시약의 성상에 따른 시약 관리 시스템

최형욱, 이종원, 정회경 배재대학교 공학연구소 2016 공학논문집 Vol.18 No.1

Recently for the safe and efficient management of reagents, Smart Reagent Cabinet Research is underway. The developed Smart Reagent Cabinet system manage’s the reagent information, like the reagent input, shipment record, information of the reagent in the cabinet, reagent cabinet information among other informations. However, there are not enough systems to manage and classifying the reagents and the properties of each reagent. In this paper, we design a reagent management system, applying 'Database Modeling' which classifies the existing reagents in Smart Reagent Cabinet System according to the properties and characteristics of the reagents. Therefore, it is considered that safe reagent management system will help manage the characteristics of each reagent separately. 최근 시약들을 안전하고 효율적으로 관리하기 위한 스마트 시약장 연구가 진행 중에 있다. 개발된 스마트 시약장은 시약 정보, 시약 입·출고 기록, 시약장 정보 등으로 나누어 시약들의 정보를 관리하고 있다. 하지만 시약들의 성상을 고려하여 관리하는 부분은 미비한 실정이다. 본 논문에서는 기존 스마트 시약장 시스템에 시약들이 가지는 성상과 특성에 맞춰 분류하는 데이터베이스 모델링(Database Modeling)을 적용한 시약 관리 시스템을 설계한다. 이에 따라 시약들이 가지는 각각의 특성에 맞는 관리 방법을 통해 안전한 시약 관리가 이루어질 것으로 사료된다.

수종의 PT와 APTT 검사용 시약의 비교

이영경,박성섭,이진숙,이영준,함천경,이유경,조한익 대한임상병리학회 1996 대한임상병리학회지 Vol.16 No.4

Quality Determination of Grignard Reagent using Raman Spectroscopy Combine with Multivariate Analysis

( Rahul Joshi ),( Ritu Joshi ),( Santosh Lohumi ),( Byoung-kwan Cho ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

The Grignard reagent is an organometallic reagent with a general formula RMgX where X is a halogen and R is an alkyl or aryl group (on the basis of benzene ring) obtained by the reaction of halogenoalkane in presence of magnesium and diethyl ether. It is a key reagent which is required in the formation of new C-C bond in the chemical compounds. Further, the Grignard reagent is the active pharmaceutical ingredient in API and thus played a very important role in drug formation in the pharmaceutical industries. Since during the preparation of the reagent, some impurities were developed which is generally undesirable and due to this, the quality of the reagent is readily affected. Analytical techniques like HPLC and Gas chromatography were generally used for determining the quality attributes of the reagent. Thus, there is a need to develop a technique which will overcome all these limitations for the quality determination of the Grignard reagent. Raman spectroscopy is considered to be the most promising analytical technique in the sector of the food industry and additionally used in the chemical analysis of the substances. So, the objective of this study is to demonstrate Raman spectroscopy as a rapid, noninvasive, and high-throughput system for measuring the following parameters within the spectral range from 1800-900cm-1. A net analyte signal (NAS)- based methodology, named HLA/GO (hybrid linear analysis) and has been used as a data analysis method for the acquired spectral data. For making the standard, samples were diluted in toluene within the concentration range from (0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25 and 30%) and 10 replicates has been selected for each group during the acquisition of the spectral data. For the reference analysis, titration method will be used for the impurity identification. The whole process for the experiment i.e the standard sample preparation and the titration is going to be done inside the chemical fume hood in order to avoid the risk during the experiment causes due to the toxicity of the used reagents. Thus, the study suggests that Raman spectroscopy coupled with HLA/GO method can be used to rapidly determine the active content in the Grignard reagent and identity confirmation non-destructively and could be used as an alternative technique and the developed method eliminates any safety concern due to direct exposure to the Grignard reagent.

혈액형검사용 anti-D 시약 부족 시 비상 계획: 희석된 anti-D 시약 사용의 유효성 확인

김태신,정연호,유재형,김소율,남형주,신수 대한수혈학회 2024 大韓輸血學會誌 Vol.35 No.2

Background: In early 2024, there was an issue with the supply of the anti-D reagent for blood typing. This reduced the anti-D reagent in our laboratory below the minimum stock level. We validated the appropriateness of using diluted anti-D reagents as a contingency plan in the event of an anti-D reagent shortage. Methods: A total of eight dilutions, ranging from 2X to 256X, were prepared by serial dilution of the low-protein monoclonal anti-D reagent. The original anti-D reagent and the eight anti-D dilutions were used to perform RhD typing by the tube and plate methods. To further evaluate the reactivity and stability of the 8-fold diluted anti-D reagent, RhD typing was performed on internal quality control red blood cells and RhD-positive patient specimens for 30 days. Results: The maximum dilution that gave the same results as the original anti-D reagent in both the tube and plate methods was 8X. The 8X anti-D dilution was tested against internal quality control red blood cells and patient specimens. It showed the same result as the original anti-D reagent, with reactivity remaining constant over 30 days. Conclusion: We have confirmed the appropriateness of using a diluted low-protein monoclonal anti-D reagent for RhD typing. Therefore, we suggest that the diluted anti-D method can be considered for priority use in emergencies when the anti-D reagent is in short supply. Although 8X is suggested as an appropriate dilution factor in this study, this may vary depending on the type of product used in each laboratory and the laboratory conditions. (Korean J Blood Transfus 2024;35:106-112)

Glucose oxidase-oxygen rate method를 利用한 自動生化學分析裝備용 Glucose 試藥開發에 관한 硏究

마경란,김영기,이갑노,백승룡 고려대학교 의과대학 1992 고려대 의대 잡지 Vol.29 No.1

The detection methods for glucose in clinical pathology have been developed and currently most laboratories measure the glucose level by enzymatic procedures in automated analytical instrument to increase analytical specificity. The most frequently used procedures are glucose oxidase and hexokinase reactions. Beckman Chemistry Autoanalyzer(ASTRA-8® and Glucose Analyzer-2®)adopted glucose oxidase-oxygen rate method employing Oxygen Electrode in glucose module. The Oxygen Electrode senses oxygen concentration and solid state-circuits in the instrument system electronics determine the rate of oxygen consumption and calculate glucose concentration for each sample. Reagent for glucose analysis in ASTRA module is composed of glucose oxidase, alcohol, iodide and buffer solution. Because all reagents used in Korea have been imported from the Beckman or other foreign companies, in this study the author developed and evaluated the new reagent for glucose oxidase-oxygen rate method. The reagent is composed of glucose oxidase, mercuric iodide, propanol and phosphate buffer(pH 6.0). In the evaluation of the newly developed reagent as useful as clinical analytical reagent, the accuracy, precision and linearity tests were performed and compared the results with those obtained by Beckman original method. The results were summarized as follows: 1. The imprecision of newly developed reagent was 0.59 to 2.80% (CV) in the range of 40 to 200 mg/dL. 2. Day-to-day variance for one month was 1.25 to 2.20% in the range of 40 to 400 mg/dL. 3. The accuracy by patient comparison study was excellent with high correlation coefficient (r=0.9994) with the result by original reagent. 4. The recovery rate was in the range of 94.7 to 101.2%. 5. The linearity measured by aqueous standards was good in the range of 0 to 450 mg/dL. 6. The newly developed reagent was stable for 30 days during evaluation period after mixing the reagent at room temperature. In conclusion, the new reagent is satisfactory in precision, accuracy and linearity and may be used for routine laboratory test.

자동화학검사용 영동제약 액상시약의 평가

배숙영,김석수,박영남,김영기 대한임상검사정도관리학회 2002 臨床檢査와 精度管理 Vol.24 No.1

자동화학검사용 인화 Liqchem® 액상시약의 정밀도, 직선성 및 상관성 평가

조현정,기창석,이기오,옥윤주,김소미,이수연,김종원 대한임상검사정도관리학회 2001 臨床檢査와 精度管理 Vol.23 No.2

배경: 임상화학검사는 임상병리 검사실에서 가장 빈번하게 시해오디는 검사로 자동화학분석기의 발전과 더불어 비약적인 발전을 거듭해왔으나 시약의 국산화 비율은 아직 많이 미흡한 상황이다. 최근 인화제약에서는 자동화학분석기용 액상 시약인 Liqchem®을 개발하였기에 이를 평가해보고자 하였다.<br> 방법: 임상화학검사 중 가장 많이 시행되는 AST, ALT, GGT, ALP, BUN, Cr, Glu, IP, TG, UA, T-Bil, D-Bil 및 HDL-C 등 13가지 검사 항목에 대하여 NCCLS guideline에 따라 정밀도, 직선성 및 기존에 사용하고 있던 외국 시약과의 상관성을 평가하였다.<br> 결과: 검사중, 검사간, 일간 및 전체 정밀도는 4% 미만으로 비교적 우수하였고, 직선성 검증에서는 모든 검사 항목에서 기울기 0.995-1.0534 이내, 결정계수(R²) 0.9948-1.0000으로 측정한 범위에서 매우 우수한 직선성을 보였다. 또한, 기존에 사용하고 있던 외국산 시약으로 측정한 결과와 비교한 상관성 검증에서도 모든 검사 항목에서 상관계수(R) 0.946-0.999로 우수한 상관성을 보였다.<br> 결론: 이상의 평가 결과 인화 Liqchem® 시약은 우수한 정밀도, 직선성 및 외국 시약과의 상관성을 보여 기존에 사용하고 있던 외국 시약을 대체하여 임상병리 검사실에서 사용하는데 무리가 없을 것으로 판단되었다. Background: When a new reagent for chemistry autoanalyzer is developed, it is essential to evaluate the reagent before utilization. As In Wha, Inc. has just developed new Liqchem® reagent for the chemistry autoanalyzer, we tried to evaluate the reagent.<br> Methods: Thirteen kinds of reagents for AST, ALT, GGT, ALP, BUN, Cr, Glu, IP, TG, UA, T-Bil, D-Bil, and HDL-C were evaluated for precision and linearity following the guidelines of NCCLS EP5-A and EP6-P, respectively. Comparison study with foreign reagents was also performed according to the NCCLS EP9-A guideline.<br> Results: Coefficient of variations (CV) of within-run, between-run, between-day, and total precision were less than 4% for all analytes. And the linearity data were acceptable (R²=0.9948~1.0000) for all test items. In addition, good correlation with foreign reagent (R=0.946~0.999) was observed for all analytes.<br> Conclusions: In Wha Liqchem® reagent for the chemistry autoanalyzer showed good precision, linearity, and correlation with foreign reagents that could be an alternative of currently used foreign reagent.

DiaSys 자동화학시약의 평가

김영기,김석수,마경란 대한임상검사정도관리학회 2001 臨床檢査와 精度管理 Vol.23 No.1

질량분석기를 활용한 막 단백질 비교분석: High-speed Centrifuge법과 Reagent-based법

이지영,석애은,박아름,문소라,강희규 대한임상검사과학회 2019 대한임상검사과학회지(KJCLS) Vol.51 No.1

Membrane proteins are involved in many common diseases, including heart disease and cancer. In various disease states, such as cancer, abnormal signaling pathways that are related to the membrane proteins cause the cells to divide out of control and the expression of membrane proteins can be altered. Membrane proteins have the hydrophobic environment of a lipid bilayer, which makes an analysis of the membrane proteins notoriously difficult. Therefore, this study evaluated the efficacy of two different methods for optimal membrane protein extraction. High-speed centrifuge and reagent-based method with a -/+ filter aided sample preparation (FASP) were compared. As a result, the high-speed centrifuge method is quite effective in analyzing the mitochondrial inner membranes, while the reagent-based method is useful for endoplasmic reticulum membrane analysis. In addition, the function of the membrane proteins extracted from the two methods were analyzed using GeneGo software. GO processes showed that the endoplasmic reticulum-related responses had higher significance in the reagent-based method. An analysis of the process networks showed that one cluster in the high-speed centrifuge method and four clusters in the reagent-based method were visualized. In conclusion, the two methods are useful for the analysis of different subcellular membrane proteins, and are expected to assist in selecting the membrane protein extraction method by considering the target subcellular membrane proteins for study. 막 단백질은 심장질환, 암과 같은 우리 주변에서 흔히 발생하는 질병에 관련되어 있다. 이러한 암과 같은 특정한 질환 상태에서, 막 단백질과 관련된 신호 전달의 비정상은 세포분열을 통제하지 못하고 증가시킬 수 있으며 막 단백질의 발현에 변화가 생긴다. 막 단백질은 지질 이중층으로 이루어진 소수성 환경을 가지고 있어 불안정하기 때문에 막 단백질을 추출해서 연구를 수행하는데 어려움이 있다. 이번 연구에서는 최적화된 막 단백질추출법을 확인하고자 서로 다른 두 가지 추출법의 효율성을 평가하였다. 두 가지 방법으로, high-speed centrifuge법과 reagent법이비교되었다. 비교 분석결과, 미토콘드리아 내막 단백질 분석에는 high-speed centrifuge법이 효율적이고, 소포체막 단백질 분석에는 reagent법이 유용함을 확인하였다. 게다가유전자 온톨로지 소프트웨어를 이용해서 추출된 막 단백질의기능분석을 진행하였을 때, 유전자 온톨로지는 reagent법에서소포체 막 단백질에 연관된 반응이 활성화 되는 것을 확인할 수있었다. 프로세스 네트워크 분석에서, high-speed centrifuge 법에서는 하나의 클러스터를 형성화는 반면, reagent법에서는네 개의 클러스터를 형성하는 것을 시각화하여 확인하였다. 결론적으로, 두 가지 분석법은 서로 다른 하위 막 단백질의 분석에유용함을 확인할 수 있었다. 이러한 결과를 토대로, 막 단백질을분석할 때, 표적의 세부 막 단백질을 고려하여 방법론을 선택하는데 도움을 줄 것으로 기대된다.

일반화학검사용 DiaSys 시약의 평가

황석연 ( Seock Yeon Hwang ),홍승복 ( Seung Bok Hong ),장철수 ( Cheoul Soo Chang ) 대한임상검사과학회 2001 대한임상검사과학회지(KJCLS) Vol.33 No.2

We evaluated the recently introduced DiaSys (DiaSys Diagnostic systems Intemational, Gennany) reagents for the routine chemical tests and compared with the reagents used in Chllllgbuk National University Hospital. We analyzed 15 items for the evaluation of precisions, linearities and recovery rates. The comparison study between the current reagents used in Chllllgbuk National University Hospital and DiaSys reagents were also performed for those items. The linearities were good in all items, and the recoverγ rates were acceptable results. The coefficients of variation (CV) of within-run and total precision were less than 5% in all items. The correlation analysis revealed good results in most items, except calcium showing the correlation coefficient of 0.9551. DiaSys reagents for the routine chemical tests showed good precisions, linearities, recovery rates and correlations with the currently used reagents. We conclude that these reagents are suitable to the substitute for the currently used reagents.