전신성홍반성낭창과 류마티스양관절염에서의 IL-6의 의의 : Systemic Lupus Erythematosus and Rheumatoid Arthritis

이정수,정화순 梨花女子大學校 醫科大學 醫科學硏究所 1999 EMJ (Ewha medical journal) Vol.22 No.2

IL-6는 단구, 대식구, T 및 B림프구와 기타 일부 세포에서 생성되는 26kDa의 싸이토카인으로서 면역작용, 감염 및 염증반응 등에서 다양한 기능을 가진다. IL-6는 간(肝)세포를 자극하여 피브리노겐과 같은 혈장단백의 생성을 유도하여 간접적으로 염증반응에 관여한다. IL-4와 IL-5에 의해 활성화된 B세포에 직접 작용하여 면역글로불린 class switching등의 마지막 분화과정을 촉진시키고 M, G, A형의 면역글로불린의 생성을 유도한다. 이는 IL-6가 면역계의 이상을 특징으로 하는 자가면역 질환의 발생과 임상양상의 발현에 관여하고 있을 가능성을 시사한다. 따라서 자가면역 질환에서 IL-6의 병인론적인 역할을 알아보고 혈청 IL-6의 활동성 지표로서의 가치를 평가하기 위하여 1995년 3월부터 1997년 9월까지 이화여자대학교 의과대학부속 목동병원에서 추적관찰중인 전신성홍반성낭창환자의 30검체와 류마티스양관절염환자의 26검체를 대상으로 혈청 IL-6와 적혈구침강속도, C-반응단백, 항 dsDNA, C3, C4 및 류마티스양인자를 측정하여 다음과 같은 결과를 얻었다. 1) 전신성홍반성낭창환자의 혈청 IL-6치는 평균(±표준편차)3.94土4.40pg/mL로 정상대조군의 1.33土l.72pg/mL 보다 통계적으로 유의하게 높았으며(p=0.0032) 류마티스양관절염환자 IL-6치도 8.22±5.17pg/mL로 정상대조군에 비하여 통계적으로 유의하게 높았다(p=0.0001). 2) 전신성홍반성낭창환자에서 질병의 활동성을 반영하는 것으로 알려진 항 dsDNA, C-반응단백, 적혈구침강속도, C3, C4치와 IL-6치를 비교하였을 때 항 dsDNA에서만 통계적으로 유의한 상관관계가 있었으며(p=0.038) 다른 항목과는 유의한 상관관계를 찾을 수 없었다. 6명의 전신성홍반성낭창 환자에서 질병의 경과에 따라 반복적으로 시행한 검사치에서도 질병의 활동성을 나타내는 검사치들과 IL-6치간에 명확한 상관관계를 찾을 수 없었다. 3) 류마티스양관절염 환자에서 C-반응단백, 류마티스양인자와 IL-6치를 비교하였으나 통계적으로 유의한 상관관계는 없었다. 본 연구를 통하여 전신성홍반성난창 및 류마티스양관절염 환자에서 혈청 IL-6치가 정상 대조군에 비하여 현저하게 증가되어 있음을 확인하였다. 그러나 질병의 경과에 따라 반복적으로 시행한 검사치에서 질병의 활동성을 나타내는 검사치들과 IL-6치간에 명확한 상관관계가 없는 것으로 보아 혈청 IL-6치가 질병의 활동성 지표로 사용되기는 어려울 것으로 생각된다. Systemic lupus erythematosus(SLE) and rheumatoid arthritis(RA) are the widely recognized rheumatic diseases of unknown etiology in which extensive immune dysfunction has been reported. Cytokines are considered to be the most important secretions of the immune system that participate in a variety of cellular, inflammatory and pathogenic processes in human disease. Since imbalance of the cytokine network in autoimmune disease may be detrimental for the severity or clinical manifestation of the disease, I determined serum level of IL-6 in patients with SLE, RA, and normal controls. The results were as follows: 1) The serum levels of IL-6 in patients with SLE(p=0.0032) and RA(p=O.0001) were significantly higher than those of normal controls. 2) The serum levels of ESR, CRP, and complements did not correlated with serum IL-6 levels. Only the levels of anti-dsDNA in patients with SLE showed correlation with that of serum IL-6. And the serial follow-up of serum IL-6 levels in 6 systemic lupus erythematosus patients show no significant correlation. 3) There were no significant correlation between serum IL-6 of RA patients and disease activity markers such as CRP and rheumatoid factors. This results indicate that serum IL-6 levels of SLE and RA patients were significantly higher than that of normal controls, and needs further study to be used as a marker for disease activity.

조혈세포 성장인자 IL-3, IL-6 및 SCF가 마우스 거핵구집락형성에 미치는 영향

이은희,김옥경 大韓血液學會 1995 大韓血液學會誌 Vol.30 No.2

Effect of IL-6 on Osteoclast Generation and Regulation of Its Production in Osteoblastic Cells

Shin, Dong-In,Baek, Jeong-Hwa,Ko, Seung-Hee,Kim, Gwan-Shik The Official Publication of Korean Academy of Oral 1996 International Journal of Oral Biology Vol.20 No.1

Interleukin-6 (IL-6) is a multifunctional cytokine that known to be synthesized by osteoblasts and suggested to be an important regulator of bone resorption in some physiologic and/or pathologic conditions. However, the mechanism of cation of IL-6 responsible for bone resorption in vitro and role as a possible mediator of bone resorptive action of systemic or local osteotropic agents are not clear yet. Therefore, to further understand the mechanism of IL-6 action on bone resorption, we examined the effects of IL-6 on the generation of osteoclast-like cells using mouse bone marrow culture system. We also observed the regulation of IL-6 secretion in human osteoblastic cells by several cytokines and systemic osteotropic hormones, known to stimulate or inhibit bone resorption. To observe the effects of IL-6 on the generation of osteoclast-like cells, mouse bone marrow cells were isolated from femurs and tibiae of 4 to 6 week-old mice, plated at 3.0×10^6 cells/well in 24-well plates and cultured for 8 days. In order to examine the role of IL-6 in osteoclast-like multinucleated cell (MNC) generation, bone marrow cells were treated with IL-6 alone or in combination with prostaglandin E_2(PGE_2). After culture, cells were stained for tartrate-resistant acid phosphatase (TRAP), a marker enzyme of osteoclasts, according to the modified method of Burstone. The TRAP-positive MNCs, which have 3 or more nuclei, were counted. Also we observed the regulation of IL-6 secretion in human osteoblastic cells (MG-63) by several osteotropic agents using the quantitative enzyme-linked immunosorbent assay. The present study showed that IL-6 alone did not generate TRAP-positive MNCs, but significantly enhanced the generation of TRAP-positive MNCs induced by PGE_2(10^-6 M) in a dose-dependent manner. Though the augmenting effect of IL-6 was significant through all the culture period, it was greater at later period of culture. And augmenting effect of IL-6 on generation of TRAP-positive MNCs significantly appeared from the 6th-day of culture. These results suggest that IL-6 enhances the effect of PGE_2 on TRAP-positive MNC generation by stimulating both differentiation and fusion of osteoclast precursors. On the otherhand, bone resorbing cytokines such as interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α) stimulated the secretion of IL-6 from MG-63 cells, but interferon-γ(IFN-γ), known to be an inhibitor of bone resorption, significantly inhibited the TNF-α-stimulated secretion of IL-6. However, parathyroid hormone, 1,25(OH)_2-vitamin D_3, and PGE_2, well known bone resorbing agents, did not induce the secretion of IL-6 from MG-63 cells. Taken together, these results suggest that IL-6 may play an important role in bone resorption by regulating the generation of osteoclasts and at least partly, mediate the bone modulating effects of several cytokines.

인간 자궁내막에서 IL-6 및 IL-6 수용체의 발현에 관한 연구

맹신숙(SS Maeng),조지연(JY Jo),김수연(SY Kim),정혜원(HW Chung),전선희(SH Chun),안정자(JJ Ahn),김성숙(HS Kim) 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.11

Endometrial cells undoergo a series of proliferative and secretory changes. These changes are precisely controlled and prepare endometrium for implantation of the blastocyst. It is thought that these changes are primarily dirven by two steroid hormones, estrogen and progesterone. However, recently it is suggested that many of the endometrial functions are controlled by various cytokines and reported presence of IL-1α, IL-1β, IRAP, IL-6, TGF-α proteins is human endometrium throughout the menstrual phase. Endometrial cells produced IL-6 in response to other inflammation-associated cytokines such as IL-1αorβ, TNF-α, IFN-γ. This secretion was strongly inhibited by 17-β estradiol. This established a novel li-nk between the endocrine and the immune systems. Therefore, this study was designed to examine the cell and site specific distribution of IL-6 and IL-6 receptor and the role of IL-6 in human endometrium during menstrual cycle. Endometrial tissues were obtained from hysterectomy specimens from normally cycling 44 patients in Ewha Womans University Hospital during past 14 months(1995. 1.1~1996. 2.29). The uteri were removed for abnormalities other than endometrial origin including uterine leiomyomas and cervical or ovarian lesions. Immunohistochemical staining was performed for IL-6 and IL-6 receptor in endometrial tissues. The results were as follows: 1. During the proliferative phase, expression of IL-6 was weak to absent in endometrial glands and stroma. 2. During the secretory phase, expression of IL-6 was stronger than that of proliferative phase and progressively increased in the mid- to late secretory phases. Expression of epithelial IL-6 was stronger than stroma. 3. IL-6 receptor was expressed primarily in endometrial glands and to a lesser extent in stroma. Expression of IL-6 receptor did not change throughout the menstrual cycle. Accordingly, this study show a cell and site specific distribution of IL-6 and IL-6 receptor in human endometrium through menstrual cycle. The menstrual cycle dependent expression of IL-6 suggest that this cytokine was controlled by steroid hormones. The menstrual cycle independent expression of IL-6 receptor suggest that IL-6 may act on endometrial function by autocrine and/or paracrine mechanisms. Increased expression of IL-6 in mid- to lat secretory phase suggest that IL-6 may be implicated in the induction of changes in human endometrium during the secretory/menstrual phase.

Mouse L Cell에서의 외래 유전자 유래 단백질의 생산

최윤희,岸本忠三,최차용 한국산업미생물학회 1993 한국미생물·생명공학회지 Vol.21 No.5

유전자 재조합 동물세포를 제작하여 외래 유전자 유래의 단백질을 생산하고자 그 model system으로써 부착성 동물 세포인 mouse L cell과 pBMG Neo vector, 그리고 외래 유전자로는 hNF-IL6를 선택하였다. hNF-IL6는 hIL-6 유전자의 promoter 서열에 결합하는 성질에 의해 분리된 핵단백질로서 IL-6의 전자조절 인자로 밝혀져 있다. 이 hNF-IL6는 다른 면역 관계 단백지의 전사 조절 영역에도 결합함이 밝혀져 그 중요성이 부각되고 있으나 재조합 대장균에서는 발현시 너무 빨리 분해되어 생산이 어려웠다. Plasmid내의 neomycin resistance 특성을 이용한 1차 screening, 그리고 southern blot analysis를 이용한 2차 screening에 걸쳐 hNF-IL6 발현 vector가 도입된 재조합 clone을 선택하였으며, 전사 및 발현를 확인하였고 그 발현이 MT promoter 특유의 유도기작에 따르는 것도 알 수 있었다. 생산된 hNF-IL6는 핵내에 존재함으로써 본래의 핵단백질 특성을 유지하고 있었으며 DNA binding activity도 확인되었다. 따라서 pBMG Neo vector를 사용, mouse L cell에서 발현된 외래 단백질 hNF-IL6가 본래의 활성도 유지함 대량 생산된 것을 알 수 있었고, 동물 세포에서의 외래 유전자 발현에 대한 이 system의 응용 가능성도 확인할 수 있었다. Some interleukin 6 (IL-6) transcription control factors were reported as the regulator of IL-6 expression. A nuclear protein bound to interleukin 1 (IL-1) responsive element in the IL-6 promoter region was named NF-IL-6 (nuclear factor for IL-6). This NF-IL6 was known to be very important as a transcription factor for various immuno-protein as well as for IL-6. The human NF-IL6 genes were transfected into the mouse L cells under the metallothionein promoter (MT promoter) to establish a model system for the expression of foreign gene in the mammalian cell line. Over a thousand of neomycin-resistant clones were obtained by using electroporation method, and some positive clones were screened with Southern blotting. After the selection of LBM6104 clone with high copy numbers, the expression of human NF-IL6 under the control of the MT promoter was confirmed by Northern and Western blot analysis. The MT promoter vector system became quite useful for a setup of foreign protein expression in the mammalian cell lines.

Evaluation of ‘TNF-α, IL-6, and MMP-9’ Test Kit for Screening of Meibomian Dysfunction in Patients with Inflammatory Dry Eye Syndrome

Min-Hye Park,Jung-Eun Park,Jang-Won Byun,Min-Ji Choi,Il-Hoon Cho,Myeong-Jin Jeong,Yoon-Jung Choy,Koon-Ja Lee 대한시과학회 2020 대한시과학회지 Vol.22 No.1

목적 : 마이봄샘기능저하증(meibomian gland dysfunction, MGD)을 수반하는 염증성 건성안의 감별진단에 대한 ‘TNF-α, IL-6, MMP-9’ 검사키트의 유용성을 평가하였다. 방법 : 건성안 이외의 안질환이 없는 20~30대 중 OSDI 설문 검사에 따른 건성안 총 118안을 대상하였고, 결막낭 메니스커스로부터 소량의 눈물을 채취하여 TNF-α, IL-6 및 MMP-9 검사를 하였다. 각막염색과 결막충혈 이 모두 Grade 1 이상인 경우는 염증성 건성안으로, 마이봄샘폐쇄와 마이봄샘구멍막힘이 모두 grade 1 이상인 경우는 MGD 관련 건성안으로 평가하였다. 염증성 건성안 및 MGD와 TNF-α, IL-6, MMP-9과의 상관성은 카 이제곱검정(Chi-square test)으로 분석하였고, ‘TNF-α, IL-6, MMP-9’ 검사키트의 염증성 건성안과 MGD를 수반하는 염증성 건성안 감별능력은 ROC 커브를 이용하여 민감도, 특이도 및 AUC(Area under the curve)를 구하고 정확도를 평가하였다. 결과 : 염증성 건성안은 TNF-α와 IL-6와 유의한 상관성을 보였고(p<.050), ‘TNF-α, IL-6, MMP-9’ 검사 키트는 MMP-9 검사키트와 80.20%의 높은 일치도를 나타냈으나(p<.050), 염증성 건성안 감별에 대한 민감도, 특이도, 정확도는 MMP-9 검사키트보다 낮았다. MGD는 MMP-9 검사와 상관성을 보이지 않았고, TNF-α와 IL-6 검사와는 유의한 상관성을 보였으며, MGD 감별에 대한 민감도, 특이도, 정확도는 각각 85.50%, 34.70%, 0.601, 85.50%, 32.70%, 0.591로 나타났다. MGD 수반한 염증성 건성안 감별에 대한 ‘TNF-α, IL-6, MMP-9’ 검사키트의 민감도, 특이도 및 정확도는 100.00%, 34.10%, 0.670로 MMP-9 검사키트보다 더 높았다. 결론 : MGD 진단에는 TNF-α, IL-6 검사가 유용하며, ‘TNF-α, IL-6, MMP-9’ 검사키트는 MGD를 수반한 염증성 건성안 평가에 유용할 것으로 사료된다. Purpose : To evaluated the ‘TNF-α, IL-6, MMP-9’ test kit for screening of inflammatory dry eye and IDE (inflammatory dry eye) with MGD (meibomian gland dysfunction). Methods : A total of 118 dry eyes were selected using OSDI (ocular surface disease index) questionnaire among participated 20~30s without ophthalmologic diseases except for dry eye. Small amount of tear obtained from meniscus of the conjunctiva were tested with TNF-α, IL-6, and MMP-9 kit. IDE refers to the criteria which specifies the corneal staining and conjunctival hyperemia more than grade 1 and MGD refers to the criteria which specifies meibomian gland blockage and meibomian orifice obstruction with more than grade 1. Chi-square test was performed to analyze the correlation between the IDE, MGD and the results of ‘TNF-α, IL-6, MMP-9’ tests. and ROC (receiver operate characteristics) curve was used for the sensitivity, specificity and AUC (area under the curve) for the accuracy of ‘TNF-α, IL-6, MMP-9’ tests. Results : TNF-α and IL-6 were significantly correlated with IDE (p<.050) and ‘TNF-α, IL-6, MMP-9’ test kit showed a high agreement of 80.20% with MMP-9 test kit(p<.050) although the accuracy was lower than MMP-9 test kit. The MMP-9 showed no correlation with MGD, however TNF-α, IL-6 were significantly correlated with MGD (p<.050). sensitivity, specificity, and AUC of TNF-α, IL-6 tests for MGD were 85.50%, 34.70%, 0.601, 85.50%, 32.70%, and 0.591. The sensitivity, specificity, and AUC of ‘TNF-α, IL-6, MMP-9’ test kit for IDE with MGD were 100.00%, 34.10%, and 0.670, respectively, which shows higher accuracy than MMP-9. Conclusion : TNF-α and IL-6 tests are useful for the diagnosis of MGD, and ‘TNF-α, IL-6, MMP-9’ test kit is useful for screening IDE with MGD.

임신말기 C₃H쥐의 복강내 Lipopolysaccharide 주사가 태반, 태아 및 자궁내막에서의 IL-6, IL-13 및 TIMP-3의 발현에 미치는 영향

홍성란,문인걸,서주영,김의정,김성숙,한운섭 대한병리학회 1998 Journal of Pathology and Translational Medicine Vol.32 No.5

자연폐경여성에서 interleukin(IL)-6 유전자내 (CA) 반복다형성, 혈청 IL-6농도 및 골밀도와의 연관성

김정구(Jung Gu Kim),김석현(Seok Hyun Kim),최영민(Young Min Choi),문신용(Shin Yong Moon),이진용(Jing Yong Lee) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.3

Objective: To investigate the relationship between cytosine-adenosine(CA) polymorphism in interleukin-6 (IL-6) gene, serum IL-6 levels and bone mineral density (BMD). Methods : IL-6 CA polymorphism was analyzed by polyacrylamide-urea gel electrophoretic patterns in 300 postmenopausal Korean women. and direct DNA sequencing. Serum IL-6 was measured by enzyme linked immunosorbent assay, and BMD at the lumbar spine and proximal femur by dual energy X-ray absorptiometry. Results: Nine alleles were observed with product sizes ranging between 120-136 bp and 125 allele was the most common. There were no significant differences in serum IL-6 levels and BMD at the lumbar spine and proximal femur between the women who possessed at least one allele of each genotype and those who did not posses that allele. Serum IL-6 levels did not correlated with age, menopause duration, body mass index and BMD at the lumbar spine and proximal femur. IL-6 CA polymorphic pattern and serum IL-6 levels in osteoporotic women were not different from those in normal BMD or osteopenic women. Conclusions: IL-6 CA polymorphism has no relationship with serum IL-6 levels and BMD in postmenopausal Korean women.

Effect of TNF-α and IL-6 on Compact Bone-Derived Cells

Zhang Yiming,Li Xianqi,Chihara Takahiro,Dong Hongwei,Kagami Hideaki 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.3

Background: Although bone tissue engineering has already been applied clinically, its regeneration efficacy is not always sufficient. Local inflammatory cytokines are considered as the major factors that induce apoptosis of transplanted cells, thus leading to insufficient new bone formation. In this study, we focused on the effects of interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) on differentiation and apoptosis of compact bone-derived cells (CBDCs). Methods: CBDCs were obtained from mouse legs and cultured. The effects of TNF-α and/or IL-6 on the osteogenic differentiation and apoptosis of CBDCs were analyzed in vitro. To confirm the expression of local inflammatory cytokines in vivo, CBDCs were transplanted to the back of immunocompetent mice. Results: IL-6 exerted inconsistent effects on the expression of the different osteogenic markers tested, while significantly upregulating Fas. By contrast, the addition of TNF-α dramatically reduced the expression of all tested osteogenic markers and increased Fas expression. The highest dose of IL-6 could partially reverse the repressive effect of TNF-α, while the addition of IL-6 further increased Fas expression in CBDCs compared to TNF-α alone. The results from in vivo experiments showed the presence of transplants with and without new bone formation. The transplants without bone formation were characterized by higher IL-6 and lower IL-10 expression than those with bone formation, while the expression of TNF-α did not show notable difference. Conclusion: The results of this study suggest an important role for IL-6 in modulating the efficacy of bone tissue engineering, which can affect osteogenic cells both positively and negatively. Background: Although bone tissue engineering has already been applied clinically, its regeneration efficacy is not always sufficient. Local inflammatory cytokines are considered as the major factors that induce apoptosis of transplanted cells, thus leading to insufficient new bone formation. In this study, we focused on the effects of interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) on differentiation and apoptosis of compact bone-derived cells (CBDCs). Methods: CBDCs were obtained from mouse legs and cultured. The effects of TNF-α and/or IL-6 on the osteogenic differentiation and apoptosis of CBDCs were analyzed in vitro. To confirm the expression of local inflammatory cytokines in vivo, CBDCs were transplanted to the back of immunocompetent mice. Results: IL-6 exerted inconsistent effects on the expression of the different osteogenic markers tested, while significantly upregulating Fas. By contrast, the addition of TNF-α dramatically reduced the expression of all tested osteogenic markers and increased Fas expression. The highest dose of IL-6 could partially reverse the repressive effect of TNF-α, while the addition of IL-6 further increased Fas expression in CBDCs compared to TNF-α alone. The results from in vivo experiments showed the presence of transplants with and without new bone formation. The transplants without bone formation were characterized by higher IL-6 and lower IL-10 expression than those with bone formation, while the expression of TNF-α did not show notable difference. Conclusion: The results of this study suggest an important role for IL-6 in modulating the efficacy of bone tissue engineering, which can affect osteogenic cells both positively and negatively.

IgA 신증에서의 요중 IL-6와 TGF-β 분비와AII 수용체 차단제의 효과

이승준,한주영,김희진,이태원,임천규 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.3

Background:Urinary levels of cytokines may function as indices of renal disease activity. Methods:This study was designed to investigate the urinary excretion of IL-6 and TGF-β in the patients with IgA nephropathy(IgAN) and the effects of AII receptor blockers(ARB:losartan) on it. Results:The patients with IgAN(n=10) showed higher urinary excretion of IL-6 and TGF-β than normal controls(n=7). There is a positive correlation between IL-6 and TGF-β. Urinary excretion of IL- 6 was positively correlated with proteinuria in IgAN (IL-6:γ2=0.560; p<0.05) but not with serum creatinine. After 2 months of ARB therapy in IgAN patients, urinary excretion of IL-6 was decreased(IL-6:11.3±7.7 vs 5.3±3.3 pg/mg Cr, p<0.05). Positive correlations were noticed in ARB group between urinary cytokines and proteinuria. Conclusion:Our data suggest that the patients with IgAN showed higher urinary excretion of IL-6 and TGF-β, and the IL-6 excretion can be effectively reduced by ARB therapy. 배 경:증식세포인자인 IL-6와 경화증 성장인자인 TGF-β의 요 분비 측정이 질환의 활성도 및 예후 판단에 유용함이 제시되고 있다. 따라서 IgA 신증 환자들에서 IL-6와 TGF-β의 요중 분비를 측정하고 AII 수용체 차단제(ARB)의 치료 효과를 조사하였다. 방 법:IgA 신증 환자들(n=10)을 대상으로 ARB 치료 전과 2개월간의 치료 후에 각각 오전 10시경의 소변을 채취하여 저장한 후 enzyme-linked immunosorbent assay법을 이용하여 측정하였고 요중 크레아티닌 농도로 보정하였다. 결 과:ARB 치료 전 IgA 신증 환자들의 요중 IL-6와 TGF-β의 분비량은 다른 사구체질환군과 같이 대조군에 비해 유의하게 높았다. IgA 신증 환자들의 요중 IL-6와 TGF-β분비량 사이에는 양의 상관이 관찰되었다(p<0.01). 또한 요중 IL-6 분비와 단백뇨량은 통계학적으로 유의한 양의 상관을 나타내었다(p<0.05). 요중 IL-6 및 TGF-β 분비와 혈청 크레아티닌 수치와는 상관성이 없었다. IgA신증 환자들에서 2개월의 ARB 치료 후 요중 IL-6 분비량은 유의하게 감소하였다(p<0.05), TGF-β는 감소 경향을 보였으나 통계학적인 유의성이 없었다. ARB 치료군에서도 이들 세포인자들의 변화는 단백뇨량과 양의 상관을 보였다. 결 론:IgA 신증 환자들에서 요중 IL-6와 TGF-β의 분비가 증가하였고 이는 단백뇨량과 상관성이 있었다. ARB 치료는 병태생리적으로 IL-6 세포인자의 분비를 억제하는 한 효과적인 치료법이라고 생각된다.