류마티스 질환에서 혈청 Soluble Fas Ligand (sFasL), FasL-Fas 복합체, FasL-IgG 복합체 측정

민준기 ( Jun Ki Min ),민소연 ( So Youn Min ),조미라 ( Mi Ra Cho ),정재연 ( Jae Yeon Jeong ),주대명 ( Dae Myung Jue ),민도준 ( Do June Min ),조철수 ( Chul Soo Cho ),김호연 ( Ho Youn Kim ) 대한류마티스학회 2000 대한류마티스학회지 Vol.7 No.4

Objective: To quantify the soluble Fas ligand (sFasL) and to measure FasL-Fas complex and FasL-IgG complex in the sera of patients with various rheumatic diseases: systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), systemic sclerosis (SSc), and adult onset Still`s disease (AOSD). Methods: Serum samples were obtained from 37 patients with SLE, 40 with RA, 30 with SSc, 20 with AOSD, and 40 healthy controls. The serum sFasL, FasL-Fas complex, and FasL-IgG complex were measured using a sandwich enzyme-linked immunoabsorbent assay. Hospital medical records were retrospectively reviewed for clinical and laboratory characteristics in patients with SLE. Disease activity in SLE patients was assessed by the SLE Disease Activity Index (SLEDAI) score. Results: In patients with SLE, serum sFasL levels (383.1±208.9pg/ml) were significantly higher (p<0.001) than those of healthy controls (192.0±84.7pg/ml). sFasL levels in patients with RA (150.8±30.7pg/ml, p=0.014), SSc (115.4±13.5pg/ml, p<0.001), and AOSD (137.5±12.9pg/ml, p=0.001) were significantly lower compared with healthy controls. The frequencies of positive FasL-Fas complex and FasL-IgG complex were higher in patients with SLE (56.8%, 56.8% respectively) than in healthy controls (2.5%, 0% respectively) (p<0.001). All patients with RA or AOSD were negative for FasL-Fas complex and FasL-IgG complex. No patients with SSc were positive for FasL-Fas complex. On the other hand, the positive frequency of FasL-IgG complex was greater in patients with SSc (16.7%) than in healthy controls (0%) (p=0.012). Serum levels of FasL-IgG complexes in active SLE patients (OD 0.467±0.050) were tended to be lower than those in inactive SLE patients (OD 0.509±0.055) (p=0.060). SLEDAI score was tended to be negatively correlated with the serum levels of FasL-IgG complex in patients with SLE (r=-0.308, p=0.068). Conclusion: These results suggest that FasL may possibly play a role in the pathogenesis of SLE.

Effectiveness of Carnitine-Orotate Complex on Aminotransferase Activity in Patients with Various Metabolic Diseases: A Retrospective Nationwide Real Life Cohort Study

( Dae Won Jun ),( Jun Young Song ),( Woo Seog Kim ),( Sung Gye Lee ),( Jong Sin Lee ),( Eun Kyung Baek ),( Ki San Joo ),( Myung Sook Yoon ),( Young Lim ),( Yook Kim ),( Il Kwon Park ),( Young Cheol Ju 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Previous reports demonstrated that carnitine restore mitochondrial and liver function in various liver diseases. We aimed to assess various metabolic profiles after 3 months carnitine-orotate complex (Godex^Ⓡ) treatment in patients with metabolic disease. Methods: We reviewed the records of 544 outpatients (type 2 diabetes, dyslipidemia, and hypertension) from 31 primary or secondary care clinics from January 2015 to May 2016 in Korea. The key inclusion criteria were such as following; i) participants who were prescribed antidiabetic, antihypertension, or lipid-lowering drugs, ii) elevated alanine aminotransferase activity more than 40 IU/L, iii) who were newly prescribed carnitine-orotate complex. Biochemical parameters (liver enzyme, glycated hemoglobin, lipid profile) as well as anthropometric markers observed for 3 months. Results: There was no significant weight change between before and after treatment with carnitine-orotate complex (71.55±12.19 kg vs. 71.1±11.8 kg, P=0.5367). After 3 months of combined treatment with carnitine-orotate complex, the rates of normalization of serum ALT and AST levels were 68.2 % and 63.2%, respectively. Mean change in serum ALT levels from before at 3 months was -44.07±36.15 IU/L (P<0.0001) while it was -33.28±39.46 IU/L in serum AST levels (P<0.0001). Carnitine-orotate complex showed improvement in HbA1c level with 0.74 % decrease (P<0.0001) in diabetes. Serum triglyceride level significantly decreased 54.22 mg/dl compare to baseline in dyslipidemic subjects (P<0.0001). Systolic and diastolic blood pressure also significantly decrease in hypertensive subjects (-6.84 mmHg vs. -3.86 mmHg, respectively, P<0.0001). Conclusions: Three months of treatment with carnitine-orotate complex effectively decreased liver enzyme in patients with various metabolic diseases.

Incidence and Intensity of Root Disease Complex due to Nematode and Soilborne Fungal Pathogens in Mulberry (Morus alba L.)

Naik, Vorkady Nishitha,Sharma, Dinesh Dutta,Govindaiah, Govindaiah Korean Society of Sericultural Science 2008 International Journal of Industrial Entomology Vol.16 No.2

A preliminary survey on the incidence and intensity of root disease complex (association of Meloidogyne incognita and root rot pathogens) was carried out in the sericultural areas of Karnataka. A total of 280 mulberry gardens were surveyed in 14 districts of Karnataka belonging to different types of soil (red sandy, red loamy and black cotton), farming systems (irrigated and rainfed), varieties (V-1, K-2, Local and S-13) and age of the plants (0-5, 5-10 and 10-15 years). It was observed that the association of M. incognita with Botryodiplodia theobromae and Fusarium solani causes the root disease complex in mulberry. Of the 280 gardens visited, 94 were infested with the disease complex and incidence was recorded as 33.6%. The higher intensity of root disease complex was observed when the root system had more than 100 galls/plant with infection of mixed population of B. theobromae and F. solani in sandy soil under irrigated farming. The 5-10 years old mulberry plantation with V-I variety was found to be most susceptible to root disease complex. Districts like Mysore, Kolar, Mandya, Tumkur, Chitradurga and Bangalore were observed as sensitive areas. Further, the wounds caused by M. incognita in mulberry roots favour the easy entry of root rot pathogens, which increased the severity of the disease very fast.

Biological control of the complex disease caused by the root-knot nematode and the fungal pathogen

Young Ho Kim,Seon-Hye Son 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10

It is well known that the root-knot nematodes, Meloidogyne spp., incite and aggravate the diseases caused by fungal and bacterial pathogens. The synergistic effects of the inoculation of Meloidogyne incognita combined with Fusarium oxysporum f. sp. lycopersici showed the greatly increased wilt symptoms developed on tomato plants compared to the inoculation of either of the two pests alone. For the biological control of the complex disease, a variety of bacterial isolates were tested for antagonistic effects to select ones that had both nematicidal and antifungal activities. Among forty plant growth-promoting rhizobacteria (PGPR) tested, Paenibacillus polymyxa G508 and G462 and P. lentimorbus G158 showed strong antifungal and nematicidal activities against F. oxysporum f. sp. lycopersici and second-stage juveniles (J2) of M. incognita, respectively, and also inhibited egg hatch of the nematode. The addition of Paenibacillus strains into potted soil suppressed the Fusarium-wilt severity and root galling on tomato and increased plant growths. P. lentimorbus G158 were abundantly proliferated on tomato seeds and hypocotyls more than P. polymyxa G 462 and had no phytotoxic effect on tomato plant. Under the greenhouse conditions, seed treatment of P. lentimorbus G158 reduced wilt severity caused by Fusarium wilt-root knot disease complex and root gall formation and increased tomato growth compared to the untreated control. Root-galls caused by both pathogens treated with bacterial culture had fewer and smaller giant-cells than untreated control, and scanning electron microscopy revealed alteration and distortion of hyphal cell wall of F. oxysporum and lysis of M. incognita egg shell by the bacterial treatment. All of these results suggest the Paenibacillus strains, especially G158 may have a high potential developed as biological control agents for the complex disease.

심혈관계 질환 보유 고령 여성들의 복합 트레이닝 프로그램이 심혈관계 질환 위험 요소 및 변화율에 미치는 영향

곽이섭(Yi Sub Kwak),김덕중(Duk-Jung Kim) 한국생명과학회 2008 생명과학회지 Vol.18 No.5

본 연구는 CVD를 보유한 고령 여성들의 12주간 규칙적인 저항성 운동을 병행한 유산소 트레이닝 프로그램 참여가 안정시와 운동중 CVD 위험 요소에 미치는 효과와 이에 따른 위험 요소들의 변화율에는 어떠한 양상을 보이는지를 규명하고자 하였다. 측정 항목은 12주간 트레이닝 프로그램 참여 전ㆍ후 최대하 운동부하 검사 시 CVD 위험 요소와 트레이닝 전후 위험 요소들의 변화율을 측정하였다. 연구 결과, 안정 시 CVD 위험 요소에 있어서 생활습관은 가족력의 빈도가 높았고, 체지방, 혈청지질, CRP는 통제 집단이 증가한 반면, 운동 집단은 12주간 트레이닝 프로그램 참여 후에 감소하였다. 변화율에 있어서, 통제 집단은 변화율이 증가한 반면, 운동 집단은 신체조성, 혈청 지질, 혈당 및 CRP가 감소하였다. 운동 시 CVD 위험 요소에 있어서도 운동 집단은 12주간 프로그램 참여 후에 유의한 향상 효과를 나타냈다. 변화율에 있어서, 운동 집단은 SBP, MVO₂, ST slope은 감소하였고, VO₂, 운동 시간은 증가하였다. 결론적으로, CVD 보유 고령자들의 체계적인 신체활동의 참여는 CVD 위험 요소의 유지보다는 향상의 결과를 가져올 수 있으며, CVD 위험 요소의 변화율에 있어서도 통제 집단에 비해 운동 집단이 효과적으로 잘 관리되므로 고령 여성들의 복합 트레이닝은 CVD 합병증을 예방하고 보다 긍정적인 향상의 효과를 기대할 수 있다. The purpose of this study was to investigate the effects of the regular resistance exercise with aerobic exercise training program participation on cardiovascular disease risk factors and rate of changes in the elderly women with cardiovascular disease. Subjects were 40 elderly women with cardiovascular disease who were 65~75 years old. We enrolled 20 exercise group into the participated 12 week complex exercise program. In measurement index, rest, exercise cardiovascular disease risk factor and rate of changes were measured by the submaximal exercise stress test. Results of this study were as follows; in the rest cardiovascular disease risk factor, % body fat, serum lipids and C-reactive protein (CRP) were increased in control group, but exercise group were decreased. In the exercise cardiovascular disease risk factor, control group were decreased, but exercise group were increased by the 12 week training program. In conclusion, we could know that not only the regular training might increase or change almost the cardiovascular function variables for serum lipid, body composition, exercise capacity between before and after complex training. Therefore, we might tell that complex training program could provide an excellent parameters for enforcing or keeping the cardiovascular function of elderly women with cardiovascular disease.

집합 결합과 신경망을 이용한 복합질환의 예측

최현주,김승현,위규범 한국정보처리학회 2008 정보처리학회논문지. 소프트웨어 및 데이터 공학 Vol.15 No.4

Since complex diseases are caused by interactions of multiple genes, traditional statistical methods are limited in its power to predict the onset of a complex disease. Recently new approaches using machine learning techniques are introduced. Neural nets are a suitable model to find patterns in complex data. When large amount of data are fed into a neural net, however, it takes a long time for learning and finding patterns. In this study we suggest a new model that combines the set association, which is a statistical technique to find important SNPs associated with complex diseases, and neural network. We experiment with SNP data related to asthma to test the effectiveness of our model. Our model shows higher prediction accuracy and shorter execution time than neural net only. We expect our model can be used effectively to predict the onset of other complex diseases. 복합질환은 다수의 유전자들이 상호작용하여 유발되는 질병으로서, 여러 유전자들이 관여한다는 복잡성 때문에 전통적인 분석 방법을 적용하는데 한계가 있다. 최근에는 기계학습 기법을 이용한 새로운 분석 방법들이 제안되고 있다. 신경망은 이처럼 복잡한 데이터에서 일정한 패턴을 찾아 이를 분류하는데 적합한 모델이다. 그러나 다량의 데이터가 입력으로 들어오는 경우에 학습에 오랜 시간이 걸리고 패턴을 찾기가 어려워지는 단점이 있다. 본 연구에서는 다량의 SNP 데이터로부터 질병에 연관된 소수의 중요 SNP을 찾기 위한 통계학적인 방법인 집합결합(set association)과 신경망을 결합한 모델을 제시한다. 이 모델을 천식 관련 SNP 데이터에 적용하여 천식 발병 여부를 예측한 결과, 신경망만 사용했을 때보다 실행 시간도 빠르고 예측 정확도도 높았다. 이 모델은 다른 복합질환의 예측에도 효과적으로 사용할 수 있을 것으로 기대한다.

e-헬스 리터러시 및 인포그래픽 유형에 대한 선호도 연구 - 20대 남녀를 중심으로 -

강로빈 사단법인 한국브랜드디자인학회 2023 브랜드디자인학연구 Vol.21 No.2

Crohn's disease, which shows the highest prevalence in both men and women in their 20s, disease prevention and early detection are necessary. e-health literacy was found to affect the knowledge level of disease and the ability to understand health information online. In this study, preference for Crohn's disease infographic types were investigated, and the difference in preference according to e-health literacy was analyzed. For the research method, 8 types of infographics were produced according to the degree of complexity based on Feather Complexity and Design Complexity, and e-Health literacy and infographic preference were investigated in June 2022 for 134 people in their 20s. As a result of the study, the average score of e-health literacy was 3.50 (±0.54) out of 5. The preference for Crohn's disease infographic was the highest in type 7 with high feature complexity and design complexity. As a result of t-testing to analyze the difference in e-health literacy according to preference, there was no statistically significant difference in e-health literacy according to infographic type preference. However, as a result of cross-analysis to find out the correlation between gender and infographic type preference, males appeared to be most preferred type 3 and 4, which are high FC among low DC types, females appeared to be most preferred type 7, which is high DC and high FC types. 남녀 모두 20대에서 가장 높은 유병률을 나타내고 있는 크론병은 질병 예방 및 조기 발견을 위해 건강교육이 필요하다. e-헬스 리터러시는 질병에 대한 지식수준과 인터넷 건강정보 이해 능력에 영향을 미치는 것으로 나타났다. 본 연구에서는 크론병 인포그래픽 제작을 위한 예비 조사로서 크론병 인포그래픽 유형에 대한 선호도를 알아보고 e-헬스 리터러시에 따른 차이를 분석하였다. 연구 방법은 Feature Complexity와 Design Complexity를 바탕으로 복잡성 정도에 따라 총 8개의 유형의 인포그래픽을 제작하였으며, 20대 134명을 대상으로 2022년 6월 한 달간 e-헬스 리터러시와 인포그래픽 선호도를 조사하였다. 연구 결과, e-헬스 리터러시는 5점 만점에 평균 3.50(±0.54)점으로 나타났다. 크론병 인포그래픽의 선호도는 Feature Complexity와 Design Complexity가 높은 7번 유형이 가장 높았으며, 선호도에 따른 e-헬스리터러시의 차이를 분석하고자 t-검증을 시행한 결과, 선호도에 따른 e-헬스 리터러시는 통계적으로 유의한 차이가 나타나지 않았으나 성별과 인포그래픽 유형 선호도 간의 연관성을 알아보기 위해 교차분석을 실시한 결과 남자는 낮은 DC 유형 중 높은 FC인 3, 4유형을 가장 많이 선호했고, 여자는 높은 DC 유형에서 높은 FC유형인 7번 유형을 가장 많이 선호하는 것으로 나타났다.

대구 안심연료단지 환경오염물질 노출 평가(3) -원소 탄소, 결정형 실리카 및 안정동위원소비를 이용한 오염원 기여율 및 분포특성-

정종현 ( Jong Hyeon Jung ),피영규 ( Young Gyu Phee ),손병현 ( Byung Hyun Shon ),배혜정 ( Hye Jeong Bae ),양원호 ( Won Ho Yang ),김지영 ( Ji Young Kim ),김근배 ( Geun Bae Kim ),최종우 ( Jong Woo Choi ),박성준 ( Sung Jun Park ),이 한국산업보건학회 2015 한국산업보건학회지 Vol.25 No.3

Objectives: This study measured and analyzed the concentrations of crystalline silica, elemental carbon and the contribution ratio of pollutants which influence environmental and respiratory disease around the Ansim Briquette Fuel Complex in Daegu, Korea. Methods: We analyzed the crystalline silica and elemental carbon in the air according to FTIR(Fourier Transform Infrared Spectroscopy) and NIOSH(National Institute of Occupation Safety and Health) method 5040, respectively. In addition, lead stable isotopes, and carbon and nitrogen stable isotopes were analyzed using MC-ICP/MS(Multi Collector-Inductively Coupled Plasma/Mass Spectrometer), and IRMS(Isotope Ratio Mass Spectrometer), respectively. Results: The concentration of crystalline silica in the direct exposure area around the Ansim Briquette Fuel Complex was found to be 0.0014±0.0005 ㎎/S㎥, but not to exceed the exposure standards of the ACGIH(American Conference of Governmental Industrial Hygienists). In the case of the autumn, the direct exposure area was found to show a level 2.5 times higher than the reference area, and on the whole, the direct exposure area was found to have a level 1.4 times higher than the reference area. The concentration of elemental carbon in the direct exposure area and in the reference area were found to be 0.0014±0.0006 ㎎/S㎥, and 0.0006±0.0003 ㎎/S㎥, respectively. This study confirmed the contribution ratio of coal raw materials to residentially deposited dusts in the area within 500 meters from the Ansim Briquette Fuel Complex and the surrounding area with a stable isotope ratio of 24.0%(0.7-62.7%) on average in the case of carbon and nitrogen, and 33.9%(26.6-54.1%) on average in the case of lead stable isotopes. Conclusions: This study was able to confirm correlations with coal raw materials used by the Ansim Briquette Fuel Complex and the surrounding area. The concentration of some pollutants, crystalline silica, and elemental carbon emitted to the direct-influence area around the Ansim Briquette Fuel Complex were relatively higher than in the reference area. Therefore, we need to impose continuous and substantive reduction countermeasures in the future to prevent particulate matter and coal raw materials in the study area. It is time for the local government and authorities to prepare active administrative methods such as the relocation of Ansim Briquette Fuel Complex.

연령대별 복합요인과 신체활동량의 변화가 만성질환의 수준과 의료비에 미치는 영향

지창진(Chang-Jin Ji) 대한고령친화산업학회 2023 대한고령친화산업학회지 Vol.15 No.2

연구목적 본 연구에서 4년 동안 연령대별 신체활동량 변화에 따른 의료비용 개연성을 살펴보고 해당 연구결과를 토대로 만성질환 패턴을 분석하고자 한다. 특정 연령대의 질환 발생을 예방하기 위한 정책 수립과 함께 만성질환 개선 사례와 생활습관의 관련성을 고찰하여 의료 정책 개발에 적용될 수 있는 대안을 제시하고자 한다. 연구방법 한국의료 패널데이터 ver. 1.71 를 활용하여 각각의 연령대별로 만성질환 위험도 및 의료비지출을 확인하기 위해 4년동안 설문지에 참여한 인원(7,184명)을 데이터 추출 후 종단 연구 분석을 실시하였다. 연령대별 신체활동량과 좌업 시간의 개별요인에 대해 기술 통계를 t-test 와 one-way ANOVA를 실시하였으며, 만성질환별 위험도를 살펴보기 위해 복합 요인들은 1분위(“High PA, Low Sit”,기준변수)와 비교하여, 2분위(High PA, High Sit), 3분위(Low PA, Low Sit), 4분위(Low Sit, High PA)에 따른 로지스틱회귀분석을 실시하였다. 연구결과 첫째, 장년층과 노년층 신체활동량은 정상그룹에 비해 단순만성질환에서 복합만성질환으로 갈수록 감소하였으며, 노년층은 의료비지출 평균이상 그룹에서 신체활동량 감소가 나타났다. 둘째, 노년층 좌업 시간은 정상그룹에 비해 단순만성질환에서 복합만성질환으로 갈수록 증가하였다. 셋째, 복합요인(신체활동과 좌업 시간)에서 고혈압과 당뇨병 위험도는 저위험군(1분위)에 비해 고위험군(4분위)에서 상승하였으며, 뇌혈관 질환 위험도는 저위험군(1분위)에 비해 좌업 시간이 높은 그룹(2분위,4분위)에서 상승하는 것이 나타났다. 심장질환 위험도는 저위험군에 비해 모든 그룹(2분위,3분위,4분위)에서 위험도가 상승이 나타났다. 결론 데이터 기반한 치료의학과 함께 예방의학 차원의 접근(운동처방 및 관리를 위한 지역별 전문기관 선정을 통한 네트워크 신체활동 프로그램)이 필요하다. 장년층 세대부터는 의료비 감소를 위해 선제적 만성질환 추적 플랫폼 시스템 개발 및 구축을 고민해야 할 시점이라 생각된다. Objective : The purpose of this study was to confirm the relationship between the disease degree and the lifestyle in the cases. Methods : Using Korean Health Panel Survey Data ver. 1.71 to determine the risk of chronic disease by age group, the complex factors, longitudinal study analysis was conducted for the data was extracted from the participants who participated in the 4 years questionnaire(n=7,184). Results : First, Age(50-64) and Age(over 65) group PA decreased from simple chronic disease to complex chronic disease compared to the normal group. Age(over 65) showed PA decrease in the group above the average medical expenditure. Second, compared to the normal group, the Sit time of the elderly increased from simple chronic disease to complex chronic disease. Third, in complex factor the risk increased of Hypertension and Diabetes odds in the high-risk group compared to the low-risk group, and the risk of Cerebrovascular Disease odds in the high sit grop compared to the low-risk group. Cardiovascular risk increased in all groups compared to low-risk group. Conclusion : Along with data-based therapeutic medicine, a preventive medicine-level approach is needed.

Research Articles : Incidence and Intensity of Root Disease Complex due to Nematode and Soilborne Fungal Pathogens in Mulberry (Morus alba L.)

( Vorkady Nishitha Naik ),( Dinesh Dutta Sharma ),( Govindaiah ) 한국잠사학회 2008 International Journal of Industrial Entomology Vol.16 No.2

A preliminary survey on the incidence and intensity of root disease complex (association of Meloidogyne incognita and root rot pathogens) was carried out in the sericultural areas of Karnataka. A total of 280 mulberry gardens were surveyed in 14 districts of Karnataka belonging to different types of soil (red sandy, red loamy and black cotton), farming systems (irrigated and rainfed), varieties (V-1, K-2, Local and S-13) and age of the plants (0-5, 5-10 and 10-15 years). It was observed that the association of M. incognita with Botryodiplodia theobromae and Fusarium solani causes the root disease complex in mulberry. Of the 280 gardens visited, 94 were infested with the disease complex and incidence was recorded as 33.6%. The higher intensity of root disease complex was observed when the root system had more than 100 galls/plant with infection of mixed population of B. theobromae and F. solani in sandy soil under irrigated farming. The 5-10 years old mulberry plantation with V-1 variety was found to be most susceptible to root disease complex. Districts like Mysore, Kolar, Mandya, Tumkur, Chitradurga and Bangalore were observed as sensitive areas. Further, the wounds caused by M. incognita in mulberry roots favour the easy entry of root rot pathogens, which increased the severity of the disease very fast.