성견 치주인대세포의 줄기세포 특성 연구

김경화,김수환,설양조,이용무,Kim, Kyoung-Hwa,Kim, Su-Hwan,Seol, Yang-Jo,Lee, Yong-Moo 대한치주과학회 2007 Journal of Periodontal & Implant Science Vol.37 No.3

In spite of the attention given to the study of mesenchymal stem cells derived periodontal ligament (PDL), there is a lack of information about canine PDL cells. In this study, we characterized canine PDL cells to clarify their stem cell properties, including self renewal, proliferate rate, stem cell markers and multipotency. PDL cells were obtained from extracted premolars of canines, following a colony forming assay and proliferation rate of sub-confluent cultures of cells for self-renewal, immunostaining for STRO-1 and CD146/MUC18 and a differentiation assay for multipotency. Canine PDL cells formed single-cells colonies and 25% of the PDL cells displayed positive staining for BrdU. The cells expressed the mesenchymal stem-cell markers, STRO-1 and CD146/MUC18. Under defined culture conditions, the cells differentiated into osteoblasts and adipocytes, but the cells didn't differentiated into chondrocytes. The findings of this study indicated that the canine PDL cells possess crucial stem cells properties, such as self-renewal and multipotency, and express the mesenchymal stem cell markers on their surface. The isolation and characterization of canine PDL cells makes it feasible to pursue preclinical models of periodontal regeneration in canine.

곤충사료 급여에 따른 반려견들의 기호성 평가(1)

최인학,정연우,박관호,정태호 한국환경과학회 2023 한국환경과학회지 Vol.32 No.11

This study assesses the palatability of regular canine diets and seven types of black soldier fly-based canine diets when fed to dogs. Sixteen dogs of two types were included in this study: 8 poodles (average weight 2.7 kg ± 0.5) and 8 bichons frises(average weight 2.0 kg ± 0.5). For intake and first choice, two-bowl tests, adhering to standards of canine palatability, were conducted every two days for a total of 14 days by comparing between the control and each treatment. Data, including total intake and total first choice were collected and accumulated for a total of 58 days. This encompassed 14 days of data on comparison between control and treatments, and 44 days of data on comparison among treatment groups (e.g., T1 vs T2) of black soldier fly-based canine diets. Significance differences in canine palatability was observed in treatments (p<0.05), except for the control and T2 results. Among the two-bowl tests, T1 and T2 exhibited the lowest intake and first choices. In particular, the palatability of canine diets ranked in the order T6 > T3 > T7 > T4 > T5 compared to each control. The total intake demonstrated in the following ranking: T6 > T3 > T7 > T5 > Control > T4 > T2 > T1. The total first choice was highest for T6, followed by T3, T7, T5, T4, Control, T2, and T1. In conclusion, insect diets with higher protein content such as T6, T3, and T7 representing as black soldier fly-based canine diets exhibit higher intake and first choice preferences in canines.

미맹출 견치의 특발성 근심 이동

최아미,송제선,이제호,최형준,최병재,김성오 大韓小兒齒科學會 2013 大韓小兒齒科學會誌 Vol.40 No.1

상악 견치는 이소 맹출이나 매복이 빈번히 관찰되는 치아로, 그 가능성을 조기에 발견했을 때에, 자연 개선 가능성을 고려 한 합리적이고 체계적인 대처가 가능해진다. 체계적인 대처라 함은, 일단 주기적으로 관찰 하다가 개선되지 않을 경우 견치의 정상적인 맹출 시기에 인접하여 유견치를 발치해서 다시 한번 자연 개선을 기다려보는 것인데, 이 때 추가적으로 공간 유지 장치나 부차적인 개창술을 고려해 볼 수도 있을 것이다. 단, 너무 어린 나이에 유견치를 발치하면, 발치와가 단단한 경조직으로 채워지며 오히려 맹출 경로가 막힐 수 있 으므로, 정상 맹출 시기의 약 6개월 전 정도에 발치하는 것이 바람직 하다. 이렇게 해도 자연 개선 되지 않는다면, 가급적 견 치의 치근이 완성되기 전에, 외과적으로 버튼을 접착하고 올바른 위치로 교정적 견인하는 방법을 고려해 볼 수 있다. 환자가 늦게 내원했거나, 적극적 치료가 늦어 이미 위치가 심하게 변위 됐거나, 인접한 절치의 치근 흡수가 진행된 경우, 외과적 발치 술 혹은 향후 보철적 치료가 추가될 것이다. 본 증례는 매복된 상악 견치의 특발성 악화 현상을 관찰하고 보고하는 바이다. Maxillary canines are the most commonly impacted or ectopically erupted teeth. If we find the abnormality of maxillary canines early, we can manage it reasonably and systematically. If we cannot see the spontaneous normalization at the periodic recall, primary canine extraction will be the next treatment choice. However, if the primary canine is extracted too early, the extraction socket will be filled with hard bone and then the eruption pathway can be locked. So it is more beneficial to extract the primary canine at the period about 6 months before the normal canine eruption time. The next treatment plan can be surgical and orthodontic approaches before the root apical closure of the impacted canine. Sometimes, surgical extraction and further prosthetic procedure can be needed for a severely malposed impacted canine or badly resorpted incisor. This is the case of the idiopathic mesial movement of impacted maxillary canines.

Clinical predictors of potentially impacted canines in low-risk patients: A retrospective study in mixed dentition

Sergio Estelita Barros,Bianca Heck,Kelly Chiqueto,Eduardo Ferreira 대한치과교정학회 2023 대한치과교정학회지 Vol.53 No.2

Objective: To evaluate the null hypothesis that there is no difference in a set of clinical predictors of potentially impacted canines between low-risk patients with and without displaced canines. Methods: The normal canine position group consisted of 30 patients with 60 normally erupting canines ranked in sector I (age, 9.30 ± 0.94 years). The displaced canine group comprised 30 patients with 41 potentially impacted canines ranked in sectors II to IV (age, 9.46 ± 0.78 years). Maxillary lateral incisor crown angulation, inclination, rotation, width, height, and shape, as well as palatal depth, arch length, width, and perimeter composed a set of clinical predictors, which were evaluated on digital dental casts. Statistical analyses consisted of group comparisons and variable correlations (p < 0.05). Results: There was a significant association between sex and mesially displaced canines. Unilateral canine displacement was more prevalent than bilateral displacement. The crown of the maxillary lateral incisors was significantly angulated more mesially and rotated mesiolabially in low-risk patients with displaced canines, who also had a shallower palate and shorter anterior dental arch length. Lateral incisor crown angulation and rotation, as well as palatal depth and arch length, were significantly correlated with the canine displacement severity. Conclusions: The null hypothesis was rejected. Maxillary lateral incisor angulation inconsistent with the “ugly duckling” stage as well as a shallow palate and short arch length are clinical predictors that can significantly contribute to the early screening of ectopic canines in low-risk patients.

Cone-beam computed tomography analysis of the association between sella turcica bridging and palatally impacted canine

Soo-Min Gil,Jae-Sung Park,Sang-Cheol Kim 대한치과의사협회 2020 대한치과의사협회지 Vol.59 No.1

The aim of this study was to analyze the association between sella turcica bridging (STB) and palatally impacted canine and to investigate the correlation between the angular measurements of palatally impacted canine and STB using cone beam computed tomography (CBCT). The impaction group included 36 subjects (16.97 ± 6.00 years) who had at least one palatally impacted canine and the control group consisted of 36 subjects (16.89 ± 3.52 years) who had normally erupted canines without any dental anomalies. Sella turcica and dental measurements were obtained from reconstructed CBCT images using OnDemand3D 1.0software. Sella turcica dimensions were compared between two groups, and the STB ratio, the proportion of interclinoid distance to diameter of sella turcica, were compared between the impacted and non-impacted sides. Correlation coefficients were calculated between the angular measurements of palatally impacted canine and STB ratio. In the impaction group, interclinoid distance and STB ratio were significantly smaller than those of the control group (p < 0.001). The distribution of STB type II, partial calcification of intercliniod ligaments, was significantly higher in the impaction group than in the control group (p < 0.05). However, there was no significant difference of STB between the impacted and non-impacted sides (p > 0.05) in the unilateral impaction group. Further, STB ratio was negatively correlated with the mesiodistal angulation and the buccolingual inclination of the palatally impacted canine (p < 0.05). The STB was more frequently found in patients with palatally impacted canine, and the degree of STB was significantly associated with the angular measurements of impacted canine.

Gene expression analysis of 46 cancer associated genes by high throughput real-time qPCR in canine: Facilitate the biomarker identification

Sunirmal Sheet(Sunirmal Sheet ),Hana Kim(Hana Kim),Minjeong Ko(Minjeong Ko),Nayeon Kim(Nayeon Kim),서경원(Kyoung-Won Seo),Bong-Hwan Choi(Bong-Hwan Choi),이경태(Kyung-Tai Lee) 한국예방수의학회 2022 예방수의학회지 Vol.46 No.1

The prevalence of cancer in companion dogs is growing nowadays with the increasing worldwide population of domestic dogs. Since there is a less established standard of care in veterinary medicine, investigational treatments, such as the development of biomarkers can be considered as a therapeutic intervention for early diagnosis. Despite the enormous efforts that have been invested in the search of biomarkers, still, there is a need for easy detection of significant biological markers for predicting canine cancers at an early stage. In this study, we have analyzed the expression pattern of previously reported 46 canine cancer-associated candidate genes in blood specimens using real-time qPCR. We hypothesized that analysis of gene expression in blood would provide preliminary evidence of local or systemic immunogenic response which further contribute to the easy and early diagnosis of canine cancer from blood specimen as an analytical tool. The datasets included a total of 22 blood samples collected from different breeds of dogs diagnosed with cancer and five from healthy normal dogs. RT-qPCR analysis was performed by employing the SYBR Green PCR mix to assess the expression of these 46 genes in a total of 27 samples. From our result, a total of nine genes (ROS1, C1QA, CD48, IL1b, TLR2, IL2R, CHI3L1, CTSS, and TLR7) were found to be significantly up-regulated (p < 0.05 and p < 0.01) in the cancer samples compared to non-cancer samples. The relative expression level of ROS1, C1QA, CD48, IL1b, TLR2, IL2R, CHI3L1, CTSS, and TLR7 genes was 5.74, 4.78, 3.94, 2.94, 2.57, 2.53, 2.50, 2.04, and 2.57, respectively, in cancer samples compared to non-cancer samples. Thus, our results reveal several highly expressed cancer genes that can be therapeutic target genes for further testing in canine cancers.

매복견치의 맹출유도

이난영(Nan-Young Lee) 대한치과의사협회 2012 대한치과의사협회지 Vol.50 No.6

Permanent canine are very important teeth in point of function as well as esthetic. Therefore impaction of canines is a frequently encountered clinical problems. The incidence of impaction ranges between 1-3%. The cause of canine impaction can be result of ectopic position of tooth germ, defect of dental follicle, early loss or delayed exfoliation primary canine, space problem, supernumarary teeth, cyst, odontoma. Impaction frequently involves further complication such as root resorption of adjacent teeth, cyst formation and migration of the neighboring teeth. Various treatment modalities include extraction of primary canine, surgical opening and orthodontic traction, autotransplantation, surgical extraction of impacted canine.

수평 매복 상악 견치의 교정적 견인 치험례

박해지(Hai-Ji Park),박정진(Jung-Jin Park),강윤구(Yoon-Goo Kang) 대한치과교정학회 2022 대한치과교정학회 임상저널 Vol.12 No.1

Maxillary canine is one of the most frequently impacted teeth after third molar. This case report presents two patients with horizontal impacted maxillary canines treated by orthodontic traction. A 10-year old boy presented with a chief complaint of the impacted maxillary left canine and a 9-year old boy was referred with bilateral impacted maxillary canines. Impacted canines of both patients showed labially directed horizontal angulation. Impacted canines were treated and aligned by twice sequential surgical exposures. After treatment, both cases showed favorable aligning and root parallelism of the impacted canine.

매복된 상악 견치에 의한 전치의 치근 흡수에 대한 후향적 연구

정서현,송지수,신터전,현홍근,김영재,김정욱,이상훈,장기택,Jung, Seo-Hyun,Song, Ji-Soo,Shin, Teo Jeon,Hyun, Hong-Keun,Kim, Young-Jae,Kim, Jung-Wook,Lee, Sang-Hoon,Jang, Ki-Taeg 대한소아치과학회 2018 大韓小兒齒科學會誌 Vol.45 No.2

Root resorption of the permanent maxillary incisors can occur due to ectopic eruption of the permanent canines. Severe root resorption threatens the long-term survival of the affected incisors. The aim of the present study was to identify risk factors for root resorption of the maxillary incisors associated with impacted maxillary canines. In the present study, we retrospectively analyzed the Cone-Beam Computed Tomography (CBCT) scans of 65 children and adolescents with ectopically erupting maxillary canines (total of 88 impacted canines). Root resorption of central incisors was significantly associated with the mesiodistal position and root development of the adjacent canine. Root resorption of lateral incisors was significantly associated with sex, age, and the buccolingual and vertical position of the adjacent canine. However, enlargement of the dental follicle was not significantly associated with root resorption of adjacent incisors. Although incisor resorption is difficult to diagnose and predict, our findings suggest that changes in the dental follicles of the erupting maxillary canines do not cause resorption of the adjacent permanent incisors. CBCT should be utilized to ensure early diagnosis of impacted canines and precise evaluation of incisor root resorption. 상악 견치가 매복 시에 종종 인접 전치 치근의 치근흡수가 나타난다. 심각한 전치의 치근 흡수는 치아의 장기적인 예후를 불량하게 하기도 한다. 이 연구의 목적은 3차원 영상을 이용하여 상악 견치에 의한 전치의 치근 흡수를 관찰하고 관련된 요인을 분석하는 것이다. 연구 대상자는 상악 견치 매복을 가진 65명의 어린이로 총 88개의 치아에 대해 65개의 cone-beam computed tomography 영상을 분석하였다. 그 결과, 상악 견치의 치근 발달 정도와 치근단공의 폐쇄 및 치관의 근원심적인 위치가 중절치의 치근흡수와 유의한 관계를 나타내였다. 측절치의 치근 흡수 여부는 환아의 성별, 나이, 상악 견치의 협설 위치, 수직적 위치에 영향을 받았다. 상악 견치 치배의 확장은 중절치와 측절치의 치근 흡수 여부와 유의한 관계가 없어, 치배의 확장이 치근 흡수에 직접적인 역할을 하지 않는 것으로 나타났다. 상악 견치의 매복 시에 전치의 치근 흡수는 예측하기 어렵기 때문에, 3차원 영상을 이용한 조기 진단이 필요하다.

Rapid palatal expansion for the treatment of an ectopically erupting maxillary canine: case reports

장수영,김지연,박기태 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.4

Maxillary canine impaction is an anomaly often encountered in children. Although it has been reported that the incidence of palatally impacted canines is higher than that of labially impacted ones, it has been found that labial impaction of canines is more common than palatal impaction in Asian populations. In the cases presented here, maxillary canines were guided normally after rapid palatal expansion, followed by modification of root angulation of neighboring lateral incisors in 8-10-year-old children who had maxillary canines suspected of labial impaction. Consequently, the method of modifying the root angulation of the maxillary lateral incisor, combined with rapid palatal expansion, is effective in preventing impaction of an ectopically erupting maxillary canine without resorting to surgical methods. 상악 견치 매복은 소아 청소년기 환자들에게서 흔하게 발견되는 문제이다. 일반적으로 구개측 매복이 순측 매복 보다 더 흔한 것으로 알려져 있지만 서양에서는 오히려 순측 매복이 더 많은 것으로 보고되고 있다. 본 보고에서는 파노라마 방사선 사진상 상악 견치의 순측매복이 의심되는 8-10세의 혼합치열기 아동을 대상으로 급속 구개확장(rapid palatal expansion) 을 시행하였으며, 이를 통하여 상악 측절치의 근원심 치근각도를 조절하여 매복 가능성이 있는 견치의 정상맹출을 유도할 수 있었기에 보고하는 바이다.