황련해독탕이 수종의 인간 암세포 증식에 미치는 영향

성현경,민상연,김장현,Sung, Hyun Kyung,Min, Sang Yeon,Kim, Jang Hyun 대한한방소아과학회 2013 대한한방소아과학회지 Vol.27 No.1

Objectives The aim of this study is to investigate whether hwang-ryun-haedok-tang (HDT) affect proliferations of androgen-dependent LNCaP prostate cancer cells, androgen-independent PC-3, DU-145 prostate cancer cells, MCF-7 human breast cancer cells, A549, NCI-H292 human pulmonary cancer cells and K-562 human chronic myelogenous leukemia cells. Materials and Methods Effects of HDT on proliferations of each cancer cell line were investigated. 20,000 cells/well were plated in each well of 96-well culture plate. After 24 hrs, 0.01-10% of HDT in culture medium was added to cancer cells. The number of cells was counted by using SRB assay or direct cell counting method after 72 hours from drug treatment. Effect of baicalein or berebrine on proliferation was assessed according to the same method. Results (1) HDT inhibited proliferations of LNCaP, PC-3 and DU-145 prostate cancer cells. (2) HDT inhibited proliferation of MCF-7 breast cancer cells. (3) HDT also inhibited proliferations of A549, NCI-H292 pulmonary cancer cells and K-562 chronic myelogenous leukemia cells. (4) Baicalein and berberine also showed inhibitory effects on proliferations of prostate and breast cancer cells. Conclusion : HDT inhibited proliferations of human prostate, breast, pulmonary and blood cancer cells. These results suggest us the potential use of HDT as a chemopreventive or chemotherapeutic agent. Effect of HDT on human cancer should be further investigated using in vivo experimental models that can reflect pathophysiology of human cancer through another studies.

Inhibitory Effects of Kimchi Extracts on the Growth and DNA Synthesis of Human Cancer Cells

Hur, young-Mi,Kim, So-Hee,Park, Kun-Young The Korean Society of Food Science and Nutrition 1999 Preventive Nutrition and Food Science Vol.4 No.2

Effect of solvent extracts and juice supernatants from kimchis on the growth of various human cancer cells was studied, comparing with the actions on the normal cells. Inhibitory effect of kimchi extracts on[3H] thymidine incorporation n cancer cells was also investigated. The methanol extract, hexane extract and methanol soluble fraction (MSF) of 3-week fermented kimchi did not have growth inhibitory effect on Ac2F rat normal liver cells at the concentrations of 0.5~2%. However, marked decrease in the growth of AGS human gastric cancer cells was shown by the treatment of those extacts. The juice from the kimchi samples also suppressed the growth of K-562 human leukemia cells and MG-63 human osteosarcoma cells. Especially, the juice of 3-week fermented kimchi exhibited the strong growth inhibitory effect in MG-63 human osteosarcoma cells. At the photomicrographs, growth inhibition and morphological change of the cells treated with kimchi juice were observed. And the solvent extracts of 3-week fermented kimchi suppressed the growth of cancer morethan the extracts or juices from fresh and 6-week fermented kimchi. When AGS human gastric cancer cels were treated with the extracts of 3-week fermented kimchi, [3H] thymidine incorporation in the cells also decreased. These results showed that kimchi extracts and juices had growth inhibitory effects on human osteosarcoma, leukemia and gastric cancer cells, but had no toxicity to the normal cells. We suggest that kimchi might have anticancer effect in part due to inhibition of the growth and DNA synthesis of cancer cells.

백화사설초(白花蛇舌草), 산자고(山慈姑), 절패모(浙貝母)에 의한 MDA-MB-231 인체 유방암 세포에서의 항암 효과

진명호,박선영,강유경,심원석,허희수,홍상훈,박철,최영현,박상은,Jin, Myung-Ho,Park, Sun-Young,Kang, You-Gyung,Shim, Won-Suk,Hur, Hee-Soo,Hong, Sang-Hoon,Park, Cheol,Choi, Yung-Hyun,Park, Sang-Eun 대한한방내과학회 2014 大韓韓方內科學會誌 Vol.35 No.2

O. diffusa, C. appendiculata and F. thunbergii are reported to possess many pharmacological activities including anti-oxidant, anti-inflammatory, anti-hypertension, anti-diabetic and anti-cancer effects. However, their anti-cancer activities in human breast cancer have not been clearly elucidated yet. Objectives: In the present study, we compared the in vitro cytotoxic effects of single and complex treatment of O. diffusa, C. appendiculata and F. thunbergii in human breast cancer MDA-MB-231 cells. Methods: After we treated human breast cancer MDA-MB-231 cells with O. diffusa, C. appendiculata and F. thunbergii. we evaluated viability, growth inhibition, morphological changes, apoptotic body formation, measurement of the cell cycle and formation of DNA fragmentation of these cells. Results: We found that single treatment of O. diffusa and F. thunbergii could inhibit cell proliferation in human breast cancer MDA-MB-231 cells. However, complex treatment of O. diffusa, C. appendiculata and F. thunbergii had weak or no effect on the cell proliferation of MDA-MB-231 cells. The first, anti-proliferative effects of O. diffusa in MDA-MB-231 cells was associated with G2/M arrest of cell cycle and apoptotic cell death. The second, anti-proliferative effect of F. thunbergii in MDA-MB-231 cells was associated with apoptotic cell death. Conclusions: Taken together, these findings suggest that O. diffusa and F. thunbergii may be a potential chemotherapeutic agent for the control of human breast cancer cells, further studies will be needed to identify the molecular mechanisms.

The effects of human milk proteins on the proliferation of normal, cancer and cancer stem like cells

Kang, Nam Mi,Cho, Ssang-Goo,Dayem, Ahmed Abdal,Lee, Joohyun,Bae, Seong Phil,Hahn, Won-Ho,Lee, Jeong-Sang The Korean Society of Analytical Sciences 2018 분석과학 Vol.31 No.6

Human breast milk (HBM) provides neonates with indispensable nutrition. The present study evaluated the anti-cancer activity of diluted and pasteurized early HBM (< 6 weeks' lactation) on human breast cancer cell lines. The cell lines MCF7 and MDA-MB231 were exposed to 1 % HBM from the 1st, 3rd, and 6th weeks of lactation and exhibited reduced proliferation rates. As controls, breast cell lines (293T and MCF-10A), breast cancer cell lines (MCF-7 and MDA-MB-231), and $CD133^{hi}CXCR4^{hi}ALDH1^{hi}$ patient-derived human cancer stem-like cells (KU-CSLCs) were treated with prominent milk proteins ${\beta}$-casein, ${\kappa}$-casein, and lactoferrin at varying doses (10, 50, and $100{\mu}g$) for 24 or 48 hrs. The impact of these proteins on cell proliferation was investigated. Breast cancer cell lines treated with ${\kappa}$-casein and lactoferrin exhibited significantly reduced viability, in both a dose- and time-dependent manner. Interestingly, ${\kappa}$-casein selectively impacted only cancer (but not normal breast) cell lines, particularly the more malignant cell line. However, ${\beta}$-casein-exposed human breast cancer cell lines exhibited a significantly higher proliferation rate. Thus, ${\kappa}$-casein and lactoferrin appear to exert selective anti-cancer activities. Further studies are warranted to determine the mechanisms underlying ${\kappa}$-casein- and lactoferrin-mediated cancer cell-selective cytotoxic effects.

Arrest of Cell Growth by Inhibition of Endogenous Reverse Transcription Activity in Cancer and Somatic Cell Lines

김미정,이성호,박종근,전병균 한국생명과학회 2024 생명과학회지 Vol.34 No.6

The present study assessed the cytotoxic effects on cell growth and senescence in human cancer (A-549, AGS, HCT-116, MDA-MB-231, and U 87-MG) and normal (MRC-5 and mesenchymal stem cells) cell lines treated with efavirenz (EFA), an inhibitor of non-nucleoside reverse transcriptase (RTase). Following EFA treatment, the half-maximal inhibitory concentration (IC50) values were approximately 15 μM, and the IC50 value was significantly (p<0.05) lower in the cancer cell lines, compared to normal cell lines. After determining the IC50 values against EFA, each cell line was treated with 15 μM EFA for up to one week. Significant (p<0.05) decreases in endogenous RTase and telomerase activity were observed in the cancer cell lines. RTase and telomerase activity were absent or detected at very low levels in both EFA-untreated and treated MRC-5 and MSC normal cells. The cell doubling time (CDT) was also significantly (p<0.05) prolonged by the decreased cell growth rate in the EFA-treated cancer cell lines compared to the untreated cell lines. Furthermore, EFA-treated cancer cells displayed a high number of cells with a high intensity of senescence-associated ß-galactosidase activity (SA-ß-gal activity), compared to the untreated cells. The present study showed that inhibition of RTase activity induces cellular senescence and arrests cell growth in human cancer cell lines; however, normal cell lines showed greater tolerance against EFA. RTase treatment could offer optional chemotherapy for cancer treatment in human cancer cell lines with high RTase activity.

The effects of human milk proteins on the proliferation of normal, cancer and cancer stem like cells

강남미,조쌍구,아브달아메드,이주현,배성필,한원호,이정상 한국분석과학회 2018 분석과학 Vol.31 No.6

Human breast milk (HBM) provides neonates with indispensable nutrition. The present study evaluated the anti-cancer activity of diluted and pasteurized early HBM (< 6 weeks’ lactation) on human breast cancer cell lines. The cell lines MCF7 and MDA-MB231 were exposed to 1% HBM from the 1st, 3rd, and 6th weeks of lactation and exhibited reduced proliferation rates. As controls, breast cell lines (293T and MCF-10A), breast cancer cell lines (MCF-7 and MDA-MB-231), and CD133hiCXCR4hiALDH1hi patient-derived human cancer stem-like cells (KUCSLCs) were treated with prominent milk proteins β-casein, κ-casein, and lactoferrin at varying doses (10, 50, and 100 μg) for 24 or 48 hrs. The impact of these proteins on cell proliferation was investigated. Breast cancer cell lines treated with κ-casein and lactoferrin exhibited significantly reduced viability, in both a dose- and time-dependent manner. Interestingly, κ-casein selectively impacted only cancer (but not normal breast) cell lines, particularly the more malignant cell line. However, β-casein-exposed human breast cancer cell lines exhibited a significantly higher proliferation rate. Thus, κ-casein and lactoferrin appear to exert selective anti-cancer activities. Further studies are warranted to determine the mechanisms underlying κ-casein- and lactoferrin-mediated cancer cell-selective cytotoxic effects.

사람 구강암세포 KB에서 microRNA 발현 분석

모임염 ( Shin Yeob Mo ),김정선 ( Jeong Sun Kim ),조선호 ( Seon Ho Cho ),박종태 ( Jong Tae Park ),유선경 ( Sun Kyung Yu ),김도경 ( Do Kyung Kim ) 조선대학교 치의학연구원 2014 Oral Biology Research (Oral Biol Res) Vol.38 No.2

Purpose: The main aim of this study was to compare and analyze expression profiles of miRNAs to establish miRNA-related cancer cell growth inhibition in normal human oral keratinocytes (NHOK) and KB human oral cancer cells. Materials and Methods: Expression profiles of miRNAs in NHOK and KB cells were examined by miRNA microarray analysis, quantitative real-time polymerase chain reaction analysis (qRT-PCR), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and gene array analysis. Results: In the miRNA microarray analysis, 164 and 149 miRNAs were up- and down-regulated, respectively, in KB cells compared to NHOK among 1,769 miRNAs examined. miR-30a, miR-99a, and miR-155 were up-regulated more than 10-fold in KB cells compared to NHOK, whereas miR-205, miR-203, and miR-200c were down-regulated more than 10-fold. In qRT-PCR analysis, expression levels of miR-30a, miR-99a, and miR-155 increased in KB cells compared to NHOK more than 15-fold, whereas miR-205, miR-203, and miR-200c were down-regulated more than 10-fold. Importantly, overexpression of miR-205 and miR-203 significantly inhibited growth of KB cells. In gene array analysis, 3,154 and 2,709 genes were up- and down-regulated more than 2-fold, respectively, in miR-205-overexpressing KB cells compared to control KB cells. Overexpressed miR-203 in KB cells induced expression of 2,707 genes and decreased that of 2,352 genes. Conclusion: These results show that miR-205 and miR-203 expression was reduced in KB cells compared to NHOK, and proliferation of KB human oral cancer cells was inhibited. Moreover, these in vitro results indicate that miR-205 and miR-203 have significant therapeutic potential as molecular medicine for treatment of oral cancer by turning on silenced tumor suppressor genes through miRNA targeting.

Molecular Phylogenetic Analysis of the Human Endogenous Retrovirus E (HERV-E) Family in Human Tissues and Human Cancers

Yi, Joo-Mi,Kim, Heui-Soo The Genetics Society of Japan 2007 Genes & genetic systems Vol.82 No.1

<P>The human genome is estimated to contain up to 50 copies of full-length and truncated members of HERV-E family. They are thought to be involved in human gene transcription. Here we examine the expression pattern and phylogenetic relationships of the HERV-E in diverse human tissues and cancer cells using RT-PCR amplification and bioinformatic tools. The <I>env</I> gene was expressed in many human tissues (brain, prostate, testis, kidney, placenta, spleen, thymus and uterus) but not in heart, liver, lung and skeletal muscle, importantly, HERV-E expression was detected in all cancer cell lines examined (RT4, PFSK-1, BT-474, HCT-116, TE-1, UO-31, Jurkat, HepG2, A549, MCF7, OVCAR-3, MIA-PaCa-2, PC3, LOX-IMVI, AZ521, 2F7, U-937 and C-33A), suggesting that HERV-E family are expressed corresponding to the transcriptional program of human tissues and human cancer cells. Phylogenetic analysis of HERV-E <I>env</I> family from human tissues, cancer cells and our previous data identify two groups (I and II) through evolutionary divergence. Taken together, HERV-E family expression in human tissues and human cancer cells exhibited close relationships of the <I>env</I> gene sequences across human chromosomes. These active HERV-E elements deserve further investigation as potential pathogenic factors in human diseases such as cancers.</P>

Inhibition of Cell Growth by Anoikis in Various Human Cancer Cell Lines Treated with an Extract of Smilax china L.

Min-Jae Kim(김민재),Hyeon-Ji Kim(김현지),Moo-Gyeong Kim(김무경),Sung-Ho Lee(이성호),Byeong-Gyun Jeon(전병균) 한국생명과학회 2021 생명과학회지 Vol.31 No.3

본 연구에서는 다양한 사람의 암세포주(A-549, MCF-7, MDA-MB-231, U87-MG, AGS, MKN-74 및 SNU-601 세포)와 정상세포주(MRC-5 섬유아세포 및 사랑니 유래 중간엽성 줄기세포에 토복령 추출물(Smilax china L. extract, SCLE)을 처리하여 세포 사멸 효과를 조사하였다. SCLE 처리 후, MTT 분석에서 여러 암세포주는 정상세포주보다 유의적으로 휠씬 낮은 반억제농도값을 나타내었고, 세포는 세포부착력의 소실로 인한 세포사멸(anoikis)이 관찰되었다. 또한, SCLE를 처리한 A-549, AGS 및 MCF-7 암세포주에서 세포의 생존성과 말단소립 복원효소의 활성도를 조사하였을 때, SCLE 처리 후 4일째에 세포의 생존성과 말단소립 복원효소의 활성도가 현저히 줄어드는 것을 관찰하였다. 또한, SCLE를 처리한 A-549, AGS 및 MCF-7 암세포주에서 세포 주기의 G1기에서 세포 성장이 정지되었고,세포 사멸이 유의적으로 증가하는 것을 알 수 있었다. 그러나, SCLE 처리는 rho 단백질의 활성과 관련 없는 세포부착력의 소실과 세포 사멸이 유도되는 것을 관찰하였다. 이 연구의 결과를 바탕으로 토복령 추출물은 정상 세포보다는 암세포에 특이적으로 세포부착력의 소실과 세포 사멸을 유도하여, 이 추출물에 포함된 물질을 이용한 항암 연구에 응용될 수 있을 것으로 판단된다. The present study examined the cytotoxic effects of a Smilax china L. extract (SCLE) in human cancer (A-549, MCF-7, MDA-MB-231, U87-MG, AGS, MKN-74, and SNU-601) and normal MRC-5 fibroblasts, as well as in mesenchymal stem cells derived from dental tissue (DSC). The 50% inhibitory concentration (IC50) values for SCLE were significantly (p<0.05) lower in the cancer cell lines (A-549, MCF-7, MDA-MB-231, U87-MG, AGS, MKN-74 and SNU-601) than in the MRC-5 and DSC cells. Cell growth was significantly (p<0.05) more inhibited in the cancer cell lines treated with 200 μg/ml SCLE than in the normal MRC-5 and DSC, and anoikis-like floating cell morphology was observed in the SCLE-treated cancer cells. The cells detached by SCLE treatment were retrieved daily and assayed for viability and telomerase activity. Cells retrieved at 4 days showed significantly decreased viability and telomerase activity (p<0.05), as well as apoptosis-like abnormal morphology, when compared to cells retrieved in the previous 3 days. The ratio of apoptosis and cells in the G1 phase was significantly (p<0.05) increased in the A-549, AGS, and MCF-7 cancer cells treated with SCLE for 4 days compared to untreated controls. However, after SCLE treatment, cell adhesion was not increased by application of an inhibitor of the associated protein kinase (ROCK) that mainly contributes to the increase in cell attachment. This suggests that the cellular detachment by SCLE is probably controlled by a Rho-independent mechanism(s). These observations indicate that SCLE readily induces anoikis in cancer cells and could serve as a potent agent for cancer chemotherapy.

Inhibitory Effect of Kale Juice on the Growth and DNA Incorporation of Human Cancer Cells

Lee, Seon-Mi,Park, Kun-Young The Korean Society of Food Science and Nutrition 1997 Preventive Nutrition and Food Science Vol.2 No.2

The inhibitory effects of kale juice on the growh and DNA incorporation of human cancer cells, using HT-29 colon cancer cells, MG-63 osteosarcoma cells, AGS gastric adenocarcinoma cells and K-562 leukemia cells, were studied. The growth of human cancer cells were inhibited in the presence of kale juice (10, 20 nd 40$\mu$l/ml) and the effects were the juice concentration- and incubation time-dependent up to 6 days. When 20$\mu$l/ml of kale juice was added to the media of HT-29, MG-63, AGS and K-562 cancer cells, the cell growth after 6 or 4 days of incubation was retarded by 83~95% of control group. Morphological changes of HT-29 colon cancer cells wre studied under inverted microscope. As the concentration of kale juice increased up to 20$\mu$l/ml, degree of cell aggregation was decreased. Moreover, the DNA incorporation o AGS gastric adenocarcinoma cells and MG-63 osteosarcoma cells which were labeled with [$^3$H] thymidine was significantly reduced after 2 days of incubation at 37$^{\circ}C$ with kale juice. Therefore, we concluded that kale juice strongly decreased the growth of various human cancer cells.