Thiamine결핍 유도후 쥐 전뇌의 성상교세포 Glial Fibrillary Acidic Protein(GFAP) 면역화학 활성도에 대한 변화

김승현,김희태,김주한,김명호,황세진,김명권,백태경 대한신경과학회 1999 대한신경과학회지 Vol.17 No.1

4주간의 지구성 트레이닝과 thiamine 섭취가 골격근 내 PDH활성에 미치는 영향

황혜정(HyeJungHwang),김지수(JiSooKm),장지웅(JiWoongJang),임기원(KiWonLim),정승삼(SeungSamJoung),최성근(SungKeunChoi) 한국체육학회 2016 한국체육학회지 Vol.55 No.3

본 연구는 지구성 트레이닝과 thiamine(thiamine tetrahydrofurfuryl disulfide: TTFD)의 투여가 골격근 내 글리코겐과 PDH(Pyruvate dehydrogenase), 그리고 PDH 활성에 관여하는 효소의 단백질인 PDK4(Pyruvate dehydrogenase kinase 4)와 PDP1(PDH phosphatase 1)의 발현에 어떠한 영향을 미치는지 알아보는 것을 목적으로 하였다. 6주령의 ICR 마우스를 대상으로 비운동집단(Sedentary; CON, TH), 운동집단(Exercise; EX, THEX)으로 나누어 4주간의 지구성 트레이닝과 체중kg 당 50 mg의 thiamine을 경구투여 하였다. 4주간의 지구성 트레이닝은 간과 근육 내 glycogen의 저장량에 유의한 증가가 나타났지만 thiamine 투여에 따르는 차이는 나타나지 않았다. 마찬가지로 골격근 내 PDH와 PDH 조절에 관련한 PDK4, PDP1의 단백질 발현을 측정한 결과 4주간의 지구성 트레이닝에 따르는 효과는 관찰되었지만, thiamine 투여에 집단간 유의한 효과는 나타나지 않았다. 이러한 결과는 장기간 지구성 트레이닝에 따른 골격근의 적응으로 인하여 thiamine투여에 따른 시너지 효과가 나타나지 않은 것으로 보인다. 따라서 추후 연구에서는 지구성 트레이닝의 기간을 고려한 thiamine의 섭취 타이밍 그리고 탄수화물의 복합투여에 따른 PDH와 관련 단백질의 분석이 필요할 것으로 보인다. This study aimed to analyze PDH(Pyruvate dehydrogenase) and protein expression of PDK4(Pyruvate dehydrogenase kinase 4), PDP1(PDH phosphatase 1), enzymes that are involved in the activation of PDH, in skeletal muscle and to investigate the concentration of thiamine administration in liver and muscle following 4 weeks of endurance training. Methods : 6 weeks old male ICR mice were divided into two groups: sedentary group (CON, n=10; TH, n=10), and exercise group (EX, n=10, THEX, n=10). Thiamine(thiamine tetrahydrofurfuryl disulfide: TTFD) TTFD was orally administrated into TH and THEX groups in 50mg/kg body weight for 4 weeks. Treadmill training was performed in EX and THEX groups at about 70% of VO2max for 5 times a week for 4 weeks. Results : In this study, the concentration of glycogen was significantly increased following 4 weeks of endurance training, but a significant difference was not found following thiamine administration. Similarly, there was a significant effect of the training on PDH and the expression of PDK4 and PDP1 as PDH was increased by about 40% along with the increase in PDK4 and PDP1. However, there was no significant difference found between the groups following thiamine administration. Discussion : This result shows that there was no synergistic effect of thiamine administration, potentially due to adaptation of skeletal muscle from a long-term endurance training. Therefore, it will be necessary to consider the intake timing of thiamine and to analyze proteins that are related to PDH following the administration of complex carbohydrates.

알코올 사용 장애 환자에서 동반되는 베르니케 뇌병증의 선별검사로서 티아민 혈중농도의 진단적 유용성

최동렬(Dongryeol Choi),정영철(Young-Chul Jung) 한국중독정신의학회 2017 중독정신의학 Vol.21 No.1

Objectives : The purpose of this study was to evaluate the utility in measuring the blood thiamine and thiamine pyrophosphate (TPP) levels as screening tests for Wernicke encephalopathy in patients with alcohol use disorder. Methods : We analyzed the demographic, clinical and laboratory data from 35 patients with alcohol use disorder. The blood thiamine and TPP levels were acquired at admission prior to initiation of thiamine intravenous (IV) therapy. We examined whether the blood thiamine and TPP levels correlated with clinical symptoms by apply-ing the Caine’s criteria. Patients who met at least one of Caine’s criteria were considered at high risk for Wernicke encephalopathy and were treated with high-dose thiamine IV therapy. Results : There was no correlation between blood thiamine/TPP levels and clinical symptoms of Wernicke encephalopathy. Clin-ical manifestations of the below-normal TPP-level group were not different from the normal TPP-level group, except that the normal TPP-level group showed a higher revised clinical insti-tute withdrawal assessment for alcohol (CIWA) scores. Conclusion : Measuring blood thiamine/TPP levels was not sensitive as screening tests for Wernicke encephalopathy among patients with alcohol use disorder. High-dose thiamine IV therapy improved the clinical symptoms of Wernicke encephalopathy in patients with alcohol use disorder. Objectives : The purpose of this study was to evaluate the utility in measuring the blood thiamine and thiamine pyrophosphate (TPP) levels as screening tests for Wernicke encephalopathy in patients with alcohol use disorder. Methods : We analyzed the demographic, clinical and laboratory data from 35 patients with alcohol use disorder. The blood thiamine and TPP levels were acquired at admission prior to initiation of thiamine intravenous (IV) therapy. We examined whether the blood thiamine and TPP levels correlated with clinical symptoms by apply-ing the Caine’s criteria. Patients who met at least one of Caine’s criteria were considered at high risk for Wernicke encephalopathy and were treated with high-dose thiamine IV therapy. Results : There was no correlation between blood thiamine/TPP levels and clinical symptoms of Wernicke encephalopathy. Clin-ical manifestations of the below-normal TPP-level group were not different from the normal TPP-level group, except that the normal TPP-level group showed a higher revised clinical insti-tute withdrawal assessment for alcohol (CIWA) scores. Conclusion : Measuring blood thiamine/TPP levels was not sensitive as screening tests for Wernicke encephalopathy among patients with alcohol use disorder. High-dose thiamine IV therapy improved the clinical symptoms of Wernicke encephalopathy in patients with alcohol use disorder.

Regular moderate exercise training can alter the urinary excretion of thiamin and riboflavin

Young-Nam Kim,Ji Young Choi,Youn-Ok Cho 한국영양학회 2015 Nutrition Research and Practice Vol.9 No.1

BACKGROUND/OBJECTIVES: Physical exercise promotes energy producing pathways requiring thiamin and riboflavin as a coenzyme. Therefore, this study investigated the effects of regular exercise training on urinary excretion of thiamin and riboflavin. MATERIALS/METHODS: Fifty rats were randomly assigned to one of two groups: non-exercise training (NT, n = 25) and regular exercise training (ET, n = 25) for 5 weeks. The rats performed moderate exercise on a treadmill (0.5-0.8 km/hour) for 30 min/day, 5 days/week. Twenty-four hour urine samples were collected at the end of the 0 week, 3rd week, and 5th week of training and thiamin and riboflavin were analyzed. RESULTS: No significant differences in thiamin and riboflavin intakes for each week were observed between the NT and ET groups. Urinary thiamin excretion of each group was the highest at the 5<SUP>th</SUP> week compared to the levels at 0 and 3<SUP>rd</SUP> week. Urinary thiamin at the 5<SUP>th</SUP> week was significantly lower in the ET group than in the NT group. Urinary riboflavin excretion was increased by training duration, however, no difference was observed between NT and ET for each week. At 0 and 3<SUP>rd</SUP> week, no significant relationships were observed between dietary intake and urinary excretion of thiamin and riboflavin, however, at the 5<SUP>th</SUP> week, urinary excretion was significantly increased by dietary intake only in the NT group (P < 0.05). Thiamin excretion of both NT and ET groups was significantly increased with riboflavin excretion at the 5<SUP>th</SUP> week (P < 0.01). CONCLUSION: Regular moderate exercise training increased urinary excretion of thiamin. Dietary intakes and urinary excretions of thiamin and riboflavin showed positive correlation in both the exercise training and non-exercise training groups as the exercise training period went by, while the correlations in the exercise training group were weaker than those in the non-exercise training group. Therefore, regular exercise training can alter the urinary excretion of thiamin and riboflavin in rats.

Characteristics of Thiamine Uptake by the BeWo Human Trophoblast Cell Line

Keating, Elisa,Lemos, Clara,Azevedo, Isabel,Martel, Fatima Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.4

Little is known concerning the mechanisms responsible for the transplacental transfer of thiamine. So, the aim of this work was to characterize the placental uptake of thiamine from the maternal circulation, by determining the characteristics of $^3H$-thiamine uptake by a human trophoblast cell line (BeWo). Uptake of $^3H$-thiamine (50-100 nM) by BeWo cells was: 1) temperature-dependent and energy-independent; 2) pH-dependent (uptake increased as the extracellular medium pH decreased); 3) $Na^+$-dependent and $Cl^-$-independent; 4) not inhibited by the thiamine structural analogs amprolium, oxythiamine and thiamine pyrophosphate; 5) inhibited by the unrelated organic cations guanidine, N-methylnicotinamide, tetraethylammonium, clonidine and cimetidine; 6) inhibited by the organic cation serotonin, and by two selective inhibitors of the serotonin plasmalemmal transporter (hSERT), fluoxetine and desipramine. We conclude that $^3H$-thiamine uptake by BeWo cells seems to occur through a process distinct from thiamine transporter-1 (hThTr-1) and thiamine transporter-2 (hThTr-2). Rather, it seems to involve hSERT. Moreover, chronic (48 h) exposure of cells to caffeine ($1\;{\mu}M$) stimulated and chronic exposure to xanthohumol and iso-xanthohumol (1 and $0.1\;{\mu}M$, respectively) inhibited $^3H$-thiamine uptake, these effects being not mediated through modulation of the expression levels of either hThTr-1 or hSERT mRNA.

국내 식품 중 구이, 찜, 볶음, 조림에 존재하는 수용성 비타민 B₁, B₂ 그리고 B₃ 함량 조사

조진주,홍성준,부창국,정유리,정창현,신의철,Cho, Jin-Ju,Hong, Seong Jun,Boo, Chang Guk,Jeong, Yuri,Jeong, Chang Hyun,Shin, Eui-Cheol 한국식품위생안전성학회 2019 한국식품위생안전성학회지 Vol.34 No.5

A conventional Korean meal typically includes various roasted, steamed, stir-fried, and braised foods. For this study, we investigated the contents of water soluble vitamins, $B_1$ (thiamin), $B_2$ (riboflavin) and $B_3$ (niacin) in various roasted, steamed, stir-fried, and braised foods. Method validation for analytical data in this study showed a high linearity ($r^2$>0.999), and the limit of detection and quantification were 0.001-0.067 and $0.002-0.203{\mu}g/mL$, respectively. For accuracy and precision, analytical values using standard reference materials were in the certified ranges. Roasted foods contained 0.039-1.057 mg/100 g of thiamin, 0.058-0.686 mg/100 g of riboflavin and 0.021-21.772 mg/100 g of niacin. Steamed foods contained 0.049-1.066 mg/100 g of thiamin, 0.025-0.548 mg/100 g of riboflavin and 0.134-21.509 mg/100 g of niacin. Stir-fried foods contained 0.114-0.388 mg/100 g of thiamin, 0.014-1.258 mg/100 g of riboflavin and 0.015-2.319 mg/100 g of niacin. Braised foods contained 0.112-1.656 mg/100 g of thiamin, 0.024-0.298 mg/100 g of riboflavin and 0.322-2.157 mg/100 g of niacin. The data on water-soluble vitamins in this study can be used for a nutritional database of conventional Korean meals. 본 연구는 국내 식생활의 기본적인 구성식단으로 알려진 구이, 찜, 볶음, 조림에 존재하는 다양한 영양성분 중 수용성 비타민인 $B_1$ (thiamin), $B_2$ (riboflavin), 그리고 $B_3$ (niacin)의 함량을 확인하였다. 실험분석 타당성 검증을 통해서 높은 직선성($r^2$>0.997)을 확인하였고, 검출한계의 경우 $0.001-0.067{\mu}g/mL$ 그리고 정량한계의 경우 $0.002-0.203{\mu}g/mL$를 확인하였다. 또한 실험값에 대한 정밀도와 반복성에 대한 검증을 위해서 standard reference materials를 통해 실험값과 표준값과의 오차가 신뢰도 이내에 존재한다는 결과를 확인하였다. 본 연구에서 제시된 구이식품의 경우 thiamin은 0.039-1.057 mg/100 g의 범위에 존재하였고, riboflavin은 0.058-0.686 mg/100 g의 범위에 존재했으며, niacin의 경우 0.021-21.772 mg/100 g 의 범위로 나타났다. 찜 종류의 경우 thiamin은 0.049-1.066 mg/100 g의 범위에 존재하였고, riboflavin은 0.025-0.548 mg/100 g의 범위에 존재했으며, niacin의 경우 0.134-21.509 mg/100 g의 범위로 나타났다. 볶음의 경우 thiamin은 0.114-0.388 mg/100 g의 범위에 존재하였고, riboflavin은 0.014-1.258 mg/100 g의 범위에 존재했으며, niacin의 경우 0.015-2.319 mg/100 g의 범위로 나타났다. 조림의 경우 thiamin은 0.112-1.656 mg/100 g의 범위에 존재하였고, riboflavin은 0.024-0.298 mg/100 g의 범위에 존재했으며, niacin의 경우 0.322-2.157 mg/100 g의 범위로 나타났다. 본 연구에서 제시된 구이, 찜, 볶음, 그리고 조림 식품에 대한 수용성 비타민 함량에 대한 영양성분 데이터베이스 구축 연구가 국민 식생활의 중요한 기초자료로 이용될 것으로 확신한다.

Effects of Thiamine Pyrophosphate on the Inhibition of Self-splicing of Primary Transcripts of T4 phage Thymidylate Synthase Gene in the Presence of GTP

HyunJooLee,SungJoonAhn,SookShin,InKookPark 한국미생물학회 2002 The journal of microbiology Vol.40 No.2

Effects of GTP on the inhibition of self-splicing of primary transcripts of the phage T4 thymidylate synthase gene (td) by thiamine pyrophosphate and its analogs have been investigated. The order of the inhibitory efficiency for compounds tested was as follows: thiamine pyrophosphate > thiamine monophosphate > thiamine. Of all compounds examined, thiamine pyrophosphate was the most potent inhibitor. Increasing GTP concentration in splicing reaction tended to overcome the suppressive effects of self-splicing by thiamine pyrophosphate and its analogs. The inhibition by thiamine pyrophosphate was most sensitized to a higher concentration of GTP. It has been speculated that the key structural features in thiamine pyrophosphate and its analogs responsible for the inhibition of splicing may be a thiamine moiety in which the phosphorylation of 2-hydroxylethyl group on 5-position of thiazolium ring rendered further stimulation of inhibition in self-splicing reaction..

차나무 잎의 캘러스 배양을 통한 카테킨류의 생산성 개선

오순자(Soonja Oh),고석찬(Seok Chan Koh) 한국자원식물학회 2005 한국자원식물학회지 Vol.18 No.2

차나무 카테킨류의 생산성을 높이기 위하여 차나무 잎의 캘러스 배양시 thiamine-HCl과PVP의 효과를 조사하였다. 캘러스의 생장은 20 mg/L thiamine-HCl이 포함된 캘러스증식배지(0.2 mg/L 2,4-D와 1.0 mg/L TDZ가 첨가된 MS 배지)에서 가장 왕성하여 대조구에 비해 1.7배의 생장을 보였다. 배양된 캘러스의 카페인 함량은 신초에 비해 10% 이하로 낮아졌고 총 카테킨류 함량은 40 mg/L thiamine-HCl에서 가장 높아 신초의 1.6배까지 증가하였다. 특히 신초에서는 검출되지 않았던 (-)-epicatechin(EC)이 thiamine-HCl 또는 PVP를 처리하여 배양하였을 경우 모든 처리에서 검출되었으며, (-)-epicatechingallate(ECG)는 신초에서 보다 4.8배나 높은 함량을 나타내었다. 캘러스 생장과 고효율의 카테킨 생산을 고려했을 때 30 mg/L thiamine-HCl이 포함된 배지가 최적의 배양조건으로 판단된다. The effects of thiamine-HCl or polyvinylpyrrolidone (PVP) on growth and catechin contents of calli from Camellia sinensis leaves were investigated to improve catechin productivity in callus cultures on the selective medium. The growth of calli was great on the proliferation medium (the MS medium with 0.2 mg/L 2,4-D and 1.0 mg/L TDZ) supplemented with 20~30mg/L thiamine-HCl. Although the caffeine content was low in calli compared to young leaves, the total content of catechins was high in calli. Particularly,(-)-epicatechin (EC) which not detected in young loaves was also detected in calli on the propagation medium containing thiamine-HCl or PVP. In addition, the (-)-epicatechingallate (ECG) content was significantly higher in calli than in young leaves. In conclusion, the proliferation medium supplemented with 30 mg/L thiamine-HCl as a vitamin source seemed to be optimal condition for the growth and catechin production in callus culture.

Cardiovascular beriberi: rare cause of reversible pulmonary hypertension

Song, Joon Hyuk,Cheon, Sang Soo,Bae, Myung Hwan,Lee, Jang Hoon,Yang, Dong Heon,Park, Hun Sik,Cho, Yongkeun,Chae, Shung Chull Yeungnam University College of Medicine 2014 Yeungnam University Journal of Medicine Vol.31 No.1

Cardiovascular beriberi is caused by thiamine deficiency and usually presents as high cardiac output failure associated with predominantly right-sided heart failure and rapid recovery after treatment with thiamine. Because of its rarity in developed countries, the diagnosis can often be delayed and missed. We recently experienced a case of cardiovascular beriberi with pulmonary hypertension which successfully treated with thiamine infusion. A 50-year-old man with chronic heavy alcoholics was refered to our department for dyspnea with mental change. Echocardiography showed marked right ventricular (RV) dilatation and flattening of the interventricular septum with a D-shaped deformation of the left ventricle. Moderate tricuspid valve regurgitation was found and estimated RV systolic pressure was 52 mm Hg. Because of his confused mentality and history of chronic alcohol intake, neurological disorder due to thiamine deficiency was suspected and intravenous thiamine was administered and he continuously received a daily dose of 100 mg of thiamine. Follow up echocardiography showed marked reduction of RV dilatation and improvement of a D-shaped deformation of the left ventricle. He finally diagnosed as cardiovascular beriberi on the basis of dramatic response to intravenous thiamine. Thiamine deficiency can cause reversible pulmonary hypertension, and can still be encountered in the clinical setting. Thus high index of suspicion is critically needed for diagnosis.

Cardiovascular beriberi: rare cause of reversible pulmonary hypertension

송준혁,전상수,배명환,이장훈,양동헌,박훈식,조용근,채성철 영남대학교 의과대학 2014 Yeungnam University Journal of Medicine Vol.31 No.1

Cardiovascular beriberi is caused by thiamine deficiency and usually presents as high cardiac output failure associated with predominantly right-sided heart failure and rapid recovery after treatment with thiamine. Because of its rarity in developed countries, the diagnosis can often be delayed and missed. We recently experienced a case of cardiovascular beriberi with pulmonary hypertension which successfully treated with thiamine infusion. A 50-year-old man with chronic heavy alcoholics was refered to our department for dyspnea with mental change. Echocardiography showed marked right ventricular (RV) dilatation and flattening of the interventricular septum with a D-shaped deformation of the left ventricle. Moderate tricuspid valve regurgitation was found and estimated RV systolic pressure was 52 mm Hg. Because of his confused mentality and history of chronic alcohol intake, neurological disorder due to thiamine deficiency was suspected and intravenous thiamine was administered and he continuously received a daily dose of 100 mg of thiamine. Follow up echocardiography showed marked reduction of RV dilatation and improvement of a D-shaped deformation of the left ventricle. He finally diagnosed as cardiovascular beriberi on the basis of dramatic response to intravenous thiamine. Thiamine deficiency can cause reversible pulmonary hypertension, and can still be encountered in the clinical setting. Thus high index of suspicion is critically needed for diagnosis.