골담초근의 생리활성 -고지질, 고혈당 및 간손상에 미치는 영향-

김학선,김일혁,Kim, Hak-Sun,Kim, Il-Hyuk 한국생약학회 1992 생약학회지 Vol.23 No.2

The studies were attempted to evaluated the therapeutic effects of various fractions(ether, methanol, butanol) of Caragana chamlagu roots on the hyperlipemia induced by feeding the diet containing 1%, cholesterol and 0.5%, cholic acid in rats, and on the hyperglycemia induced by streptozotocin in rats. Also the preventive effects of these fractions were studies on the liver damage in $CCl_4-intoxicated$ rats. The followings were obtained as the results: 1.The butanol fraction was significantly shown to down the serum lipid level in 1%, cholesterol and 0.5%, cholic acid diet-feeding rats and streptozotocin-induced hyperglycemic rats. Cholesterol level in $CCl_4-intoxicated$ rats was reduced in the case of all pre-treated groups. 2.The serum glucose level of streptozotocin-induced hyperglycemic rats was significantly decreased by the administration of various fractions of C. chamlagu roots, and the lipid-peroxidation of pancreas was significantly decreased in the case of administration of these fractions. 3.The activates of s-GOT and s-GPT were decreased by the administration of various fractions, especially in butanol fraction, of C. chamlagu roots in the $CCl_4-intoxicated$ rats. The liver lipid-peroxidation was decreased by administration of 200mg/kg of these fractions in the $CCl_4-intoxicated$ rats. In histological observation, hepatic cellular necrosis and fatty acid deposit were increased remarkably by $CCl_4-intoxication$, but the pretreatment of various fractions of C. chamlagu roots improved the pathological change of parenchymatous cell necrosis and fatty change around centrilobular area of the control.

地骨皮 EA分劃의 CCl₄損傷肝에 대한 保護作用

曺永周,金聖勳 대한동의병리학회 1997 동의생리병리학회지 Vol.11 No.2

肝에 對한 東醫學的 實驗 硏究는 肝이 代謝物質에 의한 中毒에 敏感하게 反應하여 肝細胞의 變性, 壞死, 脂肪蓄積, 肝酵素의 漏出 등의 肝障碍가 나타나는 점에 着眼하여 肝毒性 誘發 物質인 CCl₄등의 肝損傷 病態 모델을 이용한 損傷肝에 대한 保護作用 實驗이 主를 이루고 있고, 最近에는 韓藥 혹은 韓方處方의 抽出物에서 補肝作用을 나타내는 主要 物質이 多糖類임이 實驗的으로 밝혀져 이에 對한 硏究도 활발히 進行되고 있다. 이에 본 연구는 대표적인 補肝藥物이라고 할 수 있는 拘杞子의 根皮인 地骨皮가 肝·肺·賢經에 入하고 淸熱凉血 作用이 있음을 勘案하여, in vitro에서 肝保護作用을 screening 하였던 바, 地骨皮 ethyl acetate(EA)層이 가장 有效하여, 本 實驗에서는 in vivo에서 四鹽化炭素(CCl₄)로 肝損傷을 誘發시킨 後 地骨皮 ethyl acetate層과 地骨皮 ethyl acetate層을 다시 SiO₂chromatograph 方法을 利用하여 分離한 細分劃(F-1, F-2, F-3, F-4)을 試科로 使用, 經口投與 後 肝機能과 相關된 各種 血淸分析 및 肝組機檢査 등을 實施하였다. CCl₄中毒 肝損傷에서 albumin과 cholesterol値는 對照群에 비하여 地骨皮 ethyl acetate層 投與群과 DDB 投與群에서 有意性있게 增加되었으며, alanine aminotransferase와 aspartate aminotransferase値는 對照群에 비하여 地骨皮 ethyl acetate層 投與群과 DDB 投與群에서 有意性있게 減少되었다. lactate dehydrogenase値는 對照群에 비하여 DDB 投與군에서 有意性있게 減少되었고, 地骨皮 ethyl acetate層 投與群에서 對照群에 비하여 減少되었으나, 有意性은 나타나지 않았다. 中毒에 의한 肝組織 變化에서는 正常群에 비하여 對照群에서 中心靜脈과 門脈周圍에서 공포성 변성이 나타나고, 細胞內의 核이 濃縮되며, 中性球의 浸潤이 뚜렷하게 나타났으며, 地骨皮 ethyl acetate層에서 投與群은 DDB 投與群 정도로 나타나 肝細胞의 損傷을 抑制하였다. Ethyl acetate層에서 특히 F-3와 F-4 분획 投與群이 對照群에 比하여 ALT, AST에서 모두 有意性있게 減少하였으며, 특히 F-3 投與群이 더욱 有效하였다. In order to evaluate the protective effect of ethyl acetate layer and its subfractions (F-1, F-2, F-3, F-4) of Lycii Cortex Radix (LCREA) on hepatic damage induced by CCl₄, the study was done. The blood chemistry and histological study were done following oral administration with materials. The results were obtained as follows: 1. In the hepatocytotoxicity with CCl₄, serum albumin and cholesterol were significantly increased in LCREA and DDB-treated groups as compared with the data of control. 2. In the hepatocytotoxicity with CCl₄, serum alanine aminotransferase (ALT) and aspartate aminotransferase(AST) were significantly decreased in LCREA and DDB-treated groups as compared with the data of control. 3. In the hepatocytotoxicity with CCl₄, serum lactate dehydrogenase (LDH) tended to decrease in LCREA-treated group with no significance while with significance in DDB-treated group as compared with control. 4. In histological changes of murine liver treated by CCl₄, LCREA inhibited the hepatic damage by CCl₄such as vacuolar degeration, lipid infiltration and pyknosis up to the efficacy by DDB as compared with control. 5. In the hepatocytotoxicity with CCl₄, serum alanine aminotransferase (ALT) and aspartate aminotransferase(AST) were significantly decreased in F-3 and F-4 treated groups as compared with the data of control and also F-3 subfraction was more effective. It was concluded that LCREA had protective effects on the hepatic damage, and especially F-3 isolated from LCREA was more effective, which suggest it is necessary to study more with F3 of Lycii Cortex Radix.

수도권 주변 소나무의 조직피해와 생장억제

이창석,길지현,유영한 한국생태학회 1998 Journal of Ecology and Environment Vol.21 No.2

Histological damage and growth inhibition of Pinus densiflora were analysed in different areas around the Metropolitan area of Seoul urban (heavily polluted), suburban (lightly polluted), and rural(unpolluted) areas. Soil properties of each area were also investigated. Contact angles of water droplet on needle leaves growing in polluted areas were lower than that in unpolluted area. Transpiration rates of needle leaves growing in polluted areas were more rapid than that in unpolluted area. These results represented that needle leaves growing in polluted areas were more susceptible to water deficit than that growing in unpolluted area was. Growths of annual ring of Pinus densiflora growing in polluted areas were lower than that in unpolluted area. On the other hand, soil pH in polluted areas was lower than that in unpolluted area. That is, the former was more acidified than that the latter was. Ca and Mg contents in polluted areas were lower than that in unpolluted area, while Al contents in polluted areas were higher than that in unpolluted area. These soil properties revealed that the effects of acid precipitates in urban and suburban areas were severer than that in rural area.

Ulmus macrocarpa Hance Reduces Cyclophosphamide-induced Toxicity in Mouse Liver

Deok Won Kim(김덕원),Kyung Tae Chung(정경태) 한국생명과학회 2021 생명과학회지 Vol.31 No.2

Cyclophosphamide (CP)는 면역 억제제 뿐만 아니라 암 및 림프종 등의 치료에 널리 사용된다. CP는 DNA 알킬화제로서, 간세포에서 대사되어 4-hydrocyclophosphamide (4H-CYP)와 aldophosphamide로 분리된다. Ulmus macrocarpa Hance는 부종, 유방염, 종양 및 기타 염증성 질환에 사용되어 왔다. 이 연구의 목적은 CP의 부작용에 대하여 U. macrocarpa Hance 열수 추출물이 조직학적 수준에서 CP의 부작용에 대한 간과 신장의 보호 기능과 U. macrocarpa Hance 자체의 잠재적 독성 영향을 조사하고자 하였다. 마우스 모델을 사용하여 헤마톡실린 및 에오신(H&E) 염색과 DAPI 염색으로 간과 신장을 조직학적으로 분석하였다. CP 처리한 마우스에서 간세포의 형태는 글리코겐 축적을 나타내지 않았고, 세포 밀도도 감소하였다. 그러나 UMWE+CP 처리군에서는 간세포의 형태와 세포 밀도는 정상 간세포 패턴과 유사하였다. 또한, UMWE으로만 처리한 마우스에서도 간세포의 형태와 세포밀도는 정상 간세포와 유사했다. 신장의 경우에는 정상 마우스와 비교했을 때 H&E 염색으로는 CP 또는 UMWE 처리된 마우스의 신장에서 명백한 차이를 나타내지 않았다. 즉, U. macrocarpa Hance의 열수추출물은 간과 신장에 아무런 영향을 유발하지 않으면서 CP가 유발한 독성을 감소시키는것으로 요약된다. 따라서 U. macrocarpa Hance는 제약 산업에 사용될 수 있는 가능성을 나타내었다. Cyclophosphamide (CP) is widely used in cancer and lymphoma treatments and as an immunosup-pressant drug. CP is a DNA alkylating agent that metabolizes into 4-hydrocyclophosphamide (4H-CYP) and aldophosphamide in hepatocytes. However, its metabolites cause DNA synthesis disorder, leading to apoptosis and toxic side effects. The development of technology to minimize this side effect is essential to improve CP’s clinical application. Various bioactive compounds have been reported to have anti-cancer and antioxidant functions and preventive or therapeutic roles in metabolic diseases. Many researchers have attempted to minimize the side effects and improve the efficacy of these drugs together with the use of bioactive compounds. Ulmus macrocarpa Hance has been used for the treatment of edema, mastitis, stomach pain, tumors, cystitis, and other inflammatory diseases. The aim of this study was to investigate at the histological level the protective function of U. macrocarpa Hance against CP’s side effects and any potential toxic effect of U. macrocarpa Hance in the liver and kidney. Water extracts of U. macrocarpa Hance reduced CP-induced toxicity and did not induce any histological damage in the liver and kidney. Therefore, U. macrocarpa Hance would be applicable in the pharmaceutical industry.

인공산성빗물이 소나무의 조직, 수분수지 및 생장에 미치는 영향

이창석,길지현,유영한 한국생태학회 1998 Journal of Ecology and Environment Vol.21 No.2

To clarify the effects of acid precipitates on histological damage, water status, and growth of Pinus densiflora green house experiment applyin simulated acid rain was carried out. Contact angle of water droplet on needles of P. densiflora seedlings treated with simulated acid rain of different pHs simulated acid rain was, the more rapid transpiration was. Leaf water potential after water withdrawal was also reduced rapidly in proportion to acidity of simulated acid rain. Height growth of P. densiflora seedlings treated with simulated acid rain of pH 2 decreased, while growth of seedlings treated with that of pH 3 and 4 increased comparing with that treated with normal rain of pH 5.6. pH of cultivated soil in pH 2 plot was acidified with the amount of simulated acid rain applied but that in pH 3 and 4 plots did not show any directional change. From those results, it could be interpreted that decrease of height growth in pH 2 plot was originated from multiple effects of water deficit from rapid transpiration and soil acidification. On the other hand, increased of height growth in pH 3 and 4 plots would be originated from the supply of N and S included in simulated acid rain.

Gastroprotective Effect and Antioxidant Properties of Different Laurus nobilis L. Leaf Extracts

Ester Speroni,Rinaldo Cervellati,Stefano Dall’Acqua,Maria Clelia Guerra,Emanuela Greco,Paolo Govoni,Gabbriella Innocenti 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.5

Laurus nobilis L. (Family Lauraceae) is an evergreen tree widely distributed in the Mediterranean area and Europe. It is used in folk medicine of different countries as a stomachic and carminative as well as in treatment of gastric diseases. Extracts obtained with different methods (methanol and chloroform) from laurel leaves were evaluated for their gastroprotective activities in the rat. The antioxidant capacity of the different extracts has been also measured in vitro. In order to confirm the activities investigated, histological observations were performed. The gastric damage was significantly reduced by all extracts administered. The more effective protection was produced by chloroformic and methanolic crude extracts. The results obtained after oral administration of L. nobilis leaf extracts are in good agreement with their antioxidant capacity, confirming the relationship between pharmacological efficacy and antiradical activity. Histological evidences confirm the results evaluated with the animal procedures.

질식성 심정지 모텔에서 N6-L-phenylisopropyl Adenosine의 영향

박제영,이미진,오동렬,이원재,김세경 대한응급의학회 2002 大韓應急醫學會誌 Vol.13 No.2

질식성 심정지 모델에서 N^6-L-phenylisopropyl Adenosine의 영향

박제영,이미진,오동렬,이원재,김세경 대한응급의학회 2002 대한응급의학회지 Vol.13 No.2

Purpose: Cardiac arrest and resuscitation produce global cerebral ischemia and reperfusion injury to the brain, which lead to high mortality and delayed neuronal death. Adenosine has been suggested as an endogenous neuroprotective molecule, acting through multiple potential mechanisms. We investigated the possible neuroprotective effects of adenosine on cerebral recovery following global ischemia induced by asphyxial cardiac arrest. Methods: Twenty-four rats were randomized into three groups. Group Ⅰ,Ⅱ and Ⅲ had anesthesia, procedures, and asphyxia for 7 minutes and then survived to 72 hours. Group Ⅰ(n=8) was not administered N^6-L-phenylisopropyl adenosine(L-PIA). Group Ⅱ(n=8) was administered L-PIA(0.8 mg/kg), and group Ⅲ(n=8) was administered L-PIA (1.5 mg/kg), after spontaneous circulation. The dose-dependent neuroprotective effects of L-PIA were compared to the control by using a histopathological method. Results: Histological observations of CA1 showed a more significant reduction of neuronal cell loss in groups Ⅱ and Ⅲ than in group I(p<0.05). Histological observations of CA2 and CA3 didn't show a significant reduction of neuronal cell loss in groups Ⅱ and Ⅲ compared to group Ⅰ. Conclusion: These results show that post-ischemic administration of adenosine protected against delayed neuronal damage in the hippocampal CA1 area following a 7-min asphyxial cardiac arrest in rats.

Investigation of a Photothrombosis Inducing System for an Observation of Transient Variations in an in vivo Rat Brain

Oh, Sung Suk,Park, Hye Jin,Min, Han Sol,Kim, Sang Dong,Bae, Seung Kuk,Kim, Jun Sik,Ryu, Rae-Hyung,Kim, Jong Chul,Kim, Sang Hyun,Lee, Seong-jun,Kang, Bong Keun,Choi, Jong-ryul,Sohn, Jeong-woo Optical Society of Korea 2018 Current Optics and Photonics Vol.2 No.6

For the spatiotemporally aligned observation of photothrombosis induction and transient variations of in vivo brain stroke, we developed a novel photothrombosis inducing system compatible to a magnetic resonance imaging (MRI) system using nonmagnetic stereotaxic equipment. From the spatial point of view, the system provides a more reliable level of reproducibility of the photothrombosis in each brain. From the temporal point of view, from T1- and T2-weighted in vivo MR (magnetic resonance) images, the transient variations such as incidence, location, and size of the thrombosis are measured quantitatively. In addition, the final variation is observed in the ex vivo brain by TTC (Triphenyltetrazolium chloride) staining based on histological assay and utilized for the verification of the MR images. From the experimental result of the rat brain, the proposed system shows more reliable characteristics for transient variations of brain strokes.