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RISS 인기검색어
- 검색키워드
- 주제어 : Fragaria spp. (검색결과 3 건)
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Tao Wang,Dongya Huang,Baoyu Chen,Nini Mao,Yu-Shan Qiao,Muxiang Ji 한국유전학회 2018 Genes & Genomics Vol.40 No.3
Polyploidization always induces a series of changes in genome, transcriptome and epigenetics, of which changes in gene expression are the immediate causes of genotype alterations of polyploid plants. In our previous study on strawberry polyploidization, genes related to photosynthesis were found to undergo changes in gene expression and DNA methylation. Therefore, we chose 11 genes that were closely related to plant photosynthesis and analysed their expression during strawberry hybridization and chromosome doubling. Most genes of pentaploids showed expression levels between parents and were more similar to F. × ananassa. Gene expression levels of decaploids were higher than those of pentaploids and F. × ananassa. Different types of photosynthesis-related genes responded differently to hybridization and chromosome doubling. Chloroplast genes and regulatory genes showed complex responses. Structural genes of the photosynthetic system were expressed at a constant level and displayed a clear dosage effect. The methylation levels of one CG site on SIGE, which regulates expression of chloroplast genes, were negatively correlated with gene expression. In pentaploids and decaploids, more transcripts were from F. × ananassa than from F. viridis. The ratio of transcripts from from F. × ananassa to those from F. viridis was close to the ratio (4:1) of the genome of F. × ananassa to that of F. viridis in pentaploids and decaploids, but there were also some exceptions with obvious deviation.
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딸기 유전자원의 원예적 특성과 주성분 분석에 의한 품종군 분류
김대영(Dae-Young Kim),윤무경(Moo Kyung Yoon),곽정호(Jung-Ho Kwak),김태일(Tae Il Kim),김진한(Jin-Han Kim) 한국원예학회 2009 원예과학기술지 Vol.27 No.4
In order to promote the efficiency of strawberry breeding programs in Korea, we evaluated various horticultural traits of strawberry (Fragaria spp.) germplasms collected in Rural Development Administration from domestic to abroad, especially Japan and United States. Genetic relationships among accessions were carried out on 43 strawberry germplasms based on 26 horticultural traits by principal component (PC). The first three PCs represented 43.0% of the total variation in the data set. The first PC and second PC accounted for 20.2% and 13.0% of the total variance and separated the taxa essentially by characteristics related to plant height, leaf length, fruit width and weight. As the dendrogram of UPGMA (unweighted pair-group method using arithmetic averages) clustering based on 1 to 3 PCs, 43 strawberry cultivars were classified into five large groups (Ⅰ-Ⅴ) at the average distance 1.0 and three small groups of the large group Ⅰ at the average distance 0.7 in accordance with mainly plant vigor, fruit size and their parentage. The groups Ⅲ to V were mainly used as breeding materials in Korea; therefore, we need to make crosses these materials with cultivars in other groups like groups Ⅰ and Ⅱ to increase genetic variation and improve fruit quality in the future.
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Molecular Biological Analysis for the Identification of Meloidogyne Species on Strawberry in Korea
Heonil Kang,Namsook Park,Donggeun Kim,Insoo Choi 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04
This study is the identification of root-knot nematode (RKN), Meloidogyne hapla, from strawberry in Korea using molecular analyses. Strawberry plants showed localized stunting and galled roots. Molecular analyses of COⅡ/lrRNA, 28S rDNA D2-D3 segment and internal transcribed spacer (ITS) region were employed for the identification of Meloidogyne spp. Polymerase Chain Reaction (PCR) amplification of COⅡ/lrRNA region produced a single fragment ca 528bp. Restriction digestion of the amplified PCR products with Dra1 enzyme produced two fragments at 200 and 250bp indication M. hapla. 28S rDNA D2-D3 segment and ITS were cloned and sequenced. 28S rDNA D2-D3 segment and ITS region produced a single fragment of 1004bp and 560bp, respectively. In BLAST search in Genbank, all sequences accord with other known M. hapla. As a result, all of the RKN samples were M. hapla. This study suggests that the dominant species of RKN on strawberry is M. hapla in Korea.
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