스 운동과(r=.83-.91) 훌라후프 운동 시 상완과 손목 위치에서(r=.82-.84) 산소섭취량과 매우 강한 상관관계를 보였으며, 제자리 걷기 운동 시에는 상완과 손목에서 보통이상의 상관관계(r=.63-.71)를 나타내었다. T-REX는 두 종류의 참고도구와 비교했을 때 산소섭취량과 더 높은 상관관계를 보여, 신체활동 검사 도구로 충분히 상용화될 수 있을 것으로 판단된다. This study examined the validity of the T-REX accelerometer to measure physical activity in men and women comparing with Actigraph and Actical. Fifty healthy men and women completed four different types of exercise (step box, jump rope, Hula hoop, and on-site walking) at a perceived exertion intensity rated 12-14. Oxygen uptake (VO₂) were assessed from all participants while assessing physical activity using T-REX (attached at the ankle, arm, chest, waist, and wrist) and, simultaneously, Actigraph (attached at waist) and Actical (attached at the waist and chest), respectively. VO₂ was measured using the Cosmed K4b2 portablesystem. There were very strong to strong associations between T-REX and VO₂ measured during Step box activity (T-REXs were attached at the ankle (r=.91), chest (r=.83), and waist (r=.86). There were also strong associations between T-REX and VO₂ measured during Hula hoop activity (T-REXs were attached to the arm (r=.82) and wrist (r=.84). Also, there were significant associations between T-REX and VO₂ measured during on-site walking at five locations (r=.63-.71). When compared with ActiGraph and Actical, T-REX showed higher associations with VO₂, which indicates a superior instrument to measure physical activity. The T-REX has high validity with VO₂ to measure physical activity including Step box activity in three locations (ankle, chest, and waist), Hula hoop activity in two locations (arm, wrist), and on-site walking in one location (ankle).

국내 수면 질 관련 양적 연구동향 및 측정도구 분석

손연정(Son Youn-Jung),박진희(Park Jin-Hee),윤현경(Youn Heun-Keung),김선희(Kim Sun-Hee) 한국성인간호학회 2009 성인간호학회지 Vol.21 No.6

Effects of Chungkukjang extract on growth hormone secretion and their related signaling pathway in pituitary originated cells and SD rats

Sun Il Choi,In Sik Hwang,Jun Seo Goo,ji Eun Kim,Hye Ryun Lee,Young Ju Lee,Mon Hwa Kwak,Hong Joo Son,Dong Sup Kim,Kyu Min Park,Dae Youn Hwang 한국실험동물학회 2012 한국실험동물학회 학술발표대회 논문집 Vol.2012 No.8

Effects of Chungkookjang Extract on Growth Hormone Secretion from GH3 Mouse Pituitary Cell and Growth Hormone Receptor Signaling Pathway

Sun Il Choi(최선일),Ji Eun Kim(김지은),In Sik Hwang(황인식),Hye Ryun Lee(이혜련),Young Ju Lee(이영주),Hong Joo Son(손홍주),Dong Seob Kim(김동섭),Kyu Min Park(박규민),Dae Youn Hwang(황대연) 한국생명과학회 2012 생명과학회지 Vol.22 No.9

뇌하수체 전엽에서 성장호르몬의 생산과 분비는 세포의 분열과 분화 그리고 이동을 조절하는 몇 가지 천연물질에 의해 유도된다. 따라서 발효과정을 통해 제조된 청국장이 성장호르몬의 대사에 미치는 영향을 평가하기 위하여 성장호르몬 분비능과 반응성을 뇌하수체 세포와 성장호르몬 표적세포에서 관찰하였다. 6가지 종류의 콩 품종으로 제조된 청국장 추출물 중에서, 대원, 대풍, 태광의 3종류 청국장 추출물은 5 mg/ml 농도에서 GH3 세포로부터 성장호르몬의 분비를 촉진하였다. 비록 세포 생존능은 이러한 추출물에 의해 유의적인 변화가 유도되지 않았으나, 성장호르몬의 분비량은 청국장 추출물의 농도에 의존적으로 증가하였다. 또한 성장호르몬의 표적 기관으로부터 유래된 MG63과 HepG2 세포는 GH3로부터 수집된 조건적 배양액에 의해 유의적으로 활성화되었다. 또한 이러한 세포에서 STAT5 발현은 대원 청국장 추출물을 처리 후, 15분 혹은 30분부터 세포질에서 유의적으로 증가하였으며, p-STAT5는 핵에서 30분 혹은 60분부터 증가하였다. 따라서 이러한 결과는 3가지 종류의 청국장 추출물은 성장호르몬의 분비를 촉진시키며, 청국장의 조건적 배양액은 성장호르몬 표적세포에서 신호전달을 유도함을 제시하고 있다. The production and secretion of growth hormone (GH) in the anterior pituitary gland can be induced by several natural products to control cell proliferation, differentiation, and migration. To investigate whether Chungkookjang (CKJ) produced by the fermentation process affects GH-related metabolism, the secretion and the response of GH were observed in pituitary cells and GH target cells. Among six CKJs manufactured by different strains of glycine max, only three CKJs, including Daewon (DW), Daepung (DP), and Taegwang (TG), induced GH secretion from GH3 cells at 5.0 mg/ml concentration. There were no significant changes detected in the viability of any of the cells treated with these CKJs. In addition, the increase in GH secretion from the GH3 cells was dependent on the concentration of the three types of CKJs. The proliferation of cell lines, including MG63 and HepG2 cells, that originated from those derived from the GH target organs was significantly activated by treatment with the GH-containing conditional medium (GCM) harvested from the three CKJ-treated GH3 cells, although their induction rate was different from each other. In these cells, p-STAT5 was maximally translocated into the nucleus of MG63 cells 30 min after DW treatment, while it was translocated in HepG2 cells at 60 min. These results suggest that these three types of CKJ could enhance the secretion of GH, as well as the GCM-derived response, in the two target organs.

Direct effect of protein kinase C inhibitors on cardiovascular ion channels

( Youn Kyoung Son ),( Da Hye Hong ),( Dae Joong Kim ),( Amy L Firth ),( Won Sun Park ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.9

Protein kinase C (PKC) is a central enzyme that modulates numerous biological functions. For this reason, specific PKC inhibitors/activators are required to study PKC-related signaling mechanisms. To date, although many PKC inhibitors have been developed, they are limited by poor selectivity and nonspecificity. In this review, we focus on the nonspecific actions of PKC inhibitors on cardiovascular ion channels in addition to their PKC-inhibiting functions. The aim of this paper is to urge caution when using PKC inhibitors to block PKC function. This information may help to better understand PKC-related physiological/biochemical studies. [BMB reports 2011; 44(9): 559-565]

Effects of Red Liriope platyphylla on NGF secretion ability, NGF receptor signaling pathway and γ-secretase components in NSE/hAPPsw transgenic mice expressing Alzheimer’s Disease

Sun-Il Choi,Jun-Seo Goo,Ji-Eun Kim,In-Sik Hwang,Hye-Ryun Lee,Young-Ju Lee,Hong-Joo Son,Hee-Seob Lee,Jong-Sup Lee,Dae-Youn Hwang 한국실험동물학회 2012 Laboratory Animal Research Vol.28 No.3

Liriope platyphylla (LP) has long been regarded as a curative herb for the treatment of diabetes, asthma, and neurodegenerative disorders. To examine the therapeutic effects of Red LP (RLP) manufactured by steaming process on neurodegenerative disorders, significant alteration of the key factors influencing Alzheimer’s Disease (AD) was detected in NSE/hAPPsw transgenic (Tg) mice after RLP treatment. The concentration of nerve growth factor (NGF) in serum increased in RLP-treated NSE/hAPPsw Tg mice compared with vehicle-treated Tg mice. However, downstream effectors of the NGF receptor signaling pathway, including TrkA and p75NTR proteins, were suppressed in RLP-treated NSE/hAPPsw Tg mice. Especially, Tg mice showed decreased levels of TrkA, p75NTR, and RhoA expression. Production of Aβ-42 peptides was lower in RLP-treated NSE/hAPPsw Tg mice than in vehicle-treated Tg mice. Further, analysis of γ-secretase components showed that Aβ-42 peptide expression was downregulated. Of the four components, the expression of APH-1 and Nicastrin (NCT) decreased in RLP-treated NSE/hAPPsw Tg mice, whereas expression of PS-2 and Pen-2 was maintained or increased within the same group. Overall, these results suggest that RLP can help relieve neurodegenerative diseases, especially AD, through upregulation of NGF secretion ability, activation of NGF signaling pathway, downregulation of Aβ-42 peptide deposition, and alteration of γ-secretase components.