단삼 첨가에 따른 느타리버섯의 생육 특성 및 수량성

이찬중,문지원,유영미,한주연,정종천,공원식,유영진 한국버섯학회 2015 한국버섯학회지 Vol.13 No.3

This study was carried out to investigated optimum mixing ratio of Korean cultivation Salvia miltiorrhiza forproduction of functional oyster mushroom. Mycerial growth was slow at addition of Salvia miltiorrhiza, and significant differenceby increase of Salvia miltiorrhiza. Yields of fruiting body show the highest to 139.5g/850ml of medium which are addition 5g/bottle of Salvia miltiorrhiza but rapid decrease by increase of Salvia miltiorrhiza. Diameter of pileus and thick of stipes were higherat addition Salvia miltiorrhiza than those of the controls. Thick of pileus and length of stipes were the highest at addition 30g, and20g and 30g of Salvia miltiorrhiza. The L value of stipes were the highest at addition 20g and 50g of Salvia miltiorrhiza and theL value of pileus were the highest at addition 30g of Salvia miltiorrhiza, but there was no significant difference in the a-value andthe b-value.

단삼 첨가에 따른 느타리버섯의 생육 특성 및 수량성

이찬중,문지원,유영미,한주연,정종천,공원식,김영국,유영진 한국버섯학회 2015 한국버섯학회지 Vol.13 No.3

This study was carried out to investigated optimum mixing ratio of Korean cultivation Salvia miltiorrhiza for production of functional oyster mushroom. Mycerial growth was slow at addition of Salvia miltiorrhiza, and significant difference by increase of Salvia miltiorrhiza. Yields of fruiting body show the highest to 139.5 g/850 ml of medium which are addition 5 g/ bottle of Salvia miltiorrhiza but rapid decrease by increase of Salvia miltiorrhiza. Diameter of pileus and thick of stipes were higher at addition Salvia miltiorrhiza than those of the controls. Thick of pileus and length of stipes were the highest at addition 30g, and 20 g and 30 g of Salvia miltiorrhiza. The L value of stipes were the highest at addition 20 g and 50 g of Salvia miltiorrhiza and the L value of pileus were the highest at addition 30 g of Salvia miltiorrhiza, but there was no significant difference in the a-value and the b-value.

용매와 추출조건에 따른 단삼 (Salvia mitiorrhiza) 추출물의 항균력

목종수,박욱연,김영목,장동석 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.6

In order to develop a natural food preservative, dried salviae miltiorrhizae radix (Salvia miltiorrhiza) was extracted with several solvents, and then antimicrobial activity was investigated. The optimum extracting condition for the antimicrobial sustance from the sample, minimal inhibitory concentration (MIC) of the extracted substance against microorganisms were also examined. Antimicrobial activity of the initial ethanol extract from the sample was the strongest compared to those of other solvent extracts such as n-hexane, acetone, butanol, methanol and water. the optimum extractingcondition for antimicrobial substance from the sample was shaking extraction for 2 hours at room temperature incase that 10 volumes of absolute ethanol was added to crushed Saliva Miltiorrhiza. The ethanol extract had strong growth inhibition activity against Gram-positive Bacteria (MIC, 3.13-50$\mu\textrm{g}$/ml) such as B. cereus, B, subtilis, L. minocytogenes, S. aureus, Sc. Mutans. Among Grampositive bacteria tested, Bacillus species was the most susceptibile to the extracted substance. The antimicrobial activity of the ethanol extract from the sample was weak to Gram -negative bacteria yeasts, for example MIC for Gram-negative bacteria and yeasts was 0.8mg/ml and 0.4-0.8mg/ml , respectively.

단삼이 당뇨병성 신병증 Rat의 신기능 및 조직학적 변화에 미치는 영향

김영석,이병철,안세영,두호경,안영민,Kim, Young-Seok,Lee, Byung-Cheol,Ahn, Se-Young,Doo, Ho-Kyung,Ahn, Young-Min 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.3

Objective : Diabetic nephropathy is the most common cause of end stage renal disease. AGE, $TGF-{\beta}1$ type IV collagen, and macrophage/monocyte infiltration are the main factors of diabetic nephropathy. We investigated the effects of Salvia miltiorrhiza on renal function and histopathological changes in streptozotocin(STZ)-induced diabetic nephropathy rat model. Methods : Diabetes was induced in male Sprague-Dawley rats(290${\pm}$10g) by injecting STZ(45mg/kg) into the tail vein. Rats were divided into 3 groups(n = 6): normal, control, and salvia. After 8 weeks of administration of Salvia miltiorrhiza extract on the Salvia group, we checked 24 hrs urine, blood biochemistry and renal tissue to evaluate renal function and histopathological changes by examining parameters including albuminuria, BUN, creatinine, cholesterol, LDL, TG, macrophage/monocyte antigen(ED-1), $TGF-{\beta}1$, AGE, and type IV collagen. Results : Salvia miltiorrhiza decreased the amount of 24hrs proteinuria, and inhibited histopathological changes of diabetic nephropathy including the expression and accumulation of various factors which could promote development of diabetic nephropathy. Conclusion : These findings suggest that Salvia miltiorrhiza might protect the renal function and inhibit the development of renal injury by regulating factors including AGE, $TGF-{\beta}1$ Type IV collagen, macrophage and monocyte infiltration. So Salvia miltiorrhiza can be used for diabetic patients to prevent the progression of diabetic nephropathy.

단삼의 재식밀도와 피복재료에 따른 생육 및 수량특성

김영국,여준환,한신희,허목,이영섭,박충범 한국약용작물학회 2013 한국약용작물학회지 Vol.21 No.3

This study was carried out to investigate the variation of growth and yield using different planting densities and vinyl mulching in S. miltiorrhiza cultivation. Top plant growth was observed in the 30×30cm planting density of S. miltiorrhiza;, plant height, leaves and branches were larger than in the other treatments. Root length, root diameter, and supporting roots of underground part were increased as planting density was wide, and dry root weight was increased in 30×30cm. However, yield was highest at 294 kg/10a in the planting density of 30×10cm. The use of 30-31cm white and black vinyl mulching had no significant effect on the plant height of S. miltiorrhiza, as compared to non-mulching. However, the highest volume of leaves and plant weight was observed in plants using 24.7 black vinyl mulching. Dry underground root weight was highest in black vinyl mulching at 21.7 g compared to 17.0 g for non-mulching. Yield per 10a increased by 28% using black vinyl mulching compared to non-mulching. Based on the results of this study, planting density of 30×10cm and black vinyl mulching are the most suitable in the cultivation of S. miltiorrhiza.

A novel photo-biological engineering method for <i>Salvia miltiorrhiza</i>-mediated fabrication of silver nanoparticles using LED lights sources and its effectiveness against <i>Aedes aegypti</i> mosquito larvae and microbial pathogens

Lee, Jeong-Ho,Velmurugan, Palanivel,Park, Jung-Hee,Murugan, Kadarkarai,Lovanh, Nanh,Park, Yool-Jin,Oh, Byung-Taek,Venkatachalam, Perumal,Benelli, Giovanni Elsevier 2018 Physiological and molecular plant pathology Vol.101 No.-

<P><B>Abstract</B></P> <P>In this study, <I>Salvia miltiorrhiza-</I>synthesized Ag nanoparticles (AgNPs) fabricated using sunlight or various LED lights were studied for their biophysical features and evaluated as larvicides against <I>Aedes aegypti</I> mosquitoes and growth inhibitors on different species of microbial pathogens. AgNPs production post-exposure to sunlight or different LED light conditions (i.e. blue, red, green, and white) was confirmed by characteristic surface Plasmon resonance (SPR) at maximum λ of 430, 420, 460, 450, and 460 nm, respectively. Optimization of pH, reducing extract concentration, metal ion concentration and time elapsed from the nano-biosynthesis was achieved. High-resolution transmission electron microscopy (HR-TEM) showed that most AgNPs was spherical, triangular and oval, with average size of 18.5, 28.02, 50.22, 16.26 and 10.12 nm for white, green, red, blue and sunlight, respectively. XRD confirmed the all the obtained AgNPs showed face centered cubic (fcc) crystal lattice. FT-IR analysis of all synthesized AgNPs indicated the involvement of phenol, amine, hydroxyl and amino groups in the reduction of nano-Ag. All tested AgNPs inhibited the growth of <I>Brevibacterium linens</I> (KACC-14346), <I>Propionibacterium acnes</I> (KACC 11946), <I>Staphylococcus aureus</I> (KACC-10768) and <I>Staphylococcus epidermidis.</I> As a general trend, larvicidal assays conducted on dengue and Zika virus vector <I>Aedes aegypti</I> showed that, after 48 h of exposure, the toxicity achieved by sunlight-fabricated AgNPs was slightly higher if compared to AgNPs fabricated using various LED lights. Overall, our research highlighted the importance of abiotic parameters, with special reference to light condition, during green nanosynthesis of antimicrobials and larvicides.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel photo-biological engineering method for <I>Salvia miltiorrhiza-</I>mediated fabrication of silver nanoparticles using LED lights sources was established. </LI> <LI> Engineered biomolecule-capped silver nanoparticles were characterized by using FT-IR, XRD, HR-TEM analysis. </LI> <LI> Green synthesized nanoparticles exhibited enhanced antibacterial activity against microbial pathogens. </LI> <LI> Photo-fabricated metallic silver nanoparticles showed efficient larvicidal activity against dengue and Zika virus vector <I>Aedes aegypti.</I> </LI> </UL> </P>

PTP1B Inhibitory Effect of Abietane Diterpenes Isolated from <i>Salvia miltiorrhiza</i>

Han, Yu Mi,Oh, Hyuncheol,Na, MinKyun,Kim, Beom Seok,Oh, Won Keun,Kim, Bo Yeon,Jeong, Dae Gwin,Ryu, Seong Eon,Sok, Dai-Eun,Ahn, Jong Seog Pharmaceutical Society of Japan 2005 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.28 No.9

<P>Protein tyrosine phosphatase 1B (PTP1B) acts as a negative regulator of insulin signaling, and selective inhibition of PTP1B has served as a potential drug target for the treatment of type 2 diabetes. In the course of screening for PTP1B inhibitory natural products, the MeOH extract of the dried root of <I>Salvia miltiorrhiza</I> B<SMALL>UNGE</SMALL> (Labiatae) was found to exhibit significant inhibitory effect. Bioassay-guided fractionation and purification afforded three related abietane-type diterpene metabolites 1—3. Compounds 1—3 were identified as isotanshinone IIA (1), dihydroisotanshinone I (2), and isocryptotanshinone (3) mainly by analysis of NMR and MS data. Compounds 1—3 non-competitively inhibited PTP1B activity with 50% inhibitory concentration values of 11.4±0.6 μ<SMALL>M</SMALL>, 22.4±0.6 μ<SMALL>M</SMALL> and 56.1±6.3 μ<SMALL>M</SMALL>, respectively.</P>

Antioxidant Effect of Salvia miltiorrhiza

Kang, Hye-Sook,Chung, Hae-Young,Jung, Jee-Hyung,Kang, Sam-Sik,Choi, Jae-Sue The Pharmaceutical Society of Korea 1997 Archives of Pharmacal Research Vol.20 No.5

A strong antioxidant activity, which was measured by the radical scavenging effect on 1, 1-diphenyl-2-picrylhydrazyl(DPPH) radical, was detected in the methanol extract of Salvia miltiorrhiza Bunge (Labiatae). By activity-directed fractionation, compounds 1 and 2 were isolated as antioxidant principles of S. miltiorrhiza. Compounds 1 and 2 were identified as dimethyl lithospermate and 3-(3, 4-dihydroxyphenyl)lactamide, respectively, on the basis of spectral data. The radical scavenging effect of compounds 1 and 2 on DPPH radical exceeded that of L-ascorbic acid which is a well known antioxidant. These two compounds also showed prominent inhibitory activity against free radical generation in dichlorofluorescein (DCF) method and cytoprotective effect against t-BHP in cultured liver cell.

단삼의 수확시기에 따른 생육 특성 및 주요 성분 변이

김영국,안태진,허목,이정훈,이윤지,차선우 한국약용작물학회 2015 한국약용작물학회지 Vol.23 No.5

Background : This study examined the effect of harvesting time on the growth, yield characteristics, and major beneficial components in Salvia miltiorrhiza. Methods and Results : Although plant height, stem diameter and branch length were not affected by harvesting time, the number of stems was highest when harvested in mid October. There were no differences in root length and thickness, however, the rhizome was thicker when it was harvested at the end of October or early November than when it was harvested in early and mid October. The dried root weight also showed a similar pattern. However, there was a statistically significant increase to 408 ㎏ (16%) in the rhizome weight when in late October and a rise to 455 ㎏ (29%) when harvested in early November. Harvest time had little effect on the content of the major component of S. miltiorrhiza. For example, salvianolic acid content rose from 9.42 to 9.64% with later harvest times, and tanshinone ΠA content was tended to be slightly more increased in mid October which S. miltiorrhiza has 0.22% tanshinon ΠA than in early October. Conclusions : According to these results, the optimum harvest time for S. miltiorrhiza is early November when plant or major component yields are hightest. There were no significant harvest time effects on the major beneficial components.

온도에 따른 단삼의 광합성 특성 및 수확시기가 품질에 미치는 영향

서영진,김종수,김선화,김미연,정용진,성기운,정신교 한국식품저장유통학회 2015 한국식품저장유통학회지 Vol.22 No.6

Salvia miltiorrhiza has been used for treating heart and liver disease. In the present study, the influences of temperature on photosynthetical capacity of S. miltiorrhiza under controlled cultivation environment using growth chamber were investigated because of providing information about growth and secondary metabolite synthesis. And effect of harvesting time on growth properties and constituents such as salvianolic acid B, cryptotanshinone, tanshinone I, tanshinone IIA were evaluated. Maximum photosynthesis rate (5.102 μmol CO2/m2/s) and net apparent quantum yield (0.147 μmol CO2/m2/s), stomatal conductance (0.035 mmol/m2/s) and water use efficiency (7.108 μmol CO2/mmol H2O) was highest at 20℃. Results of chlorophyll fluorescence showed that elevated temperature had contributed to reduce a quantum yield and electron flux in photosystem. This result demonstrated that favorable temperature condition was determined at 20℃. Contents of salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA was highest in root sample harvested at 20 March, whereas growth and yield of S. miltiorrhiza had no significant differences with harvesting time. Therefore, this study shows that temperature play an important role in photosynthetic activity and harvesting time have influence upon accumulation of constituents in root of S. miltiorrhiza.