민간단체 소속 자원봉사자의 식품안전에 대한 인식 및 관련 교육에 대한 요구

김은선 충북대학교 교육대학원 2016 국내석사

본 연구는 민간단체 소속 자원봉사자들에게 필요한 식품안전 정보를 제공하기위한 교육방법 및 교육프로그램 개발을 위한 기초자료를 얻기 위해 식품안전 교육 경험과 식품안전에 대한 인식 및 관련 교육 요구도를 알아보고자 청주지역에 거주하는 민간단체 소속 자원봉사자를 대상으로 설문조사를 실시하였으며 결과는 다음과 같다. 첫째, 자원봉사자의 일반적인 특성을 살펴보면, 여자 71.1%, 남자 28.9%로 여자가 남자보다 두 배 이상의 응답률을 보였으며, 연령별로는 ‘50대’가 50.8%, 최종학력별로는 ‘고졸’이 53.6%, 직업별로는 ‘직장인’이 52.2%, 가족 수별로는 ‘4인’에서 31.9%, 월 소득별로는 ‘300~499만원’이 31.9%, 봉사활동 총 경력별로는 ‘10년 미만’이 52.2%, 재난 구호활동 참여횟수별로는 ‘1회 ~4회’가 44.9%, 향후 봉사활동 참여계획별로는 ‘개인사정에 따라서 참여’가 68.1%에서 가장 높게 나타났다. 식품안전 교육 경험 유무에 대해서는 ‘유’는 46.3%, ‘무’는 53.7%로 나타났다. 일반적 특성에 대해 식품안전 교육 경험 유무에 따라 분석한 결과 재난 구호활동 참여횟수(p<0.05)에서 유의적인 차이가 나타났으며, 그 외의 일반사항에서는 유의적인 차이가 나타나지 않았다. 식품안전 교육 경험이 있는 사람은 연1회 미만으로 위생관리에 대한 전반적인 내용을 소집형태에서 구두로 전달 받았다는 응답이 가장 높은 것으로 나타났다. 둘째, 식품안전에 관한 인식, 관심, 지식, 행동 정도를 나타낸 결과이다. 식품안전에 대해 안전하다고 인식하는 사람은 37.6%, 불안전 하다고 인식 하는 사람은 7.4%로 5점 만점에 3.3점으로 나타났으며, 식품안전 교육 경험 유무에 따라 유의적인 차이가 나타나지 않았다. 식품안전에 관한 관심이 높은 사람은 43.6%, 관심이 낮은 사람은 4.1%로 5점 만점에 3.5점으로 나타났으며, 식품안전 교육 경험 유무에 따라 유의적인 차이가 나타나지 않았다. 식품안전에 관한 지식도는 20점 만점에 14점 정도로 100점 만점으로 환산하면 약 70점 정도이다. 식품안전 교육 경험 유무에 따라 유의적인 차이가 나타나지 않았다. 식품안전에 관한 행동점수는 총점 30점 만점에 20.8점의 점수 보였으며, 식품안전 교육 경험 유무에 따라 6문항 중 ‘캔 제품 구매 시 손상여부 확인’(p<0.01)과 ‘조리 전 손을 씻을 때 손목 위, 손가락 사이도 거품을 내서 씻기’(p<0.05)에서 유의적인 차이가 나타났다. 식품안전 교육을 받아본 경험이 있는 사람이 식품안전과 관련된 행동에 더 노력하며, 행동점수도 높은 것으로 나타났다. 셋째, 식품안전 정보획득이 쉽다고 인식하는 사람은 10.7%, 쉽지 않다고 인식하는 사람은 16.8%로 조사되었다. 식품안전 정보를 얻는 수단에 대해서는 응답자의 51.7%가 ‘대중매체’를 통해 얻는 것으로 나타났다. 소속단체 및 전문 강사를 통한 교육의 빈도는 낮게 조사되었다. 식품안전 정보획득의 용이성과 정보를 얻는 수단에 대해 식품안전 교육 경험 유무에 따라 유의적인 차이가 나타나지 않았다. 넷째, 향후 식품안전에 관련된 교육의 필요성에 필요하다고 인식하는 사람은 75.9%, 필요하지 않다고 인식하는 사람은 1.3%로 나타났으며, 식품안전 교육 경험 유무(p<0.01)에 따라 유의적인 차이가 나타났다. 식품안전에 관한 교육을 받은 경험이 있는 사람이 향후 식품안전에 관련된 교육에 대한 요구도가 더 높게 나타났으며, 전문 강사를 통한 교육을 가장 선호하는 것으로 조사되었다.

수면장애 또는 배변장애 유무에 따른 성인의 식품섭취빈도 및 영양소 섭취량 비교

조혜란 충북대학교 대학원 2013 국내석사

This study was assessed the comparison of food consumption frequency and nutrient intake in adults with or without sleep disorder and functional gastrointestinal disorders. Among 218 adults, 83(38.1%) had not only sleep disorder but also functional gastrointestinal disorder, and 63(28.9%) had only sleep order, and 24(11.0%) had only functional gastrointestinal disorder, and 48(22.0%) were normal group. According to the analysis of the correlation between sleep order and functional gastrointestinal disorder was a significant small positive correlation(r=0.221, p<0.001). The prevalence of insomnia, daytime sleepiness, sleep apnea, irritable bowel syndrome, and constipation were 81(37.2%), 68(31.2%), 74(33.9%), 81(37.2%), and 71(32.6) respectively. The dietary habits of sleep disorder and functional gastrointestinal disorder groups were overeating, and sleep disorder group was eating too fast, after adjusting for confounding factors such as gender, age, marital status, and educational level. Compared with sleep disorder or non-sleep disorder on frequency of food intake per week, sleep disorder group was consumed a lower frequency of multigrain rice, pork, anchovy, bean, radish, pepper, orange juice, and a higher frequency of bread, persimmon, carbonated drink after adjusting for confounding factors. And Compared with functional gastrointestinal disorder or non-functional gastro -intestinal disorder on frequency of food intake per week, functional gastro -intestinal disorder group was consumed a lower frequency of cereal, pork, ham, anchovy, bean, radish, pepper, persimmon, ramen, and a higher frequency of noodles, bread, cooking oil, carbonated drink, orange juice after adjusting for confounding factors. Comparison of micronutrients intakes per day shows that sleep disorder group intakes less carbohydrate, vegetable protein, vitamin B6, phosphorus, natrium, zinc, potassium than non-sleep disorder group after adjusting for confounding factors. And Comparison of micronutrients intakes per day shows that functional gastrointestinal disorder group intakes lower vitamin A and higher fat, animal fat, vitamin B6, zinc, potassium than non-functional gastrointestinal disorder group after adjusting for confounding factors. Also comparison on nutrition intake and dietary reference intakes for koreans(2010) shows that with or without sleep disorder and non-sleep disorder intake lower carbohydrate, natrium, potassium and higher vitamin B6, zinc than recommended nutrients. Likewise with or without functional gastrointestinal disorder and non-functional gastrointestinal disorder intake lower potassium and vitamin B6, zinc than recommended nutrients. And non-functional gastrointestinal disorder group intake only lower vitamin A than recommended nutrients. In conclusion, sleep order and functional gastrointestinal group are interact with each other. The interpretation of the relevance of sleep disorder or functional gastrointestinal disorder with dietary habits, food intake is necessary to be considering the various factors.

테프 에탄올 추출물의 암세포 증식, 전이 및 혈관신생 억제 효과

서진아 충북대학교 2020 국내석사

본 연구에서는 생과 건열처리된 테프 에탄올 추출물이 암세포의 주요 특성인 무절제적인 증식과 활성화된 전이에 미치는 영향을 HCT116 인간 유래 대장암세포주와 H1299 폐암세포주를 이용하여, 혈관신생에 미치는 영향을 인간 제대정맥 내피세포와 닭 배아 장뇨막 모델을 이용하여 각각 비교·분석하고자 하였다. 1. RTE와 HTE는 대장암세포와 폐암세포의 증식을 억제하였고, HTE는 RTE보다 증식 억제활성이 더 높았는데, 이는 G2/M기 arrest 및 apoptosis 유도 활성과 관련된 것으로 생각된다. 또한 건열처리에 의해 테프의 암세포 증식 억제활성이 증가되는 것으로 생각된다. 2. RTE와 HTE는 대장암세포와 폐암세포의 침윤, 이동, 부착을 억제하였고, HTE는 RTE보다 침윤, 이동, 부착 억제활성이 더 높았다. 이와 같은 결과로 보아 테프 추출물은 in vitro 수준에서 전이 억제활성을 가지는 것으로 생각되고, 건열처리에 의해 테프의 암세포 전이 억제활성이 증가되는 것으로 생각된다. 3. RTE와 HTE는 HUVEC의 증식, 침윤, 이동, 부착, tube 형성을 억제하고, CAM 모델에서 혈관 형성을 억제하였다. HTE는 RTE보다 이러한 억제활성이 더 높았다. 이와 같은 결과로 보아 테프 추출물은 in vitro 및 ex vivo 수준에서 혈관신생 억제활성을 가지는 것으로 생각되고, 건열처리에 의해 테프의 혈관신생 억제활성이 증가되는 것으로 생각된다. The current study aimed to investigate inhibitory activities of Eragrostis tef L. (teff), a gluten-free grain, consumed as the major staple food in Ethiopia, against important characteristics of cancer cells, including unrestricted growth, resisted apoptosis, pathological angiogenesis and activated metastasis, in human cancer cells, and human umbilical vein endothelial cells (HUVEC), and chicken chorioallantoic membrane (CAM) model. Raw and heated teff were freeze-dried and extracted with 80% ethanol (RTE and HTE, respectively). Treatment of HCT116 colon cancer cells and H1299 lung cancer cells with RTE and HTE at the concentrations of 100-1,000 μg/mL for 24-72 h dose- and time-dependently inhibited the cell growth. RTE and HTE also increased the number of cells at sub-G1 and G2/M phases, indicating their apoptosis-inducing and G2/M-arresting activities. In addition, RTE and HTE at their non-cytotoxic concentrations effectively inhibited invasion, migration, and adhesion. In all of the assays, HTE produced more prominent effects than RTE. In HUVEC, treatment with RTE and HTE resulted in dose-dependent inhibition of cell growth, invasion, migration, adhesion to fibronectin, monocyte adhesion, and tube formation. In CAM model, RTE and HTE significantly suppressed the branching of new capillaries from the existing basal vessels. In both HUVEC and CAM model, HTE produced more prominent effects than RTE. Taken together, these results suggest that teff possesses anti-cancer activities against colon and lung cancers and anti-angiogenic activities and that heating may enhance these activities. Further studies are needed to determine if similar effects are reproduced in vivo and which compositional changes are responsible for the increased activities of HTE.

시판 과채류 음료의 당 함량과 충청지역 20대 직장인의 과채류 음료 소비 실태 및 당과 영양성분표시에 대한 인식

문주윤 충북대학교 2022 국내석사

The purposes of this study are to compare the sugar content of some commercially available fruit and vegetable beverages, and investigate consumption of the fruit and vegetable beverages and perception on the sugar and nutrition label among office workers in their 20s in Chungcheong area. The main results of this study are as follows. First, 159 of commercially available fruit and vegetable beverages were used in the study. In the products with a nutrition label (n=114), the sugar content per 100 g was 8.1 ± 0.3 g, and the sugar content per serving exceeded 25% of the WHO recommended standard for sugar intake. Among the products with a nutrition label (n=114), there are products with a compulsory label and with an autonomous label. In the products with a compulsory label (n=75), the sugar content per 100 g was 8.6 ± 0.3 g, and the sugar content per serving exceeded 28% of the WHO recommended standard for sugar intake. In the products with an autonomous label, the sugar content per 100 g was 7.1 ± 0.7 g, and the sugar content per serving exceeded 19% of the WHO recommended standard for sugar intake. In the products without a nutrition label (n=45), the sugar content per 100 g was 10.5 ± 0.3 g, and the sugar content per serving exceeded 35% of the WHO recommended standard for sugar intake. The sugar content was higher in the order of products without a nutrition label, with a compulsory label, and with an autonomous label. Second, as a result of a survey of 191 office workers in their 20s in Chungcheong area, When purchasing fruit and vegetable beverages, the survey subjects mainly used supermarkets and convenience stores. And 60% of the survey subjects did not check the nutrition label. The main reason for not checking the nutrition label was ‘not interested’. When making a purchase decision, the importance of the nutrition label was above average, and 'taste' was the most important consideration among various considerations. Concerns about intake, the response rate of 'high sugar content' was high. In addition, the average knowledge score for sugar and nutrition label was about 6 points (out of 12 points). Among the knowledge questions, the correct answers to the question about the WHO recommended standard and the question about the product requiring a compulsory label were low (18.3%, 12.6%). The awareness level was moderate, and awareness of the question of the need for nutrition label for fruit and vegetable beverages that are not compulsory to nutrition label was high. As a result of these results, this study suggested the necessity of nutrition label through the nutrition label status of some commercially available fruit and vegetable beverages and the comparison of the sugar content of products with a nutrition label and without a nutrition label. In addition, it is thought that the survey results will provide basic data on dietary education for desirable intake habits.

엽채류 잎 추출물에 의한 in vitro 항암 효과

곽영은 충북대학교 2014 국내석사

The aim of the study was to investigate anti-cancer activities of green mustard leaf (GML), red mustard leaf (RML), bean leaf (BL), and perilla leaf (PL) extracts in vitro. GML, RML, BL, and PL extracts significantly inhibited cell growth (by 30.9-91.5% in HCT116 human colon cancer cells and by 45.4-86.0% in H1299 human lung cancer cells at the concentration range of 150-600 µg/ml) and anchorage-independent cell growth (by 39.4-100% in HCT116 cells and by 27.3-100% in H1299 cells at 600 µg/ml concentration). GML, RML, BL, and PL extracts also changed nucleus morphology and increased nuclear staining intensity (by 55.7-121.5% in HCT116 cells and by 31.6-62.5% in H1299 cells at the concentration range of 150-600 µg/ml), indicating apoptosis-inducing activities. GML, RML, BL, and PL extracts also inhibited cell migration (by 24.5-66.7% in HCT116 cells and by 25.8-75.5% in H1299 cells at the concentration range of 150-600 µg/ml) and attachment (by 13.5-87.1 in HCT116 cells and by 48.8% in H1299 cells at the concentration range of 150-600 µg/ml). These results demonstrate anti-cancer activities of GML, RML, BL, and PL extracts in vitro. 본 연구에서는 들깻잎, 콩잎, 청겨자잎, 적겨자잎 추출물의 암세포 증식 억제활성, 부착 비의존적 증식 억제 활성, apoptosis 유도 활성, 세포 주기 변화, 이동성 억제 활성, 접착 억제 활성 등의 항암 효과에 대하여 HCT116 인간 대장암 세포주와 H1299 인간 폐암 세포주를 이용하여 in vitro 수준에서 조사하였다. 1. HCT116 대장암 세포 또는 H1299 폐암 세포에 150, 300, 또는 600 µg/ml 농도의 들깻잎, 콩잎, 겨자잎 추출물을 48시간, 72시간, 또는 96시간 동안 처리하였을 때, 대조구 생존 대비 8.5-69.1%의 활성을 보여 유의적인 세포 증식 억제 활성을 보였다(p<0.05). 콩잎, 겨자잎 추출물에 의한 HCT116 대장암 세포 또는 H1299 폐암 세포의 부착 비의존적 증식 억제 활성은 대조구 활성 대비 2.0-2.7%로, 모든 시료에서 유의적인 세포 증식 억제 활성을 보였다(p<0.05). 특히 들깻잎 추출물에서는 100%의 부착 비의존적 증식 억제 활성을 보였다(p<0.05). 2. HCT116 대장암 세포 또는 H1299 폐암 세포에 150, 300, 또는 600 µg/ml 농도의 겨자잎, 들깻잎, 콩잎 추출물을 처리하였을 때 세포의 핵의 염색 강도가 대조구에 비해 유의적으로 증가하였다(대조구 대비 131.6-221.5%, p<0.05). 이는 잎채소 추출물이 부분적으로 apoptosis를 유도하기 때문인 것으로 생각된다. 3. 150, 300, 또는 600 µg/ml 농도의 들깻잎, 콩잎, 겨자잎 추출물에 의한 HCT116 세포 또는 H1299 폐암 세포의 이동성 억제 활성은 대조구 활성 대비 24.5-75.5%를 나타내었다. 특히 600 µg/ml 농도의 모든 잎채소 추출물에서 유의적인 이동성 억제 활성을 보였다(대조구 활성 대비 24.5-64.4%, p<0.05). 4. HCT116 세포에 150, 300, 또는 600 µg/ml 농도의 들깻잎, 콩잎, 겨자잎 추출물을 2시간 또는 18시간 처리하였을 때 모든 시간 및 모든 농도에서 유의적인 접착 억제 활성을 보였다(12.9-86.5%, p<0.05). 한편 H1299 세포에서는 150, 300, 또는 600 µg/ml 농도의 들깻잎, 콩잎, 겨자잎 추출물을 2시간 동안 처리하였을 때 최대 48.8%의 접착 억제 활성을 보였다. 앞으로 in vitro 수준에서 나타난 이와 같은 들깻잎, 콩잎, 겨자잎 추출물의 활성이 in vivo 수준에서도 재현될 수 있는지 여부가 확인되어야 할 것으로 생각되며, 이러한 항암 활성의 세부 작용기전에 대한 연구가 필요할 것으로 생각된다. 또한 항암활성에 중요한 역할을 하는 들깻잎, 콩잎, 겨자잎의 주요 기능 성분들이 분리・동정되어야 할 것으로 생각된다.

활성화된 RAW 264.7 대식세포와 궤양성 대장염 마우스 모델에서 엽채류 잎의 항염증 효과

이유나 충북대학교 2014 국내석사

The aim of the present study was to investigate anti-inflammatory activities of leaf vegetables in RAW 264.7 macrophages and an ulcerative colitis mouse model. In lipopolysaccharide-treated RAW 264.7 cells, the treatment with ethanol extract of perilla leaf at the concentrations of 62.5, 125, and 250 μg/mL decreased nitric oxide (NO) and prostaglandin E(PGE) levels by 12-48% (p<0.01). The treatment with bean leaf extract at the concentrations of 125 and 250 μg/mL decreased NO and PGE levels by 16.5-32% (p<0.05). The treatment with squash leaf ethanol extract does not affect to NO levels but decreased PGE levels by 27-45% at the 62.5, 125, and 250 μg/mL concentrations (p<0.01). In the dextran sodium sulfate-treated ulcerative colitis mice, the treatment with 2.5%, 5%, and 10% perilla leaf on diet decreased the disease activity index by 50-88% (p<0.01). The dietary treatment with 2.5%, 5%, and 10% perilla leaf significantly decreased colonic levels of cyclooxygenase-2 (by 56-69%; p<0.05). The dietary treatment with 10% perilla leaf also decreased colonic interleukin-6 and leukotrien B levels (by 26%; p<0.05). These results indicate that perilla leaf exerts anti-inflammatory activities both in vitro and in vivo.

조리법에 따른 참깻잎의 항산화 및 in vitro 항암 활성

김서윤 충북대학교 2015 국내석사

The aim of the study was to investigate the effect of cooking on antioxidant activities of sesame leaves (Seasamum indicum L.; SL) and their inhibitory activities against major characteristics of cancer cells. Raw, blanched, steamed, and roasted leaves were freeze-dried and extracted with 70% ethanol. Antioxidant content, such as total polyphenol (TP), total flavonoid (TF), carotenoid (CT), and chlorophyll (CL) levels, and antioxidant activities, such as radical-scavenging activity (RSA) and ferric reducing antioxidant power (FRAP), were determined. TP, TF, CT, CL levels of raw SL were 18 mg GAE/100 g, 65 mg QE/100 g, 27 g/100 g, and 0.5 g/100 g, respectively. RSA and FRAP of raw SL were 44% and 220%, respectively. Blanching decreased TF levels significantly and FRAP sligtly, but it did not change TP, CT, CL levels, and RSA. Steaming even increased CT and CL levels (39 g/100 g and 1 g/100 g, respectively), but it decreased TP levels (13 mg GAE/100 g), RSA, and most dramatically FRAP. Roasting significantly decreased TP, CT, CL, and FRAP, and it almost abolished RSA. Inhibitory activities of SL against growth, apoptosis, invasion, migration, and adhesion of cancer cells were investigated using two human colon cancer cell lines, HT29 (colon adenocarcinoma cells) and HCT116 (colorectal carcinoma cells). Raw SL inhibited cell growth (by 76-92% in HT29 cells and by 73-87% in HCT116 cells at the concentration range of 100-500 µg/ml). Blanching, steaming, and roasting did not change the growth-inhibitory activities. All types of SL increased sub-G1 cell populations (by 2-4 times in HT29 cells and by 2-5 times in HCT116 cells at the concentration of 500 µg/ml), indicating that the growth-inhibitory activities of SL may be, at least in part, due to the induction of apoptosis. Raw SL inhibited cell invasion in both HT29 (by 27% at the concentration of 50 µg/ml) and HCT116 cells (by 14% at the concentration 100 µg/ml). Both blanching and steaming resulted in increased invasion-inhibitory activities in HCT116 cells. Raw SL inhibited cell migration in both HT29 (by 27% at the concentration of 50 µg/ml) and HCT116 cells (by 29-38% at the concentration of 100 µg/ml), and blanching, steaming, and roasting resulted in increased migration-inhibitory activities in HT29 cells. Raw SL also inhibited migration of HCT116 cells (by 29-38% at the concentration of 100 µg/ml), and roasting resulted in increased migration-inhibiotry activities. Raw SL did not inhibit cell adhesion in HT29 cells, but steaming significantly increased the inhibitory activities in HT29 cells. These results indicate that antioxidant activities and in vitro anti-cancer of SL are influenced by domestic cooking.

깨 추출물의 항산화성과 항암성

송보람 충북대학교 대학원 2013 국내석사

In this study, antioxidant activities of sesame and perilla seed extracts were compared by different seeds, different solvents, and raw or roasted seed. Antioxidant compound levels and antioxidant activities were conducted by correlation analysis. Anti-cancer activities of sesame and perilla seed extracts, α, γ, δ-tocopherol, and β-sitosterol were evaluated. Antioxidant and anti-cancer activity of seed extracts were conducted by correlation analysis. 1. Total polyphenol and total flavonoid level were higher perilla extracts than other seed extracts, were higher hot water extracts than other solvents extracts in the raw seeds, were higher hot water extracts and 70% ethanol extracts than other solvents extracts in the roasted seeds, were higher raw seed extracts than roasted seed extracts in the hot water extracts, and were higher roasted seed extracts than raw seed extracts in the 70% ethanol and methanol extracts. 2. Free-radical scavenging activity and ferric reducing antioxidant power were higher perilla extracts than other seed extracts, were higher hot water extracts and 70% ethanol extracts than other solvents extracts in the raw seeds, were higher 70% ethanol extracts than other solvents extracts in the roasted seeds, were higher raw seed extracts than roasted seed extracts in the hot water extracts, and were higher roasted seed extracts than raw seed extracts in the 70% ethanol and methanol extracts. 3. Total polyphenol and total flavonoid levels had a significant positive correlation with ferric reducing antioxidant power, respectively, in the hot water, 70% ethanol, and methanol extracts. Total polyphenol and total flavonoid levels had a significant positive correlation with radical scavenging activity, respectively, in the 70% ethanol raw and roasted seed extracts and in the methanol roasted seed extracts. So, this study suggested that 70% ethanol is best solvent on antioxidant compound levels and antioxidant activities extraction. 4. Seed extracts, tocopherol(γ, δ), and β-sitosterol effected proliferation inhibition of respectively 58.0-94.1% at 1, 2, 4 mg/ml concentrations, 58.1-97.8% at 50, 75, 100 μM concentrations, and 10.9-49.6% at 25, 50, 75, 100 μM concentrations, and effected attachment inhibition of respectively 14.3-35.0% at 1, 2, 4 mg/ml concentrations, 8.6-75.4% at 25, 50, 100 μM concentrations, and 45.6-56.0% at 25, 50, 100 μM concentrations in colon cancer cell. Seed extracts, tocopherol(γ, δ), and β- sitosterol effected proliferation inhibition of respectively 27.5-65.4% at 1, 2, 4 mg/ml concentrations, 30.9-89.4% at 50, 75, 100 μM concentrations, and 45.7-68.7% at 25, 50, 75, 100 μM concentrations, and effected attachment inhibition of respectively 24.3-34.1% at 2, 4 mg/ml concentrations, 0.9-19.0% at 50, 100 μM concentrations, and 32.8-45.9% at 25, 50, 100 μM concentrations in lung cancer cell. Seed extracts, tocopherol(γ, δ), and β-sitosterol induced apoptosis, and strong coloration and contraction of cells was observed in both colon cancer cell respectively at 1 mg/ml, 50 μM, 50 μM concentrations and lung cancer cell respectively at 2 mg/ml, 50 μM, 50 μM concentrations. 5. Seed extract 1, 2 mg/ml concentrations effected strong colony formation inhibition that does not form single colonies in colon cancer cell. Only perilla extract 1,2 mg/ml concentrantions effected 50.5-100% colony formation inhibition in lung cancer cell. γ, δ- Tocopherol 5, 10, 50 μM concentration effected 28.0-99.9% colony formation inhibition in colon cancer cell, and γ, δ-tocopherol 50 μM concentration effected strong colony formation inhibition that does not form single colonies in lung cancer cell. β-Sitosterol effected 70.6% colony formation inhibition in colon cancer cell, and effected 42.1% colony formation inhibition in lung cancer cell. α-Tocopherol not effected proliferation inhibition, attachment inhibition and not induced apoptosis in both colon and lung cancer cell. α- Tocopherol 5, 10, 50 μM concentration effected 90.6-96.8% colony formation inhibition in colon cancer cell, but, not effected colony formation inhibition in lung cancer cell. Mechanistic studies for anticancer activities are needed. This study was conducted in vitro. In vivo studies for antioxidant and anti-cancer activities are needed in future.