Biodegradation of Aliphatic and Aromatic Hydrocarbons by Nocardia sp. H17-1

Baek, Kyung-Hwa,Yoon, Byung-Dae,Oh, Hee-Mock,Kim, Hee-Sik,Lee, In-Sook TAYLOR & FRANCIS LTD 2006 GEOMICROBIOLOGY JOURNAL Vol.23 No.5

<P> We investigated the biodegradation of hydrocarbon components by Nocardia sp. H17-1 and the catabolic genes involved in the degradation pathways of both aliphatic and aromatic hydrocarbons. After 6 days of incubation, the aliphatic and aromatic fractions separated from Arabian light oil were degraded 99.0 ± 0.1% and 23.8 ± 0.8%, respectively. Detection of the catabolic genes involved in the hydrocarbon degradation indicated that H17-1 possessed the alkB genes for n-alkane biodegradation and catA gene for catechol 1,2-dioxygenase. However, H17-1 had neither the C23O gene for the degradation of aromatic hydrocarbons nor the catechol 2,3-dioxygenase activity. The investigation of the genes involved in the biodegradation of hydrocarbons supported the low degradation activity of H17-1 on the aromatic fractions.</P>

Complete genome of Zhongshania aliphaticivorans SM-2^T, an aliphatic hydrocarbon-degrading bacterium isolated from tidal flat sediment

Jia, B.,Jeong, H.I.,Kim, K.H.,Jeon, C.O. Elsevier Science Publishers 2016 Journal of biotechnology Vol.226 No.-

<P>Zhongshania aliphaticivorans SM-2(T), a degrader of aliphatic hydrocarbons, is a Gram-negative, rod-shaped, flagellated, facultatively aerobic bacterium. Here, we report the genome sequence of strain SM-2(T), which has a size of 4,204,359 bp with 44 tRNAs, 9 rRNAs, and 3664 protein-coding genes. In addition, several genes encoding aliphatic hydrocarbon degraders (alkane 1-monooxygenase, haloalkane dehalogenase, and cytochrome P450) were detected in the genome shedding light on the function of pollutants degradation. (C) 2016 Elsevier B.V. All rights reserved.</P>

폐기물 매립장 침출수 내의 난분해성 물질 제거를 위한 미생물의 cometabolism 반응성 연구

어성욱 우송대학교 2003 우송대학교 논문집 Vol.8 No.-

For the treatment of non biodegradable COD of landfill leachate, there seemed to be no available biological processes. Aliphatic and aromatic hydrocarbon are revealed as main compounds of NBDCODs. Recent field and laboratory studies have evaluated the potential for aerobic co-metabolism of chlorinated solvents. Different co-metabolic substrates and different methods of application have been tried, including growing indigenous microbes in situ, and injecting into the soil subsurface stains grown in subsurface reactors for their co-metabolic potential. There is much potential for using co-metabolism for treating a broad range of chlorinated aliphatic hydrocarbons. Recirculation wells have potential for adding soluble co-metabolic substrates(i.e. phenol and toluene) into contaminated aquifers, while direct addition of gaseous substrates (i.e. methane and propane) into aquifers also hold promise. Aromatic substrates (phenol and toluene) are best used for treatment of chlorinated ethenes, whereas gaseous co-metabolic substrates are better suited for the treatment of chlorinated methanes and ethanes. In this study, we has focused co-metabolism of Methanotrophs using methane as a co-metabolic substrate. The abiotic and biotic transformation of epoxidess formed during the co-metabolism of chlorinated ethanes by OB3 cultures expressing sMMO was observed. The epoxides of VC and TCE were rapidly transformed abiotically. The cis-1,2 DCE epoxide was transformed by sMMO in the whole cells. No ability to transform the epoxide of t-DCE was observed. The transformation of the c-DCE epoxide was shown to inactivate cells, resulting in decreased transformation.

Characteristics of Several Bacterial Isolates Capable of Degrading Chloroaliphatic Compounds via Hydrolytic Dechlorination

Ji-SookSong,Dong-HunLee,KyoungLee,Chi-KyungKim 한국미생물학회 2003 The journal of microbiology Vol.41 No.4

Haloaliphatic hydrocarbons have been widely used as solvents and ingredients of pesticides and herbicides. However, when these compounds contaminate the environment, they can be very hazardous to animals and humans because of their potential toxicity and carcinogenicity. Therefore, lots of studies have been made for microbial degradation of those pollutant chemicals. In this study, 11 bacterial strains capable of degrading 1,2-dichloroethane (1,2-DCA), 2-chloropropionic acid (2-CPA), 2,3-dichloropropionic acid (2,3-DCPA), and 2-monochloroacetic acid (2-MCA) by hydrolytic dechlorination under aerobic conditions were isolated from wastewaters and rice paddy soil samples. Their morphological and biochemical characteristics and their degradation capabilities of haloaliphatic hydrocarbons were examined. On the basis of the 16S rDNA sequences, 8 different kinds of microbial species, including Pseudomonas plecoglossicida, Xanthobacter flavus, Ralstonia eutropha, were identified. All of the isolated strains can degrade MCA. In particular, strains UE-2 and UE-15 degraded 1,2-DCA, and strain CA-11 degraded 2,3-DCPA, which are hardly degraded by other strains.

휘발성 유기화합물 속도상수의 통계적 분석

김종오,정일현 7개 국립대학교 환경연구논문집 공동발행 위원회 2005 공업기술연구 Vol.5 No.-

The objictives of this study were to investigate degradation rates and to develop a regression model using molecular structure technique for chlorinated aliphatic hydrocarbons (CAHs). DCM(Methypene chloride) and VC(Vinyl chloride) were well degraded by the Methylosinus trichosporium OB3b. From previous investigations, 5 hydrocarbons(DCM, DCA, 1, 1-DCE, 1, 2,-DCE, TCE)except CF(Chloroform) had relatively close rate constants. Regression equations showed a good correlation between the measured and calculated rate constants for CAHs from several results.

아연 광석과 프로판산화 미생물을 이용한 이단 고정상 반응기에서의 염소계 지방족 탄화수소 혼합물 분해 특성

손봉한 ( Bong Han Son ),김남희 ( Nam Hee Kim ),홍광표 ( Kwang Pyo Hong ),윤준기 ( Jun Ki Yun ),이채영 ( Chae Young Lee ),권수열 ( Soo Youl Kwon ),김영 ( Young Kim ) 한국물환경학회 2007 한국물환경학회지 Vol.23 No.5

Methylosinus trichosporium OB3b를 이용한 Chloroform과 Methylene chloride의 분해

양진우,장덕진 명지대학교 대학원 1998 대학원논문집 Vol.2 No.-

Chlorinated aliphatic hydrocarbons (CAHs) represent a major class of pollutants due to their widespread distribution in environments and their toxic and/or carcinogenic properties. Among them, Chloroform (CE) and dichloromethane (DCM) are known to be carcinogens in animals and suspected carcinogens in human. Especially CF is resistant to biodegradation in aquifer environments and persists for extended periods of times. In this paper, Methylosinus tichosporium OB3b was used for the treatments of CF and DCM via cometabolic biotransformations. In varying concentrations from 20μM to 80μM, V_max values were calculated as 5.88∼65nmol/(min·mg protein) and 37.1∼65.3nmol/(min·mg protein), respectively, for resting cells, 256∼947 nmol/(min·mg protein) for CF by non-washed cells.

경안천에서 사슬형 탄화수소 화합물의 농도와 분포

이동석 ( Dong Seok Yi ),박갑성 ( Kap Song Park ) 한국물환경학회 2005 한국물환경학회지 Vol.21 No.5

SIFT-MS를 이용한 공단 영향 주거지역에서의 HAPs 특성 연구

최임조,이동욱,탁경학,조정호 대한환경공학회 2023 대한환경공학회지 Vol.45 No.1

In Yaeum-dong, a residential area near the industrial complex, SIFT-MS, which enables real-time continuous measurement, was used to identify the characteristics of VOCs and odorous substances. When looking at the concentrations by chemical substance group, aliphatic hydrocarbons accounted for a significant portion, showing different characteristics from other large cities. This indicates that the Yaeum-dong area is directly affected by the emission source of the nearby petrochemical complex. As an individual substance, ammonia showed the highest concentration among HAPs. Seasonal HAPs concentrations generally tended to be high in summer and low in winter. When examining concentrations by time of day, it showed characteristics of industrial areas affected by VOCs scattered and volatilized in national industrial complexes. In addition, it was confirmed that the characteristics of urban areas appearing in general metropolitan traffic congestion zones appeared at the same time. Compared with the results of other previous studies, in general, there was no significant difference in concentrations from the results of measurements in residential areas near petrochemical complexes. And it showed a much lower concentrations than the measured value in industrial complex. As a result of CPF analysis for estimating the source of pollutants, most HAPs showed high concentrations in the south wind direction, indicating that the measurement point was affected by the industrial complex. 공단인근 주거지역인 야음동을 대상으로 실시간 연속 측정이 가능한 SIFT-MS를 활용하여 VOCs 및 악취물질의 특성을 파악하였다. 화학물질 그룹별 농도를 살펴봤을 때, 지방족탄화수소류가 상당 부분 차지하고 있어 타 대도시와는 다른 특성을 보였다. 이는 야음동 지역이 인근 석유화학공단의 배출원으로부터 직접적인 영향을 받고 있음을 나타낸다. 개별물질로는 암모니아가 HAPs 중에서 가장 높은 농도를 나타내었다. 계절별 HAPs 농도는 대체적으로 여름에 높고 겨울에 낮아지는 경향을 보였다. 하루 중 시간대별 농도를 살펴봤을 때, 국가산업단지에서 비산 휘발되는 VOCs의 영향을 받는 산업지역의 특성을 보였다. 또한 일반적인 대도시 교통밀집지대에서 나타나는 도시지역의 특성이 동시에 나타나는 것을 확인할 수 있었다. 다른 선행 연구결과와 비교했을 때, 대체적으로 석유화학공단 인근 주거지역의 측정결과와는 큰 농도차이를 보이지 않았다. 그리고 산업단지 내의 측정치 보다는 훨씬 낮은 농도를 나타내었다. 오염물질의 발생원 추정을 위한 CPF 분석 결과, 대부분의 HAPs는 남풍계열의 풍향에서 높은 농도가 나타나 측정지점이 산업단지의 영향을 받고 있음을 알 수 있다.

지방족 탄화수소의 할로겐 유도체 수용액의 광촉매-광분해

전진,정학진,김해진,김삼혁 한국환경과학회 1997 한국환경과학회지 Vol.6 No.1

The rates of photodegradation, reactivities, and mechanisms of photooxidation for the aqueous solution containing with halogen derivatives of aliphatic hydrocarbons have been discussed with respect to the kinds of photocatalysts, concentration of photocatalytic suspensions, strength of radiant power , time of illumination, changes of pH of substrate solution, wavelength of radiation, and pressure of oxygen gas saturated in the solution. These aqueous solutions suspended with 0.5 gL^-1 TiO_2 powder have been photodecomposed in the range of 100 and 93.8% per 1 hour if it is illuminated with wavelength (λ≥ 300nm) produced from Xe-lamp(450W). The photocatalytic abilities have been increased in the order of Fe_2O_3 < CdS < CeO_2 < Y_O2_3 < TiO_2, and rates of photodegradation for the solution have maximum values in the condition of pH 6∼8 and 3 psi-O_2 gL^-1. These rates for the photooxidation per 1 hour were dependent on the size of molecular weight and chemical bonding for organic halogen compounds and the rates of photodegradation were increased in the order of C_2H_5Br < CH_2Br_2 < C_5H_11Cl C_2H_4Cl_2 < trans-C_2H_2Cl_2 < cis-C_2H_2Cl_2 The t_1/2 and t_99% for these solutions were 5∼21 and 40∼90 minutes, respectively, and these values were coincided with initial reaction kinetics(r_0). It was found that reaction of photodegradation has the pseudo first-order kinetics controlled by the amount of h^+_VB diffused from a surface of photocatalysts.