소 유방염 유래 Staphylococcus aureus의 AFLP 지문분석

김연수,김상균,황의경,Kim, Yeon-soo,Kim, Sang-kyun,Hwang, Eui-kyung 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.2

Amplified fragment length polymorphism(AFLP) technique is based on the polymorphism detection through selective PCR amplification of restriction fragments from digested genomic DNA and thus includes the procedures of the total DNA digestion by endonucleases, ligation of adapters to the ends of the fragments, and following the selective amplification of the restricted DNA fragments. This study were aimed to : (1) determine the genetic variability of S aureus strains, (2) estimate genetic diversity within and among these strains, (3) compare phylogenetic relationships among these strains as genetic markers using AFLP techniques. Genomic DNA was digested with a particular combination of three restriction enzymes with specific recognition sites and the DNA fragments were ligated to restriction specific adapters and amplified using the selective primer combinations. In the S aureus strain, the number of scorable AFLP bands detected per each primer combination varied from 29 to 102, with an average of 61.59 using 27 primer combinations. A total of 1,663 markers were generated, 904 bands of which were polymorphic, showing a 33.48% level of polymorphism with these primer combinations. Among the primer combinations, E02/T02, E02/T03, E04/H02, E02/T01 and E04/H03 primer combinations showed a high level of polymorphism with 0.78, 0.76, 0.74, 0.71 and 0.70, respectively. But T03/H01, E01/T02 and E01/T03 primer combinations showed a low level of polymorphism with 0.38, 0.37 and 0.15, respectively, Therefore, the former primer combinations will be the most effective for AFLP analysis of S aureus. In SA1 sub-types the level of polymorphism of S aureus KCTC 1927 was similar to that of S aureus CU 01(0.825) and higher than those of other strains such as S aureus CU 02 (0.715), S aureus KCTC 2199(0.625), S aureus KCTC 1916(0.607) and S aureus KCTC 1621 (0.553). In SA2 sub-types the level of polymorphism of S aureus CU 07 was similar to that of S aureus CU 08(0.935) and higher than those of both S aureus CU 04(0.883) and S aureus CU 05(0.883) and lower than those of S aureus CU 03(0.583). In SA3 subtypes the level of polymorphism of S aureus CU 11 was similar to that of S aureus CU 12(0.913) and lower than that of S aureus CU 15(0.623). The results proved that AFLP marker analysis of S aureus strain could be used to study the epidemiology of mastitis and in addition, common genotype in geographic region could be useful for the development of an effective vaccine or DNA marker for easy diagnosis of mastitis caused by S aureus infection.

황색포도구균혈증의 임상적 고찰

홍화정(Hwa Jeong Hong),이정환(Chung Hwan Lee),박종오(Chong Oh Park),정일우(Il Woo Jung),이승호(Seung Ho Lee),고경식(Kyung Sik Ko),김구엽(Koo Yeop Kim),서환조(Hwan Jo Suh) 대한내과학회 1997 대한내과학회지 Vol.53 No.3

Objective: Staphylococcus aureus has persisted and is now resurging as an important hospital and community pathogen. Nosocomial infection caused by methicillin-resistant S.aureus(MRSA) is a major problem which may be connected with heavy or prolonged use of antibiotics S.aureus bacteremia caused acute complications, which occasionally resulted in death, and infectious/suppurative complications, which necessitated prolonged antibiotic therapy, sometimes in conjunction with surgery. Therefore, S.aureus bacteremia is a serious medical problem in association with high morbidity and mortality. Methods: 130 patients with S.aureus bacteremia who were admitted in the Kyung Hee University Hospital from January, 1991 to December, 1994 were analyzed retrospectively. We compared the clinical and laboratory characteristics, and antibiotics resistances between MRSA and MSSA bacteremia and also we evaluated risk factors that contribute to fatal outcome in patients with S.aureus bacteremia. Results : 1) of 130cases, 80 were male and 50 were female. The mean age was 44.5±25.1 years. 2) 84(65%) of S.aureus bacteremia were nosocomial and 46(35%) were community-acpuired. The percentage of MRSA stains studied was 55%(71/130) and The percentage of MRSA bacteremia in hospital-acpuired and community-accquired S. aureus bacteremia were 64% (54/84) and 36%(17/46), respectively. Sources of bacteremia were uncertain in 85(65%) with intravascular catheter(20%) and skin wound sites (8%) being the most common sources in remainder(35%) 3) 110(85%) of 130 patients had one or more underlying diseases. Common underlying dieases were cerebrovascular disease(33%), malignancy(17%), Diabetes mellitus(15%), chronic renal failure(8%) and liver cirrhosis(6%). 4) Acute complications occurred in 35 patients and were fatal in 21 5) The risk factors associated with MRSA bacteremia were various severe underlying diseases, vairous invasive procedures, IV catheter-associated infection, hypoalbuminemia, previous use of antibiotics, male sex and old age. 6) The Case fatality rate for patients with S. aureus bacteremia was 18% and those for patients with MRSA and MSSA bactermia were 20% and 12%, respectively. The risk factors that contribute to the increment of mortality rate in patients with S. aureus bacteremia were acute complication, low serum level of total protein, hypoalbuminemia, various invasive procedures and IV catheter-associated infection, 7) In the antibiotic sensitivity test S. aureus was resistant to penicillin in 98.5%, ofloxacin in 73%, cefotaxime in 67%, erythromycin in 58%, aztreonam in 56%, clindamycin in 52%, vancomycin in 0%. 8) In the multiple antibiotic resistance of S. aureus, 43(68%) of MRSA was resistant to more than 10 antibotics, revealing multiply resistant nature of strains, While all but one MSSA was resistant to 1 to 4 antibiotics, one revealing resistance to 8 antibiotics. Conclusion: S. aureus bacteremia is a cause of considerable morbidity and mortality in hospitalized patients who especially, exposed to various risk factors. MRSA revealed higher resistance rate to most antibiotics tested and more marked multiply resistant nature than MSSA. But there was no significant difference in case fatality rate between patients with MRSA and MSSA bacteremia.

항생제(抗生劑) 내성(耐性) 화농균(化膿菌)의 생육(生育)을 억제(抑制)하는 한약재(韓藥材) 탐색(探索)에 관(關)한 硏究(연구)

박원영,서운교,정지천,Park, Won-Young,Seo, Woon-Gyo,Jeong, Ji-Cheon 대한한방내과학회 1998 大韓韓方內科學會誌 Vol.19 No.2

The various oriental herbal medicines, which have usually been used for treatment of reducing fever, purging intense heat and detoxication, were screened to determine the antibacterial activity and the minimal inhibitory concentration against antibiotics-resistant Staphylococcus aureus. The results obtained were as follows: 1. Among the 23 oriental medicines tested, the water-soluble extract of Coptis japonica showed very excellent antibacterial activity against antibiotics-sensitive and -resistant S. aureus. A little activity was found in the extracts of Scutellaria baicalensis, Eriobotrya japonica and Prunus mume against antibiotics-sensitive S. aureus and in that extracts of P. mume, Schizandra chinensis and S. baicalensis against antibiotics-resistant S. aureus. When C japonica was used, the high antibacterial activity was shown against Bacillus subtilis and other extracts showed a little or no antibacterial activity against B. subtilis and E. coli as a control. 2. The highest antibacterial activities against antibiotics-resistant S. aureus were found in the ethanol-soluble extract of C japonica. Other ethanol-soluble extracts of S, baicalensis, S. chinensis and P. mume showed a little activity against both antibiotics-sensitive and antibiotics-resistant S. aureus. 3. Among the medicinal herbs tested, the water- and ethanol extracts of C. japonica showed very excellent antibacterial activity against the pathogenic S. aureus and both Escherichia coli and Bacillus subtilis used as a control. 4. When the water-soluble extracts of C. japonica and S. baicalensis, minimal inhibitory concentrations (MICs) against antibiotics-sensitive S. aureus were $20mg/m{\ell}\;and\;30mg/m{\ell}$, respectively. The MICs of the ethanol-soluble extracts of C. japonica and P. scabriosaefolia were $7.5mg/m{\ell}\;and\;12mg/m{\ell}$, respectively. 5. For the MICs against antibiotics-resistant S. aureus, the water-soluble extracts of C. japonica and P. mume showed $15mg/m{\ell}\;and\;10mg/m{\ell}$, respectively. The MICs of the ethanol-soluble extracts of C. japonica and P. mume were $13mg/m{\ell}\;and\;20mg/m{\ell}$, respectively. As a result, the highest antibacterial activity was found in both water- and ethanol-soluble extracts of C. japonica against antibiotics-resistant S. aureus. And the water- and ethanol-soluble extracts of S. baicalensis, S. chinensis and P. mume showed comparatively high antibacterial activities against both antibiotics-sensitive and -resistant S. aureus.

Thymol attenuates the worsening of atopic dermatitis induced by <i>Staphylococcus aureus</i> membrane vesicles

Kwon, Hyo Il,Jeong, Na Hee,Jun, So Hyun,Son, Joo Hee,Kim, Shukho,Jeon, Hyejin,Kang, Sun Chul,Kim, Sang Hyun,Lee, Je Chul Elsevier 2018 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.59 No.-

<P><B>Abstract</B></P> <P> <I>Staphylococcus aureus</I> membrane vesicles (MVs) aggravate atopic dermatitis (AD) through the delivery of bacterial effector molecules to host cells and the stimulation of inflammatory responses. This study investigated the inhibitory effect of thymol, a phenolic monoterpene found in essential oils derived from plants, on the worsening of AD induced by <I>S. aureus</I> MVs both <I>in vitro</I> and <I>in vivo</I>. The sub-minimal inhibitory concentrations of thymol disrupted <I>S. aureus</I> MVs. Intact <I>S. aureus</I> MVs induced the expression of pro-inflammatory cytokine (interleukin (IL)-1β, IL-6, and tumor necrosis factor-α) and chemokine (IL-8 and monocyte chemoattractant protein-1) genes in cultured keratinocytes, whereas thymol-treated <I>S. aureus</I> MVs did not stimulate the expression of these genes. Topical application of thymol-treated <I>S. aureus</I> MVs or treatment with thymol after intact <I>S. aureus</I> MVs to AD-like skin lesions diminished the pathology of AD. This included decreases in epidermal/dermal thickness and infiltration of eosinophils/mast cells, and inhibited expression of pro-inflammatory cytokine and chemokine genes in mouse AD model. Moreover, thymol significantly suppressed the Th1, Th2, and Th17-mediated inflammatory responses in AD-like skin lesions induced by <I>S. aureus</I> MVs, and reduced the serum levels of immunoglobulin (Ig) G2a, mite-specific IgE, and total IgE. In summary, thymol disrupts <I>S. aureus</I> MVs and suppresses inflammatory responses in AD-like skin lesions aggravated by <I>S. aureus</I> MVs. Our results suggest that thymol is a possible candidate for the management of AD aggravation induced by <I>S. aureus</I> colonization or infection in the lesions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The subMICs of thymol disrupt <I>S. aureus</I> membrane vesicles (MVs). </LI> <LI> Thymol-treated <I>S. aureus</I> MVs do not induce inflammatory responses in keratinocytes. </LI> <LI> Thymol inhibits worsening of AD-like lesions induced by <I>S. aureus</I> MVs. </LI> </UL> </P>

마크로라이드-린코사마이드-스트렙토그라민 B(MLS)계 항생물질에 대한 유도 내성

권애란(Ae Ran Kwon),최성숙(Sung Sook Choi),김숙경(Sook Kyung Kim),정영자(Young Ja Chung),최응칠(Eung Chil Choi),김병각(Byoung Kak Kim) 대한약학회 1994 약학회지 Vol.38 No.3

Forty nine clinical isolates of S. aureus showing resistance to erythromycin(EM) were selected from 83 strains isolated recently in Korea. Fourteen strains of S. aureus showing inducible resistance to MLS antibiotics were selected by disc agar diffusion method. Colony hydridization was executed using two MLS inducible resistance genes, ermA and ermC, identified previously from S. aureus as probes. S. aureus 375 and S. aureus 507 whose genes were not homologous to those probes were finally selected. It was confirmed that the resistance genes of S. aureus 375 and S. aureus 507 had no homology with those probes in southern hybridization test using ermA, ermC and ermAM as probes. It was determined that S. aureus 375 had a plasmid whose size was about 35 kb. To know if the plasmid may have the genes related to inducible resistance to MLS antibiotics, it was attempted to transform Bacillus subtillis BR151 and S. aureus RN4220 with the plasmid isolated from S. aureus 375. It was shown that the gene related to inducible resistance to MLS antibiotics did not exist in this plasmid. These results indicate that two clinical isolates of S. aureus showing inducible resistance to MLS antibiotics have novel genes that have no homology with MLS resistance genes identified so far. It is assumed that these genes may exist in chromosomal DNA.

서울대학교병원 환자 혈액에서 분리된 Staphylococcus aureus, E. coli, Klebsiella pneumoniae 및 Pseudomonas aeruginosa의 항균제감수성

김의종,허미나,한규섭,박명희 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.1

목적 : 임상미생물검사중에서 환자의 치료에 가장 도움을 줄 수 있는 검사 중의 하나는 혈액배양검사라고 할 수 있다. 이 결과를 바탕으로 하여 항생제를 투여해야 하지만 항생제감수성검사가 나오기 전까지는 경험적으로 항생제를 선택하여 사용하게 된다. 그리나 최근 병원균의 항생제에 대한 내성률이 증가하고 있기 때문에, 병원바다 특성에 맞는 경험적 선택기준을 정해야 한다. 본 연구에서는 서울대학교병원 환자의 혈액예서 흔히 분리되는 병원균의 항생제감수성양상을 파악함으로써 균혈중 환자를 치료할 때 항생제를 선택할 수 있는 기본자료를 마련하고자 하였다. 방법 : 1996년 4월부터 10월까지 서을대학교병원에 내원한 환자의 혈액으로부터 분리된 S. aureus, E. coli, K. pneumoniae 및 P. aeruginosa의 수종의 항근제에 대한 감수성양상을 파악하기 위하여 microdilution broth법으로 최소억제농도를 측정하였다. 대상균주로는 기간중 혈액에서 분리된 methicillin-susceptible S. aureus (MSSA) 24 주, methicillin-resistant S. aureus (MRSA) 18 주, E. coli 87 주, K. pneumoniae 53 주, 및 P. aeruginosa 19 주로써, 총 201 균주를 대상으로 하였다. 동일한 환자에서 중복분리된 균주는 제외하였다. 결과 : S. aureus에 의한 균혈증환자 42명 중 oxacillin에 대한 내성(MRSA) 감염증은 18 명으로서 42.9%에 해당하였다. MRSA는 모두 imipenem, gentamicin, ofloxacin에 대해 내성이었으나, vancomycin이나 teicoplanin에 대한 내성 균주는 발견되지 않았다. MSSA중 29%가 gentamicin에 대해 내성이었으나 이 균주들은 모두 cefazoline과 imipenem에 대해 감수성이었다. 지역사회에서 S. aureus에 의해 균혈증을 일으킨 환자 중에서는 MRSA에 의한 감염증이 한 명도 없었다. E. coli의 61%rk ampicillin에 대해 내성이었으며, cefazoline에 대한 내성률은 14%이었다. K. pneumoniae는 cefazoline에 대하여 23%가 내성이었다. P. aeruginosa의 piperacillin 에 대한 내성은 21%이었으며, ticarcillin/clavulanic acid에 대한 내성률은 42%이었다. P. aeruginosa의 imipenem 에 대한 감수성률은 90%이어으며, gentamicin과 amikacin에 대한 감수성률은 각각 84%와 95%이었다. 결론 : S. aureus에 의한 균혈증 환자 중 지역사회감염으로 생각되면 경구용 베타락탐제와 gentamicin을 투여하며, 병원감염이 의심되면 세균이 분리되기 전까지 우선 vancornycin으로 치료를 시작하는 것이 좋을 것으로 생각한다. 요로감염증, 담도감염 또는 위장관감염증이 없는 폐혈증 환자에는 우리나라에서는 이 경우 ampicillin 대신에 1 세대 세팔로스포린항생제를 투여해야 할 것으로 생각한다. P. aeruginosa에 의한 패혈증인 경우 세계보건기구에서 권장하는 바와는 다르게 세균검사 결과가 나오기 전에는 tobramycin과 piperacillin으로 치료를 시작하는 것이 좋을 것으로 생각한다. Background. One of the most informative tests of clinical microbiology laboratory is the blood culture. Antibiotics should be administrated in the basis of culture report, but empirically selected until susceptibility results are obtained. Because the resistance rate is increasing recently, physicians should have their o m guideline of antibiotic treatment. In this study, the data of antibiotic susceptibility of the common causative pathogens isolated from blood of patients were analyzed. Methods ' Minimal inhibitory concentrations of S. uureus, E colz. K. pneumonzae, and P. uerugznosu isolated from blood of patients in Seoul National University Hospital were measured by microdilurion broth method. The isolates tested were 24 strains of methicillin-susceptible S. auureus (MSSA), 18 MRSA. 87 E. coli, 53 K pneuimoniae, and 19 P. aeruginosa. Results : The proportion of MRSA was 42.9% among the patients with S. aureus bacteremia. All of MRSA strains were resistant to imipenem, gentamicin. and ofloxacin. but susceptible to vancomycin and teicoplanin. Twenty njne percent of MSSA strains were resistant to gentamicin. but all of these strains were susceptible to cefazoline and imipenem. There was no MRSA infection in the patients with community-acquired bacteremia. The resistance rates of E. coli against ampicillin and cefazoline were 61% and 14%, respectively. Twenty three percent of R pneumoniae strains were resistant to cefazoline. In the case of P. aeruginosa, the resistance rate of pipera was 21%, but that of ticarcwclavulanic acid was 42%. The susceptibility rates of P. aeruginosa against hipenem, gentamicin, and amikacin were 90%, 84% and 90%, respectively. Conclusion : Oral 0-lactams and gentamicin are recommended for the treatment of patients with community-acquired bacteremia due to S. aureus. But the patients with nosocomial infection by S. aureus m y be empirically treated with vancomycin. If the sources of bacteremia can be predicted as urinary tract, biliary tract or intestinal infections, the first generation of cephalosporin is recommended instead of ampicillin. The initial therapy for bacteremia due to P. aeruginosa is the combination of piperacillin and tobramycin until the susceptibility result is reported.

황색 포도상구균 균혈증의 임상적 의의

박승철,김민자,정문기,김우주,정희진 대한감염학회 1994 감염 Vol.26 No.3

1987년 7월부터 1993년 6월까지 고대 구로 병원에 입원하여 혈액 배양검사를 통하여 S. aureus 균혈증으로 확인된 102예의 환자들을 대상으로 사망률 및 임상상을 관찰 분석하였다. 1)전체적인 S. aureus 균혈증이 사망률은 31%였으며 MRSA 균혈증으로 인한 사망률은 55%로서 MSSA의 20%에 비해 상대적으로 높은 사망률을 보였다. 2)MRSA 균혈증에 이환될 위험을 높이는 요인들은 심각한 기저질환, 항균제 또는 스테로이드 사용의 기왕력, 각종의 침습적 조작, 저알부민혈증 등이었다. 3)S. aureus 균혈증으로 인한 사망 위험을 증가시키는 요인들은 고혈당, 저알부민혈증, 각종의 침습적조작, 항균제 또는 스테로이드 사용의 기왕력, 병원내감염, 호흡기졔 감염, MRSA 균혈증 등이었다. 4)다중회귀분석을 시행한 결과 저알부민혈증, MRSA 균혈증, 고혈당등이 S. aureus 균혈증으로 인한 사망률 증가와 밀접한 관계가 있음을 알수 있었다. Background: Since the introduction of antimicrobial agents, considerable changes have been taken place regarding the microbiologic patterns of bactermia. Gram negative bacteriae have largely been replaced in this role with Staphylococci. Especially when caused by methicillin-resistant Staphylococcus aureus(MRSA) strains, S. aureus bacteremia is serious medical problem in association with high morbidity and mortality. Methods: In this retrospective study, we analysed 102 patients with S. aureus bacteremia who were admitted in the Korea University Guro Hospital over year period, to evaluate risk factors of S. aureus bacteremia related to death and compare clinical features of MRSA bacteremia with those of MSSA bacteremia. Results: 1)The case fatality rate for patients with S. aureus bacteremia was 31%, and those for MRSA and MSSA bateremia were 55% and 20%, respectively. 2)The risk factors associated with MRSA bacteremia were the serious underlying diseases, previous use of antibiotics, steroids, various invasive procedures, and low serum albumin levels. 3)The risk factors that attributed to the increment of mortality rate in patients with S. aureus bacteremia were the high blood glucose, low serum albumin, various invasive procedures, hospital-acquired infection. respiratory tract infection as a primary focus, MRSA infection. Conclusion: Risk facors of fatal outcome in patients with S. aureus bacteremia were, low serum albumin, MRSA infection, high blood glucose by multivariate analysis in our study. In addition, MRSA bacteria was more frequent in patients with hospital-acquired infection, various invasive procedures, previous use of antibiotics, steroids and low serum albumin.

훈증소독제, Fumagari OPP®의 Staphylococcus aureus에 대한 살균효과

차춘남,이후장,박은기,최현주,김용팔,유창열,김석 한국식품위생안전성학회 2013 한국식품위생안전성학회지 Vol.28 No.4

This study was performed to evaluate the bactericidal efficacy of Fumagari OPP®, fumigation disinfectant,containing 20% ortho-phenylphenol against Staphylococcus aureus (S. aureus). In this research, efficacy test of fumigant against S. aureus was carried out according to French standard NF T 72-281. S. aureus working culture suspension number (N value), all of the colony numbers on the carriers exposed with the fumigant (n1, n2, and n3), the number of bacterial test suspentions by pour plate method (N1), the number of bacterial test suspentions by filter membrane method (N2) and the mean number of bacteria recovered on the control-carriers (T value) were obtained from the preliminary test. In addition, the reduction number of S. aureus exposed with the fumigant (d value)was calculated using T value, the mean number of bacteria in recovery solution (n'1) and the mean number of bacteria on carriers plated in agar (n'2). N value was 4.0 × 108CFU/mL, and n1, n2, and n3 were higher than 0.5N1, 0.5N2 and 0.5N1, respectively. Additionally, T value was 3.4 × 106 CFU/mL. In the bactericidal effect of the fumigant, the d value was 6.43 logCFU/mL. According to the French standard for the fumigant, the d value for the effective bactericidal fumigant should be over than 5 logCFU/mL. The results indicated that Fumagari OPP® had an effective bactericidal activity against S. aureus, then the fumigant can be applied to disinfect food materials and kitchen appliances contaminated with pathogenic bacteria. 본 연구는 20% ortho-phenylphenol을 함유한 훈증소독제, Fumagari OPP®의 Staphylococcus aureus (S. aureus)에대한 살균효과를 평가하기 위해, French standard NF T72-281에 따라 수행하였다. 배양 현탁액 중 S. aureus의 균수 (N 값), 훈증소독제에 노출된 각 담체의 균수 (n1, n2,n3), 평판배지법에 의한 시험균주 현탁액 중 균수 (N1), 여과법에 의한 시험균주 현탁액 중 균수 (N2), 그리고 대조담체의 회복 균수의 평균값 (T 값)을 예비실험을 통해 구하였다. 또한, 훈증소독제에 노출된 S. aureus의 감소 균수 (d 값)는, T 값, 회복액 중 균수의 평균값 (n'1) 그리고 배지의담체에서 증식한 균수의 평균값 (n'2) 등을 이용하여 산출하였다. N 값은 4.0 × 108 CFU/mL이었으며, n1, n2, n3은각각 0.5N1, 0.5N2, 0.5N1 보다 높게 나타났다. 그리고 T값은 3.4 × 106CFU/mL이었다. 훈증소독제의 살균효과에있어서, d 값은 6.43 logCFU/mL이었다. 훈증소독제에 대한 프랑스 기준에 따르면, 효과적인 살균력을 갖는 훈증소독제의 d 값이 5 logCFU/mL 이상이어야 하는 것으로규정하고 있다. 본 연구의 결과로부터, Fumagari OPP®는S. aureus에 대해 높은 살균효과를 갖는 것으로 나타나, 병원성 세균으로 오염된 식품재료와 주방기기의 소독에 적용할 수 있을 것으로 사료된다.

세안탕(洗眼湯)이 Staphylococcus species(S. aureus, S. epidermidis)의 배양일에 따른 항균효과의 지속성에 관한 연구

전오도,서형식,Jeon, O-Do,Seo, Hyeong-Sik 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.2

Objectives : This experimental study was performed to investigate the continuous anti-bacterial potency of Sean-tang on cultivation of Staphylococcus species(S. aureus, S. epidermidis) that induce eye disease. Methods : Minimal inhibitory concentration(MIC) was measured by dropping to 50${\mu}$l diluted Sean-tang(100%, 10%,1%, 0.1%) on S. aureus, S. epidermidis that were cultivated from 2 to 6 days. 1. Anti-bacterial potency was measured by the size of inhibition zone with change of volume(20${\mu}$l, 30${\mu}$l, 40${\mu}$l, 50${\mu}$l). Results : 1. Anti-bacterial potency of Sean-tang on S. aureus was appeared in 100% and increased on 6 days. Anti-bacterial potency with change of volume was increased in propotion to increase volume. Anti-bacterial potency of Sean-tang on S. aureus was appeared continuous. 2. Anti-bacterial potency of S. epidermidis was appeared in 100%, 10%, 1% on 2 days and in100%, 10% on 6 days. In 100% Sean-tang, Anti-bacterial potency of 6 days was increased, in 10%, 1%, Anti-bacterial potency of 2 days was increased. Anti-bacterial potency with change of volume was increased inpropotion to increase volume and increased on 6 days, but bacteria was increased. Anti-bacterial potency Sean-tang on S. epidermidis wasn't appeared continuous. Conclusions : Anti-bacterial potency of Sean-tang on cultivation of S. aureus was showed continuous, but on cultivation of S. epidermidis was not showed continuous.

탕포산(湯泡散)이 Staphylococcus species(S. aureus, S. epidermidis)의 배양일에 따른 항균효과의 지속성에 관한 연구

서형식,Seo, Hyeong-Sik 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.2

Objectives : This experimental study was performed to investigate the continuous anti-bacterial potency of Tangpo-san on cultivation of Staphylococcus species(S. aureus, S. epidermidis)that induce eye disease. Methods : Minimal inhibitory concentration(MIC) was measured by dropping to 50 ${\mu}$l diluted Tangpo-san(100%, 10%, 1%, 0.1%) on S. aureus, S. epidermidis that were cultivated from 2 to 6 days. Anti-bacterial potency was measured by the size of inhibition zone with change of volume(20 ${\mu}$l,30 ${\mu}$l,40 ${\mu}$l,50 ${\mu}$l). Results : 1. Anti-bacterial potency of Tanpo-san on S. aureus was not appeared all samples. Anti-bacterial potency with change of volume was increased in propotion to increase volume, and the Anti-bacterial potency of 2 days was equal to 6 days. Anti-bacterial potency of Tangpo-san on S. aureus was appeared continuous. 2. Anti-bacterial potency of Tangpo-san on S. epidermidis was appeared in 100%, 10% on 2 and 6 days, and the Anti-bacterial potency of 6 days was decreased. In 2 days, Anti-bacterial potency was appeared 40 and 50u1, in 6 days, Anti-bacterial potency was appeared all samples. Anti-bacterial potency with change of volume was increased in propotion to increase volume and increased on 6 days, but bacteria was increased. Anti-bacterial potency of Tangpo-san on S. epidermidis wasn't appeared continuous. Conclusions : Anti-bacterial potency of Tangpo-san on cultivation of S. aureus showed continuous, but on cultivation of S. epidermidis was not showed continuous.