Anti-osteoclastic effect of caffeic acid phenethyl ester in murine macrophages depends upon the suppression of superoxide anion production through the prevention of an active-Nox1 complex formation

Kwon, Yong-Beom,Wang, Fang-Fang,Jang, Hae-Dong Elsevier 2018 The Journal of nutritional biochemistry Vol.58 No.-

<P><B>Abstract</B></P> <P>This study investigated the anti-osteoclastic effect of caffeic acid phenethyl ester (CAPE) through suppression of Nox1-mediated superoxide anions production. The multi-nucleated cells were counted and followed by measuring their tartrate-resistant acid phosphatase (TRAP) activity. The superoxide anion production was determined by using fluorescent probe dihydroethidium (DHE). After one day of exposure to the receptor activator of nuclear factor-κB ligand (RANKL), the expression of the proteins involved in superoxide anion production was determined by western blotting. A potent anti-osteoclastic effect of CAPE was observed; the superoxide anion level reached a maximum value after one day of incubation. CAPE attenuated the expression of NADPH (nicotinamide adenine dinucleotide phosphate) oxidase 1 (Nox1) and Rac1, and mitigated the RANKL-induced translocation of p47<SUP>phox</SUP> to the cell membrane. In addition, CAPE suppressed the expression of nuclear factor-kappa B (NF-κB p65), its translocation to the nucleus, and the activation of NF-κB inhibitor (IκBα) and its kinase (IKKβ). Furthermore, CAPE diminished the expression and activation of the c-jun N-terminal kinase (JNK) and the expression of protein-1 activators (AP-1) such as c-Fos and c-Jun. The expression of Nox1 was suppressed by CAPE through the down-regulation of IKKβ/IκBα/NF-κB and JNK/AP-1 signal pathway. This study provides evidence that the anti-osteoclastic effect of CAPE depends upon the attenuated superoxide anion production, which is closely related with interruption of an active Nox1 complex formation due to the attenuated catalytic subunit Nox1 expression resulting from suppression of the IKKβ/IκBα/NF-κB and JNK/AP-1 signaling pathway and the down-regulation of the p47<SUP>phox</SUP> subunit translocation to the cell membrane.</P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>

Alterations of O₂Metabolite Scavengers and Production of Superoxide Redicals in Ischemic Rat Kidney

Kim, Myoung-Choel,Lee, Byoung-Rai 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.2

30분동안 허혈상태를 유지한 백서의 신장에서 O_(2) metabolite scavengers의 변화와 허혈상태 후 재관류시에 superoxide radicals의 생성기전에 관하여 실험하였다. 허혈상태에서 superoxide dismutase, glutathione peroxidase및 catalase는 대조군에 비해서 각각 18%(P<0.01), 8%, 22%(P<0.01)씩 감소하였다. 그리고 Reduced glutathiones(GSH) 및 ATP는 각각 30%(P<0.05), 60%(P<0.01)씩 감소하였다. 일반적으로 허혈 후 재관류시 superoxide radicals 의 주요 생성기정으로 알려진 xanthine dehydrogenase의 xanthine oxidase로의 전환은 xanthine oxidase/(xanthine dehydrogenase+xanthine oxidase)로 나타낼때, 허혈신장에서는 대조군에 비해 12%(P<0.01)증가 하였다. 또한 xanthine oxidase inhibitor로 알려진 allopurinol(50㎎/Kg)을 전처치한 후 허혈 및 재관류시의 손상을 실험한 결과,calcium antagonist인 verapamil(10㎎/Kg)의 경우보다 손상에 대한 방어효과가 더 적었다. Submitochondrial particles를 함유한 reaction mixture에서 superoxide radicals의 생성은 가해준 calcium(300-900uM)의 농도에 비례해서 증가함을 보였다. 실험적으로 증가된 NADH농도는 submiochondrial particles에서 superoxide radicals의 생성을 증가시켰으며, 실제로 허혈상태의 신장에서 NADH농도가 증가하였고(30.42%(P<0.05)) 허혈 후 재관류시는 감소하였다(13%(P<0.01)). 이상의 실험결과로서 허혈시 O_(2) metabolite scavengers는 그기능이 감소되며, 허혈후 재관류시 xanthine oxidase system이외에 NADH의 증가와 calcium의 증가가 superoxide radicals의 생성에 관여함을 알았다.

Korean Red Ginseng Extract inhibits Tumor Necrosis Factor-alpha-induced Monocyte Adhesion in the Human Endothelial Cells

Hee Kyoung Joo,Sang Ki Lee,Hyo Shin Kim,Yun Jeong Song,Gun Kang,Jin Bong Park,Kwon Ho Lee,Eun Jung Cho,Jae-Hwan Lee,In-Whan Seong,Se Hoon Kim,Chung-Hyun Cho,Byeong Hwa Jeon 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.3

Vascular inflammation is an important step in the development of cardiovascular disorder. Since it has not been known whether Korean red ginseng has a role to play on the vascular inflammation, we investigated the effects of Korean red ginseng extract (KRGE) on monocyte adhesion and its underlying signaling mechanism. Monocyte adhesion assay and Western blot were conducted on the human umbilical vein endothelial cells to study monocyte adhesion and the expression of adhesion molecules. Intracellular calcium was measured with Fura-2 fluorescent staining, and superoxide production was measured with lucigenin chemiluminescence in the endothelial cells. KRGE inhibits tumor necrosis factor (TNF)-alpha-induced monocyte adhesion on the endothelial cells at the range of 0.03~1 ㎎/㎖. TNF-alpha-induced vascular cell adhesion molecule-1 and intercellular cell adhesion molecule-1 expression were inhibited by the pretreatment of KRGE in the endothelial cells. KRGE also inhibits TNF-alpha-induced intracellular calcium and the superoxide production in the endothelial cells. This study first demonstrated that KRGE inhibits TNF-alpha-induced monocyte adhesion by inhibiting the adhesion molecule expression, intracellular calcium and superoxide production in the endothelial cells. Therefore, the anti-inflammatory function of KRGE may be contributed to protecting the endothelial dysfunction in the vascular inflammatory disorders.

Korean Red Ginseng Extract inhibits Tumor Necrosis Factor-alpha-induced Monocyte Adhesion in the Human Endothelial Cells

Joo, Hee-Kyoung,Lee, Sang-Ki,Kim, Hyo-Shin,Song, Yun-Jeong,Kang, Gun,Park, Jin-Bong,Lee, Kwon-Ho,Cho, Eun-Jung,Lee, Jae-Hwan,Seong, In-Whan,Kim, Se-Hoon,Cho, Chung-Hyun,Jeon, Byeong-Hwa The Korean Society of Ginseng 2008 Journal of Ginseng Research Vol.32 No.3

Vascular inflammation is an important step in the development of cardiovascular disorder. Since it has not been known whether Korean red ginseng has a role to play on the vascular inflammation, we investigated the effects of Korean red ginseng extract (KRGE) on monocyte adhesion and its underlying signaling mechanism. Monocyte adhesion assay and Western blot were conducted on the human umbilical vein endothelial cells to study monocyte adhesion and the expression of adhesion molecules. Intracellular calcium was measured with Fura-2 fluorescent staining, and superoxide production was measured with lucigenin chemiluminescence in the endothelial cells. KRGE inhibits tumor necrosis factor (TNF)-alpha-induced monocyte adhesion on the endothelial cells at the range of $0.03{\sim}1$ mg/ml. TNF-alpha-induced vascular cell adhesion molecule-1 and intercellular cell adhesion molecule-1 expression were inhibited by the pretreatment of KRGE in the endothelial cells. KRGE also inhibits TNF-alpha-induced intracellular calcium and the superoxide production in the endothelial cells. This study first demonstrated that KRGE inhibits TNF-alpha-induced monocyte adhesion by inhibiting the adhesion molecule expression, intracellular calcium and superoxide production in the endothelial cells. Therefore, the anti-inflammatory function of KRGE may be contributed to protecting the endothelial dysfunction in the vascular inflammatory disorders.

Korean Red Ginseng Extract inhibits Tumor Necrosis Factor-alpha-induced Monocyte Adhesion in the Human Endothelial Cells

주희경,이상기,김효신,송윤정,강건,박진봉,이권호,조은정,이재환,성인환,김세훈,조충현,전병화 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.3

Vascular inflammation is an important step in the development of cardiovascular disorder. Since it has not been known whether Korean red ginseng has a role to play on the vascular inflammation, we investigated the effects of Korean red ginseng extract (KRGE) on monocyte adhesion and its underlying signaling mechanism. Monocyte adhesion assay and Western blot were conducted on the human umbilical vein endothelial cells to study monocyte adhesion and the expression of adhesion molecules. Intracellular calcium was measured with Fura-2 fluorescent staining, and superoxide production was measured with lucigenin chemiluminescence in the endothelial cells. KRGE inhibits tumor necrosis factor (TNF)-alpha-induced monocyte adhesion on the endothelial cells at the range of 0.03~1 mg/ml. TNF-alpha-induced vascular cell adhesion molecule-1 and intercellular cell adhesion molecule-1 expression were inhibited by the pretreatment of KRGE in the endothelial cells. KRGE also inhibits TNF-alpha-induced intracellular calcium and the superoxide production in the endothelial cells. This study first demonstrated that KRGE inhibits TNF-alpha-induced monocyte adhesion by inhibiting the adhesion molecule expression, intracellular calcium and superoxide production in the endothelial cells. Therefore, the anti-inflammatory function of KRGE may be contributed to protecting the endothelial dysfunction in the vascular inflammatory disorders.

Anaerobic Respiration of Superoxide Dismutase-Deficiant Saccharomyces cerevisiae under Oxidative Stress

Lee, Sun Mi,Nam, Doo Hyun 영남대학교 약품개발연구소 1998 영남대학교 약품개발연구소 연구업적집 Vol.8 No.-

The ethanol productivity of superoxide dismutase (SOD)-deficient mutants of Saccharomyces cerevisiae was examined under the oxidative stress by Paraquat. It was observed that MnSOD-deficient mutant of S. cerevisiae had higher ethanol productivity than wild type or CuZnSOD-deficient yeast both in aerobic and in anaerobic culture condition. Pyruvate dehydrogenase activity decreased by 35% and alcohol dehydrogenase activity increased by 32% were observed in MnSOD-deficient yeast grown aerobically. When generating oxygen radicals by Paraquat, the ethanol productivity was increased by 40% in CuZnSOD-deficient or wild strain, resulting from increased activity of alcohol dehydrogenase and decreased activity of pyruvate dehydrogenase. However, the addition of ascorbic acid with Paraquat returned the enzyme activities at the level of control. These results imply that SOD-deficiency in yeast strains may cause the metabolic flux to shift into anaerobic ethanol fermentation in order to avoid their oxidative damages by Paraquat.

Anaerobic Respiration of Superoxide Dismutase-Deficient Saccharomyces cerevisiae under Oxidative Stress

Lee, Sun-Mi,Nam, Doo-Hyun The Korean Society for Biotechnology and Bioengine 1998 Biotechnology and Bioprocess Engineering Vol.3 No.1

The entanol productivity of superoxide dismutase (SOD)-deficient mutants of Saccharo-Myces cerevisiae was examined under the oxidative stress by Paraquat. It was observed that MnSOD-deficient mutant of S. cerevisiae had higher ethanol productivity than wild type or CuZnSOD-deficient yeast both in aerobic and in anaerobic culture condition. Pyruvated dehydrogenase activity decreased by 35% and alcohol dehydrogenase activity increased by 32% were observed in MnSOD-deficient yeast grown aerobically. When generating oxygen radicals by Paraquat, the ehanol productivity was increased by 40% in CuZnSOD-deficient or wild strain, resulting from increased activity of alcohol dehydrogenase and decreased a activity of pyruvate dehydrogenase. However, the addition of ascorbic acid with Paraquat returned the enzyme activities at the level of control. These results imply that SOD-deficiency in yeast strains may cause the metabolic flux to shift into anaerobic ethanol fermentation in order to avoid their oxidative damages by Paraquat.

불가사리(Asterias amurensis) 추출물을 첨가한 사료의 급이가 조피볼락 (Sebastes schlegeli)의 성장, 혈액성상 및 식세포 활성산소 생산에 미치는 효과

박희연 ( Hee Yeon Park ),임치원 ( Chi Won Lim ),김연계 ( Yeon Kye Kim ),최태진 ( Tae Jin Choi ),윤호동 ( Ho Dong Yoon ),이가정 ( Ka Jung Lee ),서연경 ( Yeon Kyung Seo ),김지영 ( Ji Yeong Kim ),박기의 ( Ki Eui Park ) 한국응용생명화학회 2007 Applied Biological Chemistry (Appl Biol Chem) Vol.50 No.4

UVB 조사가 생쥐 복강 대식 세포의 수와 면역학적 기능에 미치는 영향

김상태(Sang Tae Kim),김광(Kwang Kim),서기석(Kee Suck Suh) 대한피부과학회 1993 大韓皮膚科學會誌 Vol.31 No.1

Ultraviolet radiation of skin leads to a systemic alteratior tkat inhibits the normal pattern of immunologic tumor rejection., suppresses the contact hypersemisivity and transiently alters the morphology and the surface marker characteristics of Langerhans cells. Moreover, Ultraviolet radiation elaborates the ETAF, neuropeptides, proteins, and urocaicacid which may alter immunologic responses. But no other study about the effects of UVB irradiation on the systemic immunologic functions of the macrophages of internal organs was reported. The macrophages of the reticuloendothelial system (RES) play a central role in cell-mediated immunity, because they are involved both in the initiation of responses as antigen-presenting, cells, and in the effector phase as inflammatory, tumoricidal and microbicidal cells. The present study was intended to investigate the effects of UVB irradiation on the immunologic functions of mouse peritoneal macrophages. Normal 6-8-week-old BALB/c mice were exposed at the dose rate of 20mJ/cm and 40mJ/cm of UVB per day, 5 days per weeks for 2 weeks, 4 weeks and 8 weeks. Then the peritoneal macrophages were obtained from the mice and the changes of cell count, chemotactic index, phagocytic index, NBT reduction rate and superoxide (0) production were examined. The results were as follows : 1)the number of mouse peritoneal macrophages was decreased by UVB radiation, 2) the chemotactic index of mouse peritoneal macrophage was not altered by UVB radiation, 3) phagocytic activity of mouse pertoneal macrophage was significsntly decreased by UBV radiation, 4) NBT reduction rate in mouse aeritoneal macrophage after UVB radiation was sinificanily decreased in all experimental group, and 5) Superoxide (0) production in mouse peritoneal macrophage after UVB radiation was decreased in all experimental groups. (Kor J Dermstol 1993; 31 (1): 28-37)

Dissolution and ionization of sodium superoxide (NaO2) in sodium-oxygen batteries

박혁준,김진수,이병주,성원모,임희대,강기석 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0

We demonstrate the interplay of the diverse reactions in Na-O2 batteries involving a series of electrochemical and chemical reactions as a function of time. By systematically controlling the operating conditions, we observe that the galvanostatic charge/discharge profiles are sensitively affected by the conditions and durations of electrochemical operations. It is revealed that the electrochemically formed NaO2 is unstable and degrades into Na2O2·2H2O in the absence of the applied current. The spontaneous dissolution and ionization of NaO2 liberates the free O2- in the electrolyte and promotes side reactions involving the formation of Na2O2·2H2O. Based on these observations, we proposed reaction mechanisms of Na-O2 batteries under various operating conditions. This report is the first to reveal the relationships among the different discharge products observed in Na-O2 batteries.