Lycopene Intake by Canadian Women Is Variable, Similar Among Different Ages, But Greater Than That Reported for Women in Other Countries

Mackinnon, E.S.,Rao, A.V.,Rao, L.G. The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.4

Lycopene is an antioxidant associated with a reduced risk of chronic diseases common in women such as osteoporosis and cancer; however, no official recommendation for lycopene consumption exists, and intake data from Canadian women are limited. This study was designed to generate information about average lycopene intake in Canadian women of different ages. A cross-sectional study was conducted at St. Michael's Hospital in Toronto, Ontario, Canada. One hundred one women, between the ages of 25 and 70 years, who were not on any medications, were recruited to record their diet for 7 days. Statistical analyses were performed to compare the types of lycopene-containing foods consumed, associations between intake of lycopene and macro/micronutrients, and whether participant characteristics, such as body mass index, could predict lycopene intake. Average lycopene intake was $6.14{\pm}5.35\;mg/day$, which is higher than reported in other countries. Intake was similar among age groups but was highly variable. Raw tomatoes were the most frequently consumed source of lycopene, while participants with the highest lycopene intake consumed more cooked/processed tomato products than those with lower intake (P < .005). Participants 25.49 years old consumed more dried/powdered tomatoes (P < .05), pizza (P < .002), and ketchup (P < .10) than 50.70 year olds. Lycopene intake could not be predicted by any participant characteristics. In older participants, lycopene intake was positively correlated with intake of calcium, niacin, and vitamins A, D, and K ($P\;{\leq}\;.05$). These findings are significant to women's health and may contribute to the establishment of nutritional and health recommendations regarding consumption of lycopene by Canadian women to prevent chronic diseases.

Lycopene Intake by Canadian Women Is Variable, Similar Among Different Ages, But Greater Than That Reported for Women in Other Countries

E.S. Mackinnon,A.V. Rao,L.G. Rao 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.4

Lycopene is an antioxidant associated with a reduced risk of chronic diseases common in women such as osteoporosis and cancer; however, no official recommendation for lycopene consumption exists, and intake data from Canadian women are limited. This study was designed to generate information about average lycopene intake in Canadian women of different ages. A cross-sectional study was conducted at St. Michael's Hospital in Toronto, Ontario, Canada. One hundred one women, between the ages of 25 and 70 years, who were not on any medications, were recruited to record their diet for 7 days. Statistical analyses were performed to compare the types of lycopene-containing foods consumed, associations between intake of lycopene and macro/micronutrients, and whether participant characteristics, such as body mass index, could predict lycopene intake. Average lycopene intake was 6.14±5.35mg/day, which is higher than reported in other countries. Intake was similar among age groups but was highly variable. Raw tomatoes were the most frequently consumed source of lycopene, while participants with the highest lycopene intake consumed more cooked/processed tomato products than those with lower intake (P<.005). Participants 25–49 years old consumed more dried/powdered tomatoes (P<.05), pizza (P<.002), and ketchup (P<.10) than 50–70 year olds. Lycopene intake could not be predicted by any participant characteristics. In older participants, lycopene intake was positively correlated with intake of calcium, niacin, and vitamins A, D, and K (P≤.05). These findings are significant to women's health and may contribute to the establishment of nutritional and health recommendations regarding consumption of lycopene by Canadian women to prevent chronic diseases.

Lycopene의 새로운 혈관내피세포 생리활성

Jingu Cho(조진구),Sunghyen Kim(김성현),Jeonghwa Seo(서정화),Sunyoung Ahn(안선영),Eunsil Jeong(정은실),Heonyong Park(박헌용) 한국생명과학회 2010 생명과학회지 Vol.20 No.7

항암 효과와 항산화 기능을 가지는 것으로 알려진 lycopene은 심혈관에서의 기능이 현재까지 잘 알려져 있지 않다. 본 연구에서는 lycopene의 심혈관 기능을 알기 위해 혈관내피세포를 이용해 다양한 세포실험을 수행하였다. 그 결과, lycopene은 내피세포의 성장 및 이동을 촉진하였으나 세포사멸에는 영향이 없었다. 또한, 백혈구의 혈관내피세포 부착을 억제하였고 내피세포 내 신호전달물질인 MAPK들의 활성을 촉발하였다. MAPK의 활성 억제제를 이용한 신호전달기전 연구실험 결과, ERK와 p38 MAPK의 활성은 세포성장에 관여하고, JNK의 활성은 세포이동에 관여함을 확인하였다. 종합하면, lycopene은 혈관내피세포의 신호전달 물질인 MAPK들를 통해 세포 성장 및 이동을 촉진시키며, LPS에 의한 THP-1의 내피세포 부착을 억제 하는 등 혈관내피세포의 다양한 생리활성을 조절한다. Lycopene의 이러한 혈관내피세포 기능들은 lycopene이 혈관질환 치료제로 응용될 가능성이 있음을 의미한다. Little is known about the cardiovascular effects of Lycopene, an anti-cancer and anti-oxidative agent. In this study, we executed a series of experiments with vascular endothelial cells to disclose the cardiovascular functions of lycopene. From our in vitro experiments, lycopene was determined to act as a stimulant to induce endothelial cell proliferation and migration. In addition, lycopene was shown to inhibit lipopolysaccharide (LPS)-induced adhesion of THP-1 leukocytes to endothelial cells, as well as activating mitogen activated protein kinase (MAPK) family members, ERK, JNK and p38 MAPK. Both ERK and p38 MAPK were involved in lycopene-induced cell proliferation, while JNK was involved in lycopene-dependent cell migration. Taken together, lycopene activates MAPK family members which regulate cell proliferation and migration. Lycopene differentially blocks LPS-dependent adhesion for THP-1 to endothelial cells, indicating that lycopene is likely to regulate a variety of vascular functions.

β-Carotene과 Lycopene의 양자역학 및 화학적 항산화능과 세포 내 산화적 스트레스 보호 효과의 비교

박선영(Sun Young Park),정하나(Hana Jung),진창호(Changho Jhin),황금택(Keum Taek Hwang),곽호경(Ho-Kyung Kwak) 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.9

본 연구는 β-carotene과 lycopene의 양자역학 및 화학적 항산화능과 세포 내 항산화 효과를 분석하였다. β-Carotene과 lycopene의 항산화능을 양자역학적으로 비교한 결과 lycopene의 항산화능이 β-carotene보다 높은 것으로 나타났으며, 이러한 항산화능의 차이는 FRAP로 측정하였을 때도 유사하게 관찰되었다. Trolox와 비교했을 때 β-carotene과 lycopene의 FRAP 수치는 유의적으로 낮았으나 LPS에 의한 세포 내 ROS 생성을 낮추는 데는 두 카로티노이드가 더 우수한 것으로 나타났다. 그러므로 본 연구에서 비교한 β-carotene과 lycopene의 양자역학적 항산화능은 화학적 항산화능을 일부 반영하지만, 실제 세포 내 ROS에 의한 산화스트레스 저하 효과와는 차이가 있는 것으로 나타났다. The aim of this study was to determine the chemical and intracellular antioxidant activities of β-carotene and lycopene and to compare their quantitative structure-activity relationship (QSAR). In our previous study, the second ionization energy of lycopene was higher than that of β-carotene, as calculated by QSAR. Chemical antioxidant activities of β-carotene, lycopene, and Trolox were examined by measuring ferric reducing antioxidant power (FRAP) and 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Intracellular antioxidant activities were evaluated by intracellular reactive oxygen species (ROS) and DNA fragmentation. The FRAP of lycopene was higher than that of β-carotene (P<0.05), and the two carotenoids had similar antioxidant activities in DPPH radical scavenging activity assay. Trolox had the greatest chemical antioxidant activities (P<0.05). When RAW264.7 cells were treated with lipopolysaccharide (LPS) (100 ng/mL) for 20 h, intracellular ROS and DNA fragmentation significantly increased (P<0.05). RAW 264.7 cells pretreated with β-carotene (4 μM) and lycopene (0.4∼2 μM) for 4 h formed significantly less intracellular ROS than LPS-treated control cells (P<0.05), whereas cells with Trolox did not reduce production of intracellular ROS. In addition, cells pretreated with 2 μM lycopene produced less intracellular ROS than those treated with β-carotene (P<0.05). DNA fragmentation of cells with β-carotene and lycopene was similar to that of LPS-treated control cells as measured by Hoechst staining. The antioxidant ability of lycopene was greater than that of β-carotene in the QSAR, FRAP, and intracellular ROS assays (P<0.05). β-Carotene and lycopene had lower antioxidant activities as measured by FRAP (P<0.05) but higher intracellular protective effects against LPS-induced oxidative stress in comparison with Trolox.

Lycopene Protects Against Spontaneous Ovarian Cancer Formation in Laying Hens

Kazim Sahin,Engin Yenice,Mehmet Tuzcu,Cemal Orhan,Cengizhan Mizrak,Ibrahim H. Ozercan,Nurhan Sahin,Bahiddin Yilmaz,Birdal Bilir,Bulent Ozpolat,Omer Kucuk 대한암예방학회 2018 Journal of cancer prevention Vol.23 No.1

Background: Dietary intake of lycopene has been associated with a reduced risk of ovarian cancer, suggesting its chemopreventive potential against ovarian carcinogenesis. Lycopene’s molecular mechanisms of action in ovarian cancer have not been fully understood. Therefore, in the present study, we investigated the effects of lycopene on the ovarian cancer formation using the laying hen model, a biologically relevant animal model of spontaneous ovarian carcinogenesis due to high incidence rates similar to humans. Methods: In this study, a total of 150 laying hens at age of 102 weeks were randomized into groups of 50: a control group (0 mg of lycopene per kg of diet) and two treatment groups (200 mg or 400 mg of lycopene per kg of diet, or ~26 and 52 mg/d/hen, respectively). At the end of 12 months, blood, ovarian tissues and tumors were collected. Results: We observed that lycopene supplementation significantly reduced the overall ovarian tumor incidence (P < 0.01) as well as the number and the size of the tumors (P < 0.004 and P < 0.005, respectively). Lycopene also significantly decreased the rate of adenocarcinoma, including serous and mucinous subtypes (P < 0.006). Moreover, we also found that the serum level of oxidative stress marker malondialdehyde was significantly lower in lycopene-fed hens compared to control birds (P < 0.001). Molecular analysis of the ovarian tumors revealed that lycopene reduced the expression of NF-B while increasing the expression of nuclear factor erythroid 2 and its major target protein, heme oxygenase 1. In addition, lycopene supplementation decreased the expression of STAT3 by inducing the protein inhibitor of activated STAT3 expression in the ovarian tissues. Conclusions: Taken together, our findings strongly support the potential of lycopene in the chemoprevention of ovarian cancer through antioxidant and anti-inflammatory mechanisms. (J Cancer Prev 2018;23:25-36)

Identification of genes affecting lycopene accumulation in Escherichia coli using a shot-gun method

Kang, Min Jung,Lee, Young Mi,Yoon, Sang Hwal,Kim, Jung Heon,Ock, So Won,Jung, Kyung Hwa,Shin, Yong Chul,Keasling, Jay D.,Kim, Seon Won Wiley Subscription Services, Inc., A Wiley Company 2005 Biotechnology and bioengineering Vol.91 No.5

<P>Genes enhancing lycopene production in Escherichia coli were identified through colorimetric screening of shot-gun library clones constructed with E. coli chromosomal DNA. These E. coli cells had been engineered to produce lycopene, a red-colored carotenoid, which enabled screening for genes that enhance lycopene production. Six clones with enhanced lycopene production were isolated. Among 13 genes in these clones, dxs, appY, crl, and rpoS were found to be involved in enhanced lycopene production. While dxs and rpoS have been already reported to enhance lycopene production, appY and crl have not. DXP (1-deoxy-D-xylulose-5-phosphate) synthase is encoded by dxs and participates in the rate-limiting step in the synthesis of isopentenyl pyrophosphate (IPP), a building block of lycopene. Sigma S factor, encoded by rpoS, regulates transcription of genes induced at the stationary phase. The appY and crl genes encode transcriptional regulators related to anaerobic energy metabolism and the formation of curli surface fibers, respectively. E. coli harboring appY plasmids produced 2.8 mg lycopene/g dry cell weight (DCW), the same amount obtained with dxs despite the fact that appY is not directly involved in the lycopene synthesis pathway. The co-expression of appY, crl, and rpoS with dxs synergistically enhanced lycopene production. The co-expression of appY with dxs produced eight times the amount of lycopene (4.7 mg/g DCW) that was produced without expression of both genes (0.6 mg/g DCW). © 2005 Wiley Periodicals, Inc.</P>

Comparative Analysis of Lycopene Content in Tomato using a Microplate Reader and High-Performance Liquid Chromatography

( Shiva Ram Bhandari ),( Jun Gu Lee ) 전북대학교 농업과학기술연구소 2023 농업생명과학연구 Vol.54 No.2

Lycopene, a major carotenoid in tomato (Solanum lycopersicum L.), is a potent natural antioxidant and the main target in tomato breeding programs. A rapid and accurate method of lycopene quantification is required for the crop improvement program. Here, we aimed to determine the lycopene content in tomato fruits at different developmental stages using a micro-plate reader that reduces the time and cost from that needed for high-performance liquid chromatography (HPLC). For this, chloroform/methanol: 1/1 (v/v), n-hexane/ethanol: 1/1 (v/v), and n-hexane/acetone/ethanol: 2/1/1 (v/v/v) solvents were used separately for the extraction of lycopene. It was then centrifuged and syringe filtered, and the absorbance of aliquot was measured using HPLC. Lycopene content measured via HPLC and the micro-plate reader method at 470 and 503 nm was compared. The results showed a higher extraction efficiency for chloroform/methanol solvents (741-942 mg/kg) than for the different solvent systems (535-818 mg/kg). Both the 470 and 503 nm wavelengths showed similar lycopene content using HPLC. However, the 470 nm wavelength was more suitable than the 503 nm, as lycopene showed a higher response at 470 than at 503 nm. Similarly, lycopene measurement at 470 and 503 nm using 96-well micro-plate showed good linearity (y = 0.9959x) and a significant positive correlation (r = 0.9962), but the values at the early stages (green, breaker and pink) were overestimated at 470 nm. Comparative analysis of lycopene using HPLC at 470 nm and the 96-well micro-plate at 503 nm in tomatoes regardless of maturity stages showed good linearity, with a positive correlation coefficient (r = 0.9968). This method for the measurement of lycopene shows promise for widespread screening of the tomato germplasm in breeding programs for the selection of functional genotypes.

토마토 품종 및 부위별과 토마토 가공제품의 ascorbic acid, lycopene, ${\beta}$-carotene과 ${\alpha}$-carotene 함량 비교

최석현,김동호,김동석,Choi, Suk-Hyun,Kim, Dong-Ho,Kim, Dong-Seok 한국조리학회 2011 한국조리학회지 Vol.17 No.4

우수한 영양성분과 생리활성 물질을 함유하고 있는 토마토에 대해서 품종별 완숙 토마토의 부위 와 토마토 가공제품에 대하여 ascorbic acid, lycopene, ${\beta}$-carotene과 ${\alpha}$-carotene의 함량 차이 를 조사한 결과 토마토의 품종에 따른 ascorbic acid 함량 수준의 차이가 존재하며, 부위별에서는 pulp보다 jelly가 월등히 높은 함량 수준을 나타내었다. 토마토 가공제품의 ascorbic acid의 함량은 토마토 주스 제품들이 타 제품들보다는 ascorbic acid 함량 수준이 높았지만, 가공 시 토마토 이외에 첨가되는 다양한 첨가제의 영향에 따른 것으로 ascorbic acid 함량 수준에 따라 제품을 평가하기에는 다소 무리가 있었다. 품종별 lycopene의 함량은 품종별 pulp와 jelly 부위 간의 lycopene의 함량 수준은 일정한 차이가 존재하지 않음을 알 수 있었다. 대부분의 품종의 부위별 ${\beta}$-carotene의 함량 수준의 큰 차이를 나타내지 않았지만, 여용 품종은 pulp 부위 보다 jelly 부위가 2.7배 높은 함량 수준을 나타내었다. 품종별 ${\alpha}$-carotene의 함량은 pulp와 jelly 부위 모두 가장 높은 함량 수준을 나타내었으며, yellow carol 품종의 경 우 lycopene, ${\beta}$-carotene 함량의 경우 부위별과 상관없이 가장 낮은 함량 수준을 나타내었으며, ${\alpha}$-carotene의 경우는 pulp 및 jelly 부위 모두 검출되지 않았다. 토마토 가공제품별 lycopene, ${\beta}$-carotene, ${\alpha}$-carotene의 함량 수준은 전제적으로 유의성 있는 차이를 보였는데 이는 토마토 가공제품은 토마토의 가공 형태 및 첨가제로서의 역할에 따른 lycopene, ${\beta}$-carotene, ${\alpha}$-carotene의 함량 수준의 차이가 존재함을 알 수 있었다. 본 연구를 통하여 우수한 특성을 가진 토마토의 다양한 형태로의 섭취, 즉 국내에서 생육형태로만 섭취되어지고 있는 토마토에 대하여 다양한 토마토 가공제품과 함께 사용하여 영양적으로 우수한 다양한 조리법을 개발하는데 도움이 되고자 한다. For tomatoes containing valuable nutrients and biological active substances, this study examined differences in the ascorbic acid, lycopene, ${\beta}$-carotene, and ${\alpha}$-carotene contents in processed tomato products according to tomato cultivar and the part of fully ripened tomatoes. According to the results, the ascorbic acid content was different among tomato cultivars, and it was far higher in jelly than in pulp among the parts of tomatoes. The ascorbic acid content in processed tomato products was higher in tomato juice than in other types of tomato products, but the difference was mainly from various additives used in addition to tomatoes; therefore, it was somewhat unreasonable to compare the ascorbic acid content among the products. It was found that the lycopene content was not significantly different between pulp and jelly in each cultivar. In most of the cultivars, the ${\beta}$-carotene content was not significantly different according to the parts, but in cultivar yeoyong, the content was 2.7 times higher in jelly than in pulp. The ${\alpha}$-carotene content was highest in both pulp and jelly for all the cultivar, and the lycopene and ${\beta}$-carotene contents were lowest regardless of parts for cultivar Yellow Carol. ${\alpha}$ carotene was not detected in either pulp or jelly. The lycopene, ${\beta}$-carotene, and ${\alpha}$-carotene contents showed significant difference among processed tomato products, and the difference came mainly from the type of processing and additives. Tomatoes have superior characteristics, but they are usually consumed uncooked in Korea. Thus, this study aimed to contribute to the various forms of consumption of tomatoes, that is, the development of various nutritionally excellent cooking methods using processed tomato products.

RAW 264.7 세포에서 Lycopene의 MAPK/Nrf2/HO-1 신호 전달 체계를 통한 항산화 효과

박충무,안현,윤현서 대한통합의학회 2024 대한통합의학회지 Vol.12 No.1

Purpose: Lycopene is abundantly contained in Tomatoes and is known for diverse biological activities such as antioxidant, anti-inflammatory, and anticancer effects. In this study, the antioxidative potential of lycopene was investigated through the induction of hemeoxygenase (HO)-1 by nuclear factor-erythroid 2 p45-related factor2 (Nrf2) and upstream signaling molecules, mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)/Aktin RAW 264.7 cells. Methods: The antioxidative potential of lycopene against oxidative stress and its molecular mechanisms were determined by the cell viability assay, intracellular reactive oxygen species (ROS) formation assay, and Western blot analysis in RAW 264.7 cells. Results: Lycopene treatment significantly attenuated tert-butyl hydroperoxide (t-BHP) induced intracellular ROS formation in a dose-dependent manner without any cytotoxicity. In addition, 50 μM of lycopene for 6 h treatment induced potent HO-1 expression and its transcription factor, Nrf2. MAPK and PI3K/Aktwere also analyzed due to their critical roles in the regulation of cellular redox homeostasis against oxidative damage. As a result, phosphorylation of extracellular regulated kinase (ERK) was significantly induced by lycopene treatment while the activated status of c-Jun NH2-terminal kinase (JNK), p38, and Akt, were not given any effect. To confirm the antioxidative mechanism of HO-1 mediated by ERK activation, each selective inhibitor was employed in a protection assay, in which oxidative damage occurred by t-BHP. Lycopene, SnPP, and CoPP treatments reflected accelerated HO-1 expression could be a protective role against oxidative damage-initiated cell death. A selective inhibitor for ERK significantly inhibited the lycopene-induced cytoprotective effect but selective inhibitors for other signaling molecules did not attenuate the rate of t-BHP-induced cell death. Conclusion: In conclusion, lycopene potently scavenged intracellular ROS formation and enhanced the HO-1 mediated antioxidative potential through the modulation of Nrf2, MAPK signaling pathway in RAW 264.7 cells.

Effects of Dietary Lycopene Supplementation on Plasma Lipid Profile, Lipid Peroxidation and Antioxidant Defense System in Feedlot Bamei Lamb

Hongqin Jiang,Zhenzhen Wang,Yong Ma,Yanghua Qu,Xiao Nan Lu,Hailing Luo 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.7

Lycopene, a red non-provitamin A carotenoid, mainly presenting in tomato and tomato byproducts, has the highest antioxidant activity among carotenoids because of its high number of conjugated double bonds. The objective of this study was to investigate the effect of lycopene supplementation in the diet on plasma lipid profile, lipid peroxidation and antioxidant defense system in feedlot lamb. Twenty-eight Bamei male lambs (90 days old) were divided into four groups and fed a basal diet (LP0, 40:60 roughage: concentrate) or the basal diet supplemented with 50, 100, and 200 mg/kg lycopene. After 120 days of feeding, all lambs were slaughtered and sampled. Dietary lycopene supplementation significantly reduced the levels of plasma total cholesterol (p<0.05, linearly), total triglycerides (TG, p<0.05) and low-density lipoprotein cholesterol (LDL-C, p<0.05), as well as atherogenic index (p<0.001), whereas no change was observed in high-density lipoprotein cholesterol (p>0.05). The levels of TG (p<0.001) and LDL-C (p<0.001) were decreased with the feeding time extension, and both showed a linear trend (p<0.01). Malondialdehyde level in plasma and liver decreased linearly with the increase of lycopene inclusion levels (p<0.01). Dietary lycopene intake linearly increased the plasma antioxidant vitamin E level (p<0.001), total antioxidant capacity (T-AOC, p<0.05), and activities of catalase (CAT, p<0.01), glutathione peroxidase (GSH-Px, p<0.05) and superoxide dismutase (SOD, p<0.05). The plasma T-AOC and activities of GSH-Px and SOD decreased with the extension of the feeding time. In liver, dietary lycopene inclusion showed similar antioxidant effects with respect to activities of CAT (p<0.05, linearly) and SOD (p<0.001, linearly). Therefore, it was concluded that lycopene supplementation improved the antioxidant status of the lamb and optimized the plasma lipid profile, the dosage of 200 mg lycopene/kg feed might be desirable for growing lambs to prevent environment stress and maintain normal physiological metabolism.