Urokinase 투여가 Euglobulin Fibrinolytic Activity에 미치는 영향

김영선,오도연,홍세용 대한내과학회 1990 대한내과학회지 Vol.38 No.4

Euglobulin fibrinolytic activity was measured during urokinase infusion.(loading dose 200,000IU, maintenance dose 200,000IU/hr for five hours) by a wellcontrolled fibrin plate in seven cases of thrombosis(six cases of cerebral thrombosis and one case of deep vein thrombosis) with regard to the change of the blood fibrinogen PTT, TT and FDP. The basal fibrinolytic activity activity was lower than the normal control in five of the case. The fibrinolytic activity in euglobulin fraction increased during urokinase infusion in all cases, but the range of increase was variable(range: 27.4~8. 1 in case 2, 175.6~69.7 in Case 1). Fibrinolytic activity decreased below the basal level by two hours after the cessation of urokinase infusion in all cases. The basal level of fibrinogen concentration ranged from 335-880 mg/㎗ and decreased after the loading dose of urokinase(200,000IU) in all except case 2. The TT and/or PTT, measured in cases 1,4,6 and 7 related well to the fibrinogen level but was not prolonged more than three times the basal level through the urokinase infusion in all cases. Our results emphasize that an adequate dose of urokinase should be assessed by the fibrinolytic activity itself. If the fibrinolytic activity is evaluated only by a fibrinogen-related coagulation profile such as TT or PTT, the adequate fibrinolytic activity might by measured by the individual variance of the reserve of the fibrinogen synthesis.

Lumbricus Rubellus 가 인체의 섬유소 용해력에 미치는 영향

홍세용(S . Y . Hong),양동호(D . H . Yang),오도연(D . Y . Oh),박선양(S . Y . Park) 대한내과학회 1991 대한내과학회지 Vol.40 No.1

To evaluate the lumbrikinase effect on plasmin (ogen) related physiologic fibrinolytic activity, we measured euglobulin fibrinolytic activity, t-PA activity and t-PA antigen during lumbrikinase therapy and the results were compared with the data of control day of before and after lumbrikinase ingestion. Serial dilution of lumbrikinase showed the fibrinolytic activity on fibrin plate by dose dependent pattern between 375㎍/ml and 1㎍/ml concentration. In regard to the fibrinolytic area, 300㎍ of lunbrikinase equivalent to 25 unit of t-PA and 75㎍ of lumbrikinase equivalent to 5 unit of t-PA. Euglobulin fibrinolytic activity increased in all cases by two or three days after lumbrikinase ingestion but decreased to basal level 36 hours after discontinuation of lunbrikinase. The lumbrikinase added to plasma did not influence to the euglobulin fibrinolytic activity. t-PA ag was increased in 5 out of 6 cases and t-PA activity was increased all of the cases after lumbrikinase ingestion. There was direct correlation between euglobulin fibrinolytic activity and t-PA activity. This finding suggest that the increased fibrinolytic activity of euglobulin after lumbrikinase ingestion is due to increased t-PA rather than direct effect of lumbrikinase itself.

Low Molecular Weight Heparin 이 Fibrinolytic Activity 에 미치는 영향

홍세용(Sae Yong Hong),양동호(Dong Ho Yang) 대한내과학회 1991 대한내과학회지 Vol.40 No.4

To evaluate the influence of standard and low molecular weight heparin (LMWH) on intrinsic and extrinsic fibrinolytic activity, we measured total fibrinolytic activity in dextran sulfate euglobulin fraction, t-PA activity, and t-PA ag in plasma from 10 patients with cerebral infarction before and after heparin injection. The results were compared with that of a control day (on heparin injection day). The increase of total fibrinolytic activity in the 1.MWH group was significantly higher than in the standard heparin group (0. 025 < p < 0.05) and control group ((0.025<p<0,05). The t-PA activity increased in the at 1:00 P.M. samples in 3 cases of the standard heparin group 4 cases of the LMWH group and 4 cases of the saline group. But it decreased in 6 cases of the standard heparin group 6 cases of the LMWH group and 6 cases of the saline group. The change of t-PA activity and the rate of increase and decrease did not differ significantly between the groups (p>0.1 ANOVA). The t-PA ag of the 1:00 P.M. samples increased in 9 cases of the standard heparin group, 4 cases of the LMWH group and 7 cases of the saline group. But it decreased in 1 case of the standard heparin group, 6 cases of the LMWH group and 3 cases of the saline group, The change of t-PA ag and the rate of increase and decrease did not differ significantly between the group (p>0.1 ANOVA). The finding that LMWH in- creased the total fibrinolytic activity without an in- crease of extrinsic fibrinolytic activity suggests that LMWH increases intrinsic fibrinolytic activity.

유전자 cloning에 의한 Bacillus subtilis의 fibrinolytic enzyme 활성 변화

이홍석,유천권,이철수,강상모 한국산업미생물학회 2000 한국미생물·생명공학회지 Vol.28 No.1

구조유전자와 enhancer 유전자를 cloning 하여 fibrinolytic enzyme을 생산하는 B. subtilis K-54와 J-10의 fibrinolytic enzyme의 생산 능력을 높이기 위하여 이 유전자를 포함하는 재조합 vector를 만들어 형질전환하여 그 생산능의 변화를 조사하였다. aprN과 enhancer 유전자인 prtR 유전자의 primer를 제작하여 PCR한 결과, B. subtilis J-10주에서는 aprN과 prtR 유전자 band를 확인하였고 염기서열 분석결과 자체 promoter를 갖는 완전한 유전자임을 확인하여 cloning에 사용하였다. Cloning된 두 유전자를 가지고 B. subtilis J-10과 B. subtilis K-54를 형질전환하기 위해 E. coli/B. subtilis shuttle vector에 cloning하여 재조합 vector를 제작하였다. 그 결과 aprN 유전자를 갖는 pAPR2, prtR 유전자를 갖는 pENC2, 두 유전자 모두 가지는 pFLA1을 만들었으며 B. subtilis J-10과 K-54를 형질전환하여 재조합된 Bacillus속의 균주를 만들어 fibrinolytic activity의 생산량을 조사하였다. pAPR2과 pFLA1로 형질전환하였을 경우 각각 약 27.3 와 16%의 활성 증가를 보였으나 pENC2의 경우 활성 증가를 보이지 않았다. pENC2로 분리주인 B. subtilis K-54를 형질전환하였을 경우 약 5배 정도의 활성 증가를 보여 효소의 생산이 증가하였음을 확인할 수 있었다. The transformation of Bacillus subtilis K-54 and J-10 was carried out with constructed vectors containing structure and enhancer genes of aprN and prtR, to increase their fibrinolytic enzyme activity, Bands for the aprN and prtR genes were indentified from B. subtilis J-10 by PCR that was carried out with the constructed primers for the genes. In addition, the gene fragments contained promoter site based on the results of analysing their nucleotide sequence. The two gene fragments, aprN and prtR, obtained by the PCR, were, then, inserted to vector such as T-vector and E.coli/Bacillus shuttle vector. The constructed vector were designated as pAPR2 (aprN) pENC2(prtR) and pFLA1 (aprN and prtR), respectively. the constructed vector was used for transformation of the strains of B. subtilis J-10 and B. subtilis K-54 and the fibrinolytic activity of the transformed strains was investigated. The introduction of the vector, pAPR2 and pFLA1, resulted in the increase of fibrinolytic enzyme activity in B. subtilis J-10 by 27.3 % and 16 %, respectively. However, the introduction of pENC2 to B. subtilis J-10 did not seem to induce increase of the enzyme activity. The strain of B. subtilis K-54 transformed with pENC2 showed an increased fibrinolytic activity by 5 folds compared with that of the original strain of B. subtilis K-54.

Change of Fibrinolytic and Antioxidative Activities of Codonopsis lanceolata According to Various Storage Conditions, and Heat or Salt Treatments

김준호,--,-- 대한의생명과학회 2005 Biomedical Science Letters Vol.11 No.1

We investigated the changes of fibrinolytic and antioxidative activities of wild or cultivated Codonopsis lanceolata, which were packed in woven polypropylene (WP) film or low density polyethylene (LDPE) film and stored for 0, 15, and 30 days at refrigerated (2~4℃) or room (18~20℃) temperature (So we have 16 samples, and wrote them as the abbreviated words such as W-WP-RE-15, W-WP-RO-15, W-LDPE-RE-15, W-LDPE-RO-15, W-WP-RE-30, W-WP-RO-30, W-LDPE-RE-30, W-LDPE-RO-30, C-WP-RE-15, C-WP-RO-15, C-LDPE-RE-15, C-LDPE-RO-15, C-WP-RE-30, C-WP-RO-30, C-LDPE-RE-30, C-LDPE-RO-30). Fibrinolytic activity of fresh Codonopsis lanceolata cultivated in the mountain or field were 0.8 unit (plasmin unit/ml) or 7.3 units, respectively. In descending order, the activities of wild Codonopsis lanceolata stored for 15 days were as followed ; LDPE-RE (0.70 unit), WP-RO (0.52 unit), WP-RE (0.45 unit), and LDPE-RO (0.30 unit). After 30 days, fibrinolytic activities of them decreased to 0.47 unit (LDPE-RE), 0.28 unit (WP-RO), 0.21 unit (WP-RE), and 0.30 unit (LDPE-RO). Considering from the point of fibrinolytic activity, the optimal storage condition of wild Codonopsis lanceolata was packing with LDPE film and storing at 4℃. The change of fibrinolytic activities of Codonopsis lanceolata cultivated in the field revealed the similar trend as wild samples, but the fibrinolytic activities of 30 days-stored samples were maintained better than the wild Codonopsis lanceolata. Fibinolytic activity of wild Codonopsis lanceolata was increased by heating for 5 min at 100℃ and decreased by addition of NaCl. Antioxidative activities of Codonopsis lanceolata were also compared from the electron donating activity. Fresh Codonopsis lanceolata had about 70% of electron donating activity. Independent of cultivation area, electron donating activity dropped to 19~74% (wild Codonopsis lanceolata) and 27~59% (cultivated Codonopsis lanceolata) during 15 days storage. But after 30 days storage, we obtained the unexpected results, which meant that the activities were higher activities than 15 days-stored samples or even though the fresh samples. In general, Codonopsis lanceolata could maintain antioxidative activities most strongly with LDPE film and chilled condition.

아위버섯 추출물의 혈전용해, 트롬빈저해, 항산화 및 항염증 활성

김은정,김준호 한국버섯학회 2015 한국버섯학회지 Vol.13 No.1

아위버섯을 혈관계질환 예방과 치료를 위한 기능성 식품 개발에 이용하기 위한 기초 자료를 얻기 위해 아위버섯 물추출물과 유기용매 분획물의 혈전용해활성과 트롬빈 저해활성, 항산화활성 및 항염증활성을 확인하였다. 에틸아세테이트 분획물은 1.33 plasmin unit의 높은 혈전용해 활성과, 94.45%의 높은 트롬빈 저해활성을 나타냈으며, 물 추출물은 37.01%의 항산화활성을 나타냈으며, 클로로포름 분획물은 98.13%의 가장 높은 항염증활성을 나타냈다. 실험 결과로부터 아위버섯의 에틸아세테이트 분획물은 높은 혈전용해활성, 트롬빈 저해활성 그리고 클로로포름 분획물은 높은 항염증활성을 나타내 심혈관계 질환 관련 치료나 예방을 위한 기능성식품 개발에 이용 가능할 것으로 기대된다. Our study investigated the fibrinolytic, thrombin inhibitory, anti-oxidative and anti-inflammatory activities of the water extract and solvent fractions isolated from Pleurotus ferulea. Fibrinolytic activity was investigated using the fibrin plate method. Thrombin inhibitory activity was used to analyze thrombin inhibitor assay. The DPPH assay was used to estimate anti-oxidative activity. Inhibition of NO production was measured for anti-inflammatory activity in LPS-activated murine RAW 264.7 macrophage cells. An MTS assay was used to evaluate the effects of the water extract and solvent fractions isolated from Pleurotus ferulea on cell viability. Our results showed the fibrinolytic activity to be strong in the ethyl acetate fraction at 1.33 plasmin units. The ethyl acetate fraction also showed high thrombin inhibitory activity at 94.45%. The anti-oxidative activity of the water extract was 37.01% and the anti-inflammatory activity of the chloroform fraction was 98.13%. These findings suggest that Pleurotus ferulea's extract and fractions could be applicable in the development of functional foods for the treatment and prevention of cardiovascular diseases.

아위버섯 추출물의 혈전용해, 트롬빈저해, 항산화 및 항염증 활성

김은정,김준호 한국버섯학회 2015 한국버섯학회지 Vol.13 No.1

Our study investigated the fibrinolytic, thrombin inhibitory, anti-oxidative and anti-inflammatory activities of the water extract and solvent fractions isolated from Pleurotus ferulea. Fibrinolytic activity was investigated using the fibrin plate method. Thrombin inhibitory activity was used to analyze thrombin inhibitor assay. The DPPH assay was used to estimate antioxidative activity. Inhibition of NO production was measured for anti-inflammatory activity in LPS-activated murine RAW 264.7 macrophage cells. An MTS assay was used to evaluate the effects of the water extract and solvent fractions isolated from Pleurotus ferulea on cell viability. Our results showed the fibrinolytic activity to be strong in the ethyl acetate fraction at 1.33 plasmin units. The ethyl acetate fraction also showed high thrombin inhibitory activity at 94.45%. The anti-oxidative activity of the water extract was 37.01% and the anti-inflammatory activity of the chloroform fraction was 98.13%. These findings suggest that Pleurotus ferulea's extract and fractions could be applicable in the development of functional foods for the treatment and prevention of cardiovascular diseases.

더덕과 일부 한약재 열수추출물의 혼합 비율에 따른 생리활성

오혜숙,김준호 한국지역사회생활과학회 2007 한국지역사회생활과학회지 Vol.18 No.3

The aim of this study was to investigate the physiological functionalities of Codonopsis lanceolata, Glycyrrhiza uralensis, Chaenomeles sinensis, Crataegus pinnafida, and their mixtures. We also determined their antioxidative, fibrinolytic, and αglucosidase inhibitory activities. The antioxidative activities of Codonopsis lanceolata, Glycyrrhiza uralensis, Chaenomeles sinensis, and Crataegus pinnafida were 79%, 88.3%, 89.9%, and 89.3% respectively. Their fibrinolytic activities were 0.80plasmin unit/ml, 0.57 plasmin unit/ml, 0.52 plasmin unit/ml, and 0.53 plasmin unit/ml respectively. The αglucosidase inhibitory activity of Codonopsis lanceolata was 25%. The 10fold diluents of Glycyrrhiza uralensis, Chaenomeles sinensis, and Crataegus pinnafida showed αglucosidase inhibitory activities of 93.6%, 65.3%, and 61.3% respectively. In antioxidative activity tests of the medicinal plants mixtures at various ratios, the mixtures of Glycyrrhiza uralensis, Chaenomeles sinensis, and Crataegus pinnafida with Codonopsis lanceolata showed antioxidative activities of approximately 90%. In fibrinolytic activity tests mixtures(1:1) of Codonopsis lanceolata with Chaenomeles sinensis and Crataegus pinnafida exhibited increases of 23% and 24% in activity respectively. In αglucosidase inhibitory activity tests, a mixture (4:1) of Codonopsis lanceolata and 10fold diluted Glycyrrhiza uralensis showed an inhibitory activity of 98%, a mixture (3:1) of Codonopsis lanceolata and 10fold diluted Chaenomeles sinensis showed an inhibitory activity of 69.6%, and a mixture (1:1) of Codonopsis lanceolata and 10fold diluted Crataegus pinnafida showed an inhibitory activity of 50.2%. In conclusion, the mixtures of Glycyrrhiza uralensis, Chaenomeles sinensis, and Crataegus pinnafida with Codonopsis lanceolata will be used as a material for the development of biofunctional foods.

김치의 혈전용해작용

강정옥,정영기,김정옥,양웅석,공인수 동의대학교 기초과학연구소 1996 基礎科學硏究論文集 Vol.6 No.1

Fibrinolytic activity of fermented kimchi and ingredients used for the preparation were assayed by measuring the lysis area on plasminogen-rich fibrin plate. Fermented kimchi and pickled fish sauces from changlan, prwan, and anchovy showed the activity, and the activity of pickled fish sauces were high in the order of pickled changlan, pickled anchovy(pickled mulchi), and pickled prawn. However, the activity of kimchi may not be attributed to pickled fish sauce because kimchi containing fish sauce did not possess activities before fermentation. To investigate fibrinolytic agents in the kimchi if protein, the activities were determined from the samples heated for 30min at 100℃. There was no changes in the activities before and after heat treatment indicating the agents may be components other than protein. Since major changes occur during kimchi fermentation were increased sour taste due to production of organic acids such as lactic, citric, oxalic, and succinic acids, the authentic organic acids present were examined for fibrinolytic activities. The results indicates that the major component posses the activity is lactic acid.

Development of Functional Beverage (SanYa) from Fermented Medical Plants and Evaluation of Its Physiological Activities

Eun Kyung Cho(조은경),Hyo Ju Song(송효주),Hea Eun Cho(조혜은),In Soon Choi(최인순),Young Ju Choi(최영주) 한국생명과학회 2010 생명과학회지 Vol.20 No.1

지금까지의 연구에 의하면 산야 발효액에 대한 보고가 미비하므로 본 연구에서는 산야의 기능성에 관한 연구를 위하여 산야 발효액으로 여러 가지 생리활성과 면역활성에 대하여 분석하였다. 우선, 산야의 항산화 활성을 측정하기 위하여 DPPH 활성과 플라보노이드 함량을 측정하였다. 그 결과 DPPH법을 통해 측정한 산야 발효액의 항산화력은 94.3%의 radical 소거능을 나타내었다. BHA와 비교 했을 때 유사한 항산화력이 측정되었는데, 이것은 항산화 활성이 높은 것으로 산야의 항산화력에 관한 높은 이용가치를 의미한다. 산야 발효액의 총 플라보이드 함량은 4.646 ㎎/g으로 높았으며, 이는 높은 항산화능과 연관성이 있는 것으로 나타났다. 산야 발효액과 면역활성과의 연관성은 NO 합성률과 LPS에 의해 유도되는 NO 합성 저해률 분석으로 조사되었다. 그 결과, 산야 발효액은 무려 11배의 높은 NO 합성율을 보였다. 또한 LPS에 의해 유도된 NO 합성 저해율은 56%로 나타나 산야의 높은 면역효과를 증명하고 있다. 혈전분해능에 대한 산야 발효액의 분석 결과는 혈전용해제로 알려져 있는 plasmin보다 높은 활성을 보이고 있다. SOD 유사활성은 42.2%로 비교적 높은 SOD 유사활성을 나타내었고, xanthine oxidase 저해활성은 75.6%로 높게 나타났다. 항고혈압 측정실험에서는 73.5%의 저해율을 나타내어 ACE 저해 활성이 뛰어난 것으로 나타났다. 이상의 결과는 산야의 우수한 생리활성을 증명하고 있고, 항산화력, 면역활성 및 항고혈압 효과가 높은 것으로 나타나 기능성 음료의 소재로서 그 활용도가 높을 것으로 판단된다. This study was investigated to analyze the contents of flavonoid compounds and the effects of fermentation on the physiological activities of medical plants, also known as SanYa (SY). Antioxidative activity of the fermented SanYa (FSY) was measured by using DPPH radical scavenging and SOD-like activity. DPPH radical scavenging and SOD-like activity of FSY were 94.3% and 45.0%, respectively. Nitric oxide (NO) synthesis was increased 11 times through the addition of FSY. However, NO production of the macrophages RAW264.7 cells stimulated with lipopolysaccharide (LPS) was reduced to 56% through the addition of FSY. FSY showed fibrinolytic activity and indicated about 69.8% and 73.7% of xanthine oxidase and angiotensin converting enzyme inhibitory activities, respectively. These results suggested that FSY plays a significant role in fibrinolytic activity and have strong xanthine oxidase and angiotensin converting enzyme inhibitory activities.